DY262577

Overview
NameDY262577
Unique NameDY262577
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length1203
Libraries
Library NameType
CitNFLcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
DY262577_ssr23 microsatellite DY262577_ssr23:23..34. BLAST: Citrus ESTs to Prunus persica proteins V1
DY262577_ssr151 microsatellite DY262577_ssr151:151..162. BLAST: Citrus ESTs to Prunus persica proteins V1
Ccv1_Contig2329 contig Ccv1_Contig2329:1..1203. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of DY262577 vs. ExPASy Swiss-Prot
Match: 1A1D_PYRHO (Putative 1-aminocyclopropane-1-carboxylate deaminase OS=Pyrococcus horikoshii GN=PH0054 PE=1 SV=2)

HSP 1 Score: 124.79 bits (312), Expect = 1.057e-30
Identity = 66/164 (40.24%), Postives = 104/164 (63.41%), Query Frame = 2 
Query:  317 TPIHKWNLPNLPHN--TEVWLKRDDLSGMQLSGNKVRKLEFLMADAVAQGADCIITIGGIQSNHCRAAAVAAKYLNLDCYLILRTSKVLVDQDPGLTGNLLVERLVGAHIELISKEEYSKIGSVTLTNILKEKLLKEGRRPYVIPVGGSNSIGTWGYIEAIKEI 802
            TPI    LPN+      +V++KRDDL+G+ + GNK+RKLE+L+ DA+++GAD +IT+G + SNH     +AAK L LD  L+LR  +        L GN L+++++G    +   ++  ++  +     + E+L +EGR+PYVIP GG++ IGT GY+ A+ EI
Sbjct:   22 TPIQY--LPNISREIGADVYIKRDDLTGLGIGGNKIRKLEYLLGDALSKGADVVITVGAVHSNHAFVTGLAAKKLGLDAILVLRGKE-------ELKGNYLLDKIMGIETRVYDAKDSFEL--MKYAEEIAEELKREGRKPYVIPPGGASPIGTLGYVRAVGEI 174

HSP 2 Score: 31.187 bits (69), Expect = 1.057e-30
Identity = 14/16 (87.50%), Postives = 15/16 (93.75%), Query Frame = 1 
Query:  850 VVACGSGGTIAGLSLG 897
            VVA GSGGT+AGLSLG
Sbjct:  186 VVAAGSGGTLAGLSLG 201
[Close]
BLAST of DY262577 vs. ExPASy Swiss-Prot
Match: 1A1D_THEMA (Putative 1-aminocyclopropane-1-carboxylate deaminase OS=Thermotoga maritima GN=TM_0225 PE=3 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 5.547e-29
Identity = 66/167 (39.52%), Postives = 102/167 (61.08%), Query Frame = 2 
Query:  314 PTPIHKWNLPNLPHNTEVWLKRDDLSGMQLSGNKVRKLEFLMADAVAQGADCIITIGGIQSNHCRAAAVAAKYLNLDCYLILRTSKVLVDQDPGLTGNLLVERLVGAHIELISKEEYSKIGSVTLTNILKEKLLKEGRRPYVIPVGGSNSIGTWGYIEAIKEIEQXL 814
            PTP+      +  +   +++KRDDL+ +  SGNK+RKLE+L+ +A+ +GA  + T GG+QSNH RA A  ++   L   L LR  + +      L GNLL++ L+GA I  +S EEY +I  +   ++ K+   K+G + YVIP GGSNS+G +GY  A+ E++  L
Sbjct:    9 PTPVQFLKRLSEKYGFNIYVKRDDLTELVGSGNKIRKLEYLLWEALKKGATTVFTCGGLQSNHARATAYVSRRYGLKPVLFLRKGEKV------LNGNLLLDILLGAEIVEVSPEEYERIDEI--FDVHKKMREKKGEKVYVIPEGGSNSLGAFGYFNAVLEMKDQL 167

