FC923688

Overview
NameFC923688
Unique NameFC923688
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length686
Libraries
Library NameType
StrClemenNcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of FC923688 vs. ExPASy Swiss-Prot
Match: P5CS_SOLLC (Delta-1-pyrroline-5-carboxylate synthetase OS=Solanum lycopersicum GN=PRO2 PE=2 SV=1)

HSP 1 Score: 338.191 bits (866), Expect = 2.459e-92
Identity = 170/225 (75.56%), Postives = 187/225 (83.11%), Query Frame = 2
Query:    2 LHKVITSAIPDTVGEKLIGLVTSREEIPXXXXXXXXXXXXXPRGSNKLVSQIKESTKIPVLGHADGICHVYVDKSANLDVAKNIVIDAKIDYPAACNAMETLLVHKDLACNGALNELVVELQHEGVGLFGGPRASKLLKIPETRLFHHEYNSMVCTVEIVDDVRAAIDHIHQHGSAHTDCIVAEDXKVAETFLCQVDSAAVFHNASTRFCDGSTIGLGAEVGNST 676
            LHKVITSAIP +VGE+LIGLVTSREEIP             PRGSNKLVSQIK STKIPVLGHADGICHVYVDKSA++D+AK I +DAKIDYPAACNAMETLLVHKDLA NG LN+L+VELQ +GV L+GGP+AS LL IPE R F HEY+S+ CTVE+V+DV AAIDHIHQHGSAHTD I+ ED +VAE FL QVDSAAVFHNASTRF DG   GLGAEVG ST
Sbjct:  448 LHKVITSAIPVSVGERLIGLVTSREEIPELLKLDDVIDLVIPRGSNKLVSQIKASTKIPVLGHADGICHVYVDKSADMDMAKRITVDAKIDYPAACNAMETLLVHKDLAQNGGLNDLIVELQTKGVSLYGGPKASSLLMIPEARTFRHEYSSLACTVEVVEDVYAAIDHIHQHGSAHTDSIITEDQEVAEVFLRQVDSAAVFHNASTRFSDGFRFGLGAEVGIST 672          
BLAST of FC923688 vs. ExPASy Swiss-Prot
Match: P5CS_ACTDE (Delta-1-pyrroline-5-carboxylate synthetase OS=Actinidia deliciosa PE=2 SV=1)

HSP 1 Score: 332.413 bits (851), Expect = 1.349e-90
Identity = 166/226 (73.45%), Postives = 186/226 (82.30%), Query Frame = 2
Query:    2 LHKVITSAIPDTVGEKLIGLVTSREEIPXXXXXXXXXXXXXPRGSNKLVSQIKESTKIPVLGHADGICHVYVDKSANLDVAKNIVIDAKIDYPAACNAMETLLVHKDLACNGALNELVVELQHEGVGLFGGPRASKLLKIPETRLFHHEYNSMVCTVEIVDDVRAAIDHIHQHGSAHTDCIVAEDXKVAETFLCQVDSAAVFHNASTRFCDGSTIGLGAEVGNSTA 679
            LHKVITSAIP+ VG +LIGLVTSREEIP             PRGSNKLVSQIKESTKIPVLGHADGICHVYVDKSAN+D+AK +V+DAK DYPAACNAMETLLVHKDL  NG L+EL+VELQ +GV + GGPRAS LL IPE R  HHEY+S+ CT+EIVDDV AAIDHIH+HGSAHTD I+ ED +VAE FL QVDS++V HNASTRF DG+  GLGAEVG ST+
Sbjct:  448 LHKVITSAIPENVGPRLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHADGICHVYVDKSANMDMAKKVVLDAKTDYPAACNAMETLLVHKDLVQNGCLDELIVELQIKGVVIHGGPRASSLLHIPEARSLHHEYSSLACTIEIVDDVYAAIDHIHRHGSAHTDSIITEDHEVAEIFLRQVDSSSVLHNASTRFSDGARFGLGAEVGISTS 673          
BLAST of FC923688 vs. ExPASy Swiss-Prot
Match: P5CS2_ARATH (Delta-1-pyrroline-5-carboxylate synthetase B OS=Arabidopsis thaliana GN=P5CSB PE=2 SV=1)

