CV713173 | Citrus Genome Database

Overview


Name	CV713173
Unique Name	CV713173
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	739

Libraries


Library Name	Type
Parent Washington Navel Orange Callus cDNA Library UCRCS08-1	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CV713173 vs. ExPASy Swiss-Prot
Match: GSH1_MEDTR (Glutamate--cysteine ligase, chloroplastic OS=Medicago truncatula GN=GSH1 PE=2 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.072e-26
Identity = 32/37 (86.49%), Postives = 35/37 (94.59%), Query Frame = 1

Query:   34 TLVVFQDFLAGKLPCLPGELPTLNDWENHLTTIFPEV 144
            T + F+DFLAGKLPC+PGELPTLNDWENHLTTIFPEV
Sbjct:  336 TGMTFRDFLAGKLPCIPGELPTLNDWENHLTTIFPEV 372

HSP 2 Score: 66.2402 bits (160), Expect = 1.072e-26
Identity = 28/29 (96.55%), Postives = 29/29 (100.00%), Query Frame = 2

Query:  230 QVRLKRYLEMRGADGGPWRRLCALPAFWV 316
            +VRLKRYLEMRGADGGPWRRLCALPAFWV
Sbjct:  371 EVRLKRYLEMRGADGGPWRRLCALPAFWV 399

[Close]

BLAST of CV713173 vs. ExPASy Swiss-Prot
Match: GSH1_BRAJU (Glutamate--cysteine ligase, chloroplastic OS=Brassica juncea GN=GSH1 PE=1 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 1.947e-25
Identity = 31/37 (83.78%), Postives = 33/37 (89.19%), Query Frame = 1

Query:   34 TLVVFQDFLAGKLPCLPGELPTLNDWENHLTTIFPEV 144
            T + F+ FLAGKLPCLPGELPT NDWENHLTTIFPEV
Sbjct:  342 TGMTFRQFLAGKLPCLPGELPTYNDWENHLTTIFPEV 378

HSP 2 Score: 65.4698 bits (158), Expect = 1.947e-25
Identity = 27/29 (93.10%), Postives = 29/29 (100.00%), Query Frame = 2

Query:  230 QVRLKRYLEMRGADGGPWRRLCALPAFWV 316
            +VRLKRY+EMRGADGGPWRRLCALPAFWV
Sbjct:  377 EVRLKRYMEMRGADGGPWRRLCALPAFWV 405

[Close]

BLAST of CV713173 vs. ExPASy Swiss-Prot
Match: GSH1_ARATH (Glutamate--cysteine ligase, chloroplastic OS=Arabidopsis thaliana GN=GSH1 PE=1 SV=2)

HSP 1 Score: 69.707 bits (169), Expect = 3.297e-25
Identity = 30/37 (81.08%), Postives = 33/37 (89.19%), Query Frame = 1

Query:   34 TLVVFQDFLAGKLPCLPGELPTLNDWENHLTTIFPEV 144
            T + F+ FLAGKLPCLPGELP+ NDWENHLTTIFPEV
Sbjct:  350 TGMTFRQFLAGKLPCLPGELPSYNDWENHLTTIFPEV 386

HSP 2 Score: 66.2402 bits (160), Expect = 3.297e-25
Identity = 28/29 (96.55%), Postives = 29/29 (100.00%), Query Frame = 2

Query:  230 QVRLKRYLEMRGADGGPWRRLCALPAFWV 316
            +VRLKRYLEMRGADGGPWRRLCALPAFWV
Sbjct:  385 EVRLKRYLEMRGADGGPWRRLCALPAFWV 413

[Close]

BLAST of CV713173 vs. ExPASy Swiss-Prot
Match: GSH1B_ORYSJ (Glutamate--cysteine ligase B, chloroplastic OS=Oryza sativa subsp. japonica GN=GSH1-2 PE=3 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 4.598e-24
Identity = 28/29 (96.55%), Postives = 29/29 (100.00%), Query Frame = 2

Query:  230 QVRLKRYLEMRGADGGPWRRLCALPAFWV 316
            +VRLKRYLEMRGADGGPWRRLCALPAFWV
Sbjct:  359 EVRLKRYLEMRGADGGPWRRLCALPAFWV 387

HSP 2 Score: 65.855 bits (159), Expect = 4.598e-24
Identity = 28/33 (84.85%), Postives = 30/33 (90.91%), Query Frame = 1

Query:   46 FQDFLAGKLPCLPGELPTLNDWENHLTTIFPEV 144
            F+DF+ GKLP  PGELPTLNDWENHLTTIFPEV
Sbjct:  328 FRDFMVGKLPQAPGELPTLNDWENHLTTIFPEV 360

