FC869246

Overview
NameFC869246
Unique NameFC869246
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length685
Libraries
Library NameType
AbioticL1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of FC869246 vs. ExPASy Swiss-Prot
Match: DHGY_CUCSA (Glycerate dehydrogenase OS=Cucumis sativus GN=HPR-A PE=2 SV=1)

HSP 1 Score: 374.785 bits (961), Expect = 2.370e-103
Identity = 185/214 (86.45%), Postives = 193/214 (90.19%), Query Frame = 2
Query:   44 MAKPVSIEVCHPNGKYRVVSTKPMPGTRWINLLIEQDCRVEICTQKKTILSVEDIIALIGDKCDGVIGQLTEDWGETLFAALSRAGGKAFSNMAVGYNNVDVNAANKYGIAVGNTPGVXXXXXXXXXXXXXXXXXRRIVEADEFMRAGLYDGWLPNLFVGNLLKGQTVGVIGAGRIGSAYARMMVEGFKMNLIYYDLYQATRLEKFVTAYGQFL 685
            MAKPV IEV +PNGKYRVVSTKPMPGTRWINLLIEQDCRVEICT+KKTILSVEDI+ALIGDKCDGVIGQLTEDWGE LF+ALSRAGGKAFSNMAVGYNNVDVNAANKYG+AVGNTPGV                 RRIVEADEFMRAG YDGWLPNLFVGNLLKGQTVGVIGAGRIGSAYARMMVEGFKMNLIY+DLYQ+TRLEKFVTAYG+FL
Sbjct:    1 MAKPVQIEVWNPNGKYRVVSTKPMPGTRWINLLIEQDCRVEICTEKKTILSVEDILALIGDKCDGVIGQLTEDWGEVLFSALSRAGGKAFSNMAVGYNNVDVNAANKYGVAVGNTPGVLTETTAELAASLSLAAARRIVEADEFMRAGRYDGWLPNLFVGNLLKGQTVGVIGAGRIGSAYARMMVEGFKMNLIYFDLYQSTRLEKFVTAYGEFL 214          
BLAST of FC869246 vs. ExPASy Swiss-Prot
Match: GYAR_KORCO (Glyoxylate reductase OS=Korarchaeum cryptofilum (strain OPF8) GN=gyaR PE=3 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 2.350e-18
Identity = 53/144 (36.81%), Postives = 78/144 (54.17%), Query Frame = 2
Query:  230 CDGVIGQLTEDWGETLFAALSRAGGKAFSNMAVGYNNVDVNAANKYGIAVGNTPGVXXXXXXXXXXXXXXXXXRRIVEADEFMRAGLYD-GWLPNLFVGNLLKGQTVGVIGAGRIGSAYARMMVEGFKMNLIYYDLYQATRLEK 658
            CD ++  LT+     +F A  +   +  +  AVGY+N+DV  A K GI V NTPGV                 RR+VEAD ++R G +   W P + +G  + G+T+G++G GRIG+A AR   +GF M ++YYD  +    EK
Sbjct:   46 CDALVSLLTDPIDAEVFEAAPKL--RIVAQYAVGYDNIDVKEATKRGIYVTNTPGVLTETTADFAFALLMAAARRVVEADRYVREGKWKVAWHPMMMLGYDVYGRTLGIVGMGRIGAAVAR-RAKGFGMRILYYDSIRREDFEK 186          
BLAST of FC869246 vs. ExPASy Swiss-Prot
Match: GYAR_THELI (Glyoxylate reductase OS=Thermococcus litoralis GN=gyaR PE=1 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 5.235e-18
Identity = 52/126 (41.27%), Postives = 71/126 (56.35%), Query Frame = 2
Query:  305 KAFSNMAVGYNNVDVNAANKYGIAVGNTPGVXXXXXXXXXXXXXXXXXRRIVEADEFMRAGLYD----GWLPNLFVGNLLKGQTVGVIGAGRIGSAYARMMVEGFKMNLIYYDLYQATRLEKFVTA 670
            K  +  AVGY+N+D+  A K GI V NTPGV                 RRIVEAD F+R+G +     GW P +F+G  LKG+T+G++G GRIG A A+   +GF M +IYY   +    E+ + A
Sbjct:   69 KIIAQYAVGYDNIDIEEATKRGIYVTNTPGVLTDATADLAFALLLAVARRIVEADAFVRSGEWKKSEVGWHPLMFLGYGLKGKTLGIVGFGRIGQALAK-RAKGFGMKIIYYSRTRKPEAEEEIGA 193          
BLAST of FC869246 vs. ExPASy Swiss-Prot