HSP 1 Score: 330.872 bits (847), Expect = 3.925e-90
Identity = 169/226 (74.78%), Postives = 182/226 (80.53%), Query Frame = 2
Query:    2 LHKVITSAIPDTVGEKLIGLVTSREEIPXXXXXXXXXXXXXPRGSNKLVSQIKESTKIPVLGHADGICHVYVDKSANLDVAKNIVIDAKIDYPAACNAMETLLVHKDLACNGALNELVVELQHEGVGLFGGPRASKLLKIPETRLFHHEYNSMVCTVEIVDDVRAAIDHIHQHGSAHTDCIVAEDXKVAETFLCQVDSAAVFHNASTRFCDGSTIGLGAEVGNSTA 679
            LHKVIT AIP+TVG KLIGLVTSREEIP             PRGSNKLVSQIK STKIPVLGHADGICHVYVDKS  LD+AK IV DAK+DYPAACNAMETLLVHKDL  NG L++L+  LQ +GV L+GGPRAS  L IPET+ FHHEY+S  CTVEIV+DV  AIDHIHQHGSAHTDCIV ED +VAE FL QVDSAAVFHNASTRF DG   GLGAEVG ST+
Sbjct:  448 LHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKNSTKIPVLGHADGICHVYVDKSGKLDMAKRIVSDAKLDYPAACNAMETLLVHKDLEQNGFLDDLIYVLQTKGVTLYGGPRASAKLNIPETKSFHHEYSSKACTVEIVEDVYGAIDHIHQHGSAHTDCIVTEDSEVAEIFLRQVDSAAVFHNASTRFSDGFRFGLGAEVGISTS 673          
BLAST of FC923688 vs. ExPASy Swiss-Prot
Match: P5CS_MESCR (Delta-1-pyrroline-5-carboxylate synthetase OS=Mesembryanthemum crystallinum GN=P5CS PE=2 SV=1)

HSP 1 Score: 327.02 bits (837), Expect = 5.668e-89
Identity = 168/228 (73.68%), Postives = 185/228 (81.14%), Query Frame = 2
Query:    2 LHKVITSAIPDTVGEKLIGLVTSREEIPXXXXXXXXXXXXXPRGSNKLVSQIKESTKIPVLGHADGICHVYVDKSANLDVAKNIVIDAKIDYPAACNAMETLLVHKDLACNGALNELVVELQHEGVGLFGGPRASKL--LKIPETRLFHHEYNSMVCTVEIVDDVRAAIDHIHQHGSAHTDCIVAEDXKVAETFLCQVDSAAVFHNASTRFCDGSTIGLGAEVGNSTA 679
            LHKVITSAIPD VGEKLIGLVTSR+EIP             PRGSNKLVSQIKEST+IPVLGHADGICHVYVDKSAN+D+AK IV+DAK DYPAACNAMETLLVHKDLA NG LN+L+V+L+ EGV +FGGPR   L    I  T+ F+ EY+S  CTVEIVDDV AAI+HI+ HGSAHTDCI+AED KVAETFL  VDSAAV HNASTRFCDG   GLGAEVG ST+
Sbjct:  445 LHKVITSAIPDKVGEKLIGLVTSRDEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTRIPVLGHADGICHVYVDKSANMDMAKRIVLDAKTDYPAACNAMETLLVHKDLAENGGLNDLIVDLRTEGVTMFGGPRIDALQEFNIQATQTFNREYSSPACTVEIVDDVYAAIEHINHHGSAHTDCIIAEDHKVAETFLQLVDSAAVLHNASTRFCDGFRFGLGAEVGISTS 672          
BLAST of FC923688 vs. ExPASy Swiss-Prot
Match: P5CS1_ARATH (Delta-1-pyrroline-5-carboxylate synthetase A OS=Arabidopsis thaliana GN=P5CSA PE=1 SV=1)

HSP 1 Score: 323.168 bits (827), Expect = 8.184e-88
Identity = 163/225 (72.44%), Postives = 179/225 (79.56%), Query Frame = 2
Query:    2 LHKVITSAIPDTVGEKLIGLVTSREEIPXXXXXXXXXXXXXPRGSNKLVSQIKESTKIPVLGHADGICHVYVDKSANLDVAKNIVIDAKIDYPAACNAMETLLVHKDLACNGALNELVVELQHEGVGLFGGPRASKLLKIPETRLFHHEYNSMVCTVEIVDDVRAAIDHIHQHGSAHTDCIVAEDXKVAETFLCQVDSAAVFHNASTRFCDGSTIGLGAEVGNST 676
            LHKVIT AIP+TVG KLIGLVTSREEIP             PRGSNKLV+QIK +TKIPVLGHADGICHVYVDK+ + D+AK IV DAK+DYPAACNAMETLLVHKDL  N  LNEL+  LQ  GV L+GGPRASK+L IPE R F+HEY +  CTVE+V+DV  AIDHIH+HGSAHTDCIV ED +VAE FL QVDSAAVFHNASTRF DG   GLGAEVG ST
Sbjct:  448 LHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVTQIKNTTKIPVLGHADGICHVYVDKACDTDMAKRIVSDAKLDYPAACNAMETLLVHKDLEQNAVLNELIFALQSNGVTLYGGPRASKILNIPEARSFNHEYCAKACTVEVVEDVYGAIDHIHRHGSAHTDCIVTEDHEVAELFLRQVDSAAVFHNASTRFSDGFRFGLGAEVGVST 672          
BLAST of FC923688 vs. ExPASy Swiss-Prot
Match: P5CS_ORYSJ (Delta-1-pyrroline-5-carboxylate synthetase OS=Oryza sativa subsp. japonica GN=P5CS PE=2 SV=2)

