FC878069

Overview
NameFC878069
Unique NameFC878069
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length897
Libraries
Library NameType
IVIA1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of FC878069 vs. ExPASy Swiss-Prot
Match: MFRN_DICDI (Mitoferrin OS=Dictyostelium discoideum GN=mcfF PE=3 SV=1)

HSP 1 Score: 162.155 bits (409), Expect = 3.794e-39
Identity = 80/197 (40.61%), Postives = 126/197 (63.96%), Query Frame = 1
Query:  241 DGLEFWQFMISGSIAGSVEHMAMYPVDTLKTRMQVI--GASRPLHPAGVRQAFSSVLKLEGPAGFYRGIAAMGLGAGPAHAVYFSVYELCK-EFFPGGVPNNSMAHAVSGVFSTVASDAVITPMDMVKQRLQLKSSPYKGVADCVKRVLVEEGIGAFYASYRTTVIMNAPFQAVHFATYEAVKRALMEFDPNSGSDE 822
            +G  F+  +I+G+ AG  EH  MYP+DT+KT +Q I  GA +        Q    +++  G  G +RG+ A+  GA P+HAV+FS+YEL K +F      ++ +   ++G  +T+ S+AV +PMD+VKQRLQL+ + YKG+ DC KR+ V+EGI  FY+ Y TT++MN P+  V+FA+YE++K+ +  +  N   +E
Sbjct:   12 EGGSFYVHLIAGAAAGFAEHCGMYPIDTIKTHIQAIKPGAMQ----TSSLQITKHIIQQHGITGLFRGLTAVAAGAAPSHAVHFSIYELLKFKFIGSDEDHHPIKVGIAGAIATMTSEAVASPMDVVKQRLQLQITDYKGLTDCTKRIWVKEGIRGFYSGYTTTLVMNVPYNIVYFASYESLKKIIQPWFNNKNPEE 204          
BLAST of FC878069 vs. ExPASy Swiss-Prot
Match: MFRN2_HUMAN (Mitoferrin-2 OS=Homo sapiens GN=SLC25A28 PE=2 SV=1)

HSP 1 Score: 152.91 bits (385), Expect = 2.302e-36
Identity = 83/207 (40.10%), Postives = 117/207 (56.52%), Query Frame = 1
Query:  166 PKYRTPDFHPEISVNPSKTKETTIHDGLEFWQFMISGSIAGSVEHMAMYPVDTLKTRMQVIGASRPLHPAGVRQAFSSVLKLEGPAGFYRGIAAMGLGAGPAHAVYFSVYELCKE-----FFPGGVPNNSMAHAVSGVFSTVASDAVITPMDMVKQRLQLKSSPYKGVADCVKRVLVEEGIGAFYASYRTTVIMNAPFQAVHFATYE 771
            P  + PD  P+    P+    TT          M++G++AG +EH  MYP+D +KTRMQ +          V +A   +++ EG     RG+     GAGPAHA+YF+ YE  K+       PGG  N+ +A+  +G  +T+  DA + P ++VKQR+Q+ +SPY  V DCV+ V   EG GAFY SY T + MN PFQA+HF TYE
Sbjct:   52 PVRQDPDSGPDYEALPAGATVTT---------HMVAGAVAGILEHCVMYPIDCVKTRMQSLQPDPAARYRNVLEALWRIIRTEGLWRPMRGLNVTATGAGPAHALYFACYEKLKKTLSDVIHPGG--NSHIANGAAGCVATLLHDAAMNPAEVVKQRMQMYNSPYHRVTDCVRAVWQNEGAGAFYRSYTTQLTMNVPFQAIHFMTYE 247          
BLAST of FC878069 vs. ExPASy Swiss-Prot
Match: MFRN2_MOUSE (Mitoferrin-2 OS=Mus musculus GN=Slc25a28 PE=2 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 5.128e-36
Identity = 80/201 (39.80%), Postives = 116/201 (57.71%), Query Frame = 1
Query:  187 FHPEISVNPSKTKE-TTIHDGLEFWQFMISGSIAGSVEHMAMYPVDTLKTRMQVIGASRPLHPAGVRQAFSSVLKLEGPAGFYRGIAAMGLGAGPAHAVYFSVYELCKE-----FFPGGVPNNSMAHAVSGVFSTVASDAVITPMDMVKQRLQLKSSPYKGVADCVKRVLVEEGIGAFYASYRTTVIMNAPFQAVHFATYE 771
