EY679084 | Citrus Genome Database

Overview


Name	EY679084
Unique Name	EY679084
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	843

Libraries


Library Name	Type
Sweet orange leaf, infected with CiLV	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

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BLAST of EY679084 vs. ExPASy Swiss-Prot
Match: DNJH_CUCSA (DnaJ protein homolog OS=Cucumis sativus GN=DNAJ1 PE=2 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 2.529e-35
Identity = 40/41 (97.56%), Postives = 40/41 (97.56%), Query Frame = 1

Query:   25 MFGRAPKKSDNTKYYEILGVSKNASQDDLKKAYRKAAIKNH 147
            MFGR PKKSDNTKYYEILGVSKNASQDDLKKAYRKAAIKNH
Sbjct:    1 MFGR-PKKSDNTKYYEILGVSKNASQDDLKKAYRKAAIKNH 40

HSP 2 Score: 65.4698 bits (158), Expect = 2.529e-35
Identity = 39/72 (54.17%), Postives = 40/72 (55.56%), Query Frame = 2

Query:  188 AEAYEVLSDPEKREIYDQYGEDALKEXXXXXXXAHDPFDI-XXXXXXXXXXXXXXXXXXXXXXXEDVIHPLK 400
            A+AYEVLSDPEKREIYDQYGEDALKE        HDPFDI                        EDVIHPLK
Sbjct:   54 AQAYEVLSDPEKREIYDQYGEDALKE-GMGGGGGHDPFDIFQSFFGGSPFGGGGSSRGRRQRRGEDVIHPLK 124

HSP 3 Score: 45.4394 bits (106), Expect = 2.529e-35
Identity = 25/44 (56.82%), Postives = 30/44 (68.18%), Query Frame = 3

Query:  393 PSRVGGGEGYNGTSKKLSLSRNVICTRWQRKGV*IRGHQMKCSG 524
            P +V   + YNGTSKKLSLSRNVIC++ + KG    G  MKC G
Sbjct:  122 PLKVSLEDLYNGTSKKLSLSRNVICSKCKGKGS-KSGASMKCPG 164

[Close]

BLAST of EY679084 vs. ExPASy Swiss-Prot
Match: DNJH2_ALLPO (DnaJ protein homolog 2 OS=Allium porrum GN=LDJ2 PE=2 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 3.469e-34
Identity = 37/41 (90.24%), Postives = 40/41 (97.56%), Query Frame = 1

Query:   25 MFGRAPKKSDNTKYYEILGVSKNASQDDLKKAYRKAAIKNH 147
            MFGRAPKKSDNTKYYE+LGVSKNA+ +DLKKAYRKAAIKNH
Sbjct:    1 MFGRAPKKSDNTKYYEVLGVSKNATPEDLKKAYRKAAIKNH 41

HSP 2 Score: 63.5438 bits (153), Expect = 3.469e-34
Identity = 34/71 (47.89%), Postives = 38/71 (53.52%), Query Frame = 2

Query:  191 EAYEVLSDPEKREIYDQYGEDALKEXXXXXXXAHDPFDI-XXXXXXXXXXXXXXXXXXXXXXXEDVIHPLK 400
            +AYEVL+DPEKREIYDQYGE+ LKE        HDPFDI                        EDV+HPLK
Sbjct:   56 QAYEVLNDPEKREIYDQYGEEGLKEGMGGGGGVHDPFDIFQSFFGGGGFGGGGSSRGRRQRRGEDVVHPLK 126

HSP 3 Score: 42.743 bits (99), Expect = 3.469e-34
Identity = 23/43 (53.49%), Postives = 29/43 (67.44%), Query Frame = 3

Query:  393 PSRVGGGEGYNGTSKKLSLSRNVICTRWQRKGV*IRGHQMKCS 521
            P +V   + YNGTSKKLSLSRNV+CT+ + KG    G  M C+
Sbjct:  124 PLKVSLEDLYNGTSKKLSLSRNVLCTKCKGKGS-KSGASMNCA 165

[Close]

BLAST of EY679084 vs. ExPASy Swiss-Prot
Match: DNJH_ATRNU (DnaJ protein homolog ANJ1 OS=Atriplex nummularia PE=2 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 2.167e-33
Identity = 34/41 (82.93%), Postives = 39/41 (95.12%), Query Frame = 1

Query:   25 MFGRAPKKSDNTKYYEILGVSKNASQDDLKKAYRKAAIKNH 147
            MFGRAPKKSD+T+YYEILGV K+AS +DLKKAY+KAAIKNH
Sbjct:    1 MFGRAPKKSDSTRYYEILGVPKDASPEDLKKAYKKAAIKNH 41