HSP 1 Score: 314.694 bits (805), Expect = 2.911e-85
Identity = 158/225 (70.22%), Postives = 182/225 (80.89%), Query Frame = 2
Query:    2 LHKVITSAIPDTVGEKLIGLVTSREEIPXXXXXXXXXXXXXPRGSNKLVSQIKESTKIPVLGHADGICHVYVDKSANLDVAKNIVIDAKIDYPAACNAMETLLVHKDLACNGALNELVVELQHEGVGLFGGPRASKLLKIPETRLFHHEYNSMVCTVEIVDDVRAAIDHIHQHGSAHTDCIVAEDXKVAETFLCQVDSAAVFHNASTRFCDGSTIGLGAEVGNST 676
            LHKVIT AIP  VGEKLIGLVT+R+EI              PRGSNKLVSQIK STKIPVLGHADGICHVY+DKSA++D+AK+IV+DAKIDYPAACNAMETLLVHKDL  +  L++++V L+ EGV ++GGP A K L  P+   FHHEY+SM CTVE VDDV++AIDHIH++GSAHTDCIV  D KVAETFL +VDSAAVFHNASTRF DG+  GLGAEVG ST
Sbjct:  448 LHKVITDAIPRNVGEKLIGLVTTRDEIADLLKLDDVIDLVIPRGSNKLVSQIKASTKIPVLGHADGICHVYIDKSADMDMAKHIVMDAKIDYPAACNAMETLLVHKDLMKSPGLDDILVALKTEGVNIYGGPIAHKALGFPKAVSFHHEYSSMACTVEFVDDVQSAIDHIHRYGSAHTDCIVTTDDKVAETFLRRVDSAAVFHNASTRFSDGARFGLGAEVGIST 672          
BLAST of FC923688 vs. ExPASy Swiss-Prot
Match: P5CS_VIGAC (Delta-1-pyrroline-5-carboxylate synthetase OS=Vigna aconitifolia PE=2 SV=1)

HSP 1 Score: 301.597 bits (771), Expect = 2.550e-81
Identity = 152/213 (71.36%), Postives = 171/213 (80.28%), Query Frame = 2
Query:    2 LHKVITSAIPDTVGEKLIGLVTSREEIPXXXXXXXXXXXXXPRGSNKLVSQIKESTKIPVLGHADGICHVYVDKSANLDVAKNIVIDAKIDYPAACNAMETLLVHKDLACNGALNELVVELQHEGVGLFGGPRASKLLKIPETRLFHHEYNSMVCTVEIVDDVRAAIDHIHQHGSAHTDCIVAEDXKVAETFLCQVDSAAVFHNASTRFCDGS 640
            LHKVI  AIPD VG KLIGLVTSREEIP             PRGSNKLVSQIK STKIPVLGHADGICHVYVDKSAN+++AK IV+DAK+DYPAACNAMETLL+HKDL   G L E++++L+ EGV L+GGP AS LL IP+   FHHEY+S+ CT EIVDDV AAIDHI+ +GSAHTD IVAED +VA  FL QVDSAAVFHNASTRF DG+
Sbjct:  449 LHKVIIEAIPDNVGGKLIGLVTSREEIPELLKLDDVIDLVIPRGSNKLVSQIKSSTKIPVLGHADGICHVYVDKSANVEMAKRIVLDAKVDYPAACNAMETLLIHKDLIEKGWLKEIILDLRTEGVILYGGPVASSLLNIPQAHSFHHEYSSLACTAEIVDDVYAAIDHINLYGSAHTDSIVAEDNEVANVFLRQVDSAAVFHNASTRFSDGA 661          
BLAST of FC923688 vs. ExPASy Swiss-Prot
Match: PROA_SYNY3 (Probable gamma-glutamyl phosphate reductase OS=Synechocystis sp. (strain PCC 6803) GN=proA PE=3 SV=1)