            + P + ++P    E   +  G      M++G++AG +EH  MYP+D +KTRMQ +          V +A   +++ EG     RG+     GAGPAHA+YF+ YE  K+       PGG  N+ +A+  +G  +T+  DA + P ++VKQR+Q+ +SPY  V DCV+ V   EG GAFY SY T + MN PFQA+HF TYE
Sbjct:   49 YQPPVRLDPESGPEYEALPAGATVTTHMVAGAVAGILEHCVMYPIDCVKTRMQSLQPDPAARYRNVLEALWRIMRTEGLWRPMRGLNVTATGAGPAHALYFACYEKLKKTLSDVIHPGG--NSHIANGAAGCVATLLHDAAMNPAEVVKQRMQMYNSPYHRVTDCVRAVWQNEGAGAFYRSYTTQLTMNVPFQAIHFMTYE 247          
BLAST of FC878069 vs. ExPASy Swiss-Prot
Match: MFRN1_DANRE (Mitoferrin-1 OS=Danio rerio GN=slc25a37 PE=1 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 4.341e-35
Identity = 79/184 (42.93%), Postives = 112/184 (60.87%), Query Frame = 1
Query:  265 MISGSIAGSVEHMAMYPVDTLKTRMQVIGASRPLHPAGVRQAFSSVLKLEGPAGFYRGIAAMGLGAGPAHAVYFSVYELCKEFFPGGVPNNSMAHAVSGVFSTVAS---DAVITPMDMVKQRLQLKSSPYKGVADCVKRVLVEEGIGAFYASYRTTVIMNAPFQAVHFATYEAVKRALMEFDPN 807
            M +G++AG +EH  MYPVD++KTRMQ +          V  A   +++ EG     RG+    LGAGPAHA+YF+ YE  K      + N   +H  +GV  +VA+   DAV+ P ++VKQR+Q+ +SPY+ + DCV  V  +EG+ AFY SY T + MN PFQAVHF TYE ++     F+P+
Sbjct:   37 MTAGAVAGILEHTVMYPVDSVKTRMQSLQPDPKAQYRSVYGALKRIVRTEGLLRPLRGLNITVLGAGPAHALYFACYERIKRSLSDVIQNGGNSHIANGVAGSVATVLHDAVMNPAEVVKQRMQMYNSPYRSLYDCVLMVSRKEGLAAFYRSYSTQLTMNIPFQAVHFITYEFMQE---HFNPH 217          
BLAST of FC878069 vs. ExPASy Swiss-Prot
Match: MFRN_DROME (Mitoferrin OS=Drosophila melanogaster GN=mfrn PE=1 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 4.800e-34
Identity = 69/169 (40.83%), Postives = 107/169 (63.31%), Query Frame = 1
Query:  265 MISGSIAGSVEHMAMYPVDTLKTRMQVIGASRPLHPAGVRQAFSSVLKLEGPAGFYRGIAAMGLGAGPAHAVYFSVYELCKEFFPGGVPNNSMAHAVSGVFSTVASDAVITPMDMVKQRLQLKSSPYKGVADCVKRVLVEEGIGAFYASYRTTVIMNAPFQAVHFATYE 771
            M +G+IAG +EH+ MYP+D++KTRMQ +  S P     +     +++  EG     RG +A+ LGAGPAH++YF+ YE+ KE         ++ + +SG  +T+  DA+ +P D++KQR+Q+ +SPY  V  CV+ +   EG  AFY +Y T ++MN P+Q +HF TYE
Sbjct:   18 MTAGAIAGVLEHVVMYPLDSVKTRMQSL--SPPTKNMNIVSTLRTMITREGLLRPIRGASAVVLGAGPAHSLYFAAYEMTKELTAKFTSVRNLNYVISGAVATLIHDAISSPTDVIKQRMQMYNSPYTSVVSCVRDIYKREGFKAFYRAYGTQLVMNLPYQTIHFTTYE 184          
BLAST of FC878069 vs. ExPASy Swiss-Prot
Match: MFRN_CAEBR (Mitoferrin OS=Caenorhabditis briggsae GN=CBG02884 PE=3 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 1.824e-33
