EY757381

Overview
NameEY757381
Unique NameEY757381
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length788
Libraries
Library NameType
Sweet orange leaf, field plant Bcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY757381 vs. ExPASy Swiss-Prot
Match: PSA5_SOYBN (Proteasome subunit alpha type-5 OS=Glycine max GN=PAE1 PE=2 SV=1)

HSP 1 Score: 263.462 bits (672), Expect = 1.166e-76
Identity = 131/137 (95.62%), Postives = 134/137 (97.81%), Query Frame = 2 
Query:   80 FSYGEPMTVESTTQALCDLALRFGEGDEESMSRPFGVSLLIAGSDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIARVAPTDHLYTPSEVEAVINRL 490
            FSYGEPMTVESTTQALCDLALRFGEGDEESMSRPFGVSLLIAG DENGPSLYYTDPSGTFWQCN KAIGSGSEGADSSLQEQ+NKDLTLQEAETIALSILKQVMEEKVTPNNVDIA+VAPT HLYTPSEVEAVI+RL
Sbjct:  101 FSYGEPMTVESTTQALCDLALRFGEGDEESMSRPFGVSLLIAGHDENGPSLYYTDPSGTFWQCNGKAIGSGSEGADSSLQEQFNKDLTLQEAETIALSILKQVMEEKVTPNNVDIAKVAPTYHLYTPSEVEAVISRL 237

HSP 2 Score: 45.0542 bits (105), Expect = 1.166e-76
Identity = 21/21 (100.00%), Postives = 21/21 (100.00%), Query Frame = 1 
Query:   13 SGLIADARTLVEHARVETQNH 75
            SGLIADARTLVEHARVETQNH
Sbjct:   79 SGLIADARTLVEHARVETQNH 99
[Close]
BLAST of EY757381 vs. ExPASy Swiss-Prot
Match: PSA5_ORYSJ (Proteasome subunit alpha type-5 OS=Oryza sativa subsp. japonica GN=PAE1 PE=2 SV=1)

HSP 1 Score: 258.84 bits (660), Expect = 2.826e-75
Identity = 128/137 (93.43%), Postives = 133/137 (97.08%), Query Frame = 2 
Query:   80 FSYGEPMTVESTTQALCDLALRFGEGDEESMSRPFGVSLLIAGSDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIARVAPTDHLYTPSEVEAVINRL 490
            FSYGEPMTVESTTQA+CDLALRFGEGDEESMSRPFGVSLLIAG DENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQYNK+LTLQEAETIALSILKQVMEEKVTPNNVDIA+V+P  HLYTP+EVEAVI RL
Sbjct:  101 FSYGEPMTVESTTQAICDLALRFGEGDEESMSRPFGVSLLIAGHDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQYNKELTLQEAETIALSILKQVMEEKVTPNNVDIAKVSPNYHLYTPAEVEAVIARL 237

HSP 2 Score: 45.0542 bits (105), Expect = 2.826e-75
Identity = 21/21 (100.00%), Postives = 21/21 (100.00%), Query Frame = 1 
Query:   13 SGLIADARTLVEHARVETQNH 75
            SGLIADARTLVEHARVETQNH
Sbjct:   79 SGLIADARTLVEHARVETQNH 99
[Close]
BLAST of EY757381 vs. ExPASy Swiss-Prot
Match: PSA5B_ARATH (Proteasome subunit alpha type-5-B OS=Arabidopsis thaliana GN=PAE2 PE=1 SV=2)

HSP 1 Score: 258.07 bits (658), Expect = 4.808e-75
Identity = 127/137 (92.70%), Postives = 133/137 (97.08%), Query Frame = 2 
Query:   80 FSYGEPMTVESTTQALCDLALRFGEGDEESMSRPFGVSLLIAGSDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIARVAPTDHLYTPSEVEAVINRL 490
            FSYGEPMTVESTTQALCDLALRFGEG+EESMSRPFGVSLLIAG DENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQ+NKD+TLQEAETIA+SILKQVMEEKVTPNNVDIA+VAP  HLYTP EVEAVI+RL
Sbjct:  101 FSYGEPMTVESTTQALCDLALRFGEGEEESMSRPFGVSLLIAGHDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQFNKDITLQEAETIAVSILKQVMEEKVTPNNVDIAKVAPAYHLYTPQEVEAVISRL 237

HSP 2 Score: 45.0542 bits (105), Expect = 4.808e-75
Identity = 21/21 (100.00%), Postives = 21/21 (100.00%), Query Frame = 1 
Query:   13 SGLIADARTLVEHARVETQNH 75
            SGLIADARTLVEHARVETQNH
Sbjct:   79 SGLIADARTLVEHARVETQNH 99
[Close]
BLAST of EY757381 vs. ExPASy Swiss-Prot
Match: PSA5A_ARATH (Proteasome subunit alpha type-5-A OS=Arabidopsis thaliana GN=PAE1 PE=1 SV=1)

