CV712847 | Citrus Genome Database

Overview


Name	CV712847
Unique Name	CV712847
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	878

Libraries


Library Name	Type
Parent Washington Navel Orange Callus cDNA Library UCRCS08-1	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CV712847 vs. ExPASy Swiss-Prot
Match: ISPH_SYNP6 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) GN=ispH PE=3 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 8.082e-24
Identity = 39/67 (58.21%), Postives = 54/67 (80.60%), Query Frame = 2

Query:  416 QERQDAMY*LVEEKIDLILLVGRWNSSTTSSLQKIAEDRGISSYWIDSEKRIGPGNKIAHKLMHGEL 616
            QERQDAM+ LVEE +DLI+++G +NSS T+ LQ+IA +R I S+ ID+ +RIGPGN+I HK +H +L
Sbjct:  292 QERQDAMFQLVEEPLDLIVVIGGFNSSNTTHLQEIAIERQIPSFHIDAAERIGPGNRIEHKPLHTDL 358

HSP 2 Score: 50.8322 bits (120), Expect = 8.082e-24
Identity = 19/34 (55.88%), Postives = 30/34 (88.24%), Query Frame = 1

Query:  631 WLPKGQITIGVTAGASIPDKAVENVLKKVFEIER 732
            WLP G +TIG+T+GAS PDK VE+V++++F+++R
Sbjct:  364 WLPAGPLTIGITSGASTPDKVVEDVIERLFDLQR 397

[Close]

BLAST of CV712847 vs. ExPASy Swiss-Prot
Match: ISPH_SYNE7 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus elongatus (strain PCC 7942) GN=ispH PE=3 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 8.082e-24
Identity = 39/67 (58.21%), Postives = 54/67 (80.60%), Query Frame = 2

Query:  416 QERQDAMY*LVEEKIDLILLVGRWNSSTTSSLQKIAEDRGISSYWIDSEKRIGPGNKIAHKLMHGEL 616
            QERQDAM+ LVEE +DLI+++G +NSS T+ LQ+IA +R I S+ ID+ +RIGPGN+I HK +H +L
Sbjct:  292 QERQDAMFQLVEEPLDLIVVIGGFNSSNTTHLQEIAIERQIPSFHIDAAERIGPGNRIEHKPLHTDL 358

HSP 2 Score: 50.8322 bits (120), Expect = 8.082e-24
Identity = 19/34 (55.88%), Postives = 30/34 (88.24%), Query Frame = 1

Query:  631 WLPKGQITIGVTAGASIPDKAVENVLKKVFEIER 732
            WLP G +TIG+T+GAS PDK VE+V++++F+++R
Sbjct:  364 WLPAGPLTIGITSGASTPDKVVEDVIERLFDLQR 397

[Close]

BLAST of CV712847 vs. ExPASy Swiss-Prot
Match: ISPH_CYAA5 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain ATCC 51142) GN=ispH PE=3 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 3.015e-23
Identity = 38/71 (53.52%), Postives = 56/71 (78.87%), Query Frame = 2

Query:  416 QERQDAMY*LVEEKIDLILLVGRWNSSTTSSLQKIAEDRGISSYWIDSEKRIGPGNKIAHKLMHGELVEKK 628
            QERQDAM  LVEE++D+++++G +NSS T+ LQ+I+ ++GI SY IDS+KRI PGNKI HK +  ++  K+
Sbjct:  292 QERQDAMLNLVEEEVDVMVVIGGFNSSNTTHLQEISIEKGIDSYHIDSDKRILPGNKIEHKPLEKDIETKE 362

HSP 2 Score: 50.447 bits (119), Expect = 3.015e-23
Identity = 20/34 (58.82%), Postives = 29/34 (85.29%), Query Frame = 1

Query:  622 EKNWLPKGQITIGVTAGASIPDKAVENVLKKVFE 723
            ++NWLP G+I +GVT+GAS PDK VE V++++FE
Sbjct:  361 KENWLPDGKIIVGVTSGASTPDKVVEAVIERIFE 394

[Close]