Identity = 77/187 (41.18%), Postives = 113/187 (60.43%), Query Frame = 1
Query:  265 MISGSIAGSVEHMAMYPVDTLKTRMQVIGASRPLHPAGVRQAFSSVLKLEGPAGFYRGIAAMGLGAGPAHAVYFSVYELCKEFFPGGVPNN--SMAHAVSGVFSTVASDAVITPMDMVKQRLQLKSSPYKGVADCVKRVLVEEGIGAFYASYRTTVIMNAPFQAVHFATYEAVKRAL---MEFDPNS 810
            + +G++AG+VEH  M+P D++KTRMQ +       P  V  +  S++K EG     RG+ A+  G+ PAHA+YF+VYE  K F  G    +  ++A+  SGV +T+  DAV+ P ++VKQR+Q+  SPY    +C + V   EG  AFY SY T + MN PFQA+HF  YE  ++ L    ++DP S
Sbjct:   21 LAAGALAGAVEHCVMFPFDSVKTRMQSLCPCETKCPTPVH-SLMSIVKREGWLRPLRGVNAVAAGSMPAHALYFTVYEKMKSFLTGNTAGHEHTLAYGASGVVATLIHDAVMNPAEVVKQRMQMAYSPYGSSLECARCVYNREGFAAFYRSYTTQLAMNVPFQAIHFMGYEFWQQVLNPEHKYDPKS 206          
BLAST of FC878069 vs. ExPASy Swiss-Prot
Match: MFRN_CAEEL (Mitoferrin OS=Caenorhabditis elegans GN=W02B12.9 PE=2 SV=1)

HSP 1 Score: 141.739 bits (356), Expect = 5.307e-33
Identity = 74/187 (39.57%), Postives = 113/187 (60.43%), Query Frame = 1
Query:  265 MISGSIAGSVEHMAMYPVDTLKTRMQVIGASRPLHPAGVRQAFSSVLKLEGPAGFYRGIAAMGLGAGPAHAVYFSVYELCKEFFPGGVP--NNSMAHAVSGVFSTVASDAVITPMDMVKQRLQLKSSPYKGVADCVKRVLVEEGIGAFYASYRTTVIMNAPFQAVHFATYEAVKRAL---MEFDPNS 810
            + +G++AG+VEH  M+P D++KTRMQ +             +  S++K EG     RG+ A+  G+ PAHA+YF+VYE  K +  G     +N++A+  SGV +T+  DA++ P ++VKQR+Q+  SPY    +C + V   EG+ AFY SY T + MN PFQA+HF +YE  +  L    ++DP S
Sbjct:   21 LTAGALAGAVEHCVMFPFDSVKTRMQSLCPCPETKCPTPVHSLMSIVKREGWLRPLRGVNAVAAGSMPAHALYFTVYEKMKGYLTGNSAGHSNTLAYGASGVVATLIHDAIMNPAEVVKQRMQMAFSPYGSSLECARCVYNREGVAAFYRSYTTQLAMNVPFQAIHFMSYEFWQHVLNPEHKYDPKS 207          
BLAST of FC878069 vs. ExPASy Swiss-Prot
Match: MFRN2_DANRE (Mitoferrin-2 OS=Danio rerio GN=slc25a28 PE=2 SV=1)

HSP 1 Score: 141.739 bits (356), Expect = 5.307e-33
Identity = 75/190 (39.47%), Postives = 114/190 (60.00%), Query Frame = 1
Query:  265 MISGSIAGSVEHMAMYPVDTLKTRMQVIGASRPLHPAGVRQAFSSVLKLEGPAGFYRGIAAMGLGAGPAHAVYFSVYELCKEFF-----PGGVPNNSMAHAVSGVFSTVASDAVITPMDMVKQRLQLKSSPYKGVADCVKRVLVEEGIGAFYASYRTTVIMNAPFQAVHFATYEAVKRAL---MEFDPNS 810
            M++G++AG +EH  M+P+D +KTRMQ +          V  A   +++ EG     RG+    +GAGPAHA+YF+ YE  K+       PG   N+ +A+  +G  +T+  DA + P ++VKQR+Q+ +SPY+ V DC++ V   EG  AFY SY T + MN PFQA+HF TYE ++  L     ++P+S
Sbjct:   81 MLAGAVAGIMEHCLMFPIDCVKTRMQSLQPEPAARYRNVMDALWRIMRTEGIWRPIRGLNITAVGAGPAHALYFACYERLKKVLSDIIHPGA--NSHLANGAAGCVATLLHDAAMNPTEVVKQRMQMYNSPYRSVLDCMRCVWQREGALAFYRSYTTQLTMNVPFQALHFMTYEYLQELLNPQRHYNPSS 268          
