BQ623890

Overview
NameBQ623890
Unique NameBQ623890
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length475
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_PSEPU (Tryptophan synthase beta chain OS=Pseudomonas putida GN=trpB PE=3 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 7.193e-24
Identity = 38/68 (55.88%), Postives = 49/68 (72.06%), Query Frame = 3 
Query:  105 EYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYL 308
            EY ++TDDEAL+AF  + RLEGIIPALE++HALA   K  P L     +VV  SGRGDKD+QT + ++
Sbjct:  331 EYVSITDDEALDAFHATCRLEGIIPALESSHALAEAIKRAPKLPKDHLMVVCLSGRGDKDMQTVMNHM 398

HSP 2 Score: 55.0694 bits (131), Expect = 7.193e-24
Identity = 23/33 (69.70%), Postives = 28/33 (84.85%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            DEDGQI + HSISAGLDYPG+GPEH++L +  R
Sbjct:  297 DEDGQITDAHSISAGLDYPGIGPEHAYLHEVKR 329
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_AGRRK (Tryptophan synthase beta chain OS=Agrobacterium radiobacter (strain K84 / ATCC BAA-868) GN=trpB PE=3 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 7.193e-24
Identity = 37/71 (52.11%), Postives = 47/71 (66.20%), Query Frame = 3 
Query:   96 GVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYL 308
            G  EY  + D EALEAF+  +RLEGIIPALE +HALA + K  P +     +++N SGRGDKD+ T  K L
Sbjct:  332 GRAEYVPIMDHEALEAFQTLTRLEGIIPALEPSHALAEVIKRAPKMGKDEIILMNLSGRGDKDIFTVAKIL 402

HSP 2 Score: 58.9214 bits (141), Expect = 7.193e-24
Identity = 27/34 (79.41%), Postives = 29/34 (85.29%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            D DGQI E HSISAGLDYPG+GPEHS+L D GRA
Sbjct:  301 DGDGQIKEGHSISAGLDYPGIGPEHSWLNDIGRA 334
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_AGRT5 (Tryptophan synthase beta chain OS=Agrobacterium tumefaciens (strain C58 / ATCC 33970) GN=trpB PE=3 SV=2)

HSP 1 Score: 72.7886 bits (177), Expect = 1.218e-23
Identity = 36/68 (52.94%), Postives = 47/68 (69.12%), Query Frame = 3 
Query:  105 EYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYL 308
            EY  + D EAL+AF+  +RLEGIIPALE +HALA + K  P +     +++N SGRGDKDV T  K+L
Sbjct:  335 EYVPIMDHEALDAFQMLTRLEGIIPALEPSHALAEVIKRAPKMGKDEIILMNLSGRGDKDVHTVSKFL 402

HSP 2 Score: 57.3806 bits (137), Expect = 1.218e-23
Identity = 26/33 (78.79%), Postives = 28/33 (84.85%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            D DGQI E HSISAGLDYPG+GPEHS+L D GR
Sbjct:  301 DGDGQIKEGHSISAGLDYPGIGPEHSWLNDIGR 333
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_PSEU2 (Tryptophan synthase beta chain OS=Pseudomonas syringae pv. syringae (strain B728a) GN=trpB PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.585e-23
Identity = 39/68 (57.35%), Postives = 48/68 (70.59%), Query Frame = 3 
Query:  105 EYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYL 308
            EY ++TDDEAL+AF +   LEGIIPALETAHALA   K    L D   +VV  SGRGDKD+QT + ++
Sbjct:  331 EYVSITDDEALDAFHQCCLLEGIIPALETAHALAEAMKRATNLRDDHLMVVCLSGRGDKDMQTVMNHM 398