HSP 2 Score: 68.1662 bits (165), Expect = 2.167e-33
Identity = 38/72 (52.78%), Postives = 39/72 (54.17%), Query Frame = 2

Query:  188 AEAYEVLSDPEKREIYDQYGEDALKEXXXXXXXAHDPFDI-XXXXXXXXXXXXXXXXXXXXXXXEDVIHPLK 400
            A AYEVLSDPEKREIYDQYGEDALKE        HDPFDI                        EDV+HPLK
Sbjct:   55 AHAYEVLSDPEKREIYDQYGEDALKEGMGGGGGMHDPFDIFQSFFGGSPFGGVGSSRGRRQRRGEDVVHPLK 126

HSP 3 Score: 41.9726 bits (97), Expect = 2.167e-33
Identity = 30/72 (41.67%), Postives = 41/72 (56.94%), Query Frame = 3

Query:  393 PSRVGGGEGYNGTSKKLSLSRNVICTRWQRKGV*IRGHQMKCSGLARKLEGKV*KKNRGPPQGIHKMHASCD 608
            P +V   + + GT+KKLSLSRNVIC++   KG    G  MKCSG  +    KV  ++ GP   I +M   C+
Sbjct:  124 PLKVSLEDLFTGTTKKLSLSRNVICSKCTGKGS-KSGASMKCSG-CQGTGMKVSIRHLGPSM-IQQMQHPCN 192

[Close]

BLAST of EY679084 vs. ExPASy Swiss-Prot
Match: DNAJ3_ARATH (Chaperone protein dnaJ 3 OS=Arabidopsis thaliana GN=ATJ3 PE=1 SV=2)

HSP 1 Score: 73.9442 bits (180), Expect = 2.965e-32
Identity = 38/71 (53.52%), Postives = 40/71 (56.34%), Query Frame = 2

Query:  188 AEAYEVLSDPEKREIYDQYGEDALKEXXXXXXXAHDPFDIXXXXXXXXXXXXXXXXXXXXXXXEDVIHPLK 400
            A+AYEVLSDPEKREIYDQYGEDALKE        HDPFDI                       EDV+HPLK
Sbjct:   56 AQAYEVLSDPEKREIYDQYGEDALKEGMGGGGGGHDPFDIFSSFFGGGPFGGNTSRQRRQRRGEDVVHPLK 126

HSP 2 Score: 69.707 bits (169), Expect = 2.965e-32
Identity = 34/42 (80.95%), Postives = 38/42 (90.48%), Query Frame = 1

Query:   25 MFGRAP-KKSDNTKYYEILGVSKNASQDDLKKAYRKAAIKNH 147
            MFGR P KKSDNTK+YEILGV K+AS +DLKKAY+KAAIKNH
Sbjct:    1 MFGRGPSKKSDNTKFYEILGVPKSASPEDLKKAYKKAAIKNH 42

HSP 3 Score: 36.965 bits (84), Expect = 2.965e-32
Identity = 20/44 (45.45%), Postives = 26/44 (59.09%), Query Frame = 3

Query:  393 PSRVGGGEGYNGTSKKLSLSRNVICTRWQRKGV*IRGHQMKCSG 524
            P +V   + Y GT KKLSLSRN +C++   KG    G  +KC G
Sbjct:  124 PLKVSLEDVYLGTMKKLSLSRNALCSKCNGKGS-KSGASLKCGG 166

[Close]

BLAST of EY679084 vs. ExPASy Swiss-Prot
Match: DNAJ2_ARATH (Chaperone protein dnaJ 2 OS=Arabidopsis thaliana GN=ATJ2 PE=1 SV=2)

HSP 1 Score: 69.3218 bits (168), Expect = 5.527e-30
Identity = 38/72 (52.78%), Postives = 40/72 (55.56%), Query Frame = 2

Query:  188 AEAYEVLSDPEKREIYDQYGEDALKEXXXXXXXAHDPFDI-XXXXXXXXXXXXXXXXXXXXXXXEDVIHPLK 400
            A+AYEVLSDPEKREIYDQYGEDALKE        HDPFDI                        EDV+HPLK
Sbjct:   56 AQAYEVLSDPEKREIYDQYGEDALKEGMGGGGGGHDPFDIFSSFFGSGGHPFGSHSRGRRQRRGEDVVHPLK 127

HSP 2 Score: 67.0106 bits (162), Expect = 5.527e-30
Identity = 32/42 (76.19%), Postives = 37/42 (88.10%), Query Frame = 1