HSP 2 Score: 30.0314 bits (66), Expect = 5.547e-29
Identity = 20/50 (40.00%), Postives = 26/50 (52.00%), Query Frame = 1 
Query:  850 VVACGSGGTIAGLSLG-SWLGTLKAKVHAFSVCDDPDYFYDYTQGLLDGL 996
            V A GSGGTIAGLS G S+L      V       + DYF    + ++ G+
Sbjct:  176 VCAVGSGGTIAGLSAGISFLEYHVPVVGVNVTTKNSDYFVGKVKRIISGM 225
[Close]
BLAST of DY262577 vs. ExPASy Swiss-Prot
Match: 1A1D_PYRAB (Putative 1-aminocyclopropane-1-carboxylate deaminase OS=Pyrococcus abyssi GN=PYRAB00630 PE=3 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 1.314e-27
Identity = 60/147 (40.82%), Postives = 95/147 (64.63%), Query Frame = 2 
Query:  362 EVWLKRDDLSGMQLSGNKVRKLEFLMADAVAQGADCIITIGGIQSNHCRAAAVAAKYLNLDCYLILRTSKVLVDQDPGLTGNLLVERLVGAHIELISKEEYSKIGSVTLTNILKEKLLKEGRRPYVIPVGGSNSIGTWGYIEAIKEI 802
            +V++KRDDL+G+ + GNK+RKLEFL+ DA+++G D +ITIG + SNH    A+AAK L L   LILR  +V       L GN L+++L+G    +   +   ++  + +   + E+L  EG++PY+IP GG++ +GT GYI  + E+
Sbjct:   37 DVYVKRDDLTGLGIGGNKIRKLEFLLGDALSRGCDTVITIGAVHSNHAFVTALAAKKLGLGAVLILRGEEV-------LKGNYLLDKLMGIETRIYEADNSWEL--MKVAEEVAEELKGEGKKPYIIPPGGASPVGTLGYIRGVGEL 174

HSP 2 Score: 26.5646 bits (57), Expect = 1.314e-27
Identity = 21/68 (30.88%), Postives = 32/68 (47.06%), Query Frame = 1 
Query:  850 VVACGSGGTIAGLSLGSWLGTLKAKVHAFSVCDDPDYFYDYTQGLLDGLN--AGIDSHDLVNIQNPKV 1047
            V A GSGGT AGL LGS +   +  V    V    +   +  + L++      GI+    V +Q P++
Sbjct:  189 VDAVGSGGTYAGLLLGSAIVNAEWSVVGIDVSSATEKAKERVKNLVEKTKELLGIN----VKVQEPRI 252
[Close]
BLAST of DY262577 vs. ExPASy Swiss-Prot
Match: 1A1D_PYRFU (Putative 1-aminocyclopropane-1-carboxylate deaminase OS=Pyrococcus furiosus GN=PF0010 PE=3 SV=2)

HSP 1 Score: 114.775 bits (286), Expect = 2.227e-27
Identity = 64/166 (38.55%), Postives = 100/166 (60.24%), Query Frame = 2 
Query:  317 TPIHKWNLPNLPH--NTEVWLKRDDLSGMQLSGNKVRKLEFLMADAVAQGADCIITIGGIQSNHCRAAAVAAKYLNLDCYLILRTSKVLVDQDPGLTGNLLVERLVGAHIELISKEEYSKIGSVTLTNILKEKLLKEGRRPYVIPVGGSNSIGTWGYIEAIKEIEQ 808
            TPI    LPN+      ++++KRDDL+G+ + GNK+RKLE+L+ DA+ + AD IIT+G + SNH     +AAK L  D  L+LR  +        L GN L+++++G  IE    E       +     + ++L ++GR+PY+IPVGG++ +GT GY+ A  EI +
Sbjct:   22 TPIQY--LPNISKLVGADIYVKRDDLTGLGIGGNKIRKLEYLLGDAIIRKADVIITVGAVHSNHAFVTGLAAKKLGFDVVLVLRGKE-------ELRGNYLLDKIMG--IETRVYEAKDSFELMKYAEEVAKELEEKGRKPYIIPVGGASPVGTLGYVRASGEIAE 176

