BQ623890

Overview
NameBQ623890
Unique NameBQ623890
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length475
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_CLAMS (Tryptophan synthase beta chain OS=Clavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / JCM 9667) GN=trpB PE=3 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 3.264e-24
Identity = 36/73 (49.32%), Postives = 48/73 (65.75%), Query Frame = 3 
Query:   96 GVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYLQV 314
            G   Y  VTDD+A+ A +  SR EGIIPA+E+AHALA   +L   L     +++N SGRGDKD++TA KY  +
Sbjct:  324 GRASYRPVTDDQAMSALRLLSRTEGIIPAIESAHALAGALELGKELGPDAIILINLSGRGDKDMETAGKYFDL 396

HSP 2 Score: 62.003 bits (149), Expect = 3.264e-24
Identity = 28/34 (82.35%), Postives = 30/34 (88.24%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            DEDGQ I+ HSISAGLDYPGVGPEHS+L D GRA
Sbjct:  293 DEDGQTIDSHSISAGLDYPGVGPEHSWLSDLGRA 326
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_LISIN (Tryptophan synthase beta chain OS=Listeria innocua GN=trpB PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 3.265e-24
Identity = 36/64 (56.25%), Postives = 46/64 (71.88%), Query Frame = 3 
Query:   96 GVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDV 287
            G  EY++VTDDEA+EAF+   R EGIIPALE++HA++Y  KL   +     +VV  SGRGDKDV
Sbjct:  324 GRAEYHSVTDDEAVEAFQLLCRTEGIIPALESSHAISYAVKLASQMRPDESMVVCLSGRGDKDV 387

HSP 2 Score: 59.6918 bits (143), Expect = 3.265e-24
Identity = 26/34 (76.47%), Postives = 30/34 (88.24%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            DE+GQI+E  SISAGLDYPG+GPEHSF +D GRA
Sbjct:  293 DENGQILEAFSISAGLDYPGIGPEHSFFRDLGRA 326
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_STRGG (Tryptophan synthase beta chain OS=Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350) GN=trpB PE=3 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 4.243e-24
Identity = 37/70 (52.86%), Postives = 46/70 (65.71%), Query Frame = 3 
Query:   96 GVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKY 305
            G  EY  VTDD A++A +  SR EGIIPA+E+AHALA    L   L     ++VN SGRGDKD+ TA +Y
Sbjct:  333 GRGEYRAVTDDAAMQALRLLSRTEGIIPAIESAHALAGALDLGKELGKDGLILVNLSGRGDKDMDTAARY 402

HSP 2 Score: 62.3882 bits (150), Expect = 4.243e-24
Identity = 26/33 (78.79%), Postives = 31/33 (93.94%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            D++GQI EP+SISAGLDYPG+GPEHS+LKD GR
Sbjct:  302 DDEGQITEPYSISAGLDYPGIGPEHSYLKDVGR 334
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_GLOVI (Tryptophan synthase beta chain OS=Gloeobacter violaceus GN=trpB PE=3 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 4.246e-24
Identity = 39/69 (56.52%), Postives = 43/69 (62.32%), Query Frame = 3 
Query:   96 GVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIK 302
            G  EYY+VTD EAL A       EGIIPALETAHA AYL  L   L     VV+N SGRGDKD+ T  +
Sbjct:  338 GRAEYYSVTDSEALAALSLVCSTEGIIPALETAHAFAYLGILASQLHSEQIVVLNCSGRGDKDMGTVAR 406

HSP 2 Score: 61.6178 bits (148), Expect = 4.246e-24
Identity = 27/34 (79.41%), Postives = 30/34 (88.24%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            DE GQ+ E HS+SAGLDYPGVGPEHS+LKD GRA
Sbjct:  307 DEQGQVQEAHSLSAGLDYPGVGPEHSYLKDIGRA 340
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_LISW6 (Tryptophan synthase beta chain OS=Listeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / SLCC5334) GN=trpB PE=3 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 4.249e-24
Identity = 36/64 (56.25%), Postives = 46/64 (71.88%), Query Frame = 3 
Query:   96 GVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDV 287
            G  EY++VTDDEA+EAF+   R EGIIPALE++HA++Y  KL   +     +VV  SGRGDKDV
Sbjct:  324 GRAEYHSVTDDEAVEAFQLLCRTEGIIPALESSHAISYAVKLASKMRPEESMVVCLSGRGDKDV 387

