EY715966 | Citrus Genome Database

Overview


Name	EY715966
Unique Name	EY715966
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	948

Libraries


Library Name	Type
Sweet orange fruit, development stadium (3 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
EY715966_ssr767	microsatellite	EY715966_ssr767:767..778.	BLAST: Citrus ESTs to Prunus persica proteins V1

Homology

[Close]

BLAST of EY715966 vs. ExPASy Swiss-Prot
Match: UBC1_ARATH (Ubiquitin-conjugating enzyme E2 1 OS=Arabidopsis thaliana GN=UBC1 PE=1 SV=1)

HSP 1 Score: 177.948 bits (450), Expect = 2.317e-48
Identity = 81/89 (91.01%), Postives = 86/89 (96.63%), Query Frame = 1

Query:   55 MSTPAKKRLMRDFKRLRRDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTGRFVIRMFHPYIYADGSICL 321
            MSTPA+KRLMRDFKRL++DPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKL+LQF+EDYPNKPPT RFV RMFHP IYADGSICL
Sbjct:    1 MSTPARKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLSLQFSEDYPNKPPTVRFVSRMFHPNIYADGSICL 89

HSP 2 Score: 35.8094 bits (81), Expect = 2.317e-48
Identity = 15/17 (88.24%), Postives = 16/17 (94.12%), Query Frame = 2

Query:  323 DILQNQWSPIFDVVAIL 373
            DILQNQWSPI+DV AIL
Sbjct:   90 DILQNQWSPIYDVAAIL 106

HSP 3 Score: 21.557 bits (44), Expect = 2.317e-48
Identity = 10/19 (52.63%), Postives = 13/19 (68.42%), Query Frame = 3

Query:  375 TCIRSLVWDPGEIFPAHSE 431
            T I+SL+ DP    PA+SE
Sbjct:  107 TSIQSLLCDPNPNSPANSE 125

[Close]

BLAST of EY715966 vs. ExPASy Swiss-Prot
Match: UBC2_MEDSA (Ubiquitin-conjugating enzyme E2 2 OS=Medicago sativa GN=UBC2 PE=2 SV=1)

HSP 1 Score: 177.563 bits (449), Expect = 3.014e-48
Identity = 81/89 (91.01%), Postives = 85/89 (95.51%), Query Frame = 1

Query:   55 MSTPAKKRLMRDFKRLRRDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTGRFVIRMFHPYIYADGSICL 321
            MSTPA+KRLMRDFKRL+ DPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKL+LQF+EDYPNKPPT RFV RMFHP IYADGSICL
Sbjct:    1 MSTPARKRLMRDFKRLQHDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLSLQFSEDYPNKPPTVRFVSRMFHPNIYADGSICL 89

HSP 2 Score: 35.8094 bits (81), Expect = 3.014e-48
Identity = 15/17 (88.24%), Postives = 16/17 (94.12%), Query Frame = 2

Query:  323 DILQNQWSPIFDVVAIL 373
            DILQNQWSPI+DV AIL
Sbjct:   90 DILQNQWSPIYDVAAIL 106

HSP 3 Score: 21.557 bits (44), Expect = 3.014e-48
Identity = 10/19 (52.63%), Postives = 13/19 (68.42%), Query Frame = 3

Query:  375 TCIRSLVWDPGEIFPAHSE 431
            T I+SL+ DP    PA+SE
Sbjct:  107 TSIQSLLCDPNPNSPANSE 125

[Close]

BLAST of EY715966 vs. ExPASy Swiss-Prot
Match: UBC3_ARATH (Ubiquitin-conjugating enzyme E2 3 OS=Arabidopsis thaliana GN=UBC3 PE=2 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 8.602e-45
Identity = 76/89 (85.39%), Postives = 81/89 (91.01%), Query Frame = 1

Query:   55 MSTPAKKRLMRDFKRLRRDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTGRFVIRMFHPYIYADGSICL 321
            M+TPAKKRLM DFKRL++DPP GISGAPQDNNIM WNA+IFGP+DTPWDGGTFKLTL FTEDYPNKPP  RFV RMFHP IYADGSICL
Sbjct:    1 MTTPAKKRLMWDFKRLQKDPPVGISGAPQDNNIMHWNALIFGPEDTPWDGGTFKLTLHFTEDYPNKPPIVRFVSRMFHPNIYADGSICL 89