[Close]

BLAST of CV713173 vs. ExPASy Swiss-Prot
Match: GSH1B_ORYSI (Glutamate--cysteine ligase B, chloroplastic OS=Oryza sativa subsp. indica GN=GSH1-2 PE=3 SV=2)

HSP 1 Score: 66.2402 bits (160), Expect = 4.598e-24
Identity = 28/29 (96.55%), Postives = 29/29 (100.00%), Query Frame = 2

Query:  230 QVRLKRYLEMRGADGGPWRRLCALPAFWV 316
            +VRLKRYLEMRGADGGPWRRLCALPAFWV
Sbjct:  359 EVRLKRYLEMRGADGGPWRRLCALPAFWV 387

HSP 2 Score: 65.855 bits (159), Expect = 4.598e-24
Identity = 28/33 (84.85%), Postives = 30/33 (90.91%), Query Frame = 1

Query:   46 FQDFLAGKLPCLPGELPTLNDWENHLTTIFPEV 144
            F+DF+ GKLP  PGELPTLNDWENHLTTIFPEV
Sbjct:  328 FRDFMVGKLPQAPGELPTLNDWENHLTTIFPEV 360

[Close]

BLAST of CV713173 vs. ExPASy Swiss-Prot
Match: GSH1A_ORYSI (Glutamate--cysteine ligase A, chloroplastic OS=Oryza sativa subsp. indica GN=GSH1-1 PE=2 SV=2)

HSP 1 Score: 66.2402 bits (160), Expect = 4.599e-24
Identity = 28/29 (96.55%), Postives = 29/29 (100.00%), Query Frame = 2

Query:  230 QVRLKRYLEMRGADGGPWRRLCALPAFWV 316
            +VRLKRYLEMRGADGGPWRRLCALPAFWV
Sbjct:  355 EVRLKRYLEMRGADGGPWRRLCALPAFWV 383

HSP 2 Score: 65.855 bits (159), Expect = 4.599e-24
Identity = 28/33 (84.85%), Postives = 30/33 (90.91%), Query Frame = 1

Query:   46 FQDFLAGKLPCLPGELPTLNDWENHLTTIFPEV 144
            F+DF+ GKLP  PGELPTLNDWENHLTTIFPEV
Sbjct:  324 FRDFMVGKLPQAPGELPTLNDWENHLTTIFPEV 356

[Close]

BLAST of CV713173 vs. ExPASy Swiss-Prot
Match: GSH1_SOLLC (Glutamate--cysteine ligase, chloroplastic OS=Solanum lycopersicum GN=GSH1 PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.317e-23
Identity = 28/29 (96.55%), Postives = 29/29 (100.00%), Query Frame = 2

Query:  230 QVRLKRYLEMRGADGGPWRRLCALPAFWV 316
            +VRLKRYLEMRGADGGPWRRLCALPAFWV
Sbjct:  386 EVRLKRYLEMRGADGGPWRRLCALPAFWV 414

HSP 2 Score: 64.3142 bits (155), Expect = 1.317e-23
Identity = 27/33 (81.82%), Postives = 30/33 (90.91%), Query Frame = 1

Query:   46 FQDFLAGKLPCLPGELPTLNDWENHLTTIFPEV 144
            F+DF+ GKLP +PGE PTLNDWENHLTTIFPEV
Sbjct:  355 FRDFMNGKLPPIPGEYPTLNDWENHLTTIFPEV 387

[Close]

BLAST of CV713173 vs. ExPASy Swiss-Prot
Match: GSH1A_ORYSJ (Glutamate--cysteine ligase A, chloroplastic OS=Oryza sativa subsp. japonica GN=GSH1-1 PE=2 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 1.319e-23
Identity = 28/33 (84.85%), Postives = 30/33 (90.91%), Query Frame = 1

Query:   46 FQDFLAGKLPCLPGELPTLNDWENHLTTIFPEV 144
            F+DF+ GKLP  PGELPTLNDWENHLTTIFPEV
Sbjct:  324 FRDFMVGKLPQAPGELPTLNDWENHLTTIFPEV 356

HSP 2 Score: 64.6994 bits (156), Expect = 1.319e-23
Identity = 27/29 (93.10%), Postives = 28/29 (96.55%), Query Frame = 2

Query:  230 QVRLKRYLEMRGADGGPWRRLCALPAFWV 316
            +VRLKRYLEMRGADGGPWRRLCALP FWV
Sbjct:  355 EVRLKRYLEMRGADGGPWRRLCALPVFWV 383

