Developing fruit peel at 38 DAFB

Overview
Library NameDeveloping fruit peel at 38 DAFB
Unique NameDeveloping fruit peel at 38 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 17, 2003, between 8 to 9 am and stored at 4C. The entire peel (including both flavedo and albedo) was used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
SNP Chip Base
Array NameDeveloping fruit peel at 38 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
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Feature NameUnique NameType
CK935089CK935089EST
CK935088CK935088EST
CK935087CK935087EST
CK935086CK935086EST
CK935085CK935085EST
CK935084CK935084EST
CK935083CK935083EST
CK935082CK935082EST
CK935081CK935081EST
CK935080CK935080EST
CK935079CK935079EST
CK935078CK935078EST
CK935077CK935077EST
CK935076CK935076EST
CK935075CK935075EST
CK935074CK935074EST
CK935073CK935073EST
CK935072CK935072EST
CK935071CK935071EST
CK935070CK935070EST
CK935069CK935069EST
CK935068CK935068EST
CK935067CK935067EST
CK935066CK935066EST
CK935065CK935065EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageYoung fruit sample-collected June 17, 2003
Genbank library noteOrgan: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 17, 2003, between 8 to 9 am and stored at 4C. The entire peel (including both flavedo and albedo) was used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Fruittissue type