BLAST of CV712847 vs. ExPASy Swiss-Prot
Match: ISPH_THEEB (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Thermosynechococcus elongatus (strain BP-1) GN=ispH PE=3 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 1.124e-22
Identity = 38/64 (59.38%), Postives = 50/64 (78.12%), Query Frame = 2

Query:  416 QERQDAMY*LVEEKIDLILLVGRWNSSTTSSLQKIAEDRGISSYWIDSEKRIGPGNKIAHKLMH 607
            QERQDAM  LV+E +DL++++G +NSS T+ LQ+IA + GI SY IDS  RIGPGN+I HK +H
Sbjct:  292 QERQDAMLSLVKEPLDLMVVIGGYNSSNTTHLQEIAIEHGIPSYHIDSADRIGPGNRIEHKPLH 355

HSP 2 Score: 49.2914 bits (116), Expect = 1.124e-22
Identity = 21/34 (61.76%), Postives = 26/34 (76.47%), Query Frame = 1

Query:  625 KNWLPKGQITIGVTAGASIPDKAVENVLKKVFEI 726
            +NWLP   ITIG+T+GAS PDK VE VL K+F +
Sbjct:  362 ENWLPDRPITIGITSGASTPDKVVEEVLNKIFAL 395

[Close]

BLAST of CV712847 vs. ExPASy Swiss-Prot
Match: ISPH_CYAP4 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) GN=ispH PE=3 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.903e-22
Identity = 38/71 (53.52%), Postives = 53/71 (74.65%), Query Frame = 2

Query:  416 QERQDAMY*LVEEKIDLILLVGRWNSSTTSSLQKIAEDRGISSYWIDSEKRIGPGNKIAHKLMHGELVEKK 628
            QERQDAM+ LVE  +DL++++G +NSS T+ LQ+I+ +R I SY IDS  RIGPGN++ HK +H  L  K+
Sbjct:  293 QERQDAMFKLVEYPLDLMVVIGGFNSSNTTHLQEISIEREIPSYHIDSADRIGPGNRVEHKPLHRPLEIKE 363

HSP 2 Score: 50.447 bits (119), Expect = 1.903e-22
Identity = 19/36 (52.78%), Postives = 30/36 (83.33%), Query Frame = 1

Query:  622 EKNWLPKGQITIGVTAGASIPDKAVENVLKKVFEIE 729
            ++NWLP+G I +G+T+GAS PDK V +VL+K+F ++
Sbjct:  362 KENWLPEGPIVVGITSGASTPDKVVSDVLEKIFALK 397

[Close]

BLAST of CV712847 vs. ExPASy Swiss-Prot
Match: ISPH_ACAM1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Acaryochloris marina (strain MBIC 11017) GN=ispH PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 7.089e-22
Identity = 35/67 (52.24%), Postives = 53/67 (79.10%), Query Frame = 2

Query:  416 QERQDAMY*LVEEKIDLILLVGRWNSSTTSSLQKIAEDRGISSYWIDSEKRIGPGNKIAHKLMHGEL 616
            QERQDA++ LV+E +DL++++G +NSS T+ LQ+I+ D+ I SY IDS  RIGPGN++ HK ++ +L
Sbjct:  301 QERQDAIFQLVDEPLDLMVVIGGFNSSNTTHLQEISIDKNIPSYHIDSVDRIGPGNRVEHKPLNADL 367

HSP 2 Score: 48.9062 bits (115), Expect = 7.089e-22
Identity = 19/34 (55.88%), Postives = 27/34 (79.41%), Query Frame = 1

Query:  628 NWLPKGQITIGVTAGASIPDKAVENVLKKVFEIE 729
            NWLP G + IGVT+GAS PDK V ++++K+F I+
Sbjct:  372 NWLPNGPLAIGVTSGASTPDKVVSDIVEKIFSIK 405

[Close]

BLAST of CV712847 vs. ExPASy Swiss-Prot
Match: ISPH_ANAVT (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Anabaena variabilis (strain ATCC 29413 / PCC 7937) GN=ispH PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 9.230e-22
Identity = 35/68 (51.47%), Postives = 52/68 (76.47%), Query Frame = 2

