FC877175

Overview
NameFC877175
Unique NameFC877175
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length708
Libraries
Library NameType
EHRcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of FC877175 vs. ExPASy Swiss-Prot
Match: LIS_CLABR (S-linalool synthase OS=Clarkia breweri GN=LIS PE=2 SV=1)

HSP 1 Score: 161.384 bits (407), Expect = 4.370e-39
Identity = 90/200 (45.00%), Postives = 124/200 (62.00%), Query Frame = 2
Query:   29 WYETFASWLTEATWSKSGRT-PSMEEYLETGMISIAAHTLVLTASCFLNPSLPNYK-FRPAQYDTVTKLLMLVPRLLNDIQSYQKEIADGKQNSVILYLRENPEADIEDSVAYVRXXXXXXXXXXXXXXXMDGFSDLPRPCKQLHLFCMKAFQMFFHSSNRYDSNTEMIDDVQKAFYIPLKLQTPKPLIKLMLNQLPLPL 622
            WYETF SWL EA W K   + PS++ YL   M SIAAHT+VLTASC L P  P ++ +   ++  +T LLM++ RLLNDIQSY KE  +GK N V +Y+ EN +A I+DSV +V+                D   +LP+  KQLH  C+K F MFF+SSN +D++T+++ D+ +AF  P ++   KP IK   +QLP  L
Sbjct:  612 WYETFHSWLMEAKWGKGLTSKPSVDVYLGNAMTSIAAHTMVLTASCLLGPGFPVHQLWSQRRHQDITSLLMVLTRLLNDIQSYLKEEDEGKINYVWMYMIENNQASIDDSVRHVQTIINVKKQEFIQRVLSDQHCNLPKSFKQLHFSCLKVFNMFFNSSNIFDTDTDLLLDIHEAFVSPPQVPKFKPHIK-PPHQLPATL 810          
BLAST of FC877175 vs. ExPASy Swiss-Prot
Match: KSB_ARATH (Ent-kaur-16-ene synthase, chloroplastic OS=Arabidopsis thaliana GN=GA2 PE=1 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 7.749e-20
Identity = 57/188 (30.32%), Postives = 94/188 (50.00%), Query Frame = 2
Query:   11 YELIMKWYETFASWLTEATWSKSGRTPSMEEYLETGMISIAAHTLVLTASCFLNPSLPNYKFRPAQYDTVTKLLMLVPRLLNDIQSYQKEIADGKQNSVILYLR-ENPEADIEDSVAYVRXXXXXXXXXXXXXXXMDGFSDLPRPCKQLHLFCMKAFQMFFHSSNRYDSNTEMIDDVQKAFYIPLKLQ 571
            + ++  W +   S L EA WS    TPS+E+Y+E   IS A   +VL A+  + P LP       QY+ + KL+  + RLLNDIQ +++E A+GK N+V L+++ E      E  +  ++                +  S +PR CK+  L   K   +F+   + + SN +++  V+   Y P+ LQ
Sbjct:  594 HHIVKIWLDLLKSMLREAEWSSDKSTPSLEDYMENAYISFALGPIVLPATYLIGPPLPEKTVDSHQYNQLYKLVSTMGRLLNDIQGFKRESAEGKLNAVSLHMKHERDNRSKEVIIESMKGLAERKREELHKLVLEEKGSVVPRECKEAFLKMSKVLNLFYRKDDGFTSN-DLMSLVKSVIYEPVSLQ 780          
BLAST of FC877175 vs. ExPASy Swiss-Prot
Match: KS1_ORYSJ (Ent-kaur-16-ene synthase, chloroplastic OS=Oryza sativa subsp. japonica GN=KS1 PE=1 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.237e-17
Identity = 54/185 (29.19%), Postives = 83/185 (44.86%), Query Frame = 2
Query:   29 WYETFASWLTEATWSKSGRTPSMEEYLETGMISIAAHTLVLTASCFLNPSLPNYKFRPAQYDTVTKLLMLVPRLLNDIQSYQKEIADGKQNSVILYLRENPEADIEDSVAYVRXXXXXXXXXXXXXXXMDGFSDLPRPCKQLHLFCMKAFQMFFHSSNRYDSNTEMIDDVQKAFYIPLKLQTPKP 583
            W     S +TEA W ++   P+MEEY+   ++S A   +VL    F+ P L     R  +Y+ + +L+    RLLND Q +++E  +GK NSV L +  +  +   D                     +     +PRPCKQL     K   MF+  ++ + S  EM+  V      PLKL+   P
