DR911208

Overview
NameDR911208
Unique NameDR911208
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length326
Libraries
Library NameType
Citrus sinensis phloemcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of DR911208 vs. ExPASy Swiss-Prot
Match: TPIS_PETHY (Triosephosphate isomerase, cytosolic OS=Petunia hybrida GN=TPIP1 PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 1.341e-19
Identity = 29/35 (82.86%), Postives = 31/35 (88.57%), Query Frame = 3 
Query:   75 KRLAAQPDVDGFLVGGASLKPEFIDIIKSATVKKN 179
            K L  QPDVDGFLVGGASLKPEFIDIIK+A VK+N
Sbjct:  219 KELGGQPDVDGFLVGGASLKPEFIDIIKAAEVKRN 253

HSP 2 Score: 53.9138 bits (128), Expect = 1.341e-19
Identity = 25/27 (92.59%), Postives = 26/27 (96.30%), Query Frame = 2 
Query:    5 NVSAEVAASTRIIYGGSVNGANCKEIG 85
            NVS EVAASTRIIYGGSVNGANCKE+G
Sbjct:  196 NVSPEVAASTRIIYGGSVNGANCKELG 222
[Close]
BLAST of DR911208 vs. ExPASy Swiss-Prot
Match: TPIS_LACSA (Triosephosphate isomerase, cytosolic (Fragment) OS=Lactuca sativa PE=2 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 6.533e-19
Identity = 28/35 (80.00%), Postives = 30/35 (85.71%), Query Frame = 3 
Query:   75 KRLAAQPDVDGFLVGGASLKPEFIDIIKSATVKKN 179
            K L  Q DVDGFLVGGASLKPEFIDIIK+A VKK+
Sbjct:  160 KELGGQTDVDGFLVGGASLKPEFIDIIKAAEVKKS 194

HSP 2 Score: 55.4546 bits (132), Expect = 6.533e-19
Identity = 25/31 (80.65%), Postives = 29/31 (93.55%), Query Frame = 2 
Query:    2 DNVSAEVAASTRIIYGGSVNGANCKEIGSTT 94
            DNVSAEV+ASTRIIYGGSV+G+NCKE+G  T
Sbjct:  136 DNVSAEVSASTRIIYGGSVSGSNCKELGGQT 166
[Close]
BLAST of DR911208 vs. ExPASy Swiss-Prot
Match: TPIS_ORYSJ (Triosephosphate isomerase, cytosolic OS=Oryza sativa subsp. japonica GN=TPI PE=1 SV=3)

HSP 1 Score: 61.6178 bits (148), Expect = 2.414e-18
Identity = 30/33 (90.91%), Postives = 31/33 (93.94%), Query Frame = 3 
Query:   75 KRLAAQPDVDGFLVGGASLKPEFIDIIKSATVK 173
            K LAA+PDVDGFLVGGASLKPEFIDII SATVK
Sbjct:  219 KELAAKPDVDGFLVGGASLKPEFIDIINSATVK 251

HSP 2 Score: 49.6766 bits (117), Expect = 2.414e-18
Identity = 23/28 (82.14%), Postives = 25/28 (89.29%), Query Frame = 2 
Query:    5 NVSAEVAASTRIIYGGSVNGANCKEIGS 88
            NVSAEVA STRIIYGGSV GANCKE+ +
Sbjct:  196 NVSAEVAESTRIIYGGSVTGANCKELAA 223
[Close]
BLAST of DR911208 vs. ExPASy Swiss-Prot
Match: TPIS_ARATH (Triosephosphate isomerase, cytosolic OS=Arabidopsis thaliana GN=CTIMC PE=1 SV=2)

HSP 1 Score: 57.7658 bits (138), Expect = 6.900e-18
Identity = 28/35 (80.00%), Postives = 30/35 (85.71%), Query Frame = 3 
Query:   75 KRLAAQPDVDGFLVGGASLKPEFIDIIKSATVKKN 179
            K L  Q DVDGFLVGGASLKPEFIDIIK+A VKK+
Sbjct:  219 KELGGQADVDGFLVGGASLKPEFIDIIKAAEVKKS 253

