DR908756

Overview
NameDR908756
Unique NameDR908756
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length584
Libraries
Library NameType
Citrus sinensis phloemcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_ENTS8 (Methylthioribose kinase OS=Enterobacter sakazakii (strain ATCC BAA-894) GN=mtnK PE=3 SV=2)

HSP 1 Score: 99.7525 bits (247), Expect = 6.065e-30
Identity = 57/147 (38.78%), Postives = 85/147 (57.82%), Query Frame = 1 
Query:  142 QALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHIILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPYLDRDADAVREDNTLKLEVAGLKSMFCERAQ 582
            +A  L  H Q CP H  +++H+D T++++ M  L   H I R  LI G+ YP  A  + E++A+TLF+TS  Y      K  VA F  N E+C +TE + F+DPY+V + N++ +  L+ D  A+R+D  LK  VA LK  F   A+
Sbjct:   80 EAQTLVAHYQHCPAHTVKIHHYDPTLAVMVMEDLSD-HRIWRGELIKGVHYPQAARQLGEYLAQTLFHTSDFYLHPHAKKAEVARFI-NPEMCEITEDLFFNDPYQVHERNNYPAE-LEADVAALRDDAALKCAVAALKHRFFSHAE 223

HSP 2 Score: 47.7506 bits (112), Expect = 6.065e-30
Identity = 19/30 (63.33%), Postives = 25/30 (83.33%), Query Frame = 3 
Query:   66 VIKQALPYVRCIGESWPMTKERAYLPGTSI 155
            V+KQALPYVRC+GESWP+T +RA L   ++
Sbjct:   55 VVKQALPYVRCVGESWPLTLDRARLEAQTL 84

HSP 3 Score: 24.2534 bits (51), Expect = 6.065e-30
Identity = 9/12 (75.00%), Postives = 11/12 (91.67%), Query Frame = 2 
Query:    2 KEVGDGNLNFVY 37
            +EVGDGNLN V+
Sbjct:   32 QEVGDGNLNLVF 43
[Close]
BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_CITK8 (Methylthioribose kinase OS=Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) GN=mtnK PE=3 SV=2)

HSP 1 Score: 96.2857 bits (238), Expect = 6.362e-29
Identity = 56/147 (38.10%), Postives = 83/147 (56.46%), Query Frame = 1 
Query:  142 QALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHIILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPYLDRDADAVREDNTLKLEVAGLKSMFCERAQ 582
            +A  L  H Q CP H  +++HFD  ++++ M  L   H I R  LI G  YP  A  + E++A+TLF+TS  Y    + K  VA F  N E+C +TE + F+DPY++ + N + +  ++ D  A+R D  LKL VA LK  F   A+
Sbjct:   80 EAQTLVAHYQHCPQHTVKIHHFDPELAVMVMEDLSD-HNIWRGELIKGAWYPQAARQLGEYLAQTLFHTSDFYLHPHDKKAQVATFI-NPEMCEITEDLFFNDPYQIHERNSYPAE-IEGDVAALRSDAQLKLAVAALKHRFFSHAE 223

HSP 2 Score: 47.7506 bits (112), Expect = 6.362e-29
Identity = 19/30 (63.33%), Postives = 25/30 (83.33%), Query Frame = 3 
Query:   66 VIKQALPYVRCIGESWPMTKERAYLPGTSI 155
            V+KQALPYVRC+GESWP+T +RA L   ++
Sbjct:   55 VVKQALPYVRCVGESWPLTLDRARLEAQTL 84

HSP 3 Score: 24.2534 bits (51), Expect = 6.362e-29
Identity = 9/12 (75.00%), Postives = 11/12 (91.67%), Query Frame = 2 
Query:    2 KEVGDGNLNFVY 37
            +EVGDGNLN V+
Sbjct:   32 QEVGDGNLNLVF 43
[Close]
BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_ENT38 (Methylthioribose kinase OS=Enterobacter sp. (strain 638) GN=mtnK PE=3 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.808e-28
Identity = 53/147 (36.05%), Postives = 87/147 (59.18%), Query Frame = 1 
Query:  142 QALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHIILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPYLDRDADAVREDNTLKLEVAGLKSMFCERAQ 582
            +A  L EH Q  P H  +++H+D  ++++ M  L   H I R  LI+G+ YP  +  + E++A TLF+TS  Y      K  VA++  N E+C +TE + F+DPY++ + N++ +  L+ D  A+R D+ LK+ VA LK  F  +A+
Sbjct:   80 EAQTLVEHYQHSPQHTVKIHHYDPELAVMVMEDLSS-HKIWRGELISGVYYPQASRQLGEYLAHTLFHTSDFYLHPHAKKAQVAKYI-NPEMCEITEDLFFNDPYQIHERNNYPAE-LEADVAALRSDDQLKIAVASLKHRFFSQAE 223

