CN189327

Overview
NameCN189327
Unique NameCN189327
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length755
Libraries
Library NameType
Washington Navel Orange Stored Fruit Rind cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN189327 vs. ExPASy Swiss-Prot
Match: IDHP_MEDSA (Isocitrate dehydrogenase [NADP], chloroplastic (Fragment) OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 1.734e-44
Identity = 82/91 (90.11%), Postives = 87/91 (95.60%), Query Frame = 2 
Query:  407 RYNVAIKCATITPDEVRVKEFNLKRMWKSPNGTIRNILNGTVFREPIICKNIPRLVSGWTKPICIGRHAFGDQYRATDAVIQGPGKLKLVF 679
            +YNVAIKCATITPDE RVKEF LK MW+SPNGTIRNILNGTVFREPIICKNIPRL+ GWTKPICIGRHAFGDQYRATD+VI+GPGKLKLVF
Sbjct:   89 KYNVAIKCATITPDEARVKEFGLKSMWRSPNGTIRNILNGTVFREPIICKNIPRLIPGWTKPICIGRHAFGDQYRATDSVIKGPGKLKLVF 179

HSP 2 Score: 120.168 bits (300), Expect = 1.248e-26
Identity = 57/69 (82.61%), Postives = 64/69 (92.75%), Query Frame = 1 
Query:   10 MEFQKIKVANPIVEMDGDEMTRVIWKLIKDKLINPFLELDIKYFDLGLPNRDATDDQVTIQSAQATLKY 216
            M FQKIKVANPIVEMDGDEMTR+IWK IKDKLI PF+ELDIKYFDLGLP RD T+D+VT++SA+ATLKY
Sbjct:   22 MGFQKIKVANPIVEMDGDEMTRIIWKYIKDKLIFPFVELDIKYFDLGLPYRDETNDKVTVESAEATLKY 90
[Close]
BLAST of CN189327 vs. ExPASy Swiss-Prot
Match: IDHC_TOBAC (Isocitrate dehydrogenase [NADP] OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 179.104 bits (453), Expect = 2.264e-44
Identity = 83/91 (91.21%), Postives = 86/91 (94.51%), Query Frame = 2 
Query:  407 RYNVAIKCATITPDEVRVKEFNLKRMWKSPNGTIRNILNGTVFREPIICKNIPRLVSGWTKPICIGRHAFGDQYRATDAVIQGPGKLKLVF 679
            +YNVAIKCATITPDE RVKEFNLK MW+SPNGTIRNILNGTVFREPI+CKNIPRLV GWTKPICIGRHAFGDQYRATD VIQG GKLKLVF
Sbjct:   68 KYNVAIKCATITPDEARVKEFNLKSMWRSPNGTIRNILNGTVFREPIMCKNIPRLVPGWTKPICIGRHAFGDQYRATDTVIQGAGKLKLVF 158

HSP 2 Score: 120.553 bits (301), Expect = 9.558e-27
Identity = 58/69 (84.06%), Postives = 63/69 (91.30%), Query Frame = 1 
Query:   10 MEFQKIKVANPIVEMDGDEMTRVIWKLIKDKLINPFLELDIKYFDLGLPNRDATDDQVTIQSAQATLKY 216
            M F KIKV NPIVEMDGDEMTRVIWK IKDKLI PFLELDIKYFDLGLP+RDATDD+VT++SA+AT KY
Sbjct:    1 MTFDKIKVENPIVEMDGDEMTRVIWKSIKDKLICPFLELDIKYFDLGLPHRDATDDKVTVESAEATQKY 69
[Close]
BLAST of CN189327 vs. ExPASy Swiss-Prot
Match: IDHC_SOLTU (Isocitrate dehydrogenase [NADP] OS=Solanum tuberosum GN=ICDH-1 PE=2 SV=1)