HSP 2 Score: 35.4242 bits (80), Expect = 8.602e-45
Identity = 14/17 (82.35%), Postives = 16/17 (94.12%), Query Frame = 2

Query:  323 DILQNQWSPIFDVVAIL 373
            DILQNQWSPI+DV A+L
Sbjct:   90 DILQNQWSPIYDVAAVL 106

[Close]

BLAST of EY715966 vs. ExPASy Swiss-Prot
Match: UBC2_ARATH (Ubiquitin-conjugating enzyme E2 2 OS=Arabidopsis thaliana GN=UBC2 PE=2 SV=1)

HSP 1 Score: 178.718 bits (452), Expect = 4.242e-44
Identity = 89/148 (60.14%), Postives = 104/148 (70.27%), Query Frame = 1

Query:   55 MSTPAKKRLMRDFKRLRRDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTGRFVIRMFHPYIYADGSICLGYIAKSVESYI*CCSYTGPVFGRWCGIRVRFFLLTPKTTRVLLDAKRNNRPKSREILEQ 498
            MSTPA+KRLMRDFKRL++DPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKL+LQF+EDYPNKPPT RFV RMFHP IYADGSICL  +           +    +    C           +  R+  ++KR    + RE++EQ
Sbjct:    1 MSTPARKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLSLQFSEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPN-SPANSEAARMFSESKREYNRRVREVVEQ 147

[Close]

BLAST of EY715966 vs. ExPASy Swiss-Prot
Match: UBC2_WHEAT (Ubiquitin-conjugating enzyme E2 2 OS=Triticum aestivum GN=UBC2 PE=1 SV=1)

HSP 1 Score: 177.178 bits (448), Expect = 1.234e-43
Identity = 90/148 (60.81%), Postives = 101/148 (68.24%), Query Frame = 1

Query:   55 MSTPAKKRLMRDFKRLRRDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTGRFVIRMFHPYIYADGSICLGYIAKSVESYI*CCSYTGPVFGRWCGIRVRFFLLTPKTTRVLLDAKRNNRPKSREILEQ 498
            MSTPA+KRLMRDFKRL++DPPAGISGAP DNNI LWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPT RFV RMFHP IYADGSICL  +           +    +    C           +  R+  + KR    K RE++EQ
Sbjct:    1 MSTPARKRLMRDFKRLQQDPPAGISGAPHDNNITLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPN-SPANSEAARMYSENKREYNRKVREVVEQ 147

[Close]

BLAST of EY715966 vs. ExPASy Swiss-Prot
Match: UBE2A_MOUSE (Ubiquitin-conjugating enzyme E2 A OS=Mus musculus GN=Ube2a PE=2 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 2.624e-40
Identity = 68/89 (76.40%), Postives = 82/89 (92.13%), Query Frame = 1

Query:   55 MSTPAKKRLMRDFKRLRRDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTGRFVIRMFHPYIYADGSICL 321
            MSTPA++RLMRDFKRL+ DPPAG+SGAP +NNIM+WNAVIFGP+ TP++ GTFKLT++FTE+YPNKPPT RFV +MFHP +YADGSICL
Sbjct:    1 MSTPARRRLMRDFKRLQEDPPAGVSGAPSENNIMVWNAVIFGPEGTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKMFHPNVYADGSICL 89

HSP 2 Score: 30.4166 bits (67), Expect = 2.624e-40
Identity = 12/17 (70.59%), Postives = 15/17 (88.24%), Query Frame = 2

Query:  323 DILQNQWSPIFDVVAIL 373
            DILQN+WSP +DV +IL
Sbjct:   90 DILQNRWSPTYDVSSIL 106

[Close]

BLAST of EY715966 vs. ExPASy Swiss-Prot
Match: UBE2A_HUMAN (Ubiquitin-conjugating enzyme E2 A OS=Homo sapiens GN=UBE2A PE=2 SV=2)

HSP 1 Score: 157.147 bits (396), Expect = 2.624e-40
Identity = 68/89 (76.40%), Postives = 82/89 (92.13%), Query Frame = 1