Query:   25 MFGRAP-KKSDNTKYYEILGVSKNASQDDLKKAYRKAAIKNH 147
            MFGR P +KSDNTK+YEILGV K A+ +DLKKAY+KAAIKNH
Sbjct:    1 MFGRGPSRKSDNTKFYEILGVPKTAAPEDLKKAYKKAAIKNH 42

HSP 3 Score: 36.5798 bits (83), Expect = 5.527e-30
Identity = 20/44 (45.45%), Postives = 26/44 (59.09%), Query Frame = 3

Query:  393 PSRVGGGEGYNGTSKKLSLSRNVICTRWQRKGV*IRGHQMKCSG 524
            P +V   + Y GT+KKLSLSR  +C++   KG    G  MKC G
Sbjct:  125 PLKVSLEDVYLGTTKKLSLSRKALCSKCNGKGS-KSGASMKCGG 167

[Close]

BLAST of EY679084 vs. ExPASy Swiss-Prot
Match: DNJH1_ALLPO (DnaJ protein homolog 1 (Fragment) OS=Allium porrum GN=DNAJ1 PE=2 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 5.561e-24
Identity = 37/72 (51.39%), Postives = 40/72 (55.56%), Query Frame = 2

Query:  188 AEAYEVLSDPEKREIYDQYGEDALKEXXXXXXXAHDPFDI-XXXXXXXXXXXXXXXXXXXXXXXEDVIHPLK 400
            A+AY+VLSDPEKREIYDQYGEDALKE        HDPFDI                        EDV+HPLK
Sbjct:   34 AQAYDVLSDPEKREIYDQYGEDALKEGMGGGGGDHDPFDIFQSFFGGGGFGGGGSSRGRRQRRGEDVVHPLK 105

HSP 2 Score: 43.5134 bits (101), Expect = 5.561e-24
Identity = 24/43 (55.81%), Postives = 29/43 (67.44%), Query Frame = 3

Query:  393 PSRVGGGEGYNGTSKKLSLSRNVICTRWQRKGV*IRGHQMKCS 521
            P +V   E YNGTSKKLSLSRNVIC++   KG    G  M+C+
Sbjct:  103 PLKVSLEELYNGTSKKLSLSRNVICSKCNGKGS-KSGASMRCA 144

HSP 3 Score: 41.5874 bits (96), Expect = 5.561e-24
Identity = 19/20 (95.00%), Postives = 19/20 (95.00%), Query Frame = 1

Query:   88 KNASQDDLKKAYRKAAIKNH 147
            KNAS DDLKKAYRKAAIKNH
Sbjct:    1 KNASPDDLKKAYRKAAIKNH 20

[Close]

BLAST of EY679084 vs. ExPASy Swiss-Prot
Match: DNJA4_HUMAN (DnaJ homolog subfamily A member 4 OS=Homo sapiens GN=DNAJA4 PE=1 SV=1)

HSP 1 Score: 46.595 bits (109), Expect = 1.562e-14
Identity = 21/40 (52.50%), Postives = 27/40 (67.50%), Query Frame = 2

Query:  188 AEAYEVLSDPEKREIYDQYGEDALKEXXXXXXXAHDPFDI 307
            ++AYEVLSDP+KR++YDQ GE A+KE          P DI
Sbjct:   49 SQAYEVLSDPKKRDVYDQGGEQAIKEGGSGSPSFSSPMDI 88

HSP 2 Score: 43.1282 bits (100), Expect = 1.562e-14
Identity = 18/30 (60.00%), Postives = 25/30 (83.33%), Query Frame = 1

Query:   58 TKYYEILGVSKNASQDDLKKAYRKAAIKNH 147
            T+YY+ILGV  +AS +++KKAYRK A+K H
Sbjct:    5 TQYYDILGVKPSASPEEIKKAYRKLALKYH 34

HSP 3 Score: 30.4166 bits (67), Expect = 1.562e-14
Identity = 12/23 (52.17%), Postives = 17/23 (73.91%), Query Frame = 3

Query:  420 YNGTSKKLSLSRNVICTRWQRKG 488
            YNG +KKL+L +NVIC + +  G
Sbjct:  120 YNGVTKKLALQKNVICEKCEGVG 142

[Close]

BLAST of EY679084 vs. ExPASy Swiss-Prot
Match: DNJA4_MOUSE (DnaJ homolog subfamily A member 4 OS=Mus musculus GN=Dnaja4 PE=2 SV=1)