HSP 2 Score: 53.5286 bits (127), Expect = 1.585e-23
Identity = 23/33 (69.70%), Postives = 27/33 (81.82%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            D DGQI + HSISAGLDYPG+GPEH+FL +  R
Sbjct:  297 DGDGQITDAHSISAGLDYPGIGPEHAFLHEVKR 329
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_PSE14 (Tryptophan synthase beta chain OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=trpB PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.585e-23
Identity = 39/68 (57.35%), Postives = 48/68 (70.59%), Query Frame = 3 
Query:  105 EYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYL 308
            EY ++TDDEAL+AF +   LEGIIPALETAHALA   K    L D   +VV  SGRGDKD+QT + ++
Sbjct:  331 EYVSITDDEALDAFHQCCLLEGIIPALETAHALAEAMKRATNLRDDHLMVVCLSGRGDKDMQTVMNHM 398

HSP 2 Score: 53.5286 bits (127), Expect = 1.585e-23
Identity = 23/33 (69.70%), Postives = 27/33 (81.82%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            D DGQI + HSISAGLDYPG+GPEH+FL +  R
Sbjct:  297 DGDGQITDAHSISAGLDYPGIGPEHAFLHEVKR 329
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_PSEPK (Tryptophan synthase beta chain OS=Pseudomonas putida (strain KT2440) GN=trpB PE=3 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.585e-23
Identity = 38/68 (55.88%), Postives = 49/68 (72.06%), Query Frame = 3 
Query:  105 EYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYL 308
            EY ++TDDEAL+AF  + RLEGIIPALE++HALA   K  P L     +VV  SGRGDKD+QT + ++
Sbjct:  331 EYVSITDDEALDAFHATCRLEGIIPALESSHALAEAIKRAPKLPKDHLMVVCLSGRGDKDMQTVMNHM 398

HSP 2 Score: 53.9138 bits (128), Expect = 1.585e-23
Identity = 22/33 (66.67%), Postives = 28/33 (84.85%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            D+DGQI + HSISAGLDYPG+GPEH++L +  R
Sbjct:  297 DQDGQITDAHSISAGLDYPGIGPEHAYLHEVKR 329
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_PSEP1 (Tryptophan synthase beta chain OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=trpB PE=3 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.585e-23
Identity = 38/68 (55.88%), Postives = 49/68 (72.06%), Query Frame = 3 
Query:  105 EYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYL 308
            EY ++TDDEAL+AF  + RLEGIIPALE++HALA   K  P L     +VV  SGRGDKD+QT + ++
Sbjct:  331 EYVSITDDEALDAFHATCRLEGIIPALESSHALAEAIKRAPKLPKDHLMVVCLSGRGDKDMQTVMNHM 398

HSP 2 Score: 53.9138 bits (128), Expect = 1.585e-23
Identity = 22/33 (66.67%), Postives = 28/33 (84.85%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            D+DGQI + HSISAGLDYPG+GPEH++L +  R
Sbjct:  297 DQDGQITDAHSISAGLDYPGIGPEHAYLHEVKR 329
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_RHIE6 (Tryptophan synthase beta chain OS=Rhizobium etli (strain CIAT 652) GN=trpB PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.585e-23
Identity = 36/68 (52.94%), Postives = 48/68 (70.59%), Query Frame = 3 
Query:  105 EYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYL 308
            +Y  + D EALEAF+  +RLEGIIPALE AHA+A + K  PT++    +++N SGRGDKD+ T  K L
Sbjct:  335 DYVPIMDHEALEAFQTLTRLEGIIPALEAAHAIAEVIKRAPTMSKDEIILMNLSGRGDKDIFTVGKIL 402

HSP 2 Score: 57.3806 bits (137), Expect = 1.585e-23
Identity = 26/33 (78.79%), Postives = 28/33 (84.85%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            D DGQI E HSISAGLDYPG+GPEHS+L D GR
Sbjct:  301 DGDGQIKEGHSISAGLDYPGIGPEHSWLSDIGR 333
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_HELPJ (Tryptophan synthase beta chain OS=Helicobacter pylori J99 GN=trpB PE=3 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 1.586e-23
Identity = 36/68 (52.94%), Postives = 45/68 (66.18%), Query Frame = 3 
Query:  108 YYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYLQ 311
            Y + +D EALEAF+   + EGIIPALE++HALAY  KL    A    +VVN SGRGDKD+ T    L+
Sbjct:  322 YESASDIEALEAFRLLCQKEGIIPALESSHALAYALKLAQKCAQEKIIVVNLSGRGDKDLSTVYNALK 389

