CX294316

Overview
NameCX294316
Unique NameCX294316
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length508
Libraries
Library NameType
FlavSen1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig9261 contig Ccv1_Contig9261:21..530. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CX294316 vs. ExPASy Swiss-Prot
Match: CYB51_ARATH (Cytochrome b5 isoform 1 OS=Arabidopsis thaliana GN=At5g53560 PE=2 SV=2)

HSP 1 Score: 118.627 bits (296), Expect = 7.588e-39
Identity = 53/64 (82.81%), Postives = 59/64 (92.19%), Query Frame = 2 
Query:  197 MASDPKIHQFEEVATHNKTKDCWLIISGKVYDVSSFMDDHPGGDEVLISATGKDATNDFEDVGH 388
            M+SD K+  FEEV+ HNKTKDCWLIISGKVYDV+ FMDDHPGGDEVL+S+TGKDATNDFEDVGH
Sbjct:    1 MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDVGH 64

HSP 2 Score: 61.6178 bits (148), Expect = 7.588e-39
Identity = 26/40 (65.00%), Postives = 34/40 (85.00%), Query Frame = 3 
Query:  387 TDSAREMMDKYYIGDIDPSTVPRKRAYIPLEQPAYNQDKT 506
            +D+AR+MMDKY+IG+ID S+VP  R Y+  +QPAYNQDKT
Sbjct:   65 SDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKT 104
[Close]
BLAST of CX294316 vs. ExPASy Swiss-Prot
Match: CYB5_BRAOB (Cytochrome b5 OS=Brassica oleracea var. botrytis GN=CYB5 PE=1 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 1.288e-38
Identity = 53/64 (82.81%), Postives = 59/64 (92.19%), Query Frame = 2 
Query:  197 MASDPKIHQFEEVATHNKTKDCWLIISGKVYDVSSFMDDHPGGDEVLISATGKDATNDFEDVGH 388
            MAS+ K+  FEEV+ HNKTKDCWLIISGKVYDV+ FMDDHPGGDEVL+S+TGKDATNDFEDVGH
Sbjct:    1 MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDVGH 64

HSP 2 Score: 61.6178 bits (148), Expect = 1.288e-38
Identity = 27/40 (67.50%), Postives = 33/40 (82.50%), Query Frame = 3 
Query:  387 TDSAREMMDKYYIGDIDPSTVPRKRAYIPLEQPAYNQDKT 506
            +D+AR+MM+KYYIG+ID STVP  R Y+   QPAYNQDKT
Sbjct:   65 SDTARDMMEKYYIGEIDSSTVPATRTYVAPVQPAYNQDKT 104
[Close]
BLAST of CX294316 vs. ExPASy Swiss-Prot
Match: CYB5_TOBAC (Cytochrome b5 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 1.117e-33
Identity = 45/64 (70.31%), Postives = 53/64 (82.81%), Query Frame = 2 
Query:  197 MASDPKIHQFEEVATHNKTKDCWLIISGKVYDVSSFMDDHPGGDEVLISATGKDATNDFEDVGH 388
            M  + K+    EV+ HN  KDCWL+ISGKVYDV+ F+DDHPGGDEVL+SATGKDAT+DFEDVGH
Sbjct:    1 MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFEDVGH 64

HSP 2 Score: 57.3806 bits (137), Expect = 1.117e-33
Identity = 24/40 (60.00%), Postives = 30/40 (75.00%), Query Frame = 3 
Query:  387 TDSAREMMDKYYIGDIDPSTVPRKRAYIPLEQPAYNQDKT 506
            + SAR M+D+YY+GDID +T+P K  Y P  QP YNQDKT
Sbjct:   65 SSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYNQDKT 104
[Close]
BLAST of CX294316 vs. ExPASy Swiss-Prot
Match: CYB52_ARATH (Probable cytochrome b5 isoform 2 OS=Arabidopsis thaliana GN=At2g32720 PE=1 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 1.569e-32
Identity = 39/64 (60.94%), Postives = 53/64 (82.81%), Query Frame = 2 
Query:  197 MASDPKIHQFEEVATHNKTKDCWLIISGKVYDVSSFMDDHPGGDEVLISATGKDATNDFEDVGH 388
            M  + KI    EV+ HN+  DCW++I+GKVY+V+ F++DHPGGD+VL+S+TGKDAT+DFEDVGH
Sbjct:    1 MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGH 64

HSP 2 Score: 62.3882 bits (150), Expect = 1.569e-32
Identity = 25/40 (62.50%), Postives = 35/40 (87.50%), Query Frame = 3 
Query:  387 TDSAREMMDKYYIGDIDPSTVPRKRAYIPLEQPAYNQDKT 506
            ++SAREMM++YY+G+IDP+T+P+K  Y P +QP YNQDKT
Sbjct:   65 SESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKT 104
[Close]
BLAST of CX294316 vs. ExPASy Swiss-Prot
Match: CYB5_ORYSJ (Cytochrome b5 OS=Oryza sativa subsp. japonica GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 99.3673 bits (246), Expect = 2.660e-32
Identity = 42/59 (71.19%), Postives = 51/59 (86.44%), Query Frame = 2 
Query:  212 KIHQFEEVATHNKTKDCWLIISGKVYDVSSFMDDHPGGDEVLISATGKDATNDFEDVGH 388
            K++  EEVA HN   DCWLII GKVY+VS F++DHPGGD+VL+S+TGKDAT+DFEDVGH
Sbjct:    7 KVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFEDVGH 65