BLAST of FC878069 vs. ExPASy Swiss-Prot
Match: MFRN1_HUMAN (Mitoferrin-1 OS=Homo sapiens GN=SLC25A37 PE=1 SV=2)

HSP 1 Score: 140.969 bits (354), Expect = 9.052e-33
Identity = 72/174 (41.38%), Postives = 107/174 (61.49%), Query Frame = 1
Query:  265 MISGSIAGSVEHMAMYPVDTLKTRMQVIGASRPLHPAGVRQAFSSVLKLEGPAGFYRGIAAMGLGAGPAHAVYFSVYELCKE-----FFPGGVPNNSMAHAVSGVFSTVASDAVITPMDMVKQRLQLKSSPYKGVADCVKRVLVEEGIGAFYASYRTTVIMNAPFQAVHFATYE 771
            M +G++AG +EH  MYPVD++KTRMQ +          +  A   +++ EG     RG+  M +GAGPAHA+YF+ YE  K      F   G  N+ +A+ ++G  +T+  DAV+ P ++VKQRLQ+ +S ++    C++ V   EG+GAFY SY T + MN PFQ++HF TYE
Sbjct:   49 MTAGAMAGILEHSVMYPVDSVKTRMQSLSPDPKAQYTSIYGALKKIMRTEGFWRPLRGVNVMIMGAGPAHAMYFACYENMKRTLNDVFHHQG--NSHLANGIAGSMATLLHDAVMNPAEVVKQRLQMYNSQHRSAISCIRTVWRTEGLGAFYRSYTTQLTMNIPFQSIHFITYE 220          
BLAST of FC878069 vs. ExPASy Swiss-Prot
Match: YETC_SCHPO (Uncharacterized mitochondrial carrier C8C9.12c OS=Schizosaccharomyces pombe GN=SPAC8C9.12c PE=2 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 2.634e-32
Identity = 79/196 (40.31%), Postives = 114/196 (58.16%), Query Frame = 1
Query:  244 GLEFWQFMISGSIAGSVEHMAMYPVDTLKTRMQVI-GASRPLHPAGVRQAFSSVLKLEGPAGFY---RGIAAMGLGAGPAHAVYFSVYELCKEFFPGGVPNNSMAHAVSGVFSTVASDAVITPMDMVKQRLQLKSSPYKGVADCVKRVLVEEGIGAFYASYRTTVIMNAPFQAVHFATYEAVKRAL---MEFDPNS 810
            G   +  +++G+ +G +EH  MYPVD +KTRMQ++ G SR +    V    +SV+K+    G Y   RGI+++ +GAGP+HA+YFSV E  K       P+  +A A++G  +   SDA +TP D++KQR+QL S  YK    C   V   EG+GAFY SY T + M+ PF A+  ATY+     L     +DP S
Sbjct:   16 GSPMYAHLLAGAFSGILEHSVMYPVDAIKTRMQMLNGVSRSVSGNIV----NSVIKISSTEGVYSLWRGISSVIMGAGPSHAIYFSVLEFFKSKINAS-PDRPLASALAGACAITISDAFMTPFDVIKQRMQLPSRKYKSALHCATTVFRNEGLGAFYISYPTCIAMSIPFTAIQVATYDTCMSFLNPNAVYDPTS 206          

HSP 2 Score: 69.3218 bits (168), Expect = 3.347e-11
Identity = 52/188 (27.66%), Postives = 84/188 (44.68%), Query Frame = 1
Query:  268 ISGSIAGSVEHMAMYPVDTLKTRMQVIGASRPLHPAGVRQAFSSVLKLEGPAGFYRGIAAMGLGAGPAHAVYFSVYELCKEFFPGGVPNNSMAHAVSGVFSTVASDAVITPMDMVKQRLQLKSS-------PYKGVADCVKRVLVEEGIGAFYASYRTTVIMNAPFQAVHFATYEAVKRALMEFDPNS 810
            ++G+ A ++    M P D +K RMQ+   SR    A      ++V + EG   FY         + P  A+  + Y+ C  F       +  +H +SG  S   + ++ TP+D+VK  LQ + S         KG  D V+ +    GI +F+   R  +++  P  AV +A YEA K  L+     S
Sbjct:  118 LAGACAITISDAFMTPFDVIKQRMQL--PSRKYKSA--LHCATTVFRNEGLGAFYISYPTCIAMSIPFTAIQVATYDTCMSFLNPNAVYDPTSHIISGGLSGAIASSLTTPLDVVKTLLQTRGSSSIPEVRKCKGSLDVVRFIYNYGGIPSFFKGIRPRMVVAMPATAVSWAAYEAGKEILIRVSKTS 301          
