CN191807

Overview
NameCN191807
Unique NameCN191807
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length707
Libraries
Library NameType
Washington Navel Orange Stored Fruit Rind cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN191807 vs. ExPASy Swiss-Prot
Match: VPE_CITSI (Vacuolar-processing enzyme OS=Citrus sinensis PE=2 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 3.054e-58
Identity = 69/71 (97.18%), Postives = 70/71 (98.59%), Query Frame = -2 
Query:  326 IVDDWGCLKSLVRTFESHCGALSQYGMKHMRSLANICNTGIGKEKMAEASAQACENIPFGPWSSLDKGFSA 538
            +VDDWGCLKSLVRTFESHCGALSQYGMKHMRSLANICNTGIGKEKMAEASAQACENIP GPWSSLDKGFSA
Sbjct:  424 LVDDWGCLKSLVRTFESHCGALSQYGMKHMRSLANICNTGIGKEKMAEASAQACENIPSGPWSSLDKGFSA 494

HSP 2 Score: 97.8265 bits (242), Expect = 3.054e-58
Identity = 47/49 (95.92%), Postives = 47/49 (95.92%), Query Frame = -3 
Query:  559 APEGTPRKVEAQKQFFEAMSHGMHVDHSIKLIGKLLFGIEKGPEILNTV 705
            APEGTPRK EAQKQFFEAMSH MHVDHSIKLIGKLLFGIEKGPEILNTV
Sbjct:  369 APEGTPRKAEAQKQFFEAMSHRMHVDHSIKLIGKLLFGIEKGPEILNTV 417
[Close]
BLAST of CN191807 vs. ExPASy Swiss-Prot
Match: VPE1_PHAVU (Vacuolar-processing enzyme OS=Phaseolus vulgaris PE=1 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 2.151e-43
Identity = 54/73 (73.97%), Postives = 62/73 (84.93%), Query Frame = -2 
Query:  326 SLIVDDWGCLKSLVRTFESHCGALSQYGMKHMRSLANICNTGIGKEKMAEASAQACENIPFGPWSSLDKGFSA 544
            S +VDDW CLK++VRTFE+HCG+LSQYGMKHMRS AN+CN GI KE+M EASAQAC  IP  PWSSL +GFSA
Sbjct:  412 SALVDDWDCLKTMVRTFETHCGSLSQYGMKHMRSFANMCNVGIKKEQMREASAQACVTIPANPWSSLQRGFSA 484

HSP 2 Score: 75.8702 bits (185), Expect = 2.151e-43
Identity = 35/55 (63.64%), Postives = 43/55 (78.18%), Query Frame = -3 
Query:  541 APEGTPRKVEAQKQFFEAMSHGMHVDHSIKLIGKLLFGIEKGPEILNTVPTCWSA 705
            APEG+P+K EA+KQ  E MSH MH+D S++L+GKLLFGIEK PE+LN V    SA
Sbjct:  359 APEGSPKKNEARKQVLEVMSHRMHIDDSVELVGKLLFGIEKAPELLNAVRPAGSA 413
[Close]
BLAST of CN191807 vs. ExPASy Swiss-Prot
Match: VPEG_ARATH (Vacuolar-processing enzyme gamma-isozyme OS=Arabidopsis thaliana GN=At4g32940 PE=2 SV=2)

HSP 1 Score: 113.62 bits (283), Expect = 2.095e-40
Identity = 49/71 (69.01%), Postives = 59/71 (83.10%), Query Frame = -2 
Query:  326 IVDDWGCLKSLVRTFESHCGALSQYGMKHMRSLANICNTGIGKEKMAEASAQACENIPFGPWSSLDKGFSA 538
            +VDDW CLK+ VR FE HCG+LSQYG+KHMRS ANICN GI  E+M EA++QAC  +P GPWSSL++GFSA
Sbjct:  424 LVDDWNCLKNQVRAFERHCGSLSQYGIKHMRSFANICNAGIQMEQMEEAASQACTTLPTGPWSSLNRGFSA 494