Match: GYAR_PYRFU (Glyoxylate reductase OS=Pyrococcus furiosus GN=gyaR PE=3 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 6.836e-18
Identity = 63/195 (32.31%), Postives = 103/195 (52.82%), Query Frame = 2
Query:   86 KYRVVSTKPMPGTRWINLLIEQDCRVEICTQKKTILSVEDIIALIGDKCDGVIGQLTEDWGETLFAALSRAGGKAFSNMAVGYNNVDVNAANKYGIAVGNTPGVXXXXXXXXXXXXXXXXXRRIVEADEFMRAGLYD----GWLPNLFVGNLLKGQTVGVIGAGRIGSAYARMMVEGFKMNLIYYDLYQATRLEK 658
            K +V  T+ +P    IN+L E++  VE+  +++ I   E ++  + D  D ++  L+E   + +F    R   +  +N AVGY+N+DV  A + GI V NTP V                 R +V+ D+F+R+G +      W P  F+G  L G+T+G++G GRIG A AR   +GF M ++YY   + ++ EK
Sbjct:    2 KPKVFITRAIP-ENGINML-EEEFEVEVWEEEREIPR-EKLLEKVKD-VDALVTMLSERIDQEVFENAPRL--RIVANYAVGYDNIDVEEATRRGIYVTNTPDVLTNATADHAFALLLATARHVVKGDKFVRSGEWKRKGIAWHPKWFLGYELYGKTIGIVGFGRIGQAIAR-RAKGFNMRILYYSRTRKSQAEK 189          
BLAST of FC869246 vs. ExPASy Swiss-Prot
Match: GYAR_PYRAB (Glyoxylate reductase OS=Pyrococcus abyssi GN=gyaR PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 9.872e-17
Identity = 58/181 (32.04%), Postives = 92/181 (50.83%), Query Frame = 2
Query:  140 LIEQDCRVEICTQKKTILSVEDIIALIGDKCDGVIGQLTEDWGETLFAALSRAGGKAFSNMAVGYNNVDVNAANKYGIAVGNTPGVXXXXXXXXXXXXXXXXXRRIVEADEFMRAGLYD----GWLPNLFVGNLLKGQTVGVIGAGRIGSAYARMMVEGFKMNLIYYDLYQATRLEKFVTA 670
            ++E++  VE+   ++ I   E ++  + D  D ++  L+E     +F    R   +  +N AVGY+N+DV  A K GI V NTPGV                 R +V+ D+F R+G +      W P  F+G  + G+T+G+IG GRIG A A+    GF M ++YY   +   +EK + A
Sbjct:   19 MLEKEFEVEVWEDEREIPR-EILLEKVKD-VDALVTMLSERIDREVFERAPRL--RIVANYAVGYDNIDVEEATKRGIYVTNTPGVLTDATADLAFALLLATARHLVKGDKFTRSGEWKKRGVAWHPKWFLGYDVYGKTIGIIGFGRIGQAIAK-RARGFDMRILYYSRTRKPEVEKELNA 194          
BLAST of FC869246 vs. ExPASy Swiss-Prot
Match: GYAR_THEON (Glyoxylate reductase OS=Thermococcus onnurineus (strain NA1) GN=gyaR PE=3 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 1.289e-16
Identity = 63/195 (32.31%), Postives = 101/195 (51.79%), Query Frame = 2
Query:   86 KYRVVSTKPMPGTRWINLLIEQDCRVEICTQKKTILSVEDIIALIGDKCDGVIGQLTEDWGETLFAALSRAGGKAFSNMAVGYNNVDVNAANKYGIAVGNTPGVXXXXXXXXXXXXXXXXXRRIVEADEFMRAGLYD----GWLPNLFVGNLLKGQTVGVIGAGRIGSAYARMMVEGFKMNLIYYDLYQATRLEK 658
            K +V+ T+ +P    I +L E    VE+   +  I S E ++  + D  D ++  L+E     +F A  R   K  +N AVGY+N+D+  A K G+ + NTP V                 RR++EAD+F+R+G +      W P +F+G  + G+T+G++G GRIG A AR   +GF M ++Y    +   +EK
Sbjct:    2 KPKVLITRKIP-ENGIKMLREH-FEVEVWEDEHEI-SREVLLEKVRD-VDALVTMLSERIDAEVFDAAPRL--KIVANYAVGYDNIDIEEATKMGVYITNTPDVLTNATADMAWVLLLATARRLIEADKFVRSGEWKKRGVAWHPLMFLGYDVYGRTIGIVGFGRIGQAIAR-RAKGFGMRILYNSRTRKPEVEK 189          
BLAST of FC869246 vs. ExPASy Swiss-Prot
Match: GYAR_PYRKO (Glyoxylate reductase OS=Pyrococcus kodakaraensis GN=gyaR PE=3 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 1.684e-16