HSP 2 Score: 51.9878 bits (123), Expect = 6.900e-18
Identity = 23/27 (85.19%), Postives = 26/27 (96.30%), Query Frame = 2 
Query:    5 NVSAEVAASTRIIYGGSVNGANCKEIG 85
            NVSA+VAA+TRIIYGGSVNG NCKE+G
Sbjct:  196 NVSADVAATTRIIYGGSVNGGNCKELG 222
[Close]
BLAST of DR911208 vs. ExPASy Swiss-Prot
Match: TPIS_COPJA (Triosephosphate isomerase, cytosolic OS=Coptis japonica PE=2 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 2.564e-17
Identity = 29/36 (80.56%), Postives = 33/36 (91.67%), Query Frame = 3 
Query:   72 AKRLAAQPDVDGFLVGGASLKPEFIDIIKSATVKKN 179
            +K LA QPD+DGFLVGGASLKPEF+DIIKSATVK +
Sbjct:  218 SKELAGQPDIDGFLVGGASLKPEFVDIIKSATVKSS 253

HSP 2 Score: 44.2838 bits (103), Expect = 2.564e-17
Identity = 20/27 (74.07%), Postives = 23/27 (85.19%), Query Frame = 2 
Query:    2 DNVSAEVAASTRIIYGGSVNGANCKEI 82
            +NV A+VA S RIIYGGSVNGAN KE+
Sbjct:  195 ENVGADVAGSVRIIYGGSVNGANSKEL 221
[Close]
BLAST of DR911208 vs. ExPASy Swiss-Prot
Match: TPIS_SECCE (Triosephosphate isomerase, cytosolic OS=Secale cereale PE=2 SV=3)

HSP 1 Score: 62.003 bits (149), Expect = 2.564e-17
Identity = 30/33 (90.91%), Postives = 31/33 (93.94%), Query Frame = 3 
Query:   75 KRLAAQPDVDGFLVGGASLKPEFIDIIKSATVK 173
            K LAAQPDVDGFLVGGASLKPEFIDII +ATVK
Sbjct:  219 KELAAQPDVDGFLVGGASLKPEFIDIINAATVK 251

HSP 2 Score: 45.8246 bits (107), Expect = 2.564e-17
Identity = 21/28 (75.00%), Postives = 24/28 (85.71%), Query Frame = 2 
Query:    5 NVSAEVAASTRIIYGGSVNGANCKEIGS 88
            NVS EVA STRIIYGGSV GA+CKE+ +
Sbjct:  196 NVSPEVAESTRIIYGGSVTGASCKELAA 223
[Close]
BLAST of DR911208 vs. ExPASy Swiss-Prot
Match: TPIS_MAIZE (Triosephosphate isomerase, cytosolic OS=Zea mays PE=3 SV=3)

HSP 1 Score: 62.003 bits (149), Expect = 9.523e-17
Identity = 30/33 (90.91%), Postives = 31/33 (93.94%), Query Frame = 3 
Query:   75 KRLAAQPDVDGFLVGGASLKPEFIDIIKSATVK 173
            K LAAQPDVDGFLVGGASLKPEFIDII +ATVK
Sbjct:  219 KELAAQPDVDGFLVGGASLKPEFIDIINAATVK 251

HSP 2 Score: 43.8986 bits (102), Expect = 9.523e-17
Identity = 20/28 (71.43%), Postives = 22/28 (78.57%), Query Frame = 2 
Query:    5 NVSAEVAASTRIIYGGSVNGANCKEIGS 88
            N S EVA STRIIYGGSV  ANCKE+ +
Sbjct:  196 NASPEVAESTRIIYGGSVTAANCKELAA 223
[Close]
BLAST of DR911208 vs. ExPASy Swiss-Prot
Match: TPIS_HORVU (Triosephosphate isomerase, cytosolic OS=Hordeum vulgare PE=1 SV=3)

HSP 1 Score: 56.9954 bits (136), Expect = 7.761e-16
Identity = 28/33 (84.85%), Postives = 29/33 (87.88%), Query Frame = 3 
Query:   75 KRLAAQPDVDGFLVGGASLKPEFIDIIKSATVK 173
            K LAAQ DVDGFLVGGASLKPEFIDII +A VK
Sbjct:  219 KELAAQADVDGFLVGGASLKPEFIDIINAAAVK 251

HSP 2 Score: 45.8246 bits (107), Expect = 7.761e-16
Identity = 21/28 (75.00%), Postives = 24/28 (85.71%), Query Frame = 2 
Query:    5 NVSAEVAASTRIIYGGSVNGANCKEIGS 88
            NVS EVA STRIIYGGSV GA+CKE+ +
Sbjct:  196 NVSPEVAESTRIIYGGSVTGASCKELAA 223
[Close]
BLAST of DR911208 vs. ExPASy Swiss-Prot
Match: TPIS_MACMU (Triosephosphate isomerase OS=Macaca mulatta GN=TPI1 PE=3 SV=2)

