FC931332

Overview
NameFC931332
Unique NameFC931332
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length743
Libraries
Library NameType
PostHarvP1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of FC931332 vs. ExPASy Swiss-Prot
Match: RAB1B_RAT (Ras-related protein Rab-1B OS=Rattus norvegicus GN=Rab1b PE=1 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 9.717e-19
Identity = 24/60 (40.00%), Postives = 41/60 (68.33%), Query Frame = 1 
Query:  319 YKLVFLGDQSVGKTSIITRFMYDKFDNTYQATIGIDFLSKTMYLEDRTVRLQLWTREIQE 498
            +KL+ +GD  VGK+ ++ RF  D +  +Y +TIG+DF  +T+ L+ +T++LQ+W    QE
Sbjct:    9 FKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQE 68

HSP 2 Score: 56.225 bits (134), Expect = 9.717e-19
Identity = 26/65 (40.00%), Postives = 42/65 (64.62%), Query Frame = 2 
Query:  473 SSGQERFRSLIPSYIRDSSVAVIVYDVASRQSFLNTTKWVEEVRTERGSDVIIVLVGNKLILWTK 667
            ++GQERFR++  SY R +   ++VYDV  ++S+ N  +W++E+      +V  +LVGNK  L TK
Sbjct:   64 TAGQERFRTVTSSYYRGAHGIIVVYDVTDQESYANVKQWLQEIDRYASENVNKLLVGNKSDLTTK 128
[Close]
BLAST of FC931332 vs. ExPASy Swiss-Prot
Match: YPT53_YEAST (GTP-binding protein YPT53 OS=Saccharomyces cerevisiae GN=YPT53 PE=1 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 1.260e-18
Identity = 28/62 (45.16%), Postives = 41/62 (66.13%), Query Frame = 2 
Query:  473 SSGQERFRSLIPSYIRDSSVAVIVYDVASRQSFLNTTKWVEEVRTERGSDVIIVLVGNKLIL 658
            ++GQERF  L P Y R++  A++V+DV +  SF     WVEE+  + G D++I LVGNK+ L
Sbjct:   68 TAGQERFAPLAPMYYRNAQAALVVFDVTNEGSFYKAQNWVEELHEKVGHDIVIALVGNKMDL 129

HSP 2 Score: 50.447 bits (119), Expect = 1.260e-18
Identity = 22/59 (37.29%), Postives = 38/59 (64.41%), Query Frame = 1 
Query:  322 KLVFLGDQSVGKTSIITRFMYDKFDNTYQATIGIDFLSKTMYLEDRTVRLQLWTREIQE 498
            K+V LG+ +VGK+SI+ RF+ D F  + + TIG  FL+K +  + + ++ ++W    QE
Sbjct:   14 KVVLLGESAVGKSSIVLRFVSDDFKESKEPTIGAAFLTKRITRDGKVIKFEIWDTAGQE 72
[Close]
BLAST of FC931332 vs. ExPASy Swiss-Prot
Match: RAB1A_PIG (Ras-related protein Rab-1A OS=Sus scrofa GN=RAB1A PE=2 SV=3)

HSP 1 Score: 58.151 bits (139), Expect = 1.263e-18
Identity = 24/60 (40.00%), Postives = 41/60 (68.33%), Query Frame = 1 
Query:  319 YKLVFLGDQSVGKTSIITRFMYDKFDNTYQATIGIDFLSKTMYLEDRTVRLQLWTREIQE 498
            +KL+ +GD  VGK+ ++ RF  D +  +Y +TIG+DF  +T+ L+ +T++LQ+W    QE
Sbjct:   12 FKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQE 71

HSP 2 Score: 55.8398 bits (133), Expect = 1.263e-18
Identity = 27/65 (41.54%), Postives = 41/65 (63.08%), Query Frame = 2 
Query:  473 SSGQERFRSLIPSYIRDSSVAVIVYDVASRQSFLNTTKWVEEVRTERGSDVIIVLVGNKLILWTK 667
            ++GQERFR++  SY R +   ++VYDV  + SF N  +W++E+      +V  +LVGNK  L TK
Sbjct:   67 TAGQERFRTITSSYYRGAHGIIVVYDVTDQGSFNNVKQWLQEIDRYASENVNKLLVGNKCDLTTK 131
[Close]
BLAST of FC931332 vs. ExPASy Swiss-Prot
Match: RB22A_HUMAN (Ras-related protein Rab-22A OS=Homo sapiens GN=RAB22A PE=1 SV=2)