HSP 1 Score: 177.563 bits (449), Expect = 6.589e-44
Identity = 82/91 (90.11%), Postives = 86/91 (94.51%), Query Frame = 2 
Query:  407 RYNVAIKCATITPDEVRVKEFNLKRMWKSPNGTIRNILNGTVFREPIICKNIPRLVSGWTKPICIGRHAFGDQYRATDAVIQGPGKLKLVF 679
            +YNVAIKCATITPDE RVKEFNLK MW+SPNGTIRNILNGTVFREPI+CKNIPRLV GWTKPICIGRHAFGDQYRATD VI+G GKLKLVF
Sbjct:   68 KYNVAIKCATITPDEARVKEFNLKSMWRSPNGTIRNILNGTVFREPIMCKNIPRLVPGWTKPICIGRHAFGDQYRATDTVIKGAGKLKLVF 158

HSP 2 Score: 117.857 bits (294), Expect = 6.195e-26
Identity = 57/69 (82.61%), Postives = 62/69 (89.86%), Query Frame = 1 
Query:   10 MEFQKIKVANPIVEMDGDEMTRVIWKLIKDKLINPFLELDIKYFDLGLPNRDATDDQVTIQSAQATLKY 216
            M FQKI V NPIVEMDGDEMTRVIWK IKDKLI PFLELDIKYF LGLP+RDATDD+VT++SA+AT KY
Sbjct:    1 MAFQKITVQNPIVEMDGDEMTRVIWKSIKDKLILPFLELDIKYFSLGLPHRDATDDKVTVESAEATQKY 69
[Close]
BLAST of CN189327 vs. ExPASy Swiss-Prot
Match: IDHC_SOYBN (Isocitrate dehydrogenase [NADP] OS=Glycine max GN=IDH1 PE=2 SV=2)

HSP 1 Score: 173.711 bits (439), Expect = 9.514e-43
Identity = 81/91 (89.01%), Postives = 84/91 (92.31%), Query Frame = 2 
Query:  407 RYNVAIKCATITPDEVRVKEFNLKRMWKSPNGTIRNILNGTVFREPIICKNIPRLVSGWTKPICIGRHAFGDQYRATDAVIQGPGKLKLVF 679
            +YNVAIKCATITPDE RVKEF LK MWKSPNGTIRNILNGTVFREPI+CKNIPRLV GWTK ICIGRHAFGDQYRATD VI+G GKLKLVF
Sbjct:   69 KYNVAIKCATITPDEARVKEFGLKSMWKSPNGTIRNILNGTVFREPILCKNIPRLVPGWTKAICIGRHAFGDQYRATDTVIKGAGKLKLVF 159

HSP 2 Score: 121.324 bits (303), Expect = 5.604e-27
Identity = 59/67 (88.06%), Postives = 63/67 (94.03%), Query Frame = 1 
Query:   16 FQKIKVANPIVEMDGDEMTRVIWKLIKDKLINPFLELDIKYFDLGLPNRDATDDQVTIQSAQATLKY 216
            FQKIKVANPIVEMDGDEMTRVIWK IKDKLI PFLELDIKY+DLGLP RD TDD+VTI+SA+ATLKY
Sbjct:    4 FQKIKVANPIVEMDGDEMTRVIWKSIKDKLILPFLELDIKYYDLGLPYRDETDDKVTIESAEATLKY 70
[Close]
BLAST of CN189327 vs. ExPASy Swiss-Prot
Match: IDHC_PONAB (Isocitrate dehydrogenase [NADP] cytoplasmic OS=Pongo abelii GN=IDH1 PE=2 SV=2)

HSP 1 Score: 158.688 bits (400), Expect = 3.167e-38
Identity = 71/91 (78.02%), Postives = 81/91 (89.01%), Query Frame = 2 
Query:  407 RYNVAIKCATITPDEVRVKEFNLKRMWKSPNGTIRNILNGTVFREPIICKNIPRLVSGWTKPICIGRHAFGDQYRATDAVIQGPGKLKLVF 679
            +YNV +KCATITPDE RV+EF LK+MWKSPNGTIRNIL GTVFRE IICKNIPRLVSGW KPI IGRHA+GDQYRATD V+ GPGK+++ +
Sbjct:   66 KYNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVSGWVKPIIIGRHAYGDQYRATDFVVPGPGKVEITY 156

