FC876836

Overview
NameFC876836
Unique NameFC876836
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length755
Libraries
Library NameType
EHRcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of FC876836 vs. ExPASy Swiss-Prot
Match: C81D1_ARATH (Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 7.056e-38
Identity = 75/153 (49.02%), Postives = 101/153 (66.01%), Query Frame = -1
Query:  300 MLNDVDLVNLPYLCCVIKETXXXXXXXXXXXPHFSSENCIVGGYHIPRGTTIMVNAWAMHRDSKVWEEPNKFKPERFEGIHSEREGFKHIPFGMGRRACPGAAMAIRTISFALGSLIQCFEWEKIG-AEVDMTASYGLSLFKTVPLVAMCSPR 755
            ++ + DL  LPYL  ++ ET           PH +SE+C +G Y +PRGTT++VNAWA+HRD   W++P+ FKPERFE    E E  K + FG+GRRACPG+ +A R +  ALGSLIQCFEWE++G  EVDM    G ++ K +PL A+C  R
Sbjct:  345 LIEEADLSELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFE---KEEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTVPKAIPLKAICKAR 494          
BLAST of FC876836 vs. ExPASy Swiss-Prot
Match: C81F1_ARATH (Cytochrome P450 81F1 OS=Arabidopsis thaliana GN=CYP81F1 PE=2 SV=2)

HSP 1 Score: 150.599 bits (379), Expect = 8.625e-36
Identity = 75/161 (46.58%), Postives = 104/161 (64.60%), Query Frame = -1
Query:  279 MLNDVDLVNLPYLCCVIKETXXXXXXXXXXXPHFSSENCIVGGYHIPRGTTIMVNAWAMHRDSKVWEEPNKFKPERF-EGIHSEREGFKHIPFGMGRRACPGAAMAIRTISFALGSLIQCFEWEKI-GAEVDMTASYGLSLFKTVPLVAMCSPRQNMIGML 755
            ++++ D+  LPYL  V+ ET           P   +++  +GGY +PR T +MVNAWA+HRD ++WEEP KF P+R+ +G  S+   +K +PFG GRR CPGA +  R ++ ALGSLIQCFEWE + G E+DM+ S GL + K  PL AMC PR  M  +L
Sbjct:  339 LIDESDIAVLPYLQNVVSETFRLFPVAPFLIPRSPTDDMKIGGYDVPRDTIVMVNAWAIHRDPEIWEEPEKFNPDRYNDGCGSDYYVYKLMPFGNGRRTCPGAGLGQRIVTLALGSLIQCFEWENVKGEEMDMSESTGLGMRKMDPLRAMCRPRPIMSKLL 499          
BLAST of FC876836 vs. ExPASy Swiss-Prot
Match: C81E1_GLYEC (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 138.658 bits (348), Expect = 3.392e-32
Identity = 72/153 (47.06%), Postives = 95/153 (62.09%), Query Frame = -1
Query:  300 MLNDVDLVNLPYLCCVIKETXXXXXXXXXXXPHFSSENCIVGGYHIPRGTTIMVNAWAMHRDSKVWEEPNKFKPERFEGIHSEREGFKHIPFGMGRRACPGAAMAIRTISFALGSLIQCFEWEKI-GAEVDMTASYGLSLFKTVPLVAMCSPR 755
            ++++ DL  L YL  VI ET           PH +S+ C +GGY +P+ T +++NAWA+HRD ++W E   FKPERFE    + E  K I FGMGRRACPG  +AIR IS  L  LIQCF+W+ I G ++D+    G +L K VPL AMC  R
Sbjct:  341 LVDESDLPKLTYLKNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFE---KKGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGFTLTKLVPLKAMCKSR 490          
BLAST of FC876836 vs. ExPASy Swiss-Prot