HSP 2 Score: 59.6918 bits (143), Expect = 4.249e-24
Identity = 26/34 (76.47%), Postives = 30/34 (88.24%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            DE+GQI+E  SISAGLDYPG+GPEHSF +D GRA
Sbjct:  293 DENGQILEAFSISAGLDYPGIGPEHSFFRDLGRA 326
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_STRCO (Tryptophan synthase beta chain OS=Streptomyces coelicolor GN=trpB PE=3 SV=2)

HSP 1 Score: 69.3218 bits (168), Expect = 5.523e-24
Identity = 38/73 (52.05%), Postives = 48/73 (65.75%), Query Frame = 3 
Query:   87 KMKGVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKY 305
            K  G  EY  VTDD A++A +  SR EGIIPA+E+AHALA   ++   L     +VVN SGRGDKD+ TA +Y
Sbjct:  330 KDSGRGEYRAVTDDAAMQALRLLSRTEGIIPAIESAHALAGALEVGRELGRDGLLVVNLSGRGDKDMDTAARY 402

HSP 2 Score: 62.003 bits (149), Expect = 5.523e-24
Identity = 25/33 (75.76%), Postives = 31/33 (93.94%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            D++GQI EP+SISAGLDYPG+GPEH++LKD GR
Sbjct:  302 DDEGQITEPYSISAGLDYPGIGPEHAYLKDSGR 334
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_ACIAC (Tryptophan synthase beta chain OS=Acidovorax avenae subsp. citrulli (strain AAC00-1) GN=trpB PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 5.523e-24
Identity = 36/66 (54.55%), Postives = 45/66 (68.18%), Query Frame = 3 
Query:   96 GVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQT 293
            G  EY  +TD EAL+AF    R EGIIPALE++HA+AY  +L  TL     ++VN SGRGDKD+ T
Sbjct:  331 GRAEYVGITDREALDAFHYLCRTEGIIPALESSHAVAYAMQLARTLRTDQSILVNLSGRGDKDIGT 396

HSP 2 Score: 58.9214 bits (141), Expect = 5.523e-24
Identity = 26/34 (76.47%), Postives = 31/34 (91.18%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            DE+GQI E HSISAGLDYPGVGPEH++L++ GRA
Sbjct:  300 DENGQITETHSISAGLDYPGVGPEHAWLQEIGRA 333
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_PSEPW (Tryptophan synthase beta chain OS=Pseudomonas putida (strain W619) GN=trpB PE=3 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 5.528e-24
Identity = 38/68 (55.88%), Postives = 49/68 (72.06%), Query Frame = 3 
Query:  105 EYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYL 308
            EY ++TDDEAL+AF  + RLEGIIPALET+HALA   K  P L     +V+  SGRGDKD+QT + ++
Sbjct:  331 EYVSITDDEALDAFHATCRLEGIIPALETSHALAEAIKRAPNLPKDHLMVICLSGRGDKDMQTVMNHM 398

HSP 2 Score: 53.9138 bits (128), Expect = 5.528e-24
Identity = 22/33 (66.67%), Postives = 28/33 (84.85%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            D+DGQI + HSISAGLDYPG+GPEH++L +  R
Sbjct:  297 DDDGQITDAHSISAGLDYPGIGPEHAYLHEVKR 329
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_NOVAD (Tryptophan synthase beta chain OS=Novosphingobium aromaticivorans (strain DSM 12444) GN=trpB PE=3 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 5.528e-24
Identity = 34/70 (48.57%), Postives = 49/70 (70.00%), Query Frame = 3 
Query:  105 EYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYLQV 314
            +Y +VTD EAL+AF+   R EGIIPALE AHA+A ++K+ PT+     ++ N  GRGDKD+ +  ++L V
Sbjct:  333 DYTSVTDTEALDAFQLLCRTEGIIPALEPAHAIAAVKKVAPTMGKDEIILANLCGRGDKDIFSVAEHLGV 402