HSP 1 Score: 62.003 bits (149), Expect = 1.266e-18
Identity = 32/92 (34.78%), Postives = 50/92 (54.35%), Query Frame = 2 
Query:  473 SSGQERFRSLIPSYIRDSSVAVIVYDVASRQSFLNTTKWVEEVRTERGSDVIIVLVGNKLILWTKGSFIEEGEAKLVSSCYV--YETSARLA 742
            ++GQERFR+L P Y R S+ A+IVYD+   ++F     WV+E+R     ++++ + GNK  L      +E        S +    ETSA+ A
Sbjct:   61 TAGQERFRALAPMYYRGSAAAIIVYDITKEETFSTLKNWVKELRQHGPPNIVVAIAGNKCDLIDVREVMERDAKDYADSIHAIFVETSAKNA 152

HSP 2 Score: 51.9878 bits (123), Expect = 1.266e-18
Identity = 25/64 (39.06%), Postives = 37/64 (57.81%), Query Frame = 1 
Query:  307 ALAKYKLVFLGDQSVGKTSIITRFMYDKFDNTYQATIGIDFLSKTMYLEDRTVRLQLWTREIQE 498
            AL + K+  LGD  VGK+SI+ RF+ D FD     TIG  F++KT+  ++   +  +W    QE
Sbjct:    2 ALRELKVCLLGDTGVGKSSIVWRFVEDSFDPNINPTIGASFMTKTVQYQNELHKFLIWDTAGQE 65
[Close]
BLAST of FC931332 vs. ExPASy Swiss-Prot
Match: SEC4_CANGA (Ras-related protein SEC4 OS=Candida glabrata GN=SEC4 PE=3 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 1.640e-18
Identity = 26/59 (44.07%), Postives = 40/59 (67.80%), Query Frame = 1 
Query:  322 KLVFLGDQSVGKTSIITRFMYDKFDNTYQATIGIDFLSKTMYLEDRTVRLQLWTREIQE 498
            K++ +GD  VGK+ ++ RF+ DKF+ ++  TIGIDF  KT+ +  + V+LQLW    QE
Sbjct:   22 KILLIGDSGVGKSCLLVRFVEDKFNPSFITTIGIDFKIKTVDINGKKVKLQLWDTAGQE 80

HSP 2 Score: 55.8398 bits (133), Expect = 1.640e-18
Identity = 26/74 (35.14%), Postives = 44/74 (59.46%), Query Frame = 2 
Query:  473 SSGQERFRSLIPSYIRDSSVAVIVYDVASRQSFLNTTKWVEEVRTERGSDVIIVLVGNKLILWTKGSFIEEGEA 694
            ++GQERFR++  +Y R +   ++VYDV   ++F N  +W + V      +  ++LVGNK  + T+    E+GEA
Sbjct:   76 TAGQERFRTITTAYYRGAMGIILVYDVTDERTFANIKQWFKTVNEHANDEAQLLLVGNKSDMDTRVVTYEQGEA 149
[Close]
BLAST of FC931332 vs. ExPASy Swiss-Prot
Match: RB22A_MOUSE (Ras-related protein Rab-22A OS=Mus musculus GN=Rab22a PE=1 SV=2)

HSP 1 Score: 61.6178 bits (148), Expect = 1.646e-18
Identity = 32/92 (34.78%), Postives = 48/92 (52.17%), Query Frame = 2 
Query:  473 SSGQERFRSLIPSYIRDSSVAVIVYDVASRQSFLNTTKWVEEVRTERGSDVIIVLVGNKLILWTKGSFIEEGEAKLVSSCYV--YETSARLA 742
            ++GQERFR+L P Y R S+ A+IVYD+   ++F     WV E+R      +++ + GNK  L      +E        S +    ETSA+ A
Sbjct:   61 TAGQERFRALAPMYYRGSAAAIIVYDITKEETFSTLKNWVRELRQHGPPSIVVAIAGNKCDLTDVREVMERDAKDYADSIHAIFVETSAKNA 152

HSP 2 Score: 51.9878 bits (123), Expect = 1.646e-18
Identity = 25/64 (39.06%), Postives = 37/64 (57.81%), Query Frame = 1 
Query:  307 ALAKYKLVFLGDQSVGKTSIITRFMYDKFDNTYQATIGIDFLSKTMYLEDRTVRLQLWTREIQE 498
            AL + K+  LGD  VGK+SI+ RF+ D FD     TIG  F++KT+  ++   +  +W    QE
Sbjct:    2 ALRELKVCLLGDTGVGKSSIVWRFVEDSFDPNINPTIGASFMTKTVQYQNELHKFLIWDTAGQE 65
[Close]
BLAST of FC931332 vs. ExPASy Swiss-Prot
Match: SEC4_YEAST (Ras-related protein SEC4 OS=Saccharomyces cerevisiae GN=SEC4 PE=1 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 2.773e-18
Identity = 26/59 (44.07%), Postives = 40/59 (67.80%), Query Frame = 1 
Query:  322 KLVFLGDQSVGKTSIITRFMYDKFDNTYQATIGIDFLSKTMYLEDRTVRLQLWTREIQE 498
            K++ +GD  VGK+ ++ RF+ DKF+ ++  TIGIDF  KT+ +  + V+LQLW    QE
Sbjct:   22 KILLIGDSGVGKSCLLVRFVEDKFNPSFITTIGIDFKIKTVDINGKKVKLQLWDTAGQE 80