Match: F3PH_ARATH (Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana GN=CYP75B1 PE=1 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 6.396e-31
Identity = 68/161 (42.24%), Postives = 94/161 (58.39%), Query Frame = -1
Query:  300 LNDVDLVNLPYLCCVIKETXXXXXXXXXXXPHFSSENCIVGGYHIPRGTTIMVNAWAMHRDSKVWEEPNKFKPERF------EGIHSEREGFKHIPFGMGRRACPGAAMAIRTISFALGSLIQCFEWEKIGA----EVDMTASYGLSLFKTVPLVAMCSPR 752
            +N+ D+  LPYL  VIKE            PH +SE+C + GYHIP+G+T++ N WA+ RD   W +P  FKPERF       G+  +   F+ IPFG GRR C G ++ +RTI F   +L+Q F+WE  G     +++M  SYGL+L + VPLV    PR
Sbjct:  342 VNESDIAQLPYLQAVIKENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPERFLPGGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVTPEKLNMEESYGLTLQRAVPLVVHPKPR 502          
BLAST of FC876836 vs. ExPASy Swiss-Prot
Match: C75A1_PETHY (Flavonoid 3',5'-hydroxylase 1 OS=Petunia hybrida GN=CYP75A1 PE=2 SV=1)

HSP 1 Score: 124.405 bits (311), Expect = 6.619e-28
Identity = 69/159 (43.40%), Postives = 92/159 (57.86%), Query Frame = -1
Query:  300 LNDVDLVNLPYLCCVIKETXXXXXXXXXXXPHFSSENCIVGGYHIPRGTTIMVNAWAMHRDSKVWEEPNKFKPERF-----EGIHSEREGFKHIPFGMGRRACPGAAMAIRTISFALGSLIQCFEWEKIGAEV---DMTASYGLSLFKTVPLVAMCSPR 752
            L + D+ NLPYL  + KET           P  S+E CIV GY+IP+ T + VN WA+ RD +VWE P +F PERF       I      F+ IPFG GRR C G  M I  + + LG+L+  F+W K+ +EV   +M  ++GL+L K VPL AM +PR
Sbjct:  341 LLESDIPNLPYLRAICKETFRKHPSTPLNLPRISNEPCIVDGYYIPKNTRLSVNIWAIGRDPQVWENPLEFNPERFLSGRNSKIDPRGNDFELIPFGAGRRICAGTRMGIVMVEYILGTLVHSFDW-KLPSEVIELNMEEAFGLALQKAVPLEAMVTPR 498          
BLAST of FC876836 vs. ExPASy Swiss-Prot
Match: C75A3_PETHY (Flavonoid 3',5'-hydroxylase 2 OS=Petunia hybrida GN=CYP75A3 PE=2 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 1.129e-27
Identity = 69/159 (43.40%), Postives = 92/159 (57.86%), Query Frame = -1
Query:  300 LNDVDLVNLPYLCCVIKETXXXXXXXXXXXPHFSSENCIVGGYHIPRGTTIMVNAWAMHRDSKVWEEPNKFKPERF-----EGIHSEREGFKHIPFGMGRRACPGAAMAIRTISFALGSLIQCFEWEKIGAEV---DMTASYGLSLFKTVPLVAMCSPR 752
            L + D+ NLPYL  + KET           P  S+E CIV GY+IP+ T + VN WA+ RD +VWE P +F PERF       I      F+ IPFG GRR C G  M I  + + LG+L+  F+W K+ +EV   +M  ++GL+L K VPL AM +PR
Sbjct:  341 LLESDIPNLPYLRAICKETFRKHPSTPLNLPRISNEPCIVDGYYIPKNTRLSVNIWAIGRDPEVWENPLEFYPERFLSGRNSKIDPRGNDFELIPFGAGRRICAGTRMGIVMVEYILGTLVHSFDW-KLPSEVIELNMEEAFGLALQKAVPLEAMVTPR 498          
BLAST of FC876836 vs. ExPASy Swiss-Prot
Match: C98A1_SORBI (Cytochrome P450 98A1 OS=Sorghum bicolor GN=CYP98A1 PE=2 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 1.926e-27
Identity = 66/164 (40.24%), Postives = 89/164 (54.27%), Query Frame = -1