HSP 1 Score: 256.144 bits (653), Expect = 1.814e-74
Identity = 127/137 (92.70%), Postives = 132/137 (96.35%), Query Frame = 2 
Query:   80 FSYGEPMTVESTTQALCDLALRFGEGDEESMSRPFGVSLLIAGSDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIARVAPTDHLYTPSEVEAVINRL 490
            FSYGEPMTVESTTQALCDLALRFGEG+EESMSRPFGVSLLIAG DENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQ+NKDL+LQEAETIA+SILKQVMEEKVTPNNVDIA+VAP  HLYTP EVEAVI RL
Sbjct:  101 FSYGEPMTVESTTQALCDLALRFGEGEEESMSRPFGVSLLIAGHDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQFNKDLSLQEAETIAVSILKQVMEEKVTPNNVDIAKVAPAYHLYTPQEVEAVIARL 237

HSP 2 Score: 45.0542 bits (105), Expect = 1.814e-74
Identity = 21/21 (100.00%), Postives = 21/21 (100.00%), Query Frame = 1 
Query:   13 SGLIADARTLVEHARVETQNH 75
            SGLIADARTLVEHARVETQNH
Sbjct:   79 SGLIADARTLVEHARVETQNH 99
[Close]
BLAST of EY757381 vs. ExPASy Swiss-Prot
Match: PSA5_DICDI (Proteasome subunit alpha type-5 OS=Dictyostelium discoideum GN=psmA5 PE=3 SV=1)

HSP 1 Score: 162.155 bits (409), Expect = 1.958e-45
Identity = 84/143 (58.74%), Postives = 105/143 (73.43%), Query Frame = 2 
Query:   80 FSYGEPMTVESTTQALCDLALRFGEG---DEESMSRPFGVSLLIAGSDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIARVAPTDH---LYTPSEVEAVINRL 490
            F+Y EPM VES  Q++CDLALRFGE     EE MSRPFGV+LLIAG DE GPSLYY+DPSGTF Q +AKAIG+GSEGA ++LQE+Y+K LT+ E + +AL+ LKQVMEE +T  NV++A +   D    +Y   E+  VI  L
Sbjct:   98 FNYNEPMGVESCVQSICDLALRFGENRNSGEERMSRPFGVALLIAGIDEKGPSLYYSDPSGTFTQFHAKAIGAGSEGAQTTLQEKYSKTLTIAECQKLALTTLKQVMEEAITTTNVELAVITKADQKFKIYNKEELATVIQTL 240

HSP 2 Score: 41.9726 bits (97), Expect = 1.958e-45
Identity = 16/21 (76.19%), Postives = 21/21 (100.00%), Query Frame = 1 
Query:   13 SGLIADARTLVEHARVETQNH 75
            SGL+ADART+++HAR+ETQNH
Sbjct:   76 SGLVADARTIIDHARIETQNH 96
[Close]
BLAST of EY757381 vs. ExPASy Swiss-Prot
Match: PSA5_SCHPO (Probable proteasome subunit alpha type-5 OS=Schizosaccharomyces pombe GN=SPAC323.02c PE=1 SV=1)

HSP 1 Score: 154.836 bits (390), Expect = 6.634e-43
Identity = 77/141 (54.61%), Postives = 105/141 (74.47%), Query Frame = 2 
Query:   80 FSYGEPMTVESTTQALCDLALRFGEGD---EESMSRPFGVSLLIAGSDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIARVAPTD--HLYTPSEVEAVINR 487
            F+Y EP  +ESTTQ++CDLALRFGEG+   E  MSRPFGV+LLIAG DE+GP LY+++PSGT+++  AKAIGSGSE A S L ++++KD+TL+EAE + L +L+QVMEEK+   NV +A+V      H+Y   E+   + R
Sbjct:  101 FTYDEPQGIESTTQSICDLALRFGEGEDGEERIMSRPFGVALLIAGIDEHGPQLYHSEPSGTYFRYEAKAIGSGSEPAKSELVKEFHKDMTLEEAEVLILKVLRQVMEEKLDSKNVQLAKVTAEGGFHIYNDEEMADAVAR 241

HSP 2 Score: 40.817 bits (94), Expect = 6.634e-43
Identity = 17/21 (80.95%), Postives = 20/21 (95.24%), Query Frame = 1 
Query:   13 SGLIADARTLVEHARVETQNH 75
            SGL ADART++EHARV+TQNH
Sbjct:   79 SGLTADARTIIEHARVQTQNH 99
[Close]
BLAST of EY757381 vs. ExPASy Swiss-Prot
Match: PSA5_MOUSE (Proteasome subunit alpha type-5 OS=Mus musculus GN=Psma5 PE=1 SV=1)