HSP 2 Score: 73.559 bits (179), Expect = 2.095e-40
Identity = 32/49 (65.31%), Postives = 41/49 (83.67%), Query Frame = -3 
Query:  559 APEGTPRKVEAQKQFFEAMSHGMHVDHSIKLIGKLLFGIEKGPEILNTV 705
            APEG+ RK EAQKQ  EAMSH +H+D+S+ L+GK+LFGI +GPE+LN V
Sbjct:  369 APEGSARKTEAQKQVLEAMSHRLHIDNSVILVGKILFGISRGPEVLNKV 417
[Close]
BLAST of CN191807 vs. ExPASy Swiss-Prot
Match: VPE_VICSA (Vacuolar-processing enzyme OS=Vicia sativa PE=1 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 3.556e-40
Identity = 53/73 (72.60%), Postives = 61/73 (83.56%), Query Frame = -2 
Query:  326 SLIVDDWGCLKSLVRTFESHCGALSQYGMKHMRSLANICNTGIGKEKMAEASAQACENIPFGPWSSLDKGFSA 544
            S +VD+W CLK++V+TFE+HCG+LSQYGMKHMRS ANICN GI  E MAEASAQAC +IP  PWSSL  GFSA
Sbjct:  421 SPLVDNWDCLKTMVKTFETHCGSLSQYGMKHMRSFANICNAGIPNEPMAEASAQACASIPANPWSSLQGGFSA 493

HSP 2 Score: 69.3218 bits (168), Expect = 3.556e-40
Identity = 32/49 (65.31%), Postives = 41/49 (83.67%), Query Frame = -3 
Query:  559 APEGTPRKVEAQKQFFEAMSHGMHVDHSIKLIGKLLFGIEKGPEILNTV 705
            APEG+ +K EA+KQ  EAMSH  H+D+S+KLIG+LLFGIEKG E+L+ V
Sbjct:  368 APEGSSQKNEAEKQVLEAMSHRKHIDNSVKLIGQLLFGIEKGTELLDVV 416
[Close]
BLAST of CN191807 vs. ExPASy Swiss-Prot
Match: VPEA_ARATH (Vacuolar-processing enzyme alpha-isozyme OS=Arabidopsis thaliana GN=At2g25940 PE=2 SV=2)

HSP 1 Score: 114.005 bits (284), Expect = 9.199e-38
Identity = 50/71 (70.42%), Postives = 58/71 (81.69%), Query Frame = -2 
Query:  326 IVDDWGCLKSLVRTFESHCGALSQYGMKHMRSLANICNTGIGKEKMAEASAQACENIPFGPWSSLDKGFSA 538
            +VDDW CLKSLVR FE HCG+LSQYG+KHMRS+AN+CN GI   +M EA+ QAC  IP  PWSSLD+GFSA
Sbjct:  408 LVDDWDCLKSLVRAFERHCGSLSQYGIKHMRSIANMCNAGIQMRQMEEAAMQACPTIPTSPWSSLDRGFSA 478

HSP 2 Score: 64.3142 bits (155), Expect = 9.199e-38
Identity = 34/49 (69.39%), Postives = 39/49 (79.59%), Query Frame = -3 
Query:  559 APEGTPRKVEAQKQFFEAMSHGMHVDHSIKLIGKLLFGIEKGPEILNTV 705
            APEG+ RKVEAQKQ  EAMSH +HVD+SI LIG LLFG+E G  +LN V
Sbjct:  354 APEGSARKVEAQKQVLEAMSHRLHVDNSILLIGILLFGLE-GHAVLNKV 401
[Close]
BLAST of CN191807 vs. ExPASy Swiss-Prot
Match: VPE_RICCO (Vacuolar-processing enzyme OS=Ricinus communis PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.083e-24
Identity = 41/73 (56.16%), Postives = 51/73 (69.86%), Query Frame = -2 
Query:  326 SLIVDDWGCLKSLVRTFESHCGALSQYGMKHMRSLANICNTGIGKEKMAEASAQACENIPFGPWSSLDKGFSA 544
            S +VDDWGCLKS+VR FE+ CG+L+QYGMKHMR+ ANICN G+    M EA   AC     G W   ++G+SA
Sbjct:  425 SPLVDDWGCLKSMVRVFETCCGSLTQYGMKHMRTFANICNAGVSHTSMEEACNAACSGHDAGQWHPTNQGYSA 497