HSP 2 Score: 82.4185 bits (202), Expect = 2.885e-15
Identity = 37/58 (63.79%), Postives = 49/58 (84.48%), Query Frame = 1 
Query:   43 IVEMDGDEMTRVIWKLIKDKLINPFLELDIKYFDLGLPNRDATDDQVTIQSAQATLKY 216
            +VEM GDEMTR+IW+LIK+KLI P++ELD+  +DLG+ NRDAT+DQVT  +A+A  KY
Sbjct:   10 VVEMQGDEMTRIIWELIKEKLIFPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKY 67
[Close]
BLAST of CN189327 vs. ExPASy Swiss-Prot
Match: IDHC_MICME (Isocitrate dehydrogenase [NADP] cytoplasmic OS=Microtus mexicanus GN=IDH1 PE=2 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 3.167e-38
Identity = 71/91 (78.02%), Postives = 81/91 (89.01%), Query Frame = 2 
Query:  407 RYNVAIKCATITPDEVRVKEFNLKRMWKSPNGTIRNILNGTVFREPIICKNIPRLVSGWTKPICIGRHAFGDQYRATDAVIQGPGKLKLVF 679
            +YNV +KCATITPDE RV+EF LK+MWKSPNGTIRNIL GTVFRE IICKNIPRLV+GW KPI IGRHA+GDQYRATD V+ GPGK+++ F
Sbjct:   66 KYNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWVKPIIIGRHAYGDQYRATDFVVPGPGKVEITF 156

HSP 2 Score: 82.4185 bits (202), Expect = 2.885e-15
Identity = 37/58 (63.79%), Postives = 49/58 (84.48%), Query Frame = 1 
Query:   43 IVEMDGDEMTRVIWKLIKDKLINPFLELDIKYFDLGLPNRDATDDQVTIQSAQATLKY 216
            +VEM GDEMTR+IW+LIK+KLI P++ELD+  +DLG+ NRDAT+DQVT  +A+A  KY
Sbjct:   10 VVEMQGDEMTRIIWELIKEKLILPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKY 67
[Close]
BLAST of CN189327 vs. ExPASy Swiss-Prot
Match: IDHC_SHEEP (Isocitrate dehydrogenase [NADP] cytoplasmic OS=Ovis aries GN=IDH1 PE=2 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 4.136e-38
Identity = 71/91 (78.02%), Postives = 81/91 (89.01%), Query Frame = 2 
Query:  407 RYNVAIKCATITPDEVRVKEFNLKRMWKSPNGTIRNILNGTVFREPIICKNIPRLVSGWTKPICIGRHAFGDQYRATDAVIQGPGKLKLVF 679
            +YNV +KCATITPDE RV+EF LK+MWKSPNGTIRNIL GTVFRE IICKNIPRLVSGW KPI IGRHA+GDQYRATD V+ GPGK+++ +
Sbjct:   66 KYNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVSGWVKPIIIGRHAYGDQYRATDFVVPGPGKVEICY 156

HSP 2 Score: 78.9518 bits (193), Expect = 3.189e-14
Identity = 35/58 (60.34%), Postives = 48/58 (82.76%), Query Frame = 1 
Query:   43 IVEMDGDEMTRVIWKLIKDKLINPFLELDIKYFDLGLPNRDATDDQVTIQSAQATLKY 216
            +VEM GDEMTR+IW+LIK+KLI P+++LD+  +DL + NRDAT+DQVT  +A+A  KY
Sbjct:   10 VVEMQGDEMTRIIWELIKEKLIFPYVDLDLHSYDLSIENRDATNDQVTKDAAEAIKKY 67
[Close]
BLAST of CN189327 vs. ExPASy Swiss-Prot
Match: IDHC_BOVIN (Isocitrate dehydrogenase [NADP] cytoplasmic OS=Bos taurus GN=IDH1 PE=2 SV=1)