HSP 2 Score: 55.0694 bits (131), Expect = 2.773e-18
Identity = 25/74 (33.78%), Postives = 44/74 (59.46%), Query Frame = 2 
Query:  473 SSGQERFRSLIPSYIRDSSVAVIVYDVASRQSFLNTTKWVEEVRTERGSDVIIVLVGNKLILWTKGSFIEEGEA 694
            ++GQERFR++  +Y R +   ++VYDV   ++F N  +W + V      +  ++LVGNK  + T+    ++GEA
Sbjct:   76 TAGQERFRTITTAYYRGAMGIILVYDVTDERTFTNIKQWFKTVNEHANDEAQLLLVGNKSDMETRVVTADQGEA 149
[Close]
BLAST of FC931332 vs. ExPASy Swiss-Prot
Match: RAB8B_RAT (Ras-related protein Rab-8B OS=Rattus norvegicus GN=Rab8b PE=1 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 2.776e-18
Identity = 26/60 (43.33%), Postives = 41/60 (68.33%), Query Frame = 1 
Query:  319 YKLVFLGDQSVGKTSIITRFMYDKFDNTYQATIGIDFLSKTMYLEDRTVRLQLWTREIQE 498
            +KL+ +GD  VGKT ++ RF  D F+ T+ +TIGIDF  +T+ L+ + ++LQ+W    QE
Sbjct:    9 FKLLLIGDSGVGKTCLLFRFSEDAFNTTFISTIGIDFKIRTIELDGKKIKLQIWDTAGQE 68

HSP 2 Score: 53.1434 bits (126), Expect = 2.776e-18
Identity = 29/90 (32.22%), Postives = 49/90 (54.44%), Query Frame = 2 
Query:  473 SSGQERFRSLIPSYIRDSSVAVIVYDVASRQSFLNTTKWVEEVRTERGSDVIIVLVGNKLILWTKGSFIEEGEAKLVSSCYV--YETSAR 736
            ++GQERFR++  +Y R +   ++VYD+ + +SF N   W+  +     SDV  +++GNK  +  K    +E   KL     +   ETSA+
Sbjct:   64 TAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIKNWIRNIEEHASSDVERMILGNKCDMNDKRQVSKERGEKLAIDYGIKFLETSAK 153
[Close]
BLAST of FC931332 vs. ExPASy Swiss-Prot
Match: RAB8B_PONAB (Ras-related protein Rab-8B OS=Pongo abelii GN=RAB8B PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 2.776e-18
Identity = 26/60 (43.33%), Postives = 41/60 (68.33%), Query Frame = 1 
Query:  319 YKLVFLGDQSVGKTSIITRFMYDKFDNTYQATIGIDFLSKTMYLEDRTVRLQLWTREIQE 498
            +KL+ +GD  VGKT ++ RF  D F+ T+ +TIGIDF  +T+ L+ + ++LQ+W    QE
Sbjct:    9 FKLLLIGDSGVGKTCLLFRFSEDAFNTTFISTIGIDFKIRTIELDGKKIKLQIWDTAGQE 68

HSP 2 Score: 53.1434 bits (126), Expect = 2.776e-18
Identity = 29/90 (32.22%), Postives = 49/90 (54.44%), Query Frame = 2 
Query:  473 SSGQERFRSLIPSYIRDSSVAVIVYDVASRQSFLNTTKWVEEVRTERGSDVIIVLVGNKLILWTKGSFIEEGEAKLVSSCYV--YETSAR 736
            ++GQERFR++  +Y R +   ++VYD+ + +SF N   W+  +     SDV  +++GNK  +  K    +E   KL     +   ETSA+
Sbjct:   64 TAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIKNWIRNIEEHASSDVERMILGNKCDMNDKRQVSKERGEKLAIDYGIKFLETSAK 153
[Close]
BLAST of FC931332 vs. ExPASy Swiss-Prot
Match: RAB8B_MOUSE (Ras-related protein Rab-8B OS=Mus musculus GN=Rab8b PE=1 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 2.776e-18
Identity = 26/60 (43.33%), Postives = 41/60 (68.33%), Query Frame = 1 
Query:  319 YKLVFLGDQSVGKTSIITRFMYDKFDNTYQATIGIDFLSKTMYLEDRTVRLQLWTREIQE 498
            +KL+ +GD  VGKT ++ RF  D F+ T+ +TIGIDF  +T+ L+ + ++LQ+W    QE
Sbjct:    9 FKLLLIGDSGVGKTCLLFRFSEDAFNTTFISTIGIDFKIRTIELDGKKIKLQIWDTAGQE 68