HSP 2 Score: 58.9214 bits (141), Expect = 2.660e-32
Identity = 24/38 (63.16%), Postives = 31/38 (81.58%), Query Frame = 3 
Query:  393 SAREMMDKYYIGDIDPSTVPRKRAYIPLEQPAYNQDKT 506
            +AR MMD+YY+GDID ST+P +  Y+P +QP YNQDKT
Sbjct:   68 TARAMMDEYYVGDIDTSTIPARTKYVPPKQPHYNQDKT 105
[Close]
BLAST of CX294316 vs. ExPASy Swiss-Prot
Match: CYB5S_TOBAC (Cytochrome b5, seed isoform OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 5.880e-32
Identity = 42/64 (65.62%), Postives = 51/64 (79.69%), Query Frame = 2 
Query:  197 MASDPKIHQFEEVATHNKTKDCWLIISGKVYDVSSFMDDHPGGDEVLISATGKDATNDFEDVGH 388
            M    K+    EV+ HN  KDCWLIISGKVY+V+ F++DHPGG EVL+SATGKDAT+DFED+GH
Sbjct:    1 MGGQSKVFTLAEVSNHNNAKDCWLIISGKVYNVTKFLEDHPGGGEVLLSATGKDATDDFEDIGH 64

HSP 2 Score: 58.151 bits (139), Expect = 5.880e-32
Identity = 25/40 (62.50%), Postives = 31/40 (77.50%), Query Frame = 3 
Query:  387 TDSAREMMDKYYIGDIDPSTVPRKRAYIPLEQPAYNQDKT 506
            + SAR M+D+YY+GDID ST+P K  Y P +QP YNQDKT
Sbjct:   65 SSSARAMLDEYYVGDIDSSTIPTKVKYTPPKQPHYNQDKT 104
[Close]
BLAST of CX294316 vs. ExPASy Swiss-Prot
Match: CYB5_BOROF (Cytochrome b5 OS=Borago officinalis PE=2 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 8.257e-31
Identity = 43/59 (72.88%), Postives = 52/59 (88.14%), Query Frame = 2 
Query:  212 KIHQFEEVATHNKTKDCWLIISGKVYDVSSFMDDHPGGDEVLISATGKDATNDFEDVGH 388
            KI    EVA HN +KDCWLII+GKVYDV+ F++DHPGGD+VL+SATGKDAT+DFED+GH
Sbjct:    3 KIFTLAEVAQHNNSKDCWLIINGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFEDIGH 61

HSP 2 Score: 51.9878 bits (123), Expect = 8.257e-31
Identity = 21/40 (52.50%), Postives = 30/40 (75.00%), Query Frame = 3 
Query:  387 TDSAREMMDKYYIGDIDPSTVPRKRAYIPLEQPAYNQDKT 506
            + SA+ M+D+YY+GDID S++P +  Y P +QP YN DKT
Sbjct:   62 SSSAKAMLDEYYVGDIDSSSIPSQVKYTPPKQPLYNPDKT 101
[Close]
BLAST of CX294316 vs. ExPASy Swiss-Prot
Match: CYB5_CUSRE (Cytochrome b5 OS=Cuscuta reflexa PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 1.244e-28
Identity = 36/59 (61.02%), Postives = 49/59 (83.05%), Query Frame = 2 
Query:  212 KIHQFEEVATHNKTKDCWLIISGKVYDVSSFMDDHPGGDEVLISATGKDATNDFEDVGH 388
            K++   EV+ H++  DCWL+I GKVYDV+ F+DDHPGG +VL+S+T KDAT+DFED+GH
Sbjct:    5 KVYSLAEVSEHSQPNDCWLVIGGKVYDVTKFLDDHPGGADVLLSSTAKDATDDFEDIGH 63

HSP 2 Score: 54.6842 bits (130), Expect = 1.244e-28
Identity = 24/40 (60.00%), Postives = 30/40 (75.00%), Query Frame = 3 
Query:  387 TDSAREMMDKYYIGDIDPSTVPRKRAYIPLEQPAYNQDKT 506
            + SAR MMD+  +GDID ST+P K +Y P +QP YNQDKT
Sbjct:   64 SSSARAMMDEMCVGDIDSSTIPTKTSYTPPKQPLYNQDKT 103
[Close]
BLAST of CX294316 vs. ExPASy Swiss-Prot
Match: CYB5B_PONAB (Cytochrome b5 type B OS=Pongo abelii GN=CYB5B PE=2 SV=2)