Sbjct:  564 WLCLMRSMMTEAEWQRTKYVPTMEEYMANAVVSFALGPIVLPTLYFVGPKLQEDVVRDHEYNELFRLMSTCGRLLNDSQGFERESLEGKLNSVSLLVHHSGGSISIDEAKMKAQKSIDTSRRNLLRLVLGEQGAVPRPCKQLFWKMCKIVHMFYSRTDGFSSPKEMVSAVNAVVKEPLKLKVSDP 748          
BLAST of FC877175 vs. ExPASy Swiss-Prot
Match: KSL6_ORYSJ (Ent-isokaur-15-ene synthase OS=Oryza sativa subsp. japonica GN=KSL6 PE=1 SV=2)

HSP 1 Score: 84.7297 bits (208), Expect = 5.198e-16
Identity = 55/191 (28.80%), Postives = 87/191 (45.55%), Query Frame = 2
Query:   29 WYETFASWLTEATWSKSGRTPS-MEEYLETGMISIAAHTLVLTASCFLNPSLPNYKFRPAQYDTVTKLLMLVPRLLNDIQSYQKEIADGKQNSVILYL-------RENPEADIEDSVAYVRXXXXXXXXXXXXXXXMDGFSDLPRPCKQLHLFCMKAFQMFFHSSNRYDSNTEMIDDVQKAFYIPLKLQTP 577
            W+ T    LTEA W      P+ +EEY+   + S A   ++ +A+ F+ P L    FR  +Y  +  L   + RLLND+Q+Y+KEI  GK NSV+L+        R +PEA +E++   +R                D    +P PC++L     K     +   +RY +   M+         PL++  P
Sbjct:  625 WWFTVRGMLTEAEWRMDKYVPTTVEEYMSAAVDSFAVGPIITSAALFVGPELSEEVFRSEEYIHLMNLANTIGRLLNDMQTYEKEIKMGKVNSVMLHALSHSGGGRGSPEASMEEAKREMRRVLQGCRFELLRLVTRDA-GVVPPPCRKLFWLMSKVLHFVYMEKDRYFTAEGMMASANAVILDPLQVTLP 814          
BLAST of FC877175 vs. ExPASy Swiss-Prot
Match: KSL7_ORYSI (Ent-cassa-12,15-diene synthase OS=Oryza sativa subsp. indica GN=KSL7 PE=2 SV=2)

HSP 1 Score: 84.3445 bits (207), Expect = 6.788e-16
Identity = 49/188 (26.06%), Postives = 85/188 (45.21%), Query Frame = 2
Query:   17 LIMKWYETFASWLTEATWSKSGRTPSMEEYLETGMISIAAHTLVLTASCFLNPSLPNYKFRPAQYDTVTKLLMLVPRLLNDIQSYQKEIADGKQNSVILYL--RENPEADIEDSVAYVRXXXXXXXXXXXXXXXMDGFSDLPRPCKQLHLFCMKAFQMFFHSSNRYDSNTEMIDDVQKAFYIPLKLQT 574
            L+  W         EA W +S   P+ EEY+E+GM+S+     V++A   +   LP       +YD + +L+    RLLNDI+  ++E +DGK  + +  L         ++++   V                 +    +PRPCKQL     K   +F++ ++ + S  EM+  V      PL+L++
Sbjct:  642 LVDVWLHVVRCMKVEAEWQRSQHLPTFEEYMESGMVSLGQGATVMSALFLIGEKLPEGVVELEEYDEMFRLMGTCGRLLNDIRGIEREESDGKMTNGVSLLVHASGGSMSVDEAKTEVMKRIDASRRKLLSLVVGEQEGPIPRPCKQLFWKMCKILHLFYYQTDGFSSPKEMVSAVDAVIKEPLQLRS 829          
BLAST of FC877175 vs. ExPASy Swiss-Prot
Match: KSL5_ORYSI (Ent-isokaur-15-ene synthase OS=Oryza sativa subsp. indica GN=KSL5 PE=1 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 6.788e-16
Identity = 54/188 (28.72%), Postives = 87/188 (46.28%), Query Frame = 2
Query:   29 WYETFASWLTEATWSKSGRTPS-MEEYLETGMISIAAHTLVLTASCFLNPSLPNYKFRPAQYDTVTKLLMLVPRLLNDIQSYQKEIADGKQNSVILYL-------RENPEADIEDSVAYVRXXXXXXXXXXXXXXXMDGFSDLPRPCKQLHLFCMKAFQMFFHSSNRYDSNTEMIDDVQKAFYIPLKL 568
            W+ T    LTEA W      P+ +EEY+   + S A   ++ +A+ F+ P L    FR  +Y  +  L   + RLLND+Q+Y+KEI  GK NS++L+        R +PEA +E++   +R                DG   +P PC++L  F  K     +   + Y +   M+         PL++
Sbjct:  625 WWFTVRGMLTEAEWRMDKYVPTTVEEYMSAAVDSFAVGPIITSAALFVGPELSEEVFRSEEYIHLMNLANTIGRLLNDMQTYEKEIKMGKVNSIMLHALSHSGGGRGSPEASMEEAKREMRRVLQGSRCDLLRLVTRDG-GVVPPPCRKLFWFMSKVLHFVYMEKDGYFTADGMMASANAVILDPLQV 811          