Query:  273 DVDLVNLPYLCCVIKETXXXXXXXXXXXPHFSSENCIVGGYHIPRGTTIMVNAWAMHRDSKVWEEPNKFKPERF--EGIHSEREGFKHIPFGMGRRACPGAAMAIRTISFALGSLIQCFEWE----KIGAEVDMTASYGLSLFKTVPLVAMCSPRQNMIGMLNR 746
            + D  NLPYL  V+KE+           PH +S N  +GGY IP+G  +MVN WA+ RD KVW  P +++PERF  E I  +   F+ +PFG GRR CPGA + I  ++  +G L+  FEW         +V+M  S GL  F   PL A+  PR     + NR
Sbjct:  344 ETDFQNLPYLQAVVKESLRLHPPTPLMLPHKASTNVKIGGYDIPKGANVMVNVWAVARDPKVWSNPLEYRPERFLEENIDIKGSDFRVLPFGAGRRVCPGAQLGINLVASMIGHLLHHFEWSLPEGTRPEDVNMMESPGLVTFMGTPLQAVAKPRLEKEELYNR 507          
BLAST of FC876836 vs. ExPASy Swiss-Prot
Match: C75A6_CAMME (Flavonoid 3',5'-hydroxylase OS=Campanula medium GN=CYP75A6 PE=2 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 3.285e-27
Identity = 66/158 (41.77%), Postives = 88/158 (55.70%), Query Frame = -1
Query:  300 LNDVDLVNLPYLCCVIKETXXXXXXXXXXXPHFSSENCIVGGYHIPRGTTIMVNAWAMHRDSKVWEEPNKFKPERF-----EGIHSEREGFKHIPFGMGRRACPGAAMAIRTISFALGSLIQCFEWEKIG--AEVDMTASYGLSLFKTVPLVAMCSPR 752
            L   D+ NLPY   + KET           P  S+E C V G+HIP+ T ++VN WA+ RD KVWE P  F PERF       I      F+ IPFG GRR C GA M   ++ + LG+L+  F+W+      EV+M  S+G++L K VPL A+ +PR
Sbjct:  358 LEQSDIPNLPYFQAICKETFRKHPSTPLNLPRISTEACEVDGFHIPKNTRLIVNIWAIGRDPKVWENPLDFTPERFLSEKHAKIDPRGNHFELIPFGAGRRICAGARMGAASVEYILGTLVHSFDWKLPDGVVEVNMEESFGIALQKKVPLSAIVTPR 515          
BLAST of FC876836 vs. ExPASy Swiss-Prot
Match: C75A7_EUSGR (Flavonoid 3',5'-hydroxylase OS=Eustoma grandiflorum GN=CYP75A7 PE=2 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 9.558e-27
Identity = 63/156 (40.38%), Postives = 87/156 (55.77%), Query Frame = -1
Query:  300 DVDLVNLPYLCCVIKETXXXXXXXXXXXPHFSSENCIVGGYHIPRGTTIMVNAWAMHRDSKVWEEPNKFKPERF-----EGIHSEREGFKHIPFGMGRRACPGAAMAIRTISFALGSLIQCFEWEKIGA--EVDMTASYGLSLFKTVPLVAMCSPR 746
            + D+  LPYL  + KE            P  +S+ C V G++IP+GT + VN WA+ RD  VWE PN+F P+RF       I      F+ IPFG GRR C G  + I  + + LG+L+  F WE   +  E++M  S+GL+L K VPL AM +PR
Sbjct:  347 EADISKLPYLQAICKEAFRKHPSTPLNLPRIASQACEVNGHYIPKGTRLSVNIWAIGRDPSVWENPNEFNPDRFLERKNAKIDPRGNDFELIPFGAGRRICAGTRLGILLVEYILGTLVHSFVWELPSSVIELNMDESFGLALQKAVPLAAMVTPR 502          
BLAST of FC876836 vs. ExPASy Swiss-Prot
Match: C75A5_EUSGR (Flavonoid 3',5'-hydroxylase OS=Eustoma grandiflorum GN=CYP75A5 PE=2 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 2.781e-26
Identity = 61/156 (39.10%), Postives = 87/156 (55.77%), Query Frame = -1
Query:  300 DVDLVNLPYLCCVIKETXXXXXXXXXXXPHFSSENCIVGGYHIPRGTTIMVNAWAMHRDSKVWEEPNKFKPERF-----EGIHSEREGFKHIPFGMGRRACPGAAMAIRTISFALGSLIQCFEWEKIGA--EVDMTASYGLSLFKTVPLVAMCSPR 746