HSP 2 Score: 47.3654 bits (111), Expect = 1.808e-28
Identity = 18/30 (60.00%), Postives = 25/30 (83.33%), Query Frame = 3 
Query:   66 VIKQALPYVRCIGESWPMTKERAYLPGTSI 155
            ++KQALPYVRC+GESWP+T +RA L   ++
Sbjct:   55 IVKQALPYVRCVGESWPLTLDRARLEAQTL 84

HSP 3 Score: 23.8682 bits (50), Expect = 1.808e-28
Identity = 8/12 (66.67%), Postives = 11/12 (91.67%), Query Frame = 2 
Query:    2 KEVGDGNLNFVY 37
            +E+GDGNLN V+
Sbjct:   32 QEIGDGNLNLVF 43
[Close]
BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_KLEP3 (Methylthioribose kinase OS=Klebsiella pneumoniae (strain 342) GN=mtnK PE=3 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 3.955e-28
Identity = 54/147 (36.73%), Postives = 84/147 (57.14%), Query Frame = 1 
Query:  142 QALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHIILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPYLDRDADAVREDNTLKLEVAGLKSMFCERAQ 582
            +A  L  H Q  P H  +++HFD  ++++ M  L   H I R  LIA + YP  A  + +++A+ LF+TS  Y    E K  VA+F  N  +C +TE + F+DPY++ + N++ +  L+ D  A+R+D  LKL VA LK  F   A+
Sbjct:   80 EAQTLVAHYQHSPQHTVKIHHFDPELAVMVMEDLSD-HRIWRGELIANVYYPQAARQLGDYLAQVLFHTSDFYLHPHEKKAQVAQF-SNPAMCEITEDLFFNDPYQIHERNNYPAE-LETDVAALRDDAQLKLAVAALKHRFFAHAE 223

HSP 2 Score: 47.3654 bits (111), Expect = 3.955e-28
Identity = 18/30 (60.00%), Postives = 25/30 (83.33%), Query Frame = 3 
Query:   66 VIKQALPYVRCIGESWPMTKERAYLPGTSI 155
            ++KQALPYVRC+GESWP+T +RA L   ++
Sbjct:   55 IVKQALPYVRCVGESWPLTLDRARLEAQTL 84

HSP 3 Score: 24.6386 bits (52), Expect = 3.955e-28
Identity = 10/14 (71.43%), Postives = 12/14 (85.71%), Query Frame = 2 
Query:    2 KEVGDGNLNFVYIV 43
            +EVGDGNLN V+ V
Sbjct:   32 QEVGDGNLNLVFKV 45
[Close]
BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_KLEPN (Methylthioribose kinase OS=Klebsiella pneumoniae GN=mtnK PE=1 SV=2)

HSP 1 Score: 93.2041 bits (230), Expect = 5.134e-28
Identity = 54/147 (36.73%), Postives = 84/147 (57.14%), Query Frame = 1 
Query:  142 QALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHIILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPYLDRDADAVREDNTLKLEVAGLKSMFCERAQ 582
            +A  L  H Q  P H  +++HFD  ++++ M  L   H I R  LIA + YP  A  + +++A+ LF+TS  Y    E K  VA+F  N  +C +TE + F+DPY++ + N++ +  L+ D  A+R+D  LKL VA LK  F   A+
Sbjct:   80 EAQTLVAHYQHSPQHTVKIHHFDPELAVMVMEDLSD-HRIWRGELIANVYYPQAARQLGDYLAQVLFHTSDFYLHPHEKKAQVAQFI-NPAMCEITEDLFFNDPYQIHERNNYPAE-LEADVAALRDDAQLKLAVAALKHRFFAHAE 223