HSP 1 Score: 46.9802 bits (110), Expect = 2.020e-14
Identity = 22/40 (55.00%), Postives = 27/40 (67.50%), Query Frame = 2

Query:  188 AEAYEVLSDPEKREIYDQYGEDALKEXXXXXXXAHDPFDI 307
            ++AYEVLSDP+KR+IYDQ GE A+KE          P DI
Sbjct:   49 SQAYEVLSDPKKRDIYDQGGEQAIKEGGSGSPSFSSPMDI 88

HSP 2 Score: 43.1282 bits (100), Expect = 2.020e-14
Identity = 18/30 (60.00%), Postives = 25/30 (83.33%), Query Frame = 1

Query:   58 TKYYEILGVSKNASQDDLKKAYRKAAIKNH 147
            T+YY+ILGV  +AS +++KKAYRK A+K H
Sbjct:    5 TQYYDILGVKPSASPEEIKKAYRKLALKYH 34

HSP 3 Score: 29.6462 bits (65), Expect = 2.020e-14
Identity = 12/23 (52.17%), Postives = 17/23 (73.91%), Query Frame = 3

Query:  420 YNGTSKKLSLSRNVICTRWQRKG 488
            YNG +KKL+L +NVIC + +  G
Sbjct:  120 YNGITKKLALQKNVICEKCEGIG 142

[Close]

BLAST of EY679084 vs. ExPASy Swiss-Prot
Match: DNJA2_RAT (DnaJ homolog subfamily A member 2 OS=Rattus norvegicus GN=Dnaja2 PE=2 SV=1)

HSP 1 Score: 47.7506 bits (112), Expect = 1.221e-13
Identity = 26/69 (37.68%), Postives = 32/69 (46.38%), Query Frame = 2

Query:  194 AYEVLSDPEKREIYDQYGEDALKEXXXXXXXAHDPFDIXXXXXXXXXXXXXXXXXXXXXXXEDVIHPLK 400
            AYEVLS+PEKRE+YD+YGE  L+E         D F                         ED++HPLK
Sbjct:   53 AYEVLSNPEKRELYDRYGEQGLREGSGGGGGMDDIFSHIFGGGLFGFMGNQSRSRNGRRRGEDMMHPLK 121

HSP 2 Score: 39.2762 bits (90), Expect = 1.221e-13
Identity = 18/31 (58.06%), Postives = 24/31 (77.42%), Query Frame = 1

Query:   55 NTKYYEILGVSKNASQDDLKKAYRKAAIKNH 147
            +TK Y+ILGV   AS+++LKKAYRK A + H
Sbjct:    6 DTKLYDILGVPPGASENELKKAYRKLAKEYH 36

HSP 3 Score: 30.0314 bits (66), Expect = 1.221e-13
Identity = 22/74 (29.73%), Postives = 36/74 (48.65%), Query Frame = 3

Query:  393 PSRVGGGEGYNGTSKKLSLSRNVICTRWQRKGV*IRGHQMKCSGL-ARKLEGKV*KKNRGPPQGIHKMHASCDG 611
            P +V   + YNG + KL LS+NV+C+    +G    G   KCS    R +   + +   G  Q +  + + C+G
Sbjct:  119 PLKVSLEDLYNGKTTKLQLSKNVLCSACSGQGG-KSGAVQKCSACRGRGVRIMIRQLAPGMVQQMQSVCSDCNG 191

[Close]

BLAST of EY679084 vs. ExPASy Swiss-Prot
Match: DNJA2_MOUSE (DnaJ homolog subfamily A member 2 OS=Mus musculus GN=Dnaja2 PE=1 SV=1)

HSP 1 Score: 47.7506 bits (112), Expect = 1.221e-13
Identity = 26/69 (37.68%), Postives = 32/69 (46.38%), Query Frame = 2

Query:  194 AYEVLSDPEKREIYDQYGEDALKEXXXXXXXAHDPFDIXXXXXXXXXXXXXXXXXXXXXXXEDVIHPLK 400
            AYEVLS+PEKRE+YD+YGE  L+E         D F                         ED++HPLK
Sbjct:   53 AYEVLSNPEKRELYDRYGEQGLREGSGGGGGMDDIFSHIFGGGLFGFMGNQSRSRNGRRRGEDMMHPLK 121

HSP 2 Score: 39.2762 bits (90), Expect = 1.221e-13
Identity = 18/31 (58.06%), Postives = 24/31 (77.42%), Query Frame = 1

Query:   55 NTKYYEILGVSKNASQDDLKKAYRKAAIKNH 147
            +TK Y+ILGV   AS+++LKKAYRK A + H
Sbjct:    6 DTKLYDILGVPPGASENELKKAYRKLAKEYH 36

