CN192062 | Citrus Genome Database

Overview


Name	CN192062
Unique Name	CN192062
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	637

Libraries


Library Name	Type
Washington Navel Orange Stored Fruit Rind cDNA Library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig5994	contig	Csv1_Contig5994:265..900.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CN192062 vs. ExPASy Swiss-Prot
Match: UBC20_ARATH (Ubiquitin carrier protein E2 20 OS=Arabidopsis thaliana GN=UBC20 PE=2 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 1.234e-35
Identity = 71/89 (79.78%), Postives = 77/89 (86.52%), Query Frame = -3

Query:  279 SLRFPLDYPFKPPQVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALLNNKEDYRKMVQKQY 545
            SL F  DYPFKPP+VKFET CFHPNVD YGNICLDILQDKWSSAYD RTILLSIQSLLGEPN  SPLN+ AA L +N+E+YRKMV+K Y
Sbjct:   87 SLSFSNDYPFKPPKVKFETCCFHPNVDVYGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLY 175

HSP 2 Score: 21.557 bits (44), Expect = 1.234e-35
Identity = 8/13 (61.54%), Postives = 10/13 (76.92%), Query Frame = -1

Query:  563 GTIEGGKGTMYEG 601
            GTI G K T++EG
Sbjct:   69 GTITGSKDTVFEG 81

[Close]

BLAST of CN192062 vs. ExPASy Swiss-Prot
Match: UBE2C_DICDI (Probable ubiquitin-conjugating enzyme E2 C OS=Dictyostelium discoideum GN=ube2c PE=3 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 7.893e-35
Identity = 62/89 (69.66%), Postives = 78/89 (87.64%), Query Frame = -3

Query:  279 SLRFPLDYPFKPPQVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALLNNKEDYRKMVQKQY 545
            SL+FP DYP+KPP VKFET CFHPNVD  GNICLDIL+DKWS  Y+ R++L+SIQSLLGEPN ESPLNSYAA+L +N+++Y+K++ K+Y
Sbjct:   58 SLKFPTDYPYKPPTVKFETQCFHPNVDNNGNICLDILKDKWSPVYNVRSLLISIQSLLGEPNNESPLNSYAASLWSNQDEYKKVLDKRY 146

HSP 2 Score: 25.409 bits (54), Expect = 7.893e-35
Identity = 10/16 (62.50%), Postives = 13/16 (81.25%), Query Frame = -1

Query:  566 FHLIGTIEGGKGTMYE 613
            F+ IGTI+G K T+YE
Sbjct:   36 FNWIGTIQGAKDTVYE 51

[Close]

BLAST of CN192062 vs. ExPASy Swiss-Prot
Match: UBC19_ARATH (Ubiquitin carrier protein E2 19 OS=Arabidopsis thaliana GN=UBC19 PE=1 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 1.023e-34
Identity = 70/89 (78.65%), Postives = 76/89 (85.39%), Query Frame = -3

Query:  279 SLRFPLDYPFKPPQVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALLNNKEDYRKMVQKQY 545
            SL F  DYPFK P+VKFET CFHPNVD YGNICLDILQDKWSSAYD RTILLSIQSLLGEPN  SPLN+ AA L +N+E+YRKMV+K Y
Sbjct:   88 SLTFSNDYPFKSPKVKFETCCFHPNVDLYGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNNQAAQLWSNQEEYRKMVEKLY 176

HSP 2 Score: 21.557 bits (44), Expect = 1.023e-34
Identity = 8/13 (61.54%), Postives = 10/13 (76.92%), Query Frame = -1

Query:  563 GTIEGGKGTMYEG 601
            GTI G K T++EG
Sbjct:   70 GTITGSKDTVFEG 82

[Close]

BLAST of CN192062 vs. ExPASy Swiss-Prot
Match: UBE2C_XENLA (Ubiquitin-conjugating enzyme E2 C OS=Xenopus laevis GN=ube2c PE=1 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 1.061e-30
Identity = 60/89 (67.42%), Postives = 69/89 (77.53%), Query Frame = -3

Query:  279 SLRFPLDYPFKPPQVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALLNNKEDYRKMVQKQY 545
            SL FP  YP+  P VKF T CFHPNVD +GNICLDIL+DKWS+ YD RTILLS+QSLLGEPN ESPLN YAA L  N+  Y+K + +QY
Sbjct:   82 SLEFPSGYPYNAPTVKFVTPCFHPNVDSHGNICLDILKDKWSALYDVRTILLSLQSLLGEPNNESPLNPYAAELWQNQTAYKKHLHEQY 170

HSP 2 Score: 24.6386 bits (52), Expect = 1.061e-30
Identity = 10/16 (62.50%), Postives = 12/16 (75.00%), Query Frame = -1

Query:  566 FHLIGTIEGGKGTMYE 613
            F  IGTI+G  GT+YE
Sbjct:   60 FKWIGTIDGAVGTVYE 75

[Close]

BLAST of CN192062 vs. ExPASy Swiss-Prot
Match: UBE2C_SPISO (Ubiquitin-conjugating enzyme E2 C OS=Spisula solidissima GN=UBE2C PE=1 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 1.269e-28
Identity = 55/89 (61.80%), Postives = 74/89 (83.15%), Query Frame = -3

Query:  279 SLRFPLDYPFKPPQVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALLNNKEDYRKMVQKQY 545
            +L FP DYP+KPP VKF T C+HPNVDQ GNICLDIL++ W+++YD RTILLS+QSLLGEPN  SPLN+ AA + +N+ +Y+K++ ++Y
Sbjct:   82 TLEFPSDYPYKPPVVKFTTPCWHPNVDQSGNICLDILKENWTASYDVRTILLSLQSLLGEPNNASPLNAQAADMWSNQTEYKKVLHEKY 170

[Close]

BLAST of CN192062 vs. ExPASy Swiss-Prot
Match: UBE2C_MOUSE (Ubiquitin-conjugating enzyme E2 C OS=Mus musculus GN=Ube2c PE=2 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 2.711e-28
Identity = 57/89 (64.04%), Postives = 68/89 (76.40%), Query Frame = -3

Query:  279 SLRFPLDYPFKPPQVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALLNNKEDYRKMVQKQY 545
            SL FP  YP+  P VKF T C+HPNVD  GNICLDIL+DKWS+ YD RTILLSIQSLLGEPN +SPLN++AA L  N   ++K +Q+ Y
Sbjct:   82 SLEFPSGYPYNAPTVKFLTPCYHPNVDTQGNICLDILKDKWSALYDVRTILLSIQSLLGEPNIDSPLNTHAAELWKNPTAFKKYLQETY 170

HSP 2 Score: 23.8682 bits (50), Expect = 2.711e-28
Identity = 9/16 (56.25%), Postives = 11/16 (68.75%), Query Frame = -1

Query:  566 FHLIGTIEGGKGTMYE 613
            F  +GTI G  GT+YE
Sbjct:   60 FKWVGTIHGAAGTVYE 75

[Close]

BLAST of CN192062 vs. ExPASy Swiss-Prot
Match: UBE2C_MACFA (Ubiquitin-conjugating enzyme E2 C OS=Macaca fascicularis GN=UBE2C PE=2 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 2.711e-28
Identity = 57/89 (64.04%), Postives = 68/89 (76.40%), Query Frame = -3

Query:  279 SLRFPLDYPFKPPQVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALLNNKEDYRKMVQKQY 545
            SL FP  YP+  P VKF T C+HPNVD  GNICLDIL+DKWS+ YD RTILLSIQSLLGEPN +SPLN++AA L  N   ++K +Q+ Y
Sbjct:   82 SLEFPSGYPYNAPTVKFLTPCYHPNVDTQGNICLDILKDKWSALYDVRTILLSIQSLLGEPNIDSPLNTHAAELWKNPTAFKKYLQETY 170

HSP 2 Score: 23.8682 bits (50), Expect = 2.711e-28
Identity = 9/16 (56.25%), Postives = 11/16 (68.75%), Query Frame = -1

Query:  566 FHLIGTIEGGKGTMYE 613
            F  +GTI G  GT+YE
Sbjct:   60 FKWVGTIHGAAGTVYE 75

[Close]

BLAST of CN192062 vs. ExPASy Swiss-Prot
Match: UBE2C_BOVIN (Ubiquitin-conjugating enzyme E2 C OS=Bos taurus GN=UBE2C PE=2 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 2.711e-28
Identity = 57/89 (64.04%), Postives = 68/89 (76.40%), Query Frame = -3

Query:  279 SLRFPLDYPFKPPQVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALLNNKEDYRKMVQKQY 545
            SL FP  YP+  P VKF T C+HPNVD  GNICLDIL+DKWS+ YD RTILLSIQSLLGEPN +SPLN++AA L  N   ++K +Q+ Y
Sbjct:   82 SLEFPSGYPYNAPTVKFLTPCYHPNVDTQGNICLDILKDKWSALYDVRTILLSIQSLLGEPNIDSPLNTHAAELWKNPTAFKKYLQETY 170

HSP 2 Score: 23.8682 bits (50), Expect = 2.711e-28
Identity = 9/16 (56.25%), Postives = 11/16 (68.75%), Query Frame = -1

Query:  566 FHLIGTIEGGKGTMYE 613
            F  +GTI G  GT+YE
Sbjct:   60 FKWVGTIHGAAGTVYE 75

[Close]

BLAST of CN192062 vs. ExPASy Swiss-Prot
Match: UBE2C_HUMAN (Ubiquitin-conjugating enzyme E2 C OS=Homo sapiens GN=UBE2C PE=1 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 7.790e-28
Identity = 56/89 (62.92%), Postives = 68/89 (76.40%), Query Frame = -3

Query:  279 SLRFPLDYPFKPPQVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALLNNKEDYRKMVQKQY 545
            SL FP  YP+  P VKF T C+HPNVD  GNICLDIL++KWS+ YD RTILLSIQSLLGEPN +SPLN++AA L  N   ++K +Q+ Y
Sbjct:   82 SLEFPSGYPYNAPTVKFLTPCYHPNVDTQGNICLDILKEKWSALYDVRTILLSIQSLLGEPNIDSPLNTHAAELWKNPTAFKKYLQETY 170