HSP 2 Score: 41.2022 bits (95), Expect = 1.083e-24
Identity = 20/46 (43.48%), Postives = 30/46 (65.22%), Query Frame = -3 
Query:  559 GTPRKVEAQKQFFEAMSHGMHVDHSIKLIGKLLFGIEKGPEILNTV 696
            G+ +K E  +Q  +A+ H  H+D S++LIG LLFG +K   IL +V
Sbjct:  375 GSEKKKEILQQIKDAIKHRSHLDSSMQLIGDLLFGPKKASAILKSV 420
[Close]
BLAST of CN191807 vs. ExPASy Swiss-Prot
Match: VPE_SOYBN (Vacuolar-processing enzyme OS=Glycine max PE=2 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 1.834e-24
Identity = 41/73 (56.16%), Postives = 53/73 (72.60%), Query Frame = -2 
Query:  326 SLIVDDWGCLKSLVRTFESHCGALSQYGMKHMRSLANICNTGIGKEKMAEASAQACENIPFGPWSSLDKGFSA 544
            S +VDDW CLKS+VR FE+HCG L+QYGMKHMR+ ANICN+G+ +  M EA   ACE    G +   ++G+SA
Sbjct:  423 SSLVDDWTCLKSMVRVFETHCGTLTQYGMKHMRAFANICNSGVSEASMEEACLAACEGYNAGLFHPSNRGYSA 495

HSP 2 Score: 40.0466 bits (92), Expect = 1.834e-24
Identity = 18/42 (42.86%), Postives = 26/42 (61.90%), Query Frame = -3 
Query:  559 KVEAQKQFFEAMSHGMHVDHSIKLIGKLLFGIEKGPEILNTV 684
            K +  KQ  E + H  H+D S++LIG LL+G  KG  +L +V
Sbjct:  377 KTDILKQIAETVKHRKHIDGSVELIGVLLYGPGKGSSVLQSV 418
[Close]
BLAST of CN191807 vs. ExPASy Swiss-Prot
Match: VPE2_PHAVU (Vacuolar-processing enzyme OS=Phaseolus vulgaris PE=2 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 2.098e-22
Identity = 37/71 (52.11%), Postives = 50/71 (70.42%), Query Frame = -2 
Query:  326 IVDDWGCLKSLVRTFESHCGALSQYGMKHMRSLANICNTGIGKEKMAEASAQACENIPFGPWSSLDKGFSA 538
            +VDDW CLKS+VR +E+HCG+L+QYGMKHMR+ ANICN+G+ +  M +A   AC     G     + G+SA
Sbjct:  423 LVDDWTCLKSMVRVYETHCGSLTQYGMKHMRAFANICNSGVSETSMEKACVAACGGYHAGLLHPSNTGYSA 493

HSP 2 Score: 40.817 bits (94), Expect = 2.098e-22
Identity = 19/45 (42.22%), Postives = 28/45 (62.22%), Query Frame = -3 
Query:  553 RKVEAQKQFFEAMSHGMHVDHSIKLIGKLLFGIEKGPEILNTVPT 687
            +K +  KQ  E + H  H+D S++LIG LL+G EK   +L +V T
Sbjct:  374 KKTDILKQIEEIVKHRKHLDGSVELIGVLLYGPEKASSVLRSVRT 418
[Close]
BLAST of CN191807 vs. ExPASy Swiss-Prot
Match: LEGU_CANEN (Legumain OS=Canavalia ensiformis PE=1 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 2.241e-21
Identity = 38/71 (53.52%), Postives = 49/71 (69.01%), Query Frame = -2 
Query:  326 IVDDWGCLKSLVRTFESHCGALSQYGMKHMRSLANICNTGIGKEKMAEASAQACENIPFGPWSSLDKGFSA 538
            +VDDW CLKS+VR FE+HCG+L+QYGMKHMR+  N+CN+G+ K  M EA   AC     G     + G+SA
Sbjct:  405 LVDDWTCLKSMVRVFETHCGSLTQYGMKHMRAFGNVCNSGVSKASMEEACKAACGGYDAGLLYPSNTGYSA 475

HSP 2 Score: 35.4242 bits (80), Expect = 2.241e-21
Identity = 16/43 (37.21%), Postives = 26/43 (60.47%), Query Frame = -3 
Query:  559 RKVEAQKQFFEAMSHGMHVDHSIKLIGKLLFGIEKGPEILNTV 687
            +K    +Q  E + H  H+D S++LIG LL+G  K   +L++V
Sbjct:  356 KKTHILEQITETVKHRNHLDGSVELIGVLLYGPGKSSSVLHSV 398
[Close]
BLAST of CN191807 vs. ExPASy Swiss-Prot
Match: VPEB_ARATH (Vacuolar-processing enzyme beta-isozyme OS=Arabidopsis thaliana GN=At1g62710 PE=2 SV=3)