BLAST of FC877175 vs. ExPASy Swiss-Prot
Match: KSL2_ORYSJ (Ent-kaurene synthase-like 2 OS=Oryza sativa subsp. japonica GN=KSL2 PE=2 SV=2)

HSP 1 Score: 84.3445 bits (207), Expect = 6.788e-16
Identity = 49/169 (28.99%), Postives = 81/169 (47.93%), Query Frame = 2
Query:   17 LIMKWYETFASWLTEATWSKSGRTPSMEEYLETGMISIAAHTLVLTASCFLNPSLPNYKFRPAQYDTVTKLLMLVPRLLNDIQSYQKEIADGKQNSV-ILYLRENPEADIEDSVAYVRXXXXXXXXXXXXXXXMDGFSDLPRPCKQLHLFCMKAFQMFFHSSNRYDSNT 520
            L+  W E   S +TE  W +S   P+ EEY+E  +++ A   +VL A   + P +P+   R  +   + +L+    RLLND+QSY++E + GK NSV +L L       +E++V  ++                 G   +PRPC++L     K    F+   + + S T
Sbjct:  532 LVEIWQELLRSMMTEVEWRQSRYVPTAEEYMENAVVTFALGPVVLPALYLVGPKMPDSVIRSQECSELFRLMSKCGRLLNDVQSYEREGSQGKLNSVSLLALHSGGSVSMEEAVKQIQRPIEKCRRELLKLVVSRG-GAVPRPCRELFWSMCKVCHFFYSGGDGFSSPT 699          
BLAST of FC877175 vs. ExPASy Swiss-Prot
Match: KSL7_ORYSJ (Ent-cassa-12,15-diene synthase OS=Oryza sativa subsp. japonica GN=KSL7 PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 8.866e-16
Identity = 49/187 (26.20%), Postives = 84/187 (44.92%), Query Frame = 2
Query:   17 LIMKWYETFASWLTEATWSKSGRTPSMEEYLETGMISIAAHTLVLTASCFLNPSLPNYKFRPAQYDTVTKLLMLVPRLLNDIQSYQKEIADGKQNSVILYL--RENPEADIEDSVAYVRXXXXXXXXXXXXXXXMDGFSDLPRPCKQLHLFCMKAFQMFFHSSNRYDSNTEMIDDVQKAFYIPLKLQ 571
            L+  W         EA W +S   P+ EEY+E+GM+S+     V++A   +   LP       +YD + +L+    RLLNDI+  ++E +DGK  + +  L         ++++   V                 +    +PRPCKQL     K   +F++ ++ + S  EM+  V      PL+L+
Sbjct:  641 LVDVWLHVVRCMKVEAEWQRSQHLPTFEEYMESGMVSLGQGCTVMSALFLIGEKLPEGIVELEEYDELFRLMGTCGRLLNDIRGIEREESDGKMTNGVSLLVHASGGSMSVDEAKTEVMKRIDASRRKLLSLVVSEQEGPIPRPCKQLFWKMCKILHLFYYQTDGFSSPKEMVSAVDAVINEPLQLR 827          
BLAST of FC877175 vs. ExPASy Swiss-Prot
Match: KSL5_ORYSJ (Ent-pimara-8(14),15-diene synthase OS=Oryza sativa subsp. japonica GN=KSL5 PE=1 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 1.512e-15
Identity = 55/188 (29.26%), Postives = 86/188 (45.74%), Query Frame = 2
Query:   29 WYETFASWLTEATWSKSGRTPS-MEEYLETGMISIAAHTLVLTASCFLNPSLPNYKFRPAQYDTVTKLLMLVPRLLNDIQSYQKEIADGKQNSVILYL-------RENPEADIEDSVAYVRXXXXXXXXXXXXXXXMDGFSDLPRPCKQLHLFCMKAFQMFFHSSNRYDSNTEMIDDVQKAFYIPLKL 568
            W+ T    LTEA W      P+ +EEY+   + S A    + +A+ F+ P L    FR  +Y  +  L   + RLLND+Q+Y+KEI  GK NSV+L+        R +PEA +E++   +R                DG   +P PC++L  F  K     +   + Y +   M+         PL++
Sbjct:  625 WWFTVRGMLTEAEWRMDKYVPTTVEEYMSAAVDSFALGPTITSAALFVGPELSEEVFRSKEYIHLMNLANTIGRLLNDMQTYEKEIKMGKVNSVMLHALSHSGGGRGSPEASMEEAKREMRRVLQGSRCDLLRLVTRDG-GVVPPPCRKLFWFMSKVLHFVYMEKDGYFTADGMMASANAVILDPLQV 811          
BLAST of FC877175 vs. ExPASy Swiss-Prot
Match: KSL3_ORYSJ (Ent-kaurene synthase-like 3 OS=Oryza sativa subsp. japonica GN=KSL3 PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 2.580e-15