The following BLAST results are available for this feature:
BLAST of FC878069 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 164
Match NameE-valueIdentityDescription
MFRN_DICDI3.794e-3940.61Mitoferrin OS=Dictyostelium discoideum GN=mcfF PE=... [more]
MFRN2_HUMAN2.302e-3640.10Mitoferrin-2 OS=Homo sapiens GN=SLC25A28 PE=2 SV=1[more]
MFRN2_MOUSE5.128e-3639.80Mitoferrin-2 OS=Mus musculus GN=Slc25a28 PE=2 SV=1[more]
MFRN1_DANRE4.341e-3542.93Mitoferrin-1 OS=Danio rerio GN=slc25a37 PE=1 SV=1[more]
MFRN_DROME4.800e-3440.83Mitoferrin OS=Drosophila melanogaster GN=mfrn PE=1... [more]
MFRN_CAEBR1.824e-3341.18Mitoferrin OS=Caenorhabditis briggsae GN=CBG02884 ... [more]
MFRN_CAEEL5.307e-3339.57Mitoferrin OS=Caenorhabditis elegans GN=W02B12.9 P... [more]
MFRN2_DANRE5.307e-3339.47Mitoferrin-2 OS=Danio rerio GN=slc25a28 PE=2 SV=1[more]
MFRN1_HUMAN9.052e-3341.38Mitoferrin-1 OS=Homo sapiens GN=SLC25A37 PE=1 SV=2[more]
YETC_SCHPO2.634e-3240.31Uncharacterized mitochondrial carrier C8C9.12c OS=... [more]

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Properties
Property NameValue
Genbank descriptionIC0AAA69AF09RM1 IVIA1 Citrus clementina cDNA clone IC0AAA69AF09, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC878069 ID=FC878069; Name=FC878069; organism=Citrus clementina; type=EST; length=897bp
TAAAATTATCAGCCAAAAATAATTCAGTGCATGGCGACAAAGTCACCTCC
ATTCGACCCGAAAACGATCTCCAATCACCAAATCCTCCACTCCTATTAAA
CCTTAACAGCACAAGCAACTTCTCGTTATACAAACTTCACCAGTACTATG
GCTACTGACGCCTCCCCCAAATATCGGACCCCCGATTTCCACCCCGAAAT
CTCGGTAAACCCCTCCAAAACAAAAGAAACAACAATACACGACGGCCTCG
AGTTCTGGCAGTTCATGATTTCCGGGTCCATCGCGGGCTCCGTTGAGCAC
ATGGCCATGTACCCAGTTGACACCCTCAAGACCCGCATGCAAGTCATAGG
GGCGTCTCGCCCGCTTCATCCCGCCGGCGTCCGTCAAGCCTTTAGCTCAG
TGCTCAAGCTGGAGGGCCCGGCAGGGTTTTACCGAGGAATTGCAGCTATG
GGACTCGGCGCAGGACCCGCACACGCTGTTTACTTCTCGGTTTACGAGCT
CTGTAAAGAGTTCTTCCCAGGTGGGGTCCCTAATAACTCAATGGCTCACG
CTGTGTCGGGTGTCTTCTCGACGGTGGCGAGTGATGCTGTGATTACGCCC
ATGGATATGGTGAAGCAGAGGCTTCAGCTCAAGAGCAGTCCGTACAAGGG
AGTCGCAGACTGTGTCAAGAGAGTGTTAGTTGAGGAAGGAATCGGCGCGT
TTTACGCTTCATATAGGACTACGGTGATCATGAACGCGCCGTTTCAAGCA
GTTCATTTCGCCACTTATGAGGCAGTAAAGAGAGCTTTGATGGAATTTGA
CCCAAACAGTGGGAGTGATGAGAGTTTAGTGGTTCATGCAACAGCCGGTG
CGGCTGCCGGAGCTCTGGCCGCTACCTTGACGACGCCACTTGATGTT
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