HSP 1 Score: 89.7373 bits (221), Expect = 8.343e-21
Identity = 37/54 (68.52%), Postives = 44/54 (81.48%), Query Frame = -2 
Query:  377 IVDDWGCLKSLVRTFESHCGALSQYGMKHMRSLANICNTGIGKEKMAEASAQAC 538
            +VDDW CLKS+VR FE HCG+L+QYGMKHMR+ AN+CN G+ KE M EAS  AC
Sbjct:  414 LVDDWECLKSMVRVFEEHCGSLTQYGMKHMRAFANVCNNGVSKELMEEASTAAC 467

HSP 2 Score: 31.5722 bits (70), Expect = 8.343e-21
Identity = 13/36 (36.11%), Postives = 22/36 (61.11%), Query Frame = -3 
Query:  592 EGTPRKVEAQKQFFEAMSHGMHVDHSIKLIGKLLFG 699
            +G+ +K +  K+  E   H  H+D S++LI  +LFG
Sbjct:  363 DGSRKKDDTLKELTETTRHRKHLDASVELIATILFG 398
The following BLAST results are available for this feature:
BLAST of CN191807 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 10
Match NameE-valueIdentityDescription
VPE_CITSI3.054e-5897.18Vacuolar-processing enzyme OS=Citrus sinensis PE=2... [more]
VPE1_PHAVU2.151e-4373.97Vacuolar-processing enzyme OS=Phaseolus vulgaris P... [more]
VPEG_ARATH2.095e-4069.01Vacuolar-processing enzyme gamma-isozyme OS=Arabid... [more]
VPE_VICSA3.556e-4072.60Vacuolar-processing enzyme OS=Vicia sativa PE=1 SV... [more]
VPEA_ARATH9.199e-3870.42Vacuolar-processing enzyme alpha-isozyme OS=Arabid... [more]
VPE_RICCO1.083e-2456.16Vacuolar-processing enzyme OS=Ricinus communis PE=... [more]
VPE_SOYBN1.834e-2456.16Vacuolar-processing enzyme OS=Glycine max PE=2 SV=... [more]
VPE2_PHAVU2.098e-2252.11Vacuolar-processing enzyme OS=Phaseolus vulgaris P... [more]
LEGU_CANEN2.241e-2153.52Legumain OS=Canavalia ensiformis PE=1 SV=1[more]
VPEB_ARATH8.343e-2168.52Vacuolar-processing enzyme beta-isozyme OS=Arabido... [more]
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Properties
Property NameValue
Genbank descriptionUCRCS06_0005N23_f Washington Navel Orange Stored Fruit Rind cDNA Library Citrus sinensis cDNA clone CS_WEc0005N23, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN191807 ID=CN191807; Name=CN191807; organism=Citrus sinensis; type=EST; length=707bp
TTCGATATTGTACAGCAACACCACGTAAGAGGGTTGAGTTGCACGGTCTA
CTAATCAGGAACAAATATAGCTACGTAAGCATAAAATAGCATCGCATACA
TCCAAAATATATACTATCAAATTACAACTGCGGTCTGGCAGGGAAGAACT
TTTAAATATAATAATTACAAGGAAGCAATCACCAGCCAAATGAAAATAGA
CATTGCCATAAATGGAAGCAATCATCAGCCAAATGAAAATAGATATTGCC
ATATTGGCGACTATAATGAAAACTAACATATTTCAAGACTGGCTTATGAT
ATTCGCACAGCTCTCGGCTTCTTTATGCACTGAATCCTTTGTCGAGAGAA
CTCCAAGGACCAAAAGGAATATTTTCACAGGCTTGTGCTGATGCTTCAGC
CATCTTCTCTTTCCCAATTCCTGTATTACAGATGTTTGCAAGAGAACGCA
TGTGCTTCATTCCATATTGCGATAGAGCTCCACAGTGCGACTCAAAAGTC
CTCACCAGTGATTTGAGGCAGCCCCAGTCATCAACAATGAGGCTGACCAG
CAGGTCGGAACAGTGTTCAAGATCTCTGGACCTTTCTCAATTCCAAATAA
AAGCTTTCCAATAAGTTTGATGCTGTGGTCTACATGCATTCCATGAGACA
TTGCTTCAAAAAACTGCTTCTGAGCTTCCACCTTCCTAGGAGTGCCCTCA
GGTGCCT
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