HSP 2 Score: 30.0314 bits (66), Expect = 2.227e-27
Identity = 16/31 (51.61%), Postives = 21/31 (67.74%), Query Frame = 1 
Query:  850 VVACGSGGTIAGLSLGSWLGTLKAKVHAFSV 942
            VVA GSGGT+AGLS+G  L  L+ +  A  +
Sbjct:  189 VVATGSGGTLAGLSVG--LAILRKETRAIGM 217
[Close]
BLAST of DY262577 vs. ExPASy Swiss-Prot
Match: DCYD_SALTY (D-cysteine desulfhydrase OS=Salmonella typhimurium GN=dcyD PE=3 SV=3)

HSP 1 Score: 113.235 bits (282), Expect = 3.335e-25
Identity = 74/189 (39.15%), Postives = 102/189 (53.97%), Query Frame = 2 
Query:  269 HLTPIPSHVFSLGHFPTPIHKWNLPNLPH--NTEVWLKRDDLSGMQLSGNKVRKLEFLMADAVAQGADCIITIGGIQSNHCRAAAVAAKYLNLDCYLILRTSKVLVDQDPGLTGNLLVERLVGAHIELISKEEYSKIGSVTLTNILKEKLLKEGRRPYVIPVGGSNSIGTWGYIEAIKEIEQXLQTGTG 829
            HLT  P   F +G  PTP+    LP L      E+++KRDD++ + + GNK+RKLEFL+ADA+ +GAD +IT G IQSNH R  A  A  L L C  +L        ++    GN L+  L    IE+            TL      ++  +G RPYVIPVGGS+++G  GY+E+  EI Q  +   G
Sbjct:    5 HLTRFPRLEF-IGA-PTPLEY--LPRLSDYLGREIYIKRDDVTPIAMGGNKLRKLEFLVADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENPIGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPDAQLQTLAT----RIEAQGFRPYVIPVGGSSALGAMGYVESALEIAQQCEEVVG 185

HSP 2 Score: 24.2534 bits (51), Expect = 3.335e-25
Identity = 11/16 (68.75%), Postives = 13/16 (81.25%), Query Frame = 1 
Query:  850 VVACGSGGTIAGLSLG 897
            VVA GS GT AGL++G
Sbjct:  190 VVASGSAGTHAGLAVG 205
[Close]
BLAST of DY262577 vs. ExPASy Swiss-Prot
Match: DCYD_SALSV (D-cysteine desulfhydrase OS=Salmonella schwarzengrund (strain CVM19633) GN=dcyD PE=3 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 3.335e-25
Identity = 73/189 (38.62%), Postives = 100/189 (52.91%), Query Frame = 2 
Query:  269 HLTPIPSHVFSLGHFPTPIHKWNLPNLPH--NTEVWLKRDDLSGMQLSGNKVRKLEFLMADAVAQGADCIITIGGIQSNHCRAAAVAAKYLNLDCYLILRTSKVLVDQDPGLTGNLLVERLVGAHIELISKEEYSKIGSVTLTNILKEKLLKEGRRPYVIPVGGSNSIGTWGYIEAIKEIEQXLQTGTG 829
            HLT  P     L   PTP+    LP L      E+++KRDD++ + + GNK+RKLEFL+ADA+ +GAD +IT G IQSNH R  A  A  L L C  +L        ++    GN L+  L    IE+            TL      ++  +G RPYVIPVGGS+++G  GY+E+  EI Q  +   G
Sbjct:    5 HLTRFPR--LELIGAPTPLEY--LPRLSDYLGREIYIKRDDVTPIAMGGNKLRKLEFLVADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENPIGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPDAQLQTLAT----RIEAQGFRPYVIPVGGSSALGAMGYVESALEIAQQCEEVVG 185