Identity = 53/185 (28.65%), Postives = 84/185 (45.41%), Query Frame = 2
Query:   17 LIMKWYETFASWLTEATWSKSGRTPSMEEYLETGMISIAAHTLVLTASCFLNPSLPNYKFRPAQYDTVTKLLMLVPRLLNDIQSYQKEIADGKQNSV-ILYLRENPEADIEDSVAYVRXXXXXXXXXXXXXXXMDGFSDLPRPCKQLHLFCMKAFQMFFHSSNRYDSNTEMIDDVQKAFYIPLKL 568
            LI  W +   S +TE  W  S   P+ EEY+E   ++ A   +VL A   + P +P    R ++Y+ + +L+    RLLND+Q+Y++E  +GK NSV +L ++      IE++   +                  G S +P PCK+L     K    F+   + + S T     V      PL L
Sbjct:  540 LIEIWLDLLRSMMTEVEWRISNYVPTPEEYMENAAMTFALGPIVLPALYLVGPKIPESVVRDSEYNELFRLMSTCGRLLNDVQTYEREDGEGKVNSVSLLVIQSGGSVSIEEARREIMKPIERCRRELLGLVLRRG-SAVPGPCKELFWKMCKVCYFFYARGDGFSSPTAKSAAVDAVIRDPLDL 723          
The following BLAST results are available for this feature:
BLAST of FC877175 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 14
Match NameE-valueIdentityDescription
LIS_CLABR4.370e-3945.00S-linalool synthase OS=Clarkia breweri GN=LIS PE=2... [more]
KSB_ARATH7.749e-2030.32Ent-kaur-16-ene synthase, chloroplastic OS=Arabido... [more]
KS1_ORYSJ1.237e-1729.19Ent-kaur-16-ene synthase, chloroplastic OS=Oryza s... [more]
KSL6_ORYSJ5.198e-1628.80Ent-isokaur-15-ene synthase OS=Oryza sativa subsp.... [more]
KSL7_ORYSI6.788e-1626.06Ent-cassa-12,15-diene synthase OS=Oryza sativa sub... [more]
KSL5_ORYSI6.788e-1628.72Ent-isokaur-15-ene synthase OS=Oryza sativa subsp.... [more]
KSL2_ORYSJ6.788e-1628.99Ent-kaurene synthase-like 2 OS=Oryza sativa subsp.... [more]
KSL7_ORYSJ8.866e-1626.20Ent-cassa-12,15-diene synthase OS=Oryza sativa sub... [more]
KSL5_ORYSJ1.512e-1529.26Ent-pimara-8(14),15-diene synthase OS=Oryza sativa... [more]
KSL3_ORYSJ2.580e-1528.65Ent-kaurene synthase-like 3 OS=Oryza sativa subsp.... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAN3DE09RM1 EHR Citrus clementina cDNA clone KN0AAN3DE09, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC877175 ID=FC877175; Name=FC877175; organism=Citrus clementina; type=EST; length=708bp
GCACGAGGCATATGAATTAATTATGAAGTGGTATGAAACGTTTGCCTCGT
GGCTTACGGAAGCAACGTGGAGCAAAAGCGGCCGCACACCATCGATGGAA
GAGTACCTTGAAACTGGAATGATCTCAATTGCTGCACACACTCTAGTTCT
TACAGCTTCATGTTTCTTGAACCCAAGCTTGCCAAATTACAAATTCAGGC
CAGCGCAGTATGATACCGTAACCAAACTGCTAATGCTCGTTCCCCGTCTG
TTGAATGACATTCAGAGCTACCAGAAGGAAATAGCAGACGGGAAACAAAA
CTCCGTTATACTATACTTGAGGGAAAATCCTGAGGCAGACATTGAAGATT
CAGTTGCGTACGTGAGAGAGTTAATAGCCGCAAAGGAGAAAGAGCTGTTG
GAACATGCTCTAATGGATGGGTTCAGTGATCTGCCAAGACCATGCAAGCA
GCTCCACTTATTTTGTATGAAAGCATTTCAGATGTTCTTTCACTCTTCAA
ATCGATATGACTCCAACACTGAAATGATTGATGACGTTCAAAAGGCTTTT
TACATTCCCCTCAAACTTCAAACCCCCAAGCCGTTGATAAAGCTTATGCT
TAATCAGCTTCCCCTTCCCCTTCATCATCATTCTAGATCAAAGAATGATT
ACCAATCGGTCGCCAACTGTTTCAATATTTACGGCAAAAGAAGCCTTGCT
GCCCCGCG
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