Query:  416 QERQDAMY*LVEEKIDLILLVGRWNSSTTSSLQKIAEDRGISSYWIDSEKRIGPGNKIAHKLMHGELV 619
            QERQDAM  LV+E +DL++++G +NSS T+ LQ+I+++RG+ SY ID  +RI   N I H+ ++GELV
Sbjct:  292 QERQDAMLELVQENLDLMIVIGGFNSSNTTQLQQISQERGLPSYHIDVVERIKSVNSIEHRQLNGELV 359

HSP 2 Score: 51.6026 bits (122), Expect = 9.230e-22
Identity = 20/35 (57.14%), Postives = 30/35 (85.71%), Query Frame = 1

Query:  625 KNWLPKGQITIGVTAGASIPDKAVENVLKKVFEIE 729
            +NWLP G+I +GVT+GAS PDK VE+V++K+F ++
Sbjct:  362 ENWLPAGKIVVGVTSGASTPDKVVEDVIEKIFALK 396

[Close]

BLAST of CV712847 vs. ExPASy Swiss-Prot
Match: ISPH_ANASP (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Anabaena sp. (strain PCC 7120) GN=ispH PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.201e-21
Identity = 35/68 (51.47%), Postives = 52/68 (76.47%), Query Frame = 2

Query:  416 QERQDAMY*LVEEKIDLILLVGRWNSSTTSSLQKIAEDRGISSYWIDSEKRIGPGNKIAHKLMHGELV 619
            QERQDAM  LV+E +DL++++G +NSS T+ LQ+I+++RG+ SY ID  +RI   N I H+ ++GELV
Sbjct:  292 QERQDAMLELVQENLDLMIVIGGFNSSNTTQLQQISQERGLPSYHIDVVERIKSINSIEHRQLNGELV 359

HSP 2 Score: 51.6026 bits (122), Expect = 1.201e-21
Identity = 20/35 (57.14%), Postives = 30/35 (85.71%), Query Frame = 1

Query:  625 KNWLPKGQITIGVTAGASIPDKAVENVLKKVFEIE 729
            +NWLP G+I +GVT+GAS PDK VE+V++K+F ++
Sbjct:  362 ENWLPAGKIVVGVTSGASTPDKVVEDVIEKIFALK 396

[Close]

BLAST of CV712847 vs. ExPASy Swiss-Prot
Match: ISPH_SYNY3 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechocystis sp. (strain PCC 6803) GN=ispH PE=3 SV=2)

HSP 1 Score: 73.1738 bits (178), Expect = 2.034e-21
Identity = 36/67 (53.73%), Postives = 50/67 (74.63%), Query Frame = 2

Query:  416 QERQDAMY*LVEEKIDLILLVGRWNSSTTSSLQKIAEDRGISSYWIDSEKRIGPGNKIAHKLMHGEL 616
            QERQDAM+ LVEE + L++++G +NSS T+ LQ+IA + GI S  IDS  RIGPGN++ HK +  +L
Sbjct:  265 QERQDAMFDLVEEDLSLMVVIGGFNSSNTTHLQEIAVEHGIPSVHIDSGDRIGPGNRVEHKPLGKDL 331

HSP 2 Score: 50.447 bits (119), Expect = 2.034e-21
Identity = 21/33 (63.64%), Postives = 27/33 (81.82%), Query Frame = 1

Query:  631 WLPKGQITIGVTAGASIPDKAVENVLKKVFEIE 729
            WLP G+IT+GVT+GAS PDK VE V+KK+  I+
Sbjct:  337 WLPAGKITVGVTSGASTPDKVVEEVMKKILAIK 369

[Close]

BLAST of CV712847 vs. ExPASy Swiss-Prot
Match: ISPH_NOSP7 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) GN=ispH PE=3 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 2.167e-20
Identity = 33/68 (48.53%), Postives = 51/68 (75.00%), Query Frame = 2