HSP 1 Score: 154.451 bits (389), Expect = 3.249e-42
Identity = 81/138 (58.70%), Postives = 103/138 (74.64%), Query Frame = 2 
Query:   80 FSYGEPMTVESTTQALCDLALRFGEGDEE--SMSRPFGVSLLIAGSDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIARVAPTD--HLYTPSEVEAVI 481
            F+Y E MTVES TQA+ +LAL+FGE D +  +MSRPFGV+LL  G DE GP L++ DPSGTF QC+A+AIGS SEGA SSLQE Y+K +TL+EA   +L ILKQVMEEK+   N+++A V P    H++T  E+E VI
Sbjct:  101 FTYNETMTVESVTQAVSNLALQFGEEDADPGAMSRPFGVALLFGGVDEKGPQLFHMDPSGTFVQCDARAIGSASEGAQSSLQEVYHKSMTLKEAIKSSLIILKQVMEEKLNATNIELATVQPGQNFHMFTKEELEEVI 238

HSP 2 Score: 38.891 bits (89), Expect = 3.249e-42
Identity = 17/21 (80.95%), Postives = 20/21 (95.24%), Query Frame = 1 
Query:   13 SGLIADARTLVEHARVETQNH 75
            SGLIADA+TL++ ARVETQNH
Sbjct:   79 SGLIADAKTLIDKARVETQNH 99
[Close]
BLAST of EY757381 vs. ExPASy Swiss-Prot
Match: PSA5_HUMAN (Proteasome subunit alpha type-5 OS=Homo sapiens GN=PSMA5 PE=1 SV=3)

HSP 1 Score: 154.451 bits (389), Expect = 3.249e-42
Identity = 81/138 (58.70%), Postives = 103/138 (74.64%), Query Frame = 2 
Query:   80 FSYGEPMTVESTTQALCDLALRFGEGDEE--SMSRPFGVSLLIAGSDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIARVAPTD--HLYTPSEVEAVI 481
            F+Y E MTVES TQA+ +LAL+FGE D +  +MSRPFGV+LL  G DE GP L++ DPSGTF QC+A+AIGS SEGA SSLQE Y+K +TL+EA   +L ILKQVMEEK+   N+++A V P    H++T  E+E VI
Sbjct:  101 FTYNETMTVESVTQAVSNLALQFGEEDADPGAMSRPFGVALLFGGVDEKGPQLFHMDPSGTFVQCDARAIGSASEGAQSSLQEVYHKSMTLKEAIKSSLIILKQVMEEKLNATNIELATVQPGQNFHMFTKEELEEVI 238

HSP 2 Score: 38.891 bits (89), Expect = 3.249e-42
Identity = 17/21 (80.95%), Postives = 20/21 (95.24%), Query Frame = 1 
Query:   13 SGLIADARTLVEHARVETQNH 75
            SGLIADA+TL++ ARVETQNH
Sbjct:   79 SGLIADAKTLIDKARVETQNH 99
[Close]
BLAST of EY757381 vs. ExPASy Swiss-Prot
Match: PSA5_BOVIN (Proteasome subunit alpha type-5 OS=Bos taurus GN=PSMA5 PE=1 SV=1)

HSP 1 Score: 154.451 bits (389), Expect = 3.249e-42
Identity = 81/138 (58.70%), Postives = 103/138 (74.64%), Query Frame = 2 
Query:   80 FSYGEPMTVESTTQALCDLALRFGEGDEE--SMSRPFGVSLLIAGSDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIARVAPTD--HLYTPSEVEAVI 481
            F+Y E MTVES TQA+ +LAL+FGE D +  +MSRPFGV+LL  G DE GP L++ DPSGTF QC+A+AIGS SEGA SSLQE Y+K +TL+EA   +L ILKQVMEEK+   N+++A V P    H++T  E+E VI
Sbjct:  101 FTYNETMTVESVTQAVSNLALQFGEEDADPGAMSRPFGVALLFGGVDEKGPQLFHMDPSGTFVQCDARAIGSASEGAQSSLQEVYHKSMTLKEAIKSSLIILKQVMEEKLNATNIELATVQPGQNFHMFTKEELEEVI 238