Identity = 57/177 (32.20%), Postives = 90/177 (50.85%), Query Frame = 2
Query:  140 LIEQDCRVEICTQKKTILSVEDIIALIGDKCDGVIGQLTEDWGETLFAALSRAGGKAFSNMAVGYNNVDVNAANKYGIAVGNTPGVXXXXXXXXXXXXXXXXXRRIVEADEFMRAGLYD----GWLPNLFVGNLLKGQTVGVIGAGRIGSAYARMMVEGFKMNLIYYDLYQATRLEK 658
            ++++   VE+  +++ I   E ++  + D  D ++  L+E     +F A  R   +  +N AVGY+N+DV  A + GI V NTP V                 RR++EAD F R+G +      W P  F+G  + G+T+G++G GRIG A AR    GF M ++YY   +    EK
Sbjct:   18 MLKEHFEVEVWPEEREIPR-EVLLKKVRD-VDALVTMLSERIDSEVFDAAPRL--RIVANYAVGYDNIDVEEATRRGIYVTNTPDVLTDATADFAWTLLLATARRLIEADHFTRSGEWKRRGIAWHPRWFLGYDVYGKTIGIVGFGRIGQAVAR-RARGFGMRILYYSRSRKPEAEK 189          
BLAST of FC869246 vs. ExPASy Swiss-Prot
Match: GYAR_THEPD (Glyoxylate reductase OS=Thermofilum pendens (strain Hrk 5) GN=gyaR PE=3 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 2.872e-16
Identity = 61/194 (31.44%), Postives = 96/194 (49.48%), Query Frame = 2
Query:   86 KYRVVSTKPMPGTRWINLLIEQDCRVEICTQKKTILSVEDIIALIGDKCDGVIGQLTEDWGETLFAALSRAGGKAFSNMAVGYNNVDVNAANKYGIAVGNTPGVXXXXXXXXXXXXXXXXXRRIVEADEFMRAGLYDG-WLPNLFVGNLLKGQTVGVIGAGRIGSAYARMMVEGFKMNLIYYDLYQATRLEKFV 664
            K  V    P PG      ++++ C V +   K    S E+++  I DK D ++  LT+     +  A      K  S  +VG++++D+  A K GI V +TPGV                 RRIVEAD+ +R G +D  W P    G  LKG+T+G++G GRIG A A+ +   F + ++YYD+ +   +E  +
Sbjct:    5 KVYVTRIIPEPGLS----MLKECCDVVVHESKDWPPSREELLRNIRDK-DALLCLLTDKIDAEVMDAAPNL--KVISTYSVGFDHIDIPEATKRGIYVTHTPGVLTDAVAEFTVGLILAVTRRIVEADKIIRTGQWDKPWNPYFLTGPELKGKTIGLVGLGRIGVATAKRL-SSFDVKILYYDIERRWDVETVI 190          
BLAST of FC869246 vs. ExPASy Swiss-Prot
Match: GYAR_PYRHO (Glyoxylate reductase OS=Pyrococcus horikoshii GN=gyaR PE=1 SV=2)

HSP 1 Score: 84.7297 bits (208), Expect = 4.899e-16
Identity = 54/181 (29.83%), Postives = 91/181 (50.28%), Query Frame = 2
Query:  140 LIEQDCRVEICTQKKTILSVEDIIALIGDKCDGVIGQLTEDWGETLFAALSRAGGKAFSNMAVGYNNVDVNAANKYGIAVGNTPGVXXXXXXXXXXXXXXXXXRRIVEADEFMRAGLYD----GWLPNLFVGNLLKGQTVGVIGAGRIGSAYARMMVEGFKMNLIYYDLYQATRLEKFVTA 670
            ++E +  VE+   +K I    +I+     + D ++  L+E   + +F    +   +  +N AVGY+N+D+  A K GI V NTP V                 R +V+ D F+R+G +      W P  F+G  + G+T+G+IG GRIG A A+   +GF M ++YY   +   +E+ + A
Sbjct:   18 MLEDEFEVEVWGDEKEI--PREILLKKVKEVDALVTMLSERIDKEVFENAPKL--RIVANYAVGYDNIDIEEATKRGIYVTNTPDVLTDATADLAFALLLATARHVVKGDRFVRSGEWKKRGVAWHPKWFLGYDVYGKTIGIIGLGRIGQAIAK-RAKGFNMRILYYSRTRKEEVERELNA 193          
BLAST of FC869246 vs. ExPASy Swiss-Prot
Match: GYAR_THEGJ (Glyoxylate reductase OS=Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3) GN=gyaR PE=3 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 6.399e-16
Identity = 58/181 (32.04%), Postives = 93/181 (51.38%), Query Frame = 2