Query:  416 QERQDAMY*LVEEKIDLILLVGRWNSSTTSSLQKIAEDRGISSYWIDSEKRIGPGNKIAHKLMHGELV 619
            QERQDAM  LVE  ++L++++G +NSS T+ LQ+IA +R I SY ID+ +RI  G+ I H+ ++G+L+
Sbjct:  292 QERQDAMLELVEHNLNLMVVIGGFNSSNTTQLQQIAFERRIPSYHIDTVERIKSGDSIEHRQLNGQLI 359

HSP 2 Score: 51.9878 bits (123), Expect = 2.167e-20
Identity = 19/35 (54.29%), Postives = 30/35 (85.71%), Query Frame = 1

Query:  625 KNWLPKGQITIGVTAGASIPDKAVENVLKKVFEIE 729
            +NWLP G+I +G+T+GAS PDK VE+V++K+F ++
Sbjct:  362 ENWLPDGEIVVGITSGASTPDKVVEDVIEKIFAVK 396

The following BLAST results are available for this feature:

BLAST of CV712847 vs. ExPASy Swiss-Prot

BLAST of CV712847 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 33


Match Name	E-value	Identity	Description
ISPH_SYNP6	8.082e-24	58.21	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]
ISPH_SYNE7	8.082e-24	58.21	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]
ISPH_CYAA5	3.015e-23	53.52	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]
ISPH_THEEB	1.124e-22	59.38	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]
ISPH_CYAP4	1.903e-22	53.52	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]
ISPH_ACAM1	7.089e-22	52.24	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]
ISPH_ANAVT	9.230e-22	51.47	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]
ISPH_ANASP	1.201e-21	51.47	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]
ISPH_SYNY3	2.034e-21	53.73	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]
ISPH_NOSP7	2.167e-20	48.53	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]

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Properties


Property Name	Value
Genbank description	UCRCS08_0001P17_f Parent Washington Navel Orange Callus cDNA Library UCRCS08-1 Citrus sinensis cDNA clone CS_AEa0001P17, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CV712847 ID=CV712847; Name=CV712847; organism=Citrus sinensis; type=EST; length=878bp
TGGCTTCTGGTATTTGAGCAATTTTTCATTGTTACAGGAAAATTAGTAGA
GAAGACCATGATGCGTAAATTTGGAGTTGAAAATGTCAATGAACACTTCA
TAAGCTTCAACACAATTTGTTATGCTGCACAAGTAATTTCTTCTTCTAAC
TCATGGCTTTTCCTTTTCAGATTTTTATAATTTTGTGTGTCATAGAACTT
CCTGTCCACTGCATGTCTTTTAACGATCTTTATAAAACCCAGAATTTTGA
ACCTCGATATGCCAAACCTAACAACATACTTGACCATTTGTTTTCAATGA
CCGAACTGCTCCGCCCGCAAGAGTGCAATACTCAGCCACTAGTTAAACAG
CTAGTTGTGCAACATTCAGTTGCGTTCAGTAAAATTTAAGCTAAAGATTT
TGGTTTCAAGTTTTGCAGGAGCGGCAAGATGCAATGTATTAGTTGGTTGA
GGAAAAGATCGATCTTATTTTGCTTGTTGGCAGGTGGAACTCGAGCACTA
CCTCAAGCCTTCAAAAGATTGCAGAAGACCGTGGAATCTCCTCATACTGG
ATCGACAGTGAGAAAAGAATAGGTCCCGGAAACAAAATAGCACATAAATT
AATGCATGGTGAGCTGGTGGAGAAAAAAATTGGCTGCCGAAAGGACAGAT
CACAATCGGTGTAACAGCCGGTGCCTCCATCCCAGATAAGGCAGTTGAAA
ATGTCCTTAAAAAGGTGTTCGAGATCGAGCGTGAAGAAGCCTTGNCATTG
GCGTAAACTGAGGTCCAAACGTACATATTATCAATAAATTTCAATGTGAT
TCTCAATTAGATATTGTAAAATTATAAACACAACCCTCTTCTGAGTCTTT
TCTTAGTGCCCATGTGATCTGAAATTTT