HSP 2 Score: 24.2534 bits (51), Expect = 3.335e-25
Identity = 11/16 (68.75%), Postives = 13/16 (81.25%), Query Frame = 1 
Query:  850 VVACGSGGTIAGLSLG 897
            VVA GS GT AGL++G
Sbjct:  190 VVASGSAGTHAGLAVG 205
[Close]
BLAST of DY262577 vs. ExPASy Swiss-Prot
Match: DCYD_SALPB (D-cysteine desulfhydrase OS=Salmonella paratyphi B (strain ATCC BAA-1250 / SPB7) GN=dcyD PE=3 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 3.335e-25
Identity = 73/189 (38.62%), Postives = 100/189 (52.91%), Query Frame = 2 
Query:  269 HLTPIPSHVFSLGHFPTPIHKWNLPNLPH--NTEVWLKRDDLSGMQLSGNKVRKLEFLMADAVAQGADCIITIGGIQSNHCRAAAVAAKYLNLDCYLILRTSKVLVDQDPGLTGNLLVERLVGAHIELISKEEYSKIGSVTLTNILKEKLLKEGRRPYVIPVGGSNSIGTWGYIEAIKEIEQXLQTGTG 829
            HLT  P     L   PTP+    LP L      E+++KRDD++ + + GNK+RKLEFL+ADA+ +GAD +IT G IQSNH R  A  A  L L C  +L        ++    GN L+  L    IE+            TL      ++  +G RPYVIPVGGS+++G  GY+E+  EI Q  +   G
Sbjct:    5 HLTRFPR--LELIGAPTPLEY--LPRLSDYLGREIYIKRDDVTPIAMGGNKLRKLEFLVADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENPIGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPDAQLQTLAT----RIEAQGFRPYVIPVGGSSALGAMGYVESALEIAQQCEEVVG 185

HSP 2 Score: 24.2534 bits (51), Expect = 3.335e-25
Identity = 11/16 (68.75%), Postives = 13/16 (81.25%), Query Frame = 1 
Query:  850 VVACGSGGTIAGLSLG 897
            VVA GS GT AGL++G
Sbjct:  190 VVASGSAGTHAGLAVG 205
[Close]
BLAST of DY262577 vs. ExPASy Swiss-Prot
Match: DCYD_SALNS (D-cysteine desulfhydrase OS=Salmonella newport (strain SL254) GN=dcyD PE=3 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 3.335e-25
Identity = 73/189 (38.62%), Postives = 100/189 (52.91%), Query Frame = 2 
Query:  269 HLTPIPSHVFSLGHFPTPIHKWNLPNLPH--NTEVWLKRDDLSGMQLSGNKVRKLEFLMADAVAQGADCIITIGGIQSNHCRAAAVAAKYLNLDCYLILRTSKVLVDQDPGLTGNLLVERLVGAHIELISKEEYSKIGSVTLTNILKEKLLKEGRRPYVIPVGGSNSIGTWGYIEAIKEIEQXLQTGTG 829
            HLT  P     L   PTP+    LP L      E+++KRDD++ + + GNK+RKLEFL+ADA+ +GAD +IT G IQSNH R  A  A  L L C  +L        ++    GN L+  L    IE+            TL      ++  +G RPYVIPVGGS+++G  GY+E+  EI Q  +   G
Sbjct:    5 HLTRFPR--LELIGAPTPLEY--LPRLSDYLGREIYIKRDDVTPIAMGGNKLRKLEFLVADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENPIGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPDAQLQTLAT----RIEAQGFRPYVIPVGGSSALGAMGYVESALEIAQQCEEVVG 185