HSP 2 Score: 61.6178 bits (148), Expect = 1.586e-23
Identity = 27/34 (79.41%), Postives = 31/34 (91.18%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            D++GQI E HSISAGLDYPGVGPEHS+LK+ GRA
Sbjct:  287 DDEGQIAESHSISAGLDYPGVGPEHSYLKEIGRA 320
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB2_COREF (Tryptophan synthase beta chain 2 OS=Corynebacterium efficiens GN=trpB2 PE=3 SV=2)

HSP 1 Score: 71.2478 bits (173), Expect = 2.059e-23
Identity = 36/67 (53.73%), Postives = 45/67 (67.16%), Query Frame = 3 
Query:   87 KMKGVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDV 287
            K  G  EY   TD EAL+AF+  SR EGIIPALE++HALA+  K+     +   ++VN SGRGDKDV
Sbjct:  334 KDTGRAEYVGATDAEALQAFRMLSRYEGIIPALESSHALAHALKMAAEATEPINILVNLSGRGDKDV 400

HSP 2 Score: 58.151 bits (139), Expect = 2.059e-23
Identity = 25/34 (73.53%), Postives = 28/34 (82.35%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            DEDG +   HS+SAGLDYPGVGPEH+ LKD GRA
Sbjct:  306 DEDGNLRNSHSVSAGLDYPGVGPEHAHLKDTGRA 339
The following BLAST results are available for this feature:
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 410
Match NameE-valueIdentityDescription
TRPB_PSEPU7.193e-2455.88Tryptophan synthase beta chain OS=Pseudomonas puti... [more]
TRPB_AGRRK7.193e-2452.11Tryptophan synthase beta chain OS=Agrobacterium ra... [more]
TRPB_AGRT51.218e-2352.94Tryptophan synthase beta chain OS=Agrobacterium tu... [more]
TRPB_PSEU21.585e-2357.35Tryptophan synthase beta chain OS=Pseudomonas syri... [more]
TRPB_PSE141.585e-2357.35Tryptophan synthase beta chain OS=Pseudomonas syri... [more]
TRPB_PSEPK1.585e-2355.88Tryptophan synthase beta chain OS=Pseudomonas puti... [more]
TRPB_PSEP11.585e-2355.88Tryptophan synthase beta chain OS=Pseudomonas puti... [more]
TRPB_RHIE61.585e-2352.94Tryptophan synthase beta chain OS=Rhizobium etli (... [more]
TRPB_HELPJ1.586e-2352.94Tryptophan synthase beta chain OS=Helicobacter pyl... [more]
TRPB2_COREF2.059e-2353.73Tryptophan synthase beta chain 2 OS=Corynebacteriu... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00970 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00970 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623890 ID=BQ623890; Name=BQ623890; organism=Citrus sinensis; type=EST; length=475bp
GGATGAAGATGGGCAAATAATTGAGCCACACTCCATTAGTGCAGGCTTAG
ATTACCCTGGAGTTGGACCTGAGCATAGTTTTCTGAAAGATGAAGGGCGT
GCGTGAGTACTATAATGTCACAGATGATGAAGCATTGGAAGCTTTCAAGA
GGTCATCACGGCTAGAGGGTATAATCCCAGCTTTGGAGACTGCTCATGCA
TTGGCTTATTTGGAGAAGCTATGCCCAACTCTTGCTGATGGTACTAAGGT
TGTGGTTAACTTCAGTGGCAGAGGTGACAAGGATGTCCAGACAGCGATCA
AATATTTGCAAGTTTGATGGTAATGCTTGTAACCCACAATATATGATTCA
TTTACTGAAAACAAATAAATGGGTTGCTTGAGCTTCTGTTTTTGAATTTT
GTATCATTAGTTATTTCAGAGATGTGAGAACCATCTTATGTAATTTGAGT
TAAAACTTTATATATTACATCTATT
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