HSP 2 Score: 47.3654 bits (111), Expect = 5.134e-28
Identity = 18/30 (60.00%), Postives = 25/30 (83.33%), Query Frame = 3 
Query:   66 VIKQALPYVRCIGESWPMTKERAYLPGTSI 155
            ++KQALPYVRC+GESWP+T +RA L   ++
Sbjct:   55 IVKQALPYVRCVGESWPLTLDRARLEAQTL 84

HSP 3 Score: 24.6386 bits (52), Expect = 5.134e-28
Identity = 10/14 (71.43%), Postives = 12/14 (85.71%), Query Frame = 2 
Query:    2 KEVGDGNLNFVYIV 43
            +EVGDGNLN V+ V
Sbjct:   32 QEVGDGNLNLVFKV 45
[Close]
BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_KLEP7 (Methylthioribose kinase OS=Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) GN=mtnK PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 5.134e-28
Identity = 54/147 (36.73%), Postives = 84/147 (57.14%), Query Frame = 1 
Query:  142 QALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHIILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPYLDRDADAVREDNTLKLEVAGLKSMFCERAQ 582
            +A  L  H Q  P H  +++HFD  ++++ M  L   H I R  LIA + YP  A  + +++A+ LF+TS  Y    E K  VA+F  N  +C +TE + F+DPY++ + N++ +  L+ D  A+R+D  LKL VA LK  F   A+
Sbjct:   80 EAQTLVAHYQHSPQHTVKIHHFDPELAVMVMEDLSD-HRIWRGELIANVYYPQAARQLGDYLAQVLFHTSDFYLHPHEKKAQVAQFI-NPAMCEITEDLFFNDPYQIHERNNYPAE-LEADVAALRDDAQLKLAVAALKHRFFAHAE 223

HSP 2 Score: 47.3654 bits (111), Expect = 5.134e-28
Identity = 18/30 (60.00%), Postives = 25/30 (83.33%), Query Frame = 3 
Query:   66 VIKQALPYVRCIGESWPMTKERAYLPGTSI 155
            ++KQALPYVRC+GESWP+T +RA L   ++
Sbjct:   55 IVKQALPYVRCVGESWPLTLDRARLEAQTL 84

HSP 3 Score: 24.6386 bits (52), Expect = 5.134e-28
Identity = 10/14 (71.43%), Postives = 12/14 (85.71%), Query Frame = 2 
Query:    2 KEVGDGNLNFVYIV 43
            +EVGDGNLN V+ V
Sbjct:   32 QEVGDGNLNLVFKV 45
[Close]
BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_SERP5 (Methylthioribose kinase OS=Serratia proteamaculans (strain 568) GN=mtnK PE=3 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 6.664e-28
Identity = 57/147 (38.78%), Postives = 82/147 (55.78%), Query Frame = 1 
Query:  142 QALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHIILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPYLDRDADAVREDNTLKLEVAGLKSMFCERAQ 582
            +A  L  HG  CP H  +V H D  ++++    L   HI  R  L+ G  YPL A  ++E++A+TLF+TS  Y+T  E K  V+ F  N ELC++TE + F+DPY   + N +  P L      +R D  L+L VAGLK  F  +A+
Sbjct:   80 EAETLLIHGGFCPRHTVKVLHHDAELAVMVQEDLSDHHI-WRSELVKGHYYPLAAGQLAEYLAQTLFHTSDFYQTAQEKKAEVSRF-SNPELCQITEDLFFTDPYIDHERNQF-DPALLPQVLELRNDAALRLAVAGLKHRFLSKAE 223

HSP 2 Score: 46.595 bits (109), Expect = 6.664e-28
Identity = 17/30 (56.67%), Postives = 25/30 (83.33%), Query Frame = 3 
Query:   66 VIKQALPYVRCIGESWPMTKERAYLPGTSI 155
            ++KQALPYVRC+GESWP+T +RA +   ++
Sbjct:   55 IVKQALPYVRCVGESWPLTLDRARIEAETL 84