HSP 1 Score: 53.5286 bits (127), Expect = 2.341e-14
Identity = 24/29 (82.76%), Postives = 27/29 (93.10%), Query Frame = 3 
Query:   75 KRLAAQPDVDGFLVGGASLKPEFIDIIKS 161
            K LA+QPDVDGFLVGGASLKPEF+DII +
Sbjct:  219 KELASQPDVDGFLVGGASLKPEFVDIINA 247

HSP 2 Score: 44.2838 bits (103), Expect = 2.341e-14
Identity = 21/28 (75.00%), Postives = 22/28 (78.57%), Query Frame = 2 
Query:    5 NVSAEVAASTRIIYGGSVNGANCKEIGS 88
            NVS  VA STRIIYGGSV GA CKE+ S
Sbjct:  196 NVSEAVAQSTRIIYGGSVTGATCKELAS 223
[Close]
BLAST of DR911208 vs. ExPASy Swiss-Prot
Match: TPIS_MACFA (Triosephosphate isomerase OS=Macaca fascicularis GN=TPI1 PE=2 SV=3)

HSP 1 Score: 53.5286 bits (127), Expect = 2.341e-14
Identity = 24/29 (82.76%), Postives = 27/29 (93.10%), Query Frame = 3 
Query:   75 KRLAAQPDVDGFLVGGASLKPEFIDIIKS 161
            K LA+QPDVDGFLVGGASLKPEF+DII +
Sbjct:  219 KELASQPDVDGFLVGGASLKPEFVDIINA 247

HSP 2 Score: 44.2838 bits (103), Expect = 2.341e-14
Identity = 21/28 (75.00%), Postives = 22/28 (78.57%), Query Frame = 2 
Query:    5 NVSAEVAASTRIIYGGSVNGANCKEIGS 88
            NVS  VA STRIIYGGSV GA CKE+ S
Sbjct:  196 NVSEAVAQSTRIIYGGSVTGATCKELAS 223
The following BLAST results are available for this feature:
BLAST of DR911208 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 35
Match NameE-valueIdentityDescription
TPIS_PETHY1.341e-1982.86Triosephosphate isomerase, cytosolic OS=Petunia hy... [more]
TPIS_LACSA6.533e-1980.00Triosephosphate isomerase, cytosolic (Fragment) OS... [more]
TPIS_ORYSJ2.414e-1890.91Triosephosphate isomerase, cytosolic OS=Oryza sati... [more]
TPIS_ARATH6.900e-1880.00Triosephosphate isomerase, cytosolic OS=Arabidopsi... [more]
TPIS_COPJA2.564e-1780.56Triosephosphate isomerase, cytosolic OS=Coptis jap... [more]
TPIS_SECCE2.564e-1790.91Triosephosphate isomerase, cytosolic OS=Secale cer... [more]
TPIS_MAIZE9.523e-1790.91Triosephosphate isomerase, cytosolic OS=Zea mays P... [more]
TPIS_HORVU7.761e-1684.85Triosephosphate isomerase, cytosolic OS=Hordeum vu... [more]
TPIS_MACMU2.341e-1482.76Triosephosphate isomerase OS=Macaca mulatta GN=TPI... [more]
TPIS_MACFA2.341e-1482.76Triosephosphate isomerase OS=Macaca fascicularis G... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_19336 Citrus sinensis phloem Citrus sinensis cDNA clone VPE-45_B07 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DR911208 ID=DR911208; Name=DR911208; organism=Citrus sinensis; type=EST; length=326bp
AGACAATGTCAGTGCTGAAGTTGCAGCATCAACTAGAATCATCTACGGAG
GTTCTGTAAATGGAGCAAATTGCAAAGAGATTGGCAGCACAACCTGATGT
GGATGGATTTTTGGTTGGTGGAGCTTCTCTAAAGCCGGAGTTCATCGATA
TTATCAAGTCTGCCACAGTGAAGAAGAATTGAAGTTAAAGACCAAGCTAC
TCGGCTTGCTGATGCCTGCAGATTTCTCTGTTATGCGTTCTTGAAACTAG
CGTTGTAATTTTTGTGGTCTGTTAAGTGTTACCAACGCCTGTTCCAGTAT
AATAAGTTAAATCTTCAGAATTCTTT
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