HSP 2 Score: 24.2534 bits (51), Expect = 3.335e-25
Identity = 11/16 (68.75%), Postives = 13/16 (81.25%), Query Frame = 1 
Query:  850 VVACGSGGTIAGLSLG 897
            VVA GS GT AGL++G
Sbjct:  190 VVASGSAGTHAGLAVG 205
[Close]
BLAST of DY262577 vs. ExPASy Swiss-Prot
Match: DCYD_SALHS (D-cysteine desulfhydrase OS=Salmonella heidelberg (strain SL476) GN=dcyD PE=3 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 3.335e-25
Identity = 73/189 (38.62%), Postives = 100/189 (52.91%), Query Frame = 2 
Query:  269 HLTPIPSHVFSLGHFPTPIHKWNLPNLPH--NTEVWLKRDDLSGMQLSGNKVRKLEFLMADAVAQGADCIITIGGIQSNHCRAAAVAAKYLNLDCYLILRTSKVLVDQDPGLTGNLLVERLVGAHIELISKEEYSKIGSVTLTNILKEKLLKEGRRPYVIPVGGSNSIGTWGYIEAIKEIEQXLQTGTG 829
            HLT  P     L   PTP+    LP L      E+++KRDD++ + + GNK+RKLEFL+ADA+ +GAD +IT G IQSNH R  A  A  L L C  +L        ++    GN L+  L    IE+            TL      ++  +G RPYVIPVGGS+++G  GY+E+  EI Q  +   G
Sbjct:    5 HLTRFPR--LELIGAPTPLEY--LPRLSDYLGREIYIKRDDVTPIAMGGNKLRKLEFLVADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENPIGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPDAQLQTLAT----RIEAQGFRPYVIPVGGSSALGAMGYVESALEIAQQCEEVVG 185

HSP 2 Score: 24.2534 bits (51), Expect = 3.335e-25
Identity = 11/16 (68.75%), Postives = 13/16 (81.25%), Query Frame = 1 
Query:  850 VVACGSGGTIAGLSLG 897
            VVA GS GT AGL++G
Sbjct:  190 VVASGSAGTHAGLAVG 205
[Close]
BLAST of DY262577 vs. ExPASy Swiss-Prot
Match: DCYD_SALG2 (D-cysteine desulfhydrase OS=Salmonella gallinarum (strain 287/91 / NCTC 13346) GN=dcyD PE=3 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 3.335e-25
Identity = 73/189 (38.62%), Postives = 100/189 (52.91%), Query Frame = 2 
Query:  269 HLTPIPSHVFSLGHFPTPIHKWNLPNLPH--NTEVWLKRDDLSGMQLSGNKVRKLEFLMADAVAQGADCIITIGGIQSNHCRAAAVAAKYLNLDCYLILRTSKVLVDQDPGLTGNLLVERLVGAHIELISKEEYSKIGSVTLTNILKEKLLKEGRRPYVIPVGGSNSIGTWGYIEAIKEIEQXLQTGTG 829
            HLT  P     L   PTP+    LP L      E+++KRDD++ + + GNK+RKLEFL+ADA+ +GAD +IT G IQSNH R  A  A  L L C  +L        ++    GN L+  L    IE+            TL      ++  +G RPYVIPVGGS+++G  GY+E+  EI Q  +   G
Sbjct:    5 HLTRFPR--LELIGAPTPLEY--LPRLSDYLGREIYIKRDDVTPIAMGGNKLRKLEFLVADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENPIGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPDAQLQTLAT----RIEAQGFRPYVIPVGGSSALGAMGYVESALEIAQQCEEVVG 185