HSP 3 Score: 23.483 bits (49), Expect = 6.664e-28
Identity = 8/11 (72.73%), Postives = 10/11 (90.91%), Query Frame = 2 
Query:    5 EVGDGNLNFVY 37
            E+GDGNLN V+
Sbjct:   33 EIGDGNLNLVF 43
[Close]
BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_ERWT9 (Methylthioribose kinase OS=Erwinia tasmaniensis (strain DSM 17950 / Et1/99) GN=mtnK PE=3 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 8.651e-28
Identity = 53/147 (36.05%), Postives = 87/147 (59.18%), Query Frame = 1 
Query:  142 QALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHIILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPYLDRDADAVREDNTLKLEVAGLKSMFCERAQ 582
            +A  L EHG+ CP H   + H+D  +++  M  L   H I R  L+ GI +P  A  + +++A+TLF+TS  ++   + K +V  F  N ELC +TE++ F++PY+V   N +    L+  A+++RED+ L++ VAGLK  F   A+
Sbjct:   80 EAEVLIEHGKFCPQHTVNILHYDPLLAVTVMEDLS-DHAIWRADLVKGIDWPQAAGQLGDYLAQTLFHTSDFFQHPHQKKADVIRFT-NPELCDITEELFFNEPYEVHARNAYPRA-LEPLAESLREDHELRVAVAGLKHRFYSNAE 223

HSP 2 Score: 45.0542 bits (105), Expect = 8.651e-28
Identity = 17/25 (68.00%), Postives = 23/25 (92.00%), Query Frame = 3 
Query:   66 VIKQALPYVRCIGESWPMTKERAYL 140
            ++KQALP+VRC+GESWP+T +RA L
Sbjct:   55 IVKQALPWVRCVGESWPLTLDRARL 79

HSP 3 Score: 23.8682 bits (50), Expect = 8.651e-28
Identity = 9/11 (81.82%), Postives = 10/11 (90.91%), Query Frame = 2 
Query:    5 EVGDGNLNFVY 37
            EVGDGNLN V+
Sbjct:   33 EVGDGNLNLVF 43
[Close]
BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_YERE8 (Methylthioribose kinase OS=Yersinia enterocolitica serotype O:8 / biotype 1B (strain 8081) GN=mtnK PE=3 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 1.458e-27
Identity = 55/147 (37.41%), Postives = 84/147 (57.14%), Query Frame = 1 
Query:  142 QALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHIILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPYLDRDADAVREDNTLKLEVAGLKSMFCERAQ 582
            +A  L  HGQ CP H   V H D  ++++    L   H I R  L+ G  YP  A  ++E++A+TLF+TS  Y++    K  V+ +  N ELC++TE + F+DPY   + N++  P L  +  A+R+D+ LKL VA LK  F  +A+
Sbjct:   80 EAETLLTHGQFCPQHTVNVLHHDAELAVMVQEDLSD-HEIWRSELVKGKYYPQAAGQLAEYLAQTLFHTSDFYQSAQAKKAAVSRYT-NPELCQITEDLFFTDPYIDHERNNF-DPALLPEVLALRQDDALKLAVASLKHRFLSKAE 223

HSP 2 Score: 46.595 bits (109), Expect = 1.458e-27
Identity = 17/30 (56.67%), Postives = 25/30 (83.33%), Query Frame = 3 
Query:   66 VIKQALPYVRCIGESWPMTKERAYLPGTSI 155
            ++KQALPYVRC+GESWP+T +RA +   ++
Sbjct:   55 IVKQALPYVRCVGESWPLTLDRARIEAETL 84

HSP 3 Score: 23.483 bits (49), Expect = 1.458e-27
Identity = 8/11 (72.73%), Postives = 10/11 (90.91%), Query Frame = 2 
Query:    5 EVGDGNLNFVY 37
            E+GDGNLN V+
Sbjct:   33 EIGDGNLNLVF 43
[Close]
BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_YERPY (Methylthioribose kinase OS=Yersinia pseudotuberculosis serotype O:3 (strain YPIII) GN=mtnK PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.891e-27
Identity = 55/147 (37.41%), Postives = 83/147 (56.46%), Query Frame = 1 
Query:  142 QALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHIILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPYLDRDADAVREDNTLKLEVAGLKSMFCERAQ 582
            +A  L  H Q CP H  +V H D  ++++    L   HI  R  LI G  YP  AE + E++A+TLF+TS  Y++    K  V+ +  N ELC++TE + F+DPY   + N++  P L  +  ++R+D  LKL VA LK  F  +A+
Sbjct:   80 EAETLLTHSQFCPQHTVKVLHHDAELAVMVQEDLSDHHI-WRHELIQGNYYPQAAEQLGEYLAQTLFHTSDFYQSAQAKKAAVSRYT-NPELCQITEDLFFTDPYIEHERNNF-DPVLLPEVLSLRQDKALKLAVASLKHRFLSQAE 223