HSP 2 Score: 38.891 bits (89), Expect = 3.249e-42
Identity = 17/21 (80.95%), Postives = 20/21 (95.24%), Query Frame = 1 
Query:   13 SGLIADARTLVEHARVETQNH 75
            SGLIADA+TL++ ARVETQNH
Sbjct:   79 SGLIADAKTLIDKARVETQNH 99
[Close]
BLAST of EY757381 vs. ExPASy Swiss-Prot
Match: PSA5_RAT (Proteasome subunit alpha type-5 OS=Rattus norvegicus GN=Psma5 PE=2 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 7.189e-42
Identity = 81/138 (58.70%), Postives = 102/138 (73.91%), Query Frame = 2 
Query:   80 FSYGEPMTVESTTQALCDLALRFGEGDEE--SMSRPFGVSLLIAGSDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIARVAPTD--HLYTPSEVEAVI 481
            F+Y E MTVES TQA+ +LAL+FGE D +  +MSRPFGV+LL  G DE GP L++ DPSGTF QC+A+AIGS SEGA SSLQE Y+K  TL+EA   +L ILKQVMEEK+   N+++A V P    H++T  E+E VI
Sbjct:  101 FTYNETMTVESVTQAVSNLALQFGEEDADPGAMSRPFGVALLFGGVDEKGPQLFHMDPSGTFVQCDARAIGSASEGAQSSLQEVYHKSTTLKEAIKSSLIILKQVMEEKLNATNIELATVQPGQNFHMFTKEELEEVI 238

HSP 2 Score: 38.891 bits (89), Expect = 7.189e-42
Identity = 17/21 (80.95%), Postives = 20/21 (95.24%), Query Frame = 1 
Query:   13 SGLIADARTLVEHARVETQNH 75
            SGLIADA+TL++ ARVETQNH
Sbjct:   79 SGLIADAKTLIDKARVETQNH 99
The following BLAST results are available for this feature:
BLAST of EY757381 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 110
Match NameE-valueIdentityDescription
PSA5_SOYBN1.166e-7695.62Proteasome subunit alpha type-5 OS=Glycine max GN=... [more]
PSA5_ORYSJ2.826e-7593.43Proteasome subunit alpha type-5 OS=Oryza sativa su... [more]
PSA5B_ARATH4.808e-7592.70Proteasome subunit alpha type-5-B OS=Arabidopsis t... [more]
PSA5A_ARATH1.814e-7492.70Proteasome subunit alpha type-5-A OS=Arabidopsis t... [more]
PSA5_DICDI1.958e-4558.74Proteasome subunit alpha type-5 OS=Dictyostelium d... [more]
PSA5_SCHPO6.634e-4354.61Probable proteasome subunit alpha type-5 OS=Schizo... [more]
PSA5_MOUSE3.249e-4258.70Proteasome subunit alpha type-5 OS=Mus musculus GN... [more]
PSA5_HUMAN3.249e-4258.70Proteasome subunit alpha type-5 OS=Homo sapiens GN... [more]
PSA5_BOVIN3.249e-4258.70Proteasome subunit alpha type-5 OS=Bos taurus GN=P... [more]
PSA5_RAT7.189e-4258.70Proteasome subunit alpha type-5 OS=Rattus norvegic... [more]

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Properties
Property NameValue
Genbank descriptionCS13-C1-001-022-F11-CT.F Sweet orange leaf, field plant B Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY757381 ID=EY757381; Name=EY757381; organism=Citrus sinensis; type=EST; length=788bp
CCACGCGTCCGGAGTGGATTGATTGCTGATGCTCGTACACTTGTTGAACA
TGCAAGGGTTGAAACTCAGAATCATATGATTCTCATATGGTGAGCCAATG
ACCGTAGAATCTACTACACAAGCACTCTGTGACCTGGCGCTACGCTTTGG
TGAAGGCGATGAAGAATCCATGTCTCGACCATTTGGAGTATCCCTTCTTA
TTGCTGGATCCGACGAGAATGGACCTAGCCTGTACTATACAGATCCATCT
GGCACATTCTGGCAATGCAATGCAAAGGCTATTGGTTCAGGTTCAGAAGG
AGCTGACAGTTCTCTCCAAGAGCAATATAACAAGGATTTAACCCTTCAGG
AAGCAGAGACAATTGCTCTATCAATTTTGAAGCAAGTTATGGAAGAAAAG
GTCACTCCAAACAATGTTGATATCGCAAGGGTAGCTCCAACGGATCATCT
CTATACCCCTTCTGAGGTGGAGGCTGTCATCAATCGCCTATGAGTATCCA
GAGAAAATCATCTTTAAAATTTCTTCCCTGGCTTCAGTGGTGTTTCTTCA
GTTCTTTGCAGTGCAAGTGCACTTCGTAGTTTGTTTGTTAGGGGAAACAA
TTATATTTGCTTATGATTGGGATTGCACCCATTTGAACCTTTCTGCTTTG
GTGCTGGGTTTGAGCTGCCTATCATAACCCCGATATCTGTGTCCGTGCAG
AACTGAAGTACTTCTCAGTTGTATGAACGATATGAACCTAATGGAAACTT
AGATTAAAATCTTGTTTTTAAGTTAAATAAAAAAAAAA
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