HSP 2 Score: 60.077 bits (144), Expect = 5.528e-24
Identity = 26/33 (78.79%), Postives = 30/33 (90.91%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            DEDGQI E HSISAGLDYPG+GPEH++LK+ GR
Sbjct:  299 DEDGQITEGHSISAGLDYPGIGPEHAWLKEIGR 331
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB1_ARCFU (Tryptophan synthase beta chain 1 OS=Archaeoglobus fulgidus GN=trpB1 PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 5.530e-24
Identity = 38/67 (56.72%), Postives = 46/67 (68.66%), Query Frame = 3 
Query:   87 KMKGVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDV 287
            K  G  EY  V D+EAL AFK  S+LEGIIPALE+AHA+AY  K+   +     +VVN SGRGDKD+
Sbjct:  323 KETGRCEYVTVNDEEALRAFKTLSKLEGIIPALESAHAIAYAMKMAEEMQRDDVLVVNLSGRGDKDM 389

HSP 2 Score: 57.3806 bits (137), Expect = 5.530e-24
Identity = 22/33 (66.67%), Postives = 30/33 (90.91%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            DE+G +++ HS+SAGLDYPGVGPEH++LK+ GR
Sbjct:  295 DEEGMMLDTHSVSAGLDYPGVGPEHAYLKETGR 327
The following BLAST results are available for this feature:
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 410
Match NameE-valueIdentityDescription
TRPB_CLAMS3.264e-2449.32Tryptophan synthase beta chain OS=Clavibacter mich... [more]
TRPB_LISIN3.265e-2456.25Tryptophan synthase beta chain OS=Listeria innocua... [more]
TRPB_STRGG4.243e-2452.86Tryptophan synthase beta chain OS=Streptomyces gri... [more]
TRPB_GLOVI4.246e-2456.52Tryptophan synthase beta chain OS=Gloeobacter viol... [more]
TRPB_LISW64.249e-2456.25Tryptophan synthase beta chain OS=Listeria welshim... [more]
TRPB_STRCO5.523e-2452.05Tryptophan synthase beta chain OS=Streptomyces coe... [more]
TRPB_ACIAC5.523e-2454.55Tryptophan synthase beta chain OS=Acidovorax avena... [more]
TRPB_PSEPW5.528e-2455.88Tryptophan synthase beta chain OS=Pseudomonas puti... [more]
TRPB_NOVAD5.528e-2448.57Tryptophan synthase beta chain OS=Novosphingobium ... [more]
TRPB1_ARCFU5.530e-2456.72Tryptophan synthase beta chain 1 OS=Archaeoglobus ... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00970 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00970 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623890 ID=BQ623890; Name=BQ623890; organism=Citrus sinensis; type=EST; length=475bp
GGATGAAGATGGGCAAATAATTGAGCCACACTCCATTAGTGCAGGCTTAG
ATTACCCTGGAGTTGGACCTGAGCATAGTTTTCTGAAAGATGAAGGGCGT
GCGTGAGTACTATAATGTCACAGATGATGAAGCATTGGAAGCTTTCAAGA
GGTCATCACGGCTAGAGGGTATAATCCCAGCTTTGGAGACTGCTCATGCA
TTGGCTTATTTGGAGAAGCTATGCCCAACTCTTGCTGATGGTACTAAGGT
TGTGGTTAACTTCAGTGGCAGAGGTGACAAGGATGTCCAGACAGCGATCA
AATATTTGCAAGTTTGATGGTAATGCTTGTAACCCACAATATATGATTCA
TTTACTGAAAACAAATAAATGGGTTGCTTGAGCTTCTGTTTTTGAATTTT
GTATCATTAGTTATTTCAGAGATGTGAGAACCATCTTATGTAATTTGAGT
TAAAACTTTATATATTACATCTATT
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