HSP 2 Score: 24.2534 bits (51), Expect = 3.335e-25
Identity = 11/16 (68.75%), Postives = 13/16 (81.25%), Query Frame = 1 
Query:  850 VVACGSGGTIAGLSLG 897
            VVA GS GT AGL++G
Sbjct:  190 VVASGSAGTHAGLAVG 205
The following BLAST results are available for this feature:
BLAST of DY262577 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 98
Match NameE-valueIdentityDescription
1A1D_PYRHO1.057e-3040.24Putative 1-aminocyclopropane-1-carboxylate deamina... [more]
1A1D_THEMA5.547e-2939.52Putative 1-aminocyclopropane-1-carboxylate deamina... [more]
1A1D_PYRAB1.314e-2740.82Putative 1-aminocyclopropane-1-carboxylate deamina... [more]
1A1D_PYRFU2.227e-2738.55Putative 1-aminocyclopropane-1-carboxylate deamina... [more]
DCYD_SALTY3.335e-2539.15D-cysteine desulfhydrase OS=Salmonella typhimurium... [more]
DCYD_SALSV3.335e-2538.62D-cysteine desulfhydrase OS=Salmonella schwarzengr... [more]
DCYD_SALPB3.335e-2538.62D-cysteine desulfhydrase OS=Salmonella paratyphi B... [more]
DCYD_SALNS3.335e-2538.62D-cysteine desulfhydrase OS=Salmonella newport (st... [more]
DCYD_SALHS3.335e-2538.62D-cysteine desulfhydrase OS=Salmonella heidelberg ... [more]
DCYD_SALG23.335e-2538.62D-cysteine desulfhydrase OS=Salmonella gallinarum ... [more]

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Properties
Property NameValue
Genbank descriptionIC0AAA100BE01RM1 CitNFL Citrus clementina cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY262577 ID=DY262577; Name=DY262577; organism=Citrus clementina; type=EST; length=1203bp
TTTTGGATTTTGGTAGTGTCATGCAGCAGCAGCAGCGGTTAAAGCAAATG
GAATCATGTTGTTGTGCAGCAGCTGCAGCGGTAACCTTCTGAGTTCAATT
GTTTCTCCAAAATTAAGACAACGCCTAATCAGAGTCTCTGAGTCTCAGCT
TCATCATCATCAGCAGCATAAAATCAAAATGGAAAGAAAAGAGAGCGGTA
ATGATAGTGATGCTTTCGGTTTCAAATTCCTCACCAAAACCCCATACGCT
TCTCCTTCTTGGGCTTCTCATCTCACCCCCATCCCTTCTCACGTCTTCTC
TCTCGGCCACTTTCCAACTCCAATTCACAAATGGAACCTCCCTAATTTGC
CCCATAACACTGAGGTCTGGCTCAAGCGTGATGACCTTTCCGGCATGCAA
TTGAGTGGAAACAAGGTAAGGAAATTAGAGTTTTTGATGGCAGATGCAGT
GGCTCAAGGTGCTGATTGCATCATCACCATTGGAGGCATCCAAAGCAATC
ATTGTCGTGCTGCTGCCGTTGCTGCCAAGTATCTCAATCTTGATTGTTAT
CTTATTCTTCGCACCTCTAAGGTTCTGGTGGACCAAGATCCTGGTTTGAC
TGGCAATCTACTAGTTGAACGTTTAGTTGGAGCTCATATTGAACTTATCT
CTAAAGAAGAGTATTCAAAAATTGGGAGTGTGACTCTTACTAACATTTTA
AAAGAAAAGTTGCTAAAGGAAGGGAGAAGACCATATGTTATTCCTGTTGG
TGGGTCAAACTCAATAGGGACATGGGGTTATATTGAAGCAATTAAGGAGA
TTGAGCAGNCACTTCAGACTGGGACTGGTGGAGTGAAATTGATGATATTG
TTGTAGCTTGTGGCAGTGGAGGAACAATTGCTGGTTTGTCATTGGGGTCA
TGGCTTGGAACATTGAAAGCAAAAGTTCATGCTTTTTCTGTTTGTGATGA
CCCTGATTACTTCTACGACTATACCCAAGGGCTACTTGATGGGCTCAATG
CAGGAATTGACTCCCATGATTTGGTAAACATTCAAAATCCAAAGGTTCTG
GCTATAGCATCACATTTCGGAAGAACTAATTTTGTTAAAAAATTGGTGCC
GCAACGGGGGTAATTTTTGATCAGTTTAAAAGGGAAAAAATTGCCCTAGG
GGGAGTGGAAAAAAAAGGGCTTAAAAATCCCAAAAAAGGGGGGAAAGGGG
AGG
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