HSP 1 Score: 157.918 bits (398), Expect = 5.402e-38
Identity = 71/91 (78.02%), Postives = 81/91 (89.01%), Query Frame = 2 
Query:  407 RYNVAIKCATITPDEVRVKEFNLKRMWKSPNGTIRNILNGTVFREPIICKNIPRLVSGWTKPICIGRHAFGDQYRATDAVIQGPGKLKLVF 679
            +YNV +KCATITPDE RV+EF LK+MWKSPNGTIRNIL GTVFRE IICKNIPRLVSGW KPI IGRHA+GDQYRATD V+ GPGK+++ +
Sbjct:   66 KYNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVSGWVKPIIIGRHAYGDQYRATDFVVPGPGKVEISY 156

HSP 2 Score: 83.1889 bits (204), Expect = 1.691e-15
Identity = 40/66 (60.61%), Postives = 53/66 (80.30%), Query Frame = 1 
Query:   19 QKIKVANPIVEMDGDEMTRVIWKLIKDKLINPFLELDIKYFDLGLPNRDATDDQVTIQSAQATLKY 216
            QKI+    +VEM GDEMTR+IW+LIK+KLI P++ELD+  +DLG+ NRDAT+DQVT  +A+A  KY
Sbjct:    3 QKIQ-GGSVVEMQGDEMTRIIWELIKEKLIFPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKY 67
[Close]
BLAST of CN189327 vs. ExPASy Swiss-Prot
Match: IDHC_RAT (Isocitrate dehydrogenase [NADP] cytoplasmic OS=Rattus norvegicus GN=Idh1 PE=1 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 7.056e-38
Identity = 70/91 (76.92%), Postives = 81/91 (89.01%), Query Frame = 2 
Query:  407 RYNVAIKCATITPDEVRVKEFNLKRMWKSPNGTIRNILNGTVFREPIICKNIPRLVSGWTKPICIGRHAFGDQYRATDAVIQGPGKLKLVF 679
            +YNV +KCATITPDE RV+EF LK+MWKSPNGTIRNIL GTVFRE IICKNIPRLV+GW KPI IGRHA+GDQYRATD V+ GPGK+++ +
Sbjct:   66 KYNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWVKPIIIGRHAYGDQYRATDFVVPGPGKVEITY 156

HSP 2 Score: 82.4185 bits (202), Expect = 2.885e-15
Identity = 37/58 (63.79%), Postives = 49/58 (84.48%), Query Frame = 1 
Query:   43 IVEMDGDEMTRVIWKLIKDKLINPFLELDIKYFDLGLPNRDATDDQVTIQSAQATLKY 216
            +VEM GDEMTR+IW+LIK+KLI P++ELD+  +DLG+ NRDAT+DQVT  +A+A  KY
Sbjct:   10 VVEMQGDEMTRIIWELIKEKLILPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKY 67
[Close]
BLAST of CN189327 vs. ExPASy Swiss-Prot
Match: IDHC_MOUSE (Isocitrate dehydrogenase [NADP] cytoplasmic OS=Mus musculus GN=Idh1 PE=1 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 7.056e-38
Identity = 70/91 (76.92%), Postives = 81/91 (89.01%), Query Frame = 2 
Query:  407 RYNVAIKCATITPDEVRVKEFNLKRMWKSPNGTIRNILNGTVFREPIICKNIPRLVSGWTKPICIGRHAFGDQYRATDAVIQGPGKLKLVF 679
            +YNV +KCATITPDE RV+EF LK+MWKSPNGTIRNIL GTVFRE IICKNIPRLV+GW KPI IGRHA+GDQYRATD V+ GPGK+++ +
Sbjct:   66 KYNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWVKPIIIGRHAYGDQYRATDFVVPGPGKVEITY 156