HSP 1 Score: 193.741 bits (491), Expect = 7.489e-49
Identity = 111/228 (48.68%), Postives = 145/228 (63.60%), Query Frame = 2
Query:    2 LHKVITSAIPDTV-GEKLIGLVTSREEIPXXXXXXXXXXXXXPRGSNKLVSQIKESTKIPVLGHADGICHVYVDKSANLDVAKNIVIDAKIDYPAACNAMETLLVHKDLACNGALNELVVELQHEGVGLFGGPRASKLLKI-PETRL-FHHEYNSMVCTVEIVDDVRAAIDHIHQHGSAHTDCIVAEDXKVAETFLCQVDSAAVFHNASTRFCDGSTIGLGAEVGNST 676
            L +VI +A+  TV   + I L+T+REEI              PRGSN+ V  I+++T+IPVLGHADGICH+Y+D  A+L  A  I +DAK  YPAACNA+ETLLVH+ +A    L  L   L  +GV L G     KL+   P T   +  EY+ ++ +++IVD + AAIDHI+Q+GS HTD I++ED   AE F  +VDSA V+HN STRF DG   G GAEVG ST
Sbjct:  163 LTEVIQTALAKTVVSPEAINLLTTREEIRELLGLNQYVDLIIPRGSNEFVQYIQQNTQIPVLGHADGICHLYLDAQADLSKAIPITVDAKTQYPAACNAIETLLVHQAIAAE-FLPPLAQALGEKGVSLRGDSGTQKLIDCEPATEADWCTEYSDLILSIKIVDSLEAAIDHINQYGSKHTDGIISEDLTAAEQFCQRVDSAGVYHNCSTRFADGFRYGFGAEVGIST 389          
BLAST of FC923688 vs. ExPASy Swiss-Prot
Match: P5CS_HUMAN (Delta-1-pyrroline-5-carboxylate synthetase OS=Homo sapiens GN=ALDH18A1 PE=1 SV=2)

HSP 1 Score: 191.43 bits (485), Expect = 3.717e-48
Identity = 102/229 (44.54%), Postives = 144/229 (62.88%), Query Frame = 2
Query:    2 LHKVITSAIPDTVGEKLIGLVTSREEIPXXXXXXXXXXXXXPRGSNKLVSQIKESTK-IPVLGHADGICHVYVDKSANLDVAKNIVIDAKIDYPAACNAMETLLVHKDLACNGALNELVVELQHEGVGLFGGPRASKLLKIP--ETRLFHHEYNSMVCTVEIVDDVRAAIDHIHQHGSAHTDCIVAEDXKVAETFLCQVDSAAVFHNASTRFCDGSTIGLGAEVGNSTA 679
            LH +   A+     ++ + LV +REE+              PRGS++LV  I+++ K IPV+GH++GICH+YVD  A++D    +V D+K +YPAACNA+ETLL+H+DL      ++++  L+ E V +  GP+ +  L     E +    EY  +   +E+VD+V+ AIDHIH++GS+HTD IV ED   AE FL  VDSA VF NASTRF DG   GLGAEVG ST+
Sbjct:  516 LHLLTQEALSIHGVKEAVQLVNTREEVEDLCRLDKMIDLIIPRGSSQLVRDIQKAAKGIPVMGHSEGICHMYVDSEASVDKVTRLVRDSKCEYPAACNALETLLIHRDLLRTPLFDQIIDMLRVEQVKIHAGPKFASYLTFSPSEVKSLRTEYGDLELCIEVVDNVQDAIDHIHKYGSSHTDVIVTEDENTAEFFLQHVDSACVFWNASTRFSDGYRFGLGAEVGISTS 744          
BLAST of FC923688 vs. ExPASy Swiss-Prot
Match: ALH13_CAEEL (Probable delta-1-pyrroline-5-carboxylate synthetase OS=Caenorhabditis elegans GN=alh-13 PE=2 SV=1)