HSP 3 Score: 30.0314 bits (66), Expect = 1.221e-13
Identity = 22/74 (29.73%), Postives = 36/74 (48.65%), Query Frame = 3

Query:  393 PSRVGGGEGYNGTSKKLSLSRNVICTRWQRKGV*IRGHQMKCSGL-ARKLEGKV*KKNRGPPQGIHKMHASCDG 611
            P +V   + YNG + KL LS+NV+C+    +G    G   KCS    R +   + +   G  Q +  + + C+G
Sbjct:  119 PLKVSLEDLYNGKTTKLQLSKNVLCSACSGQGG-KSGAVQKCSACRGRGVRIMIRQLAPGMVQQMQSVCSDCNG 191

The following BLAST results are available for this feature:

BLAST of EY679084 vs. ExPASy Swiss-Prot

BLAST of EY679084 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 18


Match Name	E-value	Identity	Description
DNJH_CUCSA	2.529e-35	97.56	DnaJ protein homolog OS=Cucumis sativus GN=DNAJ1 P...	[more]
DNJH2_ALLPO	3.469e-34	90.24	DnaJ protein homolog 2 OS=Allium porrum GN=LDJ2 PE...	[more]
DNJH_ATRNU	2.167e-33	82.93	DnaJ protein homolog ANJ1 OS=Atriplex nummularia P...	[more]
DNAJ3_ARATH	2.965e-32	53.52	Chaperone protein dnaJ 3 OS=Arabidopsis thaliana G...	[more]
DNAJ2_ARATH	5.527e-30	52.78	Chaperone protein dnaJ 2 OS=Arabidopsis thaliana G...	[more]
DNJH1_ALLPO	5.561e-24	51.39	DnaJ protein homolog 1 (Fragment) OS=Allium porrum...	[more]
DNJA4_HUMAN	1.562e-14	52.50	DnaJ homolog subfamily A member 4 OS=Homo sapiens ...	[more]
DNJA4_MOUSE	2.020e-14	55.00	DnaJ homolog subfamily A member 4 OS=Mus musculus ...	[more]
DNJA2_RAT	1.221e-13	37.68	DnaJ homolog subfamily A member 2 OS=Rattus norveg...	[more]
DNJA2_MOUSE	1.221e-13	37.68	DnaJ homolog subfamily A member 2 OS=Mus musculus ...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-401-055-A07-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY679084 ID=EY679084; Name=EY679084; organism=Citrus sinensis; type=EST; length=843bp
AGATCTGAAATTTTTTAGTACAAGATGTTCGGAAGAGCGCCAAAGAAAAG
CGATAACACAAAGTACTATGAGATTCTCGGAGTCTCAAAAAACGCTTCGC
AAGATGATCTGAAGAAGGCTTATAGGAAAGCCGCCATCAAAAACCATCCT
GACAAGGGCGGTGATCCTGAGAAGGGTGGGGGGGGGGGCGGAAGCGTATG
AGGTTCTGAGTGACCCAGAGAAGCGTGAAATATATGATCAATACGGCGAG
GATGCGCTTAAAGAGGGAATGGGTGGTGGTGGTGGTGCCCATGACCCATT
CGACATATTCCAATCCTTCTTCGGTGGCAGCCCATTTGGTGGTGGGAGCA
GCAAAGGAAGAAGGCAAAGGAGGGGAGAAGATGTAATCCATCCCCTCAAG
GGTGGGGGGGGGGGAGGGCTACAATGGGACATCTAAGAAGCTCTCGCTCT
CCCGCAATGTAATCTGCACCAGGTGGCAAAGGAAAGGGGTCTAAATCCGG
GGGCATCAAATGAAGTGTTCTGGGCTGGCCAGGAAACTGGAAGGAAAGGT
TTAAAAAAAGAACCGTGGGCCCCCTCAAGGGATCCACAAAATGCACGCAT
CGTGTGATGGGGGTGCAGGGGGTAGGGGGAAAAATATTCAAGGAGAATGA
TTGCCTGGTCCCTGAGGTGAGGAAGAAAAAGGCGATTGAGAGGAAAAAGA
AGTTTGGTAGGATATTGGTAGGTATATGGGATAATGGATTGTGCTGTAGA
ATAATAGGGTATATGTGGGAATAGTGGAATGATANGCGCCTCATCATTGG
TTGCGCGTCATAGCTTGGAAGGGTAGTTCGTTCGTCAGTGAGG