HSP 1 Score: 76.2554 bits (186), Expect = 7.421e-17
Identity = 31/66 (46.97%), Postives = 47/66 (71.21%), Query Frame = 2 
Query:  191 EQMASDPKIHQFEEVATHNKTKDCWLIISGKVYDVSSFMDDHPGGDEVLISATGKDATNDFEDVGH 388
            +++ +    ++ EEVA  N  K+ WL+I G+VYDV+ F+++HPGG+EVL+   G DA+  FEDVGH
Sbjct:   14 QEVETSVTYYRMEEVAKRNSLKELWLVIHGRVYDVTRFLNEHPGGEEVLLEQAGVDASESFEDVGH 79

HSP 2 Score: 30.0314 bits (66), Expect = 7.421e-17
Identity = 12/19 (63.16%), Postives = 15/19 (78.95%), Query Frame = 3 
Query:  387 TDSAREMMDKYYIGDIDPS 443
            +  AREM+ +YYIGDI PS
Sbjct:   80 SSDAREMLKQYYIGDIHPS 98
[Close]
BLAST of CX294316 vs. ExPASy Swiss-Prot
Match: CYB5B_MOUSE (Cytochrome b5 type B OS=Mus musculus GN=Cyb5b PE=1 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 7.421e-17
Identity = 31/64 (48.44%), Postives = 46/64 (71.88%), Query Frame = 2 
Query:  203 SDPKI--HQFEEVATHNKTKDCWLIISGKVYDVSSFMDDHPGGDEVLISATGKDATNDFEDVGH 388
            S+P +  ++ EEVA  N  ++ W++I G+VYD++ F+ +HPGG+EVL+   G DAT  FEDVGH
Sbjct:   16 SEPSVTYYRLEEVAKRNSAEETWMVIHGRVYDITRFLSEHPGGEEVLLEQAGADATESFEDVGH 79

HSP 2 Score: 30.0314 bits (66), Expect = 7.421e-17
Identity = 12/21 (57.14%), Postives = 16/21 (76.19%), Query Frame = 3 
Query:  396 AREMMDKYYIGDIDPSTVPRK 458
            AREM+ +YYIGD+ PS +  K
Sbjct:   83 AREMLKQYYIGDVHPSDLKPK 103
The following BLAST results are available for this feature:
BLAST of CX294316 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 31
Match NameE-valueIdentityDescription
CYB51_ARATH7.588e-3982.81Cytochrome b5 isoform 1 OS=Arabidopsis thaliana GN... [more]
CYB5_BRAOB1.288e-3882.81Cytochrome b5 OS=Brassica oleracea var. botrytis G... [more]
CYB5_TOBAC1.117e-3370.31Cytochrome b5 OS=Nicotiana tabacum PE=2 SV=1[more]
CYB52_ARATH1.569e-3260.94Probable cytochrome b5 isoform 2 OS=Arabidopsis th... [more]
CYB5_ORYSJ2.660e-3271.19Cytochrome b5 OS=Oryza sativa subsp. japonica GN=O... [more]
CYB5S_TOBAC5.880e-3265.63Cytochrome b5, seed isoform OS=Nicotiana tabacum P... [more]
CYB5_BOROF8.257e-3172.88Cytochrome b5 OS=Borago officinalis PE=2 SV=1[more]
CYB5_CUSRE1.244e-2861.02Cytochrome b5 OS=Cuscuta reflexa PE=2 SV=1[more]
CYB5B_PONAB7.421e-1746.97Cytochrome b5 type B OS=Pongo abelii GN=CYB5B PE=2... [more]
CYB5B_MOUSE7.421e-1748.44Cytochrome b5 type B OS=Mus musculus GN=Cyb5b PE=1... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionC05015A12SK FlavSen1 Citrus clementina cDNA clone C05015A12, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX294316 ID=CX294316; Name=CX294316; organism=Citrus clementina; type=EST; length=508bp
ATTCCTCACTTTGTTTGATTTCAAAATCTACTTCTTTAGAGTTTAAACAT
AAAAGGGTTTCTTTATTTGCTGTTGTTTTGATCAAAAAGTTCCCTTTTTG
GTTGGTGTTTGGGTCGATTCTACATTTTTACTTTATTTTTTTGTGGGGTT
TTTTTGAATTTGTTGAGGTTTTGTGTTTAGCTGCAGTAGTGAACAAATGG
CCTCAGATCCAAAGATTCACCAGTTCGAAGAGGTCGCCACGCACAACAAA
ACTAAGGATTGCTGGCTTATTATTTCTGGCAAGGTGTATGATGTGTCCTC
GTTCATGGATGATCACCCTGGAGGAGATGAGGTTTTGATTTCTGCAACTG
GGAAAGATGCAACAAATGACTTTGAAGATGTGGGTCACTGACTCCGCTAG
GGAGATGATGGACAAGTACTATATTGGTGACATTGATCCCTCAACTGTTC
CTCGGAAACGTGCATATATTCCACTGGAGCAACCTGCTTACAATCAAGAC
AAGACCCC
back to top