HSP 2 Score: 82.4185 bits (202), Expect = 2.885e-15
Identity = 37/58 (63.79%), Postives = 49/58 (84.48%), Query Frame = 1 
Query:   43 IVEMDGDEMTRVIWKLIKDKLINPFLELDIKYFDLGLPNRDATDDQVTIQSAQATLKY 216
            +VEM GDEMTR+IW+LIK+KLI P++ELD+  +DLG+ NRDAT+DQVT  +A+A  KY
Sbjct:   10 VVEMQGDEMTRIIWELIKEKLILPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKY 67
The following BLAST results are available for this feature:
BLAST of CN189327 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 30
Match NameE-valueIdentityDescription
IDHP_MEDSA1.734e-4490.11Isocitrate dehydrogenase [NADP], chloroplastic (Fr... [more]
IDHC_TOBAC2.264e-4491.21Isocitrate dehydrogenase [NADP] OS=Nicotiana tabac... [more]
IDHC_SOLTU6.589e-4490.11Isocitrate dehydrogenase [NADP] OS=Solanum tuberos... [more]
IDHC_SOYBN9.514e-4389.01Isocitrate dehydrogenase [NADP] OS=Glycine max GN=... [more]
IDHC_PONAB3.167e-3878.02Isocitrate dehydrogenase [NADP] cytoplasmic OS=Pon... [more]
IDHC_MICME3.167e-3878.02Isocitrate dehydrogenase [NADP] cytoplasmic OS=Mic... [more]
IDHC_SHEEP4.136e-3878.02Isocitrate dehydrogenase [NADP] cytoplasmic OS=Ovi... [more]
IDHC_BOVIN5.402e-3878.02Isocitrate dehydrogenase [NADP] cytoplasmic OS=Bos... [more]
IDHC_RAT7.056e-3876.92Isocitrate dehydrogenase [NADP] cytoplasmic OS=Rat... [more]
IDHC_MOUSE7.056e-3876.92Isocitrate dehydrogenase [NADP] cytoplasmic OS=Mus... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS06_0001K24_r Washington Navel Orange Stored Fruit Rind cDNA Library Citrus sinensis cDNA clone CS_WEc0001K24, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN189327 ID=CN189327; Name=CN189327; organism=Citrus sinensis; type=EST; length=755bp
GAGGGAGAAATGGAATTCCAGAAGATCAAGGTGGCCAATCCCATCGTTGA
AATGGACGGTGATGAAATGACTCGAGTTATTTGGAAACTCATCAAGGATA
AGCTAATTAATCCGTTTCTGGAGTTGGATATCAAGTACTTTGACCTTGGC
CTTCCCAACCGTGATGCCACCGACGATCAAGTTACCATTCAAAGCGCTCA
AGCTACTCTCAAGTACCTCTTTTTCTCTATGTTTCTGATCATAATCCTAA
TCATGTGGCCGCTGTTGTATTGTCCTTGTAAATTGTAATTGAGAGTATTT
ATAAACTTTAACCTGTTTTGCTGCTACGCCTTTGCCATTAGTTTATTTAT
ATCACTTCCTTCACTCTTTTGTGTGTTCAGTTTTCTACTTTTGCTCTTGT
TATTTTAGGTATAACGTAGCTATTAAGTGTGCAACTATCACTCCAGATGA
AGTGCGGGTTAAGGAATTTAACTTGAAAAGAATGTGGAAGAGTCCTAATG
GAACAATTCGCAACATTCTAAATGGCACAGTTTTTAGAGAACCGATTATC
TGTAAAAACATCCCCAGGCTGGTCTCAGGTTGGACTAAGCCAATCTGCAT
TGGGAGGCATGCTTTTGGTGATCAGTATCGAGCAACTGATGCTGTTATAC
AAGGGCCCGGGAAACTTAAGTTAGTCTTTGGCAATTGCCATGTTTGCAGT
TCCCGATGGACTTAATCAGAAGACAGAGTGGGAGGTCTACAGTTTCACTG
GTGCT
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