HSP 1 Score: 189.889 bits (481), Expect = 1.081e-47
Identity = 107/231 (46.32%), Postives = 141/231 (61.04%), Query Frame = 2
Query:    2 LHKVITSAIPDTVGEKL---IGLVTSREEIPXXXXXXXXXXXXXPRGSNKLVSQIKESTK-IPVLGHADGICHVYVDKSANLDVAKNIVIDAKIDYPAACNAMETLLVHKDLACNGALNELVVELQHEGVGLFGGPRASKLLKI--PETRLFHHEYNSMVCTVEIVDDVRAAIDHIHQHGSAHTDCIVAEDXKVAETFLCQVDSAAVFHNASTRFCDGSTIGLGAEVGNST 676
            LH ++  A+  T G ++   + LV SRE++              PRGS+ LV  ++E +K IPVLGHA+G+ HVY+DK  +   A  IV D+K DYP+ACNA ET+L+HKDLA     + L    + EGV L  GP+ + LLK   P       EY S+ CT+E+VD+V  A+ HI ++GS HT+ I+ E+   AE FL  VDSA  FHNASTRF DG   GLGAEVG ST
Sbjct:  508 LHALVQEAL-GTHGFEMRDAVTLVRSREDVADLLQLKDLIDLIIPRGSSDLVRSMQEKSKGIPVLGHAEGVWHVYIDKDCDEQKAIQIVRDSKCDYPSACNAAETILIHKDLATAPFFDSLCSMFKAEGVKLHAGPKLAALLKFAPPPAESMSFEYGSLECTLEVVDNVEEAVAHIIRYGSGHTESIITENTNTAEHFLKHVDSACAFHNASTRFADGYRFGLGAEVGIST 737          
The following BLAST results are available for this feature:
BLAST of FC923688 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
P5CS_SOLLC2.459e-9275.56Delta-1-pyrroline-5-carboxylate synthetase OS=Sola... [more]
P5CS_ACTDE1.349e-9073.45Delta-1-pyrroline-5-carboxylate synthetase OS=Acti... [more]
P5CS2_ARATH3.925e-9074.78Delta-1-pyrroline-5-carboxylate synthetase B OS=Ar... [more]
P5CS_MESCR5.668e-8973.68Delta-1-pyrroline-5-carboxylate synthetase OS=Mese... [more]
P5CS1_ARATH8.184e-8872.44Delta-1-pyrroline-5-carboxylate synthetase A OS=Ar... [more]
P5CS_ORYSJ2.911e-8570.22Delta-1-pyrroline-5-carboxylate synthetase OS=Oryz... [more]
P5CS_VIGAC2.550e-8171.36Delta-1-pyrroline-5-carboxylate synthetase OS=Vign... [more]
PROA_SYNY37.489e-4948.68Probable gamma-glutamyl phosphate reductase OS=Syn... [more]
P5CS_HUMAN3.717e-4844.54Delta-1-pyrroline-5-carboxylate synthetase OS=Homo... [more]
ALH13_CAEEL1.081e-4746.32Probable delta-1-pyrroline-5-carboxylate synthetas... [more]

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Properties
Property NameValue
Genbank descriptionC31707H06EF StrClemenN Citrus clementina cDNA clone C31707H06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC923688 ID=FC923688; Name=FC923688; organism=Citrus clementina; type=EST; length=686bp
CTTGCACAAGGTCATCACCTCAGCTATCCCAGATACTGTTGGTGAGAAAC
TTATTGGACTTGTGACTTCCAGGGAAGAGATTCCTGATTTGCTCAAGCTT
GATGATGTGATAGATCTTGTTATCCCCAGAGGCAGCAATAAGCTGGTTTC
TCAAATCAAGGAGTCAACAAAAATTCCTGTACTTGGTCATGCTGATGGAA
TTTGTCATGTTTATGTTGATAAATCGGCTAACCTTGATGTGGCAAAGAAC
ATTGTTATTGATGCGAAGATAGACTATCCTGCAGCCTGCAATGCTATGGA
AACTCTTCTGGTACACAAGGATTTAGCATGCAATGGTGCACTCAATGAGC
TTGTTGTAGAACTTCAACATGAAGGTGTCGGTTTATTTGGCGGACCAAGA
GCAAGTAAATTACTAAAAATTCCTGAGACACGCTTATTCCATCATGAGTA
CAACTCAATGGTTTGTACGGTTGAAATTGTGGACGATGTGCGTGCTGCTA
TTGACCATATTCATCAACATGGAAGTGCCCATACTGATTGCATTGTTGCA
GAAGATNCAAAAGTTGCTGAAACATTTTTATGTCAAGTTGACAGTGCTGC
AGTATTTCATAATGCAAGTACACGATTCTGTGATGGAAGCACGATTGGAC
TTGGTGCAGAGGTTGGAAATAGCACAGCAGATTCAT
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