[Close]

BLAST of CV713173 vs. ExPASy Swiss-Prot
Match: GSH1_TOBAC (Glutamate--cysteine ligase, chloroplastic OS=Nicotiana tabacum GN=GSH1 PE=2 SV=2)

HSP 1 Score: 66.2402 bits (160), Expect = 2.383e-22
Identity = 28/29 (96.55%), Postives = 29/29 (100.00%), Query Frame = 2

Query:  230 QVRLKRYLEMRGADGGPWRRLCALPAFWV 316
            +VRLKRYLEMRGADGGPWRRLCALPAFWV
Sbjct:  385 EVRLKRYLEMRGADGGPWRRLCALPAFWV 413

HSP 2 Score: 60.077 bits (144), Expect = 2.383e-22
Identity = 25/33 (75.76%), Postives = 29/33 (87.88%), Query Frame = 1

Query:   46 FQDFLAGKLPCLPGELPTLNDWENHLTTIFPEV 144
            F+DF+ GKL  +PG+ PTLNDWENHLTTIFPEV
Sbjct:  354 FRDFMNGKLSPIPGDYPTLNDWENHLTTIFPEV 386

The following BLAST results are available for this feature:

BLAST of CV713173 vs. ExPASy Swiss-Prot

BLAST of CV713173 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 9


Match Name	E-value	Identity	Description
GSH1_MEDTR	1.072e-26	86.49	Glutamate--cysteine ligase, chloroplastic OS=Medic...	[more]
GSH1_BRAJU	1.947e-25	83.78	Glutamate--cysteine ligase, chloroplastic OS=Brass...	[more]
GSH1_ARATH	3.297e-25	81.08	Glutamate--cysteine ligase, chloroplastic OS=Arabi...	[more]
GSH1B_ORYSJ	4.598e-24	96.55	Glutamate--cysteine ligase B, chloroplastic OS=Ory...	[more]
GSH1B_ORYSI	4.598e-24	96.55	Glutamate--cysteine ligase B, chloroplastic OS=Ory...	[more]
GSH1A_ORYSI	4.599e-24	96.55	Glutamate--cysteine ligase A, chloroplastic OS=Ory...	[more]
GSH1_SOLLC	1.317e-23	96.55	Glutamate--cysteine ligase, chloroplastic OS=Solan...	[more]
GSH1A_ORYSJ	1.319e-23	84.85	Glutamate--cysteine ligase A, chloroplastic OS=Ory...	[more]
GSH1_TOBAC	2.383e-22	96.55	Glutamate--cysteine ligase, chloroplastic OS=Nicot...	[more]

Properties


Property Name	Value
Genbank description	UCRCS08_0002G22_r Parent Washington Navel Orange Callus cDNA Library UCRCS08-1 Citrus sinensis cDNA clone CS_AEa0002G22, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CV713173 ID=CV713173; Name=CV713173; organism=Citrus sinensis; type=EST; length=739bp
ATTGTATCTCACGCTATTAATTTTTGTACAAAAACTCTCGTCGTTTTTCA
GGACTTCTTGGCTGGAAAACTTCCGTGCCTTCCTGGTGAATTGCCAACTC
TTAATGATTGGGAAAATCATCTGACAACAATATTTCCCGAGGTCTGTTTT
ATGTGTTTTTGATCAATTTGCTGCAAATTTATGCTCACAATGCCTATGCC
AACTTTGAGCCACATTTTAATCTTACATTCAGGTTAGGTTGAAGAGATAC
TTGGAGATGAGAGGCGCTGATGGAGGGCCTTGGAGGAGGTTATGTGCTTT
GCCTGCATTTTGGGTAGTGGCATCCTTATTCTGTTTCTGTATAATCTCTA
TATTGTGCATGGTTTGGTGGTAGTCAGTTGGATAGCATGTTCTAGTAACG
CTAGAGAATAAATATGCTTAAGAAAGAAGGATGGGGGAATTAAAAGGATA
TATTTTTGAACTTTTTGAATAATGATACATGATGTTACTAATCTCTGTGC
ATTTGATAGCCAATGGGGAACTCTTGGACACATGTCCCTGCTTCTTTATA
TGAAGTCTTAATGATAAAATAAACCTCCTCACAAAACTTTTTTTTATGTA
CATATCAATGGCCTCTAGCTTATAGTTTTGTGACGTTCTGTCAGAAATAT
CAGTTGCACGTTTCCCTCTTGTCACTTTTTGTCTAGCCCTTGCAGATTAT
GTAGCTTCTTGTTTTTTTCTTTTATGTATATTTTTAAAA