HSP 2 Score: 46.595 bits (109), Expect = 1.891e-27
Identity = 17/30 (56.67%), Postives = 25/30 (83.33%), Query Frame = 3 
Query:   66 VIKQALPYVRCIGESWPMTKERAYLPGTSI 155
            ++KQALPYVRC+GESWP+T +RA +   ++
Sbjct:   55 IVKQALPYVRCVGESWPLTLDRARIEAETL 84

HSP 3 Score: 23.483 bits (49), Expect = 1.891e-27
Identity = 8/11 (72.73%), Postives = 10/11 (90.91%), Query Frame = 2 
Query:    5 EVGDGNLNFVY 37
            E+GDGNLN V+
Sbjct:   33 EIGDGNLNLVF 43
The following BLAST results are available for this feature:
BLAST of DR908756 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 41
Match NameE-valueIdentityDescription
MTNK_ENTS86.065e-3038.78Methylthioribose kinase OS=Enterobacter sakazakii ... [more]
MTNK_CITK86.362e-2938.10Methylthioribose kinase OS=Citrobacter koseri (str... [more]
MTNK_ENT381.808e-2836.05Methylthioribose kinase OS=Enterobacter sp. (strai... [more]
MTNK_KLEP33.955e-2836.73Methylthioribose kinase OS=Klebsiella pneumoniae (... [more]
MTNK_KLEPN5.134e-2836.73Methylthioribose kinase OS=Klebsiella pneumoniae G... [more]
MTNK_KLEP75.134e-2836.73Methylthioribose kinase OS=Klebsiella pneumoniae s... [more]
MTNK_SERP56.664e-2838.78Methylthioribose kinase OS=Serratia proteamaculans... [more]
MTNK_ERWT98.651e-2836.05Methylthioribose kinase OS=Erwinia tasmaniensis (s... [more]
MTNK_YERE81.458e-2737.41Methylthioribose kinase OS=Yersinia enterocolitica... [more]
MTNK_YERPY1.891e-2737.41Methylthioribose kinase OS=Yersinia pseudotubercul... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_16884 Citrus sinensis phloem Citrus sinensis cDNA clone VPE-41_F04 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DR908756 ID=DR908756; Name=DR908756; organism=Citrus sinensis; type=EST; length=584bp
CAAAGAAGTCGGAGACGGCAACCTTAATTTCGTCTACATCGTCTGTCGGC
TCTTCTGGTTCTTTCGTCATCAAGCAGGCTCTTCCGTATGTACGTTGTAT
CGGTGAATCATGGCCCATGACAAAGGAAAGAGCGTATTTGCCAGGCACTA
GCATTGAAAGAGCATGGCCAATTGTGTCCTGACCATGTTCCCGAAGTCTA
TCATTTTGACCGTACAATGTCTTTGATTGGTATGCGTTACCTGGAGCCTC
CTCATATTATCCTGAGAAAGGGGTTGATTGCTGGAATAAAGTATCCATTA
CTTGCGGAACACATGTCAGAATTCATGGCAAAGACTCTTTTCTACACATC
TCTTTTATATCGTACCACCACAGAGCACAAACGTAATGTTGCTGAATTTT
GTGGGAATGTGGAGCTATGCAGGCTCACGGAGCAGGTTGTTTTCTCGGAC
CCCTACAAAGTATCTAAGTTTAACCATTGGACTTCTCCTTATCTTGATCG
TGACGCTGACGCTGTTCGGGAGGATAATACATTAAAGCTGGAAGTTGCTG
GGTTGAAATCCATGTTCTGTGAAAGAGCACAAGC
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