Query:  140 LIEQDCRVEICTQKKTILSVEDIIALIGDKCDGVIGQLTEDWGETLFAALSRAGGKAFSNMAVGYNNVDVNAANKYGIAVGNTPGVXXXXXXXXXXXXXXXXXRRIVEADEFMRAGLYD----GWLPNLFVGNLLKGQTVGVIGAGRIGSAYARMMVEGFKMNLIYYDLYQATRLEKFVTA 670
            ++ +   VE+  +++ I   E ++  + D  D ++  L+E     +F    R   +  +N AVGY+NVDV  A + GI V NTP V                 RR++EAD F R+G +      W P +F+G+ + G+T+G+IG GRIG A AR   +GF M ++YY   +    E+ + A
Sbjct:   18 MLREHFEVEVWPEEREIPR-EVLLEKVRD-VDALVTMLSERIDGEVFDNAPRL--RIVANYAVGYDNVDVEEATRRGIYVTNTPDVLTNATADFAWTLLLATARRLIEADSFTRSGEWKRKGIAWHPLMFLGHDVYGKTIGIIGFGRIGQAVAR-RAKGFGMRILYYSRTRKPEAEEELKA 193          
The following BLAST results are available for this feature:
BLAST of FC869246 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 26
Match NameE-valueIdentityDescription
DHGY_CUCSA2.370e-10386.45Glycerate dehydrogenase OS=Cucumis sativus GN=HPR-... [more]
GYAR_KORCO2.350e-1836.81Glyoxylate reductase OS=Korarchaeum cryptofilum (s... [more]
GYAR_THELI5.235e-1841.27Glyoxylate reductase OS=Thermococcus litoralis GN=... [more]
GYAR_PYRFU6.836e-1832.31Glyoxylate reductase OS=Pyrococcus furiosus GN=gya... [more]
GYAR_PYRAB9.872e-1732.04Glyoxylate reductase OS=Pyrococcus abyssi GN=gyaR ... [more]
GYAR_THEON1.289e-1632.31Glyoxylate reductase OS=Thermococcus onnurineus (s... [more]
GYAR_PYRKO1.684e-1632.20Glyoxylate reductase OS=Pyrococcus kodakaraensis G... [more]
GYAR_THEPD2.872e-1631.44Glyoxylate reductase OS=Thermofilum pendens (strai... [more]
GYAR_PYRHO4.899e-1629.83Glyoxylate reductase OS=Pyrococcus horikoshii GN=g... [more]
GYAR_THEGJ6.399e-1632.04Glyoxylate reductase OS=Thermococcus gammatolerans... [more]

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Properties
Property NameValue
Genbank descriptionC31010B05EF AbioticL1 Citrus clementina cDNA clone C31010B05, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC869246 ID=FC869246; Name=FC869246; organism=Citrus clementina; type=EST; length=685bp
CTGTTTTTGGCATCTGGAACTTGAAACTGCCAACTTAGCTTCAATGGCGA
AGCCAGTTTCAATTGAAGTGTGCCACCCAAATGGAAAATACAGAGTTGTT
AGTACCAAGCCTATGCCTGGAACCCGCTGGATCAATCTCTTAATCGAACA
AGATTGCCGTGTTGAAATATGCACCCAGAAGAAAACTATACTCTCTGTTG
AAGATATCATTGCTTTGATTGGTGACAAGTGCGATGGAGTCATTGGACAG
TTGACAGAGGACTGGGGAGAGACTTTGTTTGCTGCACTGAGTAGAGCAGG
AGGAAAGGCTTTTAGTAACATGGCTGTTGGTTACAACAATGTTGACGTTA
ATGCTGCTAACAAATATGGTATTGCTGTTGGAAACACTCCTGGAGTACTT
ACTGAAACAACAGCAGAGTTAGCAGCTTCGCTCTCTCTAGCGGCTGCAAG
AAGAATTGTTGAAGCAGATGAGTTTATGAGAGCTGGCTTATACGATGGAT
GGCTTCCAAATCTATTCGTCGGGAACTTGCTCAAGGGACAGACTGTCGGT
GTGATTGGAGCTGGTCGTATCGGATCTGCCTATGCTAGAATGATGGTTGA
AGGGTTCAAAATGAACCTCATTTACTATGATCTTTACCAAGCTACACGCT
TGGAAAAGTTTGTCACAGCTTATGGTCAGTTTCTA
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