Query:   55 MSTPAKKRLMRDFKRLRRDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTGRFVIRMFHPYIYADGSICL 321
            MSTPA++RLMRDFKRL+ DPPAG+SGAP +NNIM+WNAVIFGP+ TP++ GTFKLT++FTE+YPNKPPT RFV +MFHP +YADGSICL
Sbjct:    1 MSTPARRRLMRDFKRLQEDPPAGVSGAPSENNIMVWNAVIFGPEGTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKMFHPNVYADGSICL 89

HSP 2 Score: 30.4166 bits (67), Expect = 2.624e-40
Identity = 12/17 (70.59%), Postives = 15/17 (88.24%), Query Frame = 2

Query:  323 DILQNQWSPIFDVVAIL 373
            DILQN+WSP +DV +IL
Sbjct:   90 DILQNRWSPTYDVSSIL 106

[Close]

BLAST of EY715966 vs. ExPASy Swiss-Prot
Match: UBCD6_DROME (Ubiquitin-conjugating enzyme E2-17 kDa OS=Drosophila melanogaster GN=UbcD6 PE=2 SV=2)

HSP 1 Score: 155.606 bits (392), Expect = 3.418e-40
Identity = 67/89 (75.28%), Postives = 80/89 (89.89%), Query Frame = 1

Query:   55 MSTPAKKRLMRDFKRLRRDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTGRFVIRMFHPYIYADGSICL 321
            MSTPA++RLMRDFKRL+ DPP G+SGAP DNNIM+WNAVIFGP DTP++ GTFKLT++FTE+YPNKPPT RFV ++FHP +YADG ICL
Sbjct:    1 MSTPARRRLMRDFKRLQEDPPTGVSGAPTDNNIMIWNAVIFGPHDTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKVFHPNVYADGGICL 89

HSP 2 Score: 31.5722 bits (70), Expect = 3.418e-40
Identity = 13/17 (76.47%), Postives = 15/17 (88.24%), Query Frame = 2

Query:  323 DILQNQWSPIFDVVAIL 373
            DILQN+WSP +DV AIL
Sbjct:   90 DILQNRWSPTYDVSAIL 106

[Close]

BLAST of EY715966 vs. ExPASy Swiss-Prot
Match: UBE2B_RAT (Ubiquitin-conjugating enzyme E2 B OS=Rattus norvegicus GN=Ube2b PE=2 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 4.002e-38
Identity = 64/89 (71.91%), Postives = 79/89 (88.76%), Query Frame = 1

Query:   55 MSTPAKKRLMRDFKRLRRDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTGRFVIRMFHPYIYADGSICL 321
            MSTPA++RLMRDFKRL+ DPP G+SGAP +NNIM WNAVIFGP+ TP++ GTFKL ++F+E+YPNKPPT RF+ +MFHP +YADGSICL
Sbjct:    1 MSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICL 89

HSP 2 Score: 30.4166 bits (67), Expect = 4.002e-38
Identity = 12/17 (70.59%), Postives = 15/17 (88.24%), Query Frame = 2

Query:  323 DILQNQWSPIFDVVAIL 373
            DILQN+WSP +DV +IL
Sbjct:   90 DILQNRWSPTYDVSSIL 106

[Close]

BLAST of EY715966 vs. ExPASy Swiss-Prot
Match: UBE2B_RABIT (Ubiquitin-conjugating enzyme E2 B OS=Oryctolagus cuniculus GN=UBE2B PE=2 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 4.002e-38
Identity = 64/89 (71.91%), Postives = 79/89 (88.76%), Query Frame = 1

Query:   55 MSTPAKKRLMRDFKRLRRDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTGRFVIRMFHPYIYADGSICL 321
            MSTPA++RLMRDFKRL+ DPP G+SGAP +NNIM WNAVIFGP+ TP++ GTFKL ++F+E+YPNKPPT RF+ +MFHP +YADGSICL
Sbjct:    1 MSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICL 89

HSP 2 Score: 30.4166 bits (67), Expect = 4.002e-38
Identity = 12/17 (70.59%), Postives = 15/17 (88.24%), Query Frame = 2

