A localized linkage map of the citrus tristeza virus resistance gene region

Publication Overview
TitleA localized linkage map of the citrus tristeza virus resistance gene region
AuthorsGmitter FJ, Xiao S, Huang S, Hu X, Garnsey S, Deng Z
TypeJournal Article
Journal NameTheoretical and applied genetics
Volume92
Issue6
Year1996
Page(s)688-695
CitationGmitter FJ, Xiao S, Huang S, Hu X, Garnsey S, Deng Z. A localized linkage map of the citrus tristeza virus resistance gene region. Theoretical and applied genetics. 1996 May; 92(6):688-695.

Abstract

A localized genetic linkage map was developed of the region surrounding the citrus tristeza virus (CTV) resistance gene (designated Ctv) from Poncirus trifoliata L., a sexually compatible Citrus relative. Bulked segregant analysis (BSA) was used to identify potential resistance-associated RAPD fragment markers in four intergeneric backcross families that were segregating for CTV resistance. Eight RAPD fragments were found that were consistently linked to Ctv in the four families. Map distances and locus order were determined with MAPMAKER 3.0, using the results obtained from 59 individuals in the largest family. Also, a consensus map was constructed with JOINMAP 1.3, using pooled results from the four backcross families. Marker orders were identical, except for 1 marker, on these independently developed maps. Family-specific resistance-associated markers were also identified, as were numerous susceptibility-associated markers. The identification of markers tightly linked to Ctv will enable citrus breeders to identify plants likely to be CTV-resistant by indirect, marker-assisted selection, rather than by labor-intensive direct challenge with the pathogen. These markers also provide a basis for future efforts to isolate Ctv for subsequent genetic manipulation.
Stocks
This publication contains information about 20 stocks:
Stock NameUniquenameType
AmbersweetAmbersweetcultivar
CarrizoCarrizobreeding_research_material
FosterFostercultivar
LB1-21LB1-21cultivar
LB7-5LB7-5cultivar
PummeloPummelocultivar
ThongDeeThongDeebreeding_research_material
US119US119cultivar
USDA17-40USDA17-40cultivar
USDA17-47USDA17-47cultivar
English largeEnglish largecultivar
KryderKrydercultivar
PomeroyPomeroycultivar
Ambersweet_x_US119Ambersweet_x_US119population
Foster_x_KryderFoster_x_Kryderpopulation
LB1-21_x_CarrizoLB1-21_x_Carrizopopulation
LB7-5_x_USDA17-47LB7-5_x_USDA17-47population
pummelo_x_EnglishLargepummelo_x_EnglishLargepopulation
Citrus-ThongDee/USDA17-40-BC1Citrus-ThongDee/USDA17-40-BC1population
Citrus-Consensus-R/U/LB1/LB2Citrus-Consensus-R/U/LB1/LB2population
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date1996 May
Language Abbreng
Journal AbbreviationTheor. appl. genet.
KeywordsPoncirus trifoliata, genetic markers, linkage groups, genes, disease resistance, Citrus tristeza virus, chromosome mapping, hybrids, Citrus, intergeneric hybridization, linkage (genetics), random amplified polymorphic DNA technique, segregation, ctv gene
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:3177468
PMID: PubMedPMID:24166392