HSP 2 Score: 53.1434 bits (126), Expect = 2.776e-18
Identity = 29/90 (32.22%), Postives = 49/90 (54.44%), Query Frame = 2 
Query:  473 SSGQERFRSLIPSYIRDSSVAVIVYDVASRQSFLNTTKWVEEVRTERGSDVIIVLVGNKLILWTKGSFIEEGEAKLVSSCYV--YETSAR 736
            ++GQERFR++  +Y R +   ++VYD+ + +SF N   W+  +     SDV  +++GNK  +  K    +E   KL     +   ETSA+
Sbjct:   64 TAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIKNWIRNIEEHASSDVERMILGNKCDMNDKRQVSKERGEKLAIDYGIKFLETSAK 153
The following BLAST results are available for this feature:
BLAST of FC931332 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 208
Match NameE-valueIdentityDescription
RAB1B_RAT9.717e-1940.00Ras-related protein Rab-1B OS=Rattus norvegicus GN... [more]
YPT53_YEAST1.260e-1845.16GTP-binding protein YPT53 OS=Saccharomyces cerevis... [more]
RAB1A_PIG1.263e-1840.00Ras-related protein Rab-1A OS=Sus scrofa GN=RAB1A ... [more]
RB22A_HUMAN1.266e-1834.78Ras-related protein Rab-22A OS=Homo sapiens GN=RAB... [more]
SEC4_CANGA1.640e-1844.07Ras-related protein SEC4 OS=Candida glabrata GN=SE... [more]
RB22A_MOUSE1.646e-1834.78Ras-related protein Rab-22A OS=Mus musculus GN=Rab... [more]
SEC4_YEAST2.773e-1844.07Ras-related protein SEC4 OS=Saccharomyces cerevisi... [more]
RAB8B_RAT2.776e-1843.33Ras-related protein Rab-8B OS=Rattus norvegicus GN... [more]
RAB8B_PONAB2.776e-1843.33Ras-related protein Rab-8B OS=Pongo abelii GN=RAB8... [more]
RAB8B_MOUSE2.776e-1843.33Ras-related protein Rab-8B OS=Mus musculus GN=Rab8... [more]

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Properties
Property NameValue
Genbank descriptionC34104A03EF PostHarvP1 Citrus clementina cDNA clone C34104A03, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC931332 ID=FC931332; Name=FC931332; organism=Citrus clementina; type=EST; length=743bp
ACATGGTGAGAGGCTCGCTCTGCCAGAGCCACTGAGCCAGAGCCAGCGCC
AGCGCCCGCGCACGCGTGCAGAAAATTCGTACATACACACACCTGCAGAG
AATTACTTCTATTCTATGCATAAATAAATAAAGGGGGCAGGCAGGCAGGC
AGGCAGGCATCAAAGGCAAATCCCGAAAATCCGAGGCCCCCGAGATCTCG
TCCTCCATCTCCTTCTCTCCGTTGAATACACCCGGCCGAGATCTCGTGTA
ATTGCACCAAAACTGTTGATTTTTGTTGTAGATCCGAAAAAATGGCTCCG
GTTTCAGCTCTAGCCAAGTACAAGTTGGTGTTTTTAGGGGATCAGTCCGT
GGGCAAAACTAGTATCATCACTCGCTTCATGTACGATAAATTCGACAACA
CTTATCAGGCTACCATTGGTATCGATTTCCTGTCGAAGACAATGTATCTT
GAAGACCGAACGGTTCGTTTGCAGCTCTGGACAAGAGAGATTCAGGAGTC
TCATTCCTAGCTACATCAGAGATTCCTCTGTCGCAGTCATTGTATATGAT
GTTGCAAGTAGGCAGTCTTTCCTAAACACCACCAAGTGGGTTGAAGAGGT
TCGAACTGAGCGGGGCAGTGATGTAATCATTGTCCTTGTTGGGAACAAAC
TGATCTTGTGGACAAAAGGCAGTTTCATAGAGGAGGGAGAGGCTAAGCTC
GTGAGCTCATGTTATGTTTATGAGACTAGTGCCAGGCTGGCTT
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