Query:  323 DILQNQWSPIFDVVAIL 373
            DILQN+WSP +DV +IL
Sbjct:   90 DILQNRWSPTYDVSSIL 106

The following BLAST results are available for this feature:

BLAST of EY715966 vs. ExPASy Swiss-Prot

BLAST of EY715966 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 147


Match Name	E-value	Identity	Description
UBC1_ARATH	2.317e-48	91.01	Ubiquitin-conjugating enzyme E2 1 OS=Arabidopsis t...	[more]
UBC2_MEDSA	3.014e-48	91.01	Ubiquitin-conjugating enzyme E2 2 OS=Medicago sati...	[more]
UBC3_ARATH	8.602e-45	85.39	Ubiquitin-conjugating enzyme E2 3 OS=Arabidopsis t...	[more]
UBC2_ARATH	4.242e-44	60.14	Ubiquitin-conjugating enzyme E2 2 OS=Arabidopsis t...	[more]
UBC2_WHEAT	1.234e-43	60.81	Ubiquitin-conjugating enzyme E2 2 OS=Triticum aest...	[more]
UBE2A_MOUSE	2.624e-40	76.40	Ubiquitin-conjugating enzyme E2 A OS=Mus musculus ...	[more]
UBE2A_HUMAN	2.624e-40	76.40	Ubiquitin-conjugating enzyme E2 A OS=Homo sapiens ...	[more]
UBCD6_DROME	3.418e-40	75.28	Ubiquitin-conjugating enzyme E2-17 kDa OS=Drosophi...	[more]
UBE2B_RAT	4.002e-38	71.91	Ubiquitin-conjugating enzyme E2 B OS=Rattus norveg...	[more]
UBE2B_RABIT	4.002e-38	71.91	Ubiquitin-conjugating enzyme E2 B OS=Oryctolagus c...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C3-702-060-H01-CT.F Sweet orange fruit, development stadium (3 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY715966 ID=EY715966; Name=EY715966; organism=Citrus sinensis; type=EST; length=948bp
CTTTTCTCTAGAACTTTCCTCTCTCGTTCTCTCTCTGTAGAACACGACGT
CGCCATGTCAACACCGGCGAAGAAGCGGCTGATGCGGGACTTCAAGCGCC
TCCGGCGAGATCCACCCGCCGGCATCAGCGGTGCCCCTCAAGACAACAAC
ATCATGTTGTGGAACGCCGTTATTTTTGGGCCCGATGATACTCCCTGGGA
CGGAGGTACGTTCAAGTTGACTCTACAATTCACAGAGGATTATCCAAATA
AACCCCCAACAGGTCGTTTCGTCATCCGGATGTTTCATCCCTATATCTAT
GCAGATGGAAGTATTTGTTTGGGATATATTGCAAAATCAGTGGAGTCCTA
TATTTGATGTTGTAGCTATACTGGACCTGTATTCGGTCGCTGGTGTGGGA
TCCGGGTGAGATTTTTCCTGCTCACTCCGAAGACGACTCGGGTGTTGCTT
GATGCTAAGCGAAATAATAGACCAAAGAGTCGGGAAATTCTGGAACAAAA
ATAGATAAGCGGAGTATAAGGCAGAGACAGAAGGGGGGGTGCGCCGCGCA
GAGTAATGAGGAAATGGTCGGGGGGAGACTTTGTGTTACTTCAACGGGCG
GCCCTCACAACGGCCCGAGAGAGGGGGGGTGGGGAATTATGTTCGGTAGG
TGTGGGGGGTTCGGAGGGGGAGGGGCGCGGGGGGTCGCGAGACGCGGGAG
AAAACGAGAACAGGGGGGGAGGGGAGGAAGACGGGATAGAACATAGCGGG
GGGGGAAGTATAGGCCGGGAGGGAGGGAAGGATAAAGGGGAGGTGGGAGG
GGGAAGGGAGGATAGAAGAGGGGGAAGCGGAGAAGGAGAAGAAGGAGGAG
GGGAGGGGAGANAGGGGGGAGGGGATGAGTGGGACGGAGGGATGGAGGAG
GGAGGAGAGAGGGAGAGAGAGGGTGATGGAAGGAAAGCGAGGTGCCTG