HSP 2 Score: 23.8682 bits (50), Expect = 7.790e-28
Identity = 9/16 (56.25%), Postives = 11/16 (68.75%), Query Frame = -1

Query:  566 FHLIGTIEGGKGTMYE 613
            F  +GTI G  GT+YE
Sbjct:   60 FKWVGTIHGAAGTVYE 75

[Close]

BLAST of CN192062 vs. ExPASy Swiss-Prot
Match: UBE2C_DROME (Ubiquitin-conjugating enzyme E2 C OS=Drosophila melanogaster GN=vih PE=1 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 4.940e-27
Identity = 57/89 (64.04%), Postives = 68/89 (76.40%), Query Frame = -3

Query:  279 SLRFPLDYPFKPPQVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALLNNKEDYRKMVQKQY 545
            SL FP  YP+  P VKF T CFHPNVD  G ICLDIL+DKWS+ YD RTILLSIQSLLGEPN ESPLN+ AA + N++++Y+K +   Y
Sbjct:   84 SLDFPNSYPYAAPVVKFLTSCFHPNVDLQGAICLDILKDKWSALYDVRTILLSIQSLLGEPNNESPLNAQAAMMWNDQKEYKKYLDAFY 172

HSP 2 Score: 21.9422 bits (45), Expect = 4.940e-27
Identity = 8/17 (47.06%), Postives = 11/17 (64.71%), Query Frame = -1

Query:  563 FHLIGTIEGGKGTMYEG 613
            F  +GTI G + T+Y G
Sbjct:   62 FKWVGTIAGPRNTVYSG 78

The following BLAST results are available for this feature:

BLAST of CN192062 vs. ExPASy Swiss-Prot

BLAST of CN192062 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 188


Match Name	E-value	Identity	Description
UBC20_ARATH	1.234e-35	79.78	Ubiquitin carrier protein E2 20 OS=Arabidopsis tha...	[more]
UBE2C_DICDI	7.893e-35	69.66	Probable ubiquitin-conjugating enzyme E2 C OS=Dict...	[more]
UBC19_ARATH	1.023e-34	78.65	Ubiquitin carrier protein E2 19 OS=Arabidopsis tha...	[more]
UBE2C_XENLA	1.061e-30	67.42	Ubiquitin-conjugating enzyme E2 C OS=Xenopus laevi...	[more]
UBE2C_SPISO	1.269e-28	61.80	Ubiquitin-conjugating enzyme E2 C OS=Spisula solid...	[more]
UBE2C_MOUSE	2.711e-28	64.04	Ubiquitin-conjugating enzyme E2 C OS=Mus musculus ...	[more]
UBE2C_MACFA	2.711e-28	64.04	Ubiquitin-conjugating enzyme E2 C OS=Macaca fascic...	[more]
UBE2C_BOVIN	2.711e-28	64.04	Ubiquitin-conjugating enzyme E2 C OS=Bos taurus GN...	[more]
UBE2C_HUMAN	7.790e-28	62.92	Ubiquitin-conjugating enzyme E2 C OS=Homo sapiens ...	[more]
UBE2C_DROME	4.940e-27	64.04	Ubiquitin-conjugating enzyme E2 C OS=Drosophila me...	[more]

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Properties


Property Name	Value
Genbank description	UCRCS06_0006E16_r Washington Navel Orange Stored Fruit Rind cDNA Library Citrus sinensis cDNA clone CS_WEc0006E16, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CN192062 ID=CN192062; Name=CN192062; organism=Citrus sinensis; type=EST; length=637bp
GCCCTTTCATTTGATTCAAACAAAATTACAACACCGTGTAAACTTTATTT
TGCTATTACATGAGAAATTATCGAGAGCCTGAGTAACCTTCCTTTGGGTA
TAATGTAAATAACTGTACTGACAATGATGAGACTTATGTAGATCAGTTTT
CATTGAAACAAGTTCCAAACTGAAACAAGCCCTTCCCCTTGAGCTTTACA
GAGAAATAATGTGGAAGCCTAACATCCGATTTTGTATCTGTTTTGCTGTT
CAAATTAGCTCTCAAATTCTCCAGCAAAGTACTGTTTCTGGACCATTTTT
CTGTAATCTTCCTTATTGTTCAACAGAGCTGCAGCATAGCTGTTGAGAGG
GCTCTCTGGATTAGGCTCTCCCAGTAAACTTTGAATGGACAAAAGAATCG
TTCTGCAATCATACGCTGAAGACCACTTGTCCTGAAGGATGTCTAGGCAT
ATGTTGCCATACTGATCAACATTTGGATGGAAGCACATAGTTTCAAACTT
GACTTGGGGTGGCTTGAAAGGATAATCCAGAGGAAAACGCAAAGAAAAGT
TTGTATGATAAAACCCTCATACATAGTTCCCTTTCCACCCTCGATTGTGC
CAATCAAGTGAAAATGCTCTCGCCTTCAGGAAAGGCC