            + D+  LPYL  + KE            P  +S+ C V G++IP+GT + VN WA+ RD  +WE PN+F P+RF       I      F+ IPFG GRR C G  + I  + + LG+L+  F+WE   +  E++M   +GL+L K VPL AM +PR
Sbjct:  347 EADISKLPYLQAICKEAFRKHPSTPLNLPRIASQACEVNGHYIPKGTRLSVNIWAIGRDPSLWENPNEFNPDRFLERKNAKIDPRGNDFELIPFGAGRRICAGTRLGILLVEYILGTLVHSFDWELPSSVIELNMDEPFGLALQKAVPLAAMVTPR 502          
The following BLAST results are available for this feature:
BLAST of FC876836 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 242
Match NameE-valueIdentityDescription
C81D1_ARATH7.056e-3849.02Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CY... [more]
C81F1_ARATH8.625e-3646.58Cytochrome P450 81F1 OS=Arabidopsis thaliana GN=CY... [more]
C81E1_GLYEC3.392e-3247.06Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata ... [more]
F3PH_ARATH6.396e-3142.24Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana... [more]
C75A1_PETHY6.619e-2843.40Flavonoid 3',5'-hydroxylase 1 OS=Petunia hybrida G... [more]
C75A3_PETHY1.129e-2743.40Flavonoid 3',5'-hydroxylase 2 OS=Petunia hybrida G... [more]
C98A1_SORBI1.926e-2740.24Cytochrome P450 98A1 OS=Sorghum bicolor GN=CYP98A1... [more]
C75A6_CAMME3.285e-2741.77Flavonoid 3',5'-hydroxylase OS=Campanula medium GN... [more]
C75A7_EUSGR9.558e-2740.38Flavonoid 3',5'-hydroxylase OS=Eustoma grandifloru... [more]
C75A5_EUSGR2.781e-2639.10Flavonoid 3',5'-hydroxylase OS=Eustoma grandifloru... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAN3BA08RM1 EHR Citrus clementina cDNA clone KN0AAN3BA08, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC876836 ID=FC876836; Name=FC876836; organism=Citrus clementina; type=EST; length=755bp
TTTTTTTTTTTTTTTTTTTTTTTTTTTTACATAATTTTGCAGTTGGCAAT
GACTGTTGAAATCAAACCGATATTAAAAAACTACAGCAAAACCACAAAAC
AGGATTATCAAATCTAAAATTTGGTCACGCAATGCCATGGCATGAAGTGA
TCTACAGTCTCTAAAGTACTGCTCTACAAATTCCAGTAAATTTTACAACC
TTTATTGAAAGTCACCTGCAAGGTCCCCTGCAGGCCCCGAAAATTTCCAG
CGGCAAGTTTACCCTAACTGGATCTGTTCAGCATTCCAATCATATTTTGC
CGCGGACTGCACATAGCCACCAAAGGCACGGTCTTAAATAGAGATAGTCC
ATAACTTGCAGTCATGTCCACCTCCGCCCCTATCTTTTCCCACTCGAAGC
ACTGTATGAGCGAACCCAAAGCAAAAGAAATTGTACGGATAGCCATGGCT
GCACCTGGGCAAGCTCTCCTCCCCATGCCAAATGGAATATGTTTGAATCC
CTCTCTTTCACTATGAATCCCTTCAAATCTTTCAGGTTTGAACTTGTTAG
GCTCCTCCCACACCTTGGAATCCCTATGCATTGCCCAGGCATTCACCATT
ATCGTCGTGCCTCGTGGTATGTGATATCCACCCACTATGCAGTTCTCAGA
AGAAAAATGTGGCAACAGAAGTGGTGCCGGAGGATATAATCGCAGCGTCT
CTTTTATGACACAGCAAAGGTATGGAAGGTTGACAAGATCCACGTCATTT
AACAT
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