BQ623890

Overview
NameBQ623890
Unique NameBQ623890
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length475
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_JANSC (Tryptophan synthase beta chain OS=Jannaschia sp. (strain CCS1) GN=trpB PE=3 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 2.062e-23
Identity = 34/71 (47.89%), Postives = 48/71 (67.61%), Query Frame = 3 
Query:   96 GVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYL 308
            G  +Y ++TD EAL+AF+ S   EGIIPALE +HALA++ K+ P +     +V+N  GRGDKD+ T  + L
Sbjct:  332 GRAQYVSITDREALDAFQLSCETEGIIPALEPSHALAHVCKIAPDMPRDHLLVMNMCGRGDKDIFTVARAL 402

HSP 2 Score: 60.4622 bits (145), Expect = 2.062e-23
Identity = 26/34 (76.47%), Postives = 31/34 (91.18%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            DEDGQI+E HSISAGLDYPG+GPEH++L + GRA
Sbjct:  301 DEDGQILEGHSISAGLDYPGIGPEHAWLHEIGRA 334
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_PSEE4 (Tryptophan synthase beta chain OS=Pseudomonas entomophila (strain L48) GN=trpB PE=3 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 2.062e-23
Identity = 37/68 (54.41%), Postives = 49/68 (72.06%), Query Frame = 3 
Query:  105 EYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYL 308
            EY ++TDDEAL+AF  + RLEGIIPALE++HALA   K  P L     +V+  SGRGDKD+QT + ++
Sbjct:  331 EYVSITDDEALDAFHATCRLEGIIPALESSHALAEAIKRAPKLPKDHLMVICLSGRGDKDMQTVMNHM 398

HSP 2 Score: 53.9138 bits (128), Expect = 2.062e-23
Identity = 22/33 (66.67%), Postives = 28/33 (84.85%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            D+DGQI + HSISAGLDYPG+GPEH++L +  R
Sbjct:  297 DDDGQITDAHSISAGLDYPGIGPEHAYLHEVKR 329
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_RHIL3 (Tryptophan synthase beta chain OS=Rhizobium leguminosarum bv. viciae (strain 3841) GN=trpB PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 2.062e-23
Identity = 35/68 (51.47%), Postives = 47/68 (69.12%), Query Frame = 3 
Query:  105 EYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYL 308
            +Y  + D EALEAF+  +RLEGIIPALE +HA+A + K  PT+     +++N SGRGDKD+ T  K L
Sbjct:  335 DYVPIMDHEALEAFQTLTRLEGIIPALEPSHAIAEVIKRAPTMGKDEIILMNLSGRGDKDIFTVGKIL 402

HSP 2 Score: 58.9214 bits (141), Expect = 2.062e-23
Identity = 26/33 (78.79%), Postives = 28/33 (84.85%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            D DGQI E HSISAGLDYPG+GPEHS+L D GR
Sbjct:  301 DSDGQIKEGHSISAGLDYPGIGPEHSWLNDTGR 333
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_LISMO (Tryptophan synthase beta chain OS=Listeria monocytogenes GN=trpB PE=3 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 2.063e-23
Identity = 35/64 (54.69%), Postives = 45/64 (70.31%), Query Frame = 3 
Query:   96 GVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDV 287
            G   Y++VTDDEA+EAF+   R EGIIPALE++HA++Y  KL   +     +VV  SGRGDKDV
Sbjct:  324 GRAAYHSVTDDEAVEAFQLLCRTEGIIPALESSHAISYAVKLASKMRPEESMVVCLSGRGDKDV 387

HSP 2 Score: 59.6918 bits (143), Expect = 2.063e-23
Identity = 26/34 (76.47%), Postives = 30/34 (88.24%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            DE+GQI+E  SISAGLDYPG+GPEHSF +D GRA
Sbjct:  293 DENGQILEAFSISAGLDYPGIGPEHSFFRDLGRA 326
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_LISMH (Tryptophan synthase beta chain OS=Listeria monocytogenes serotype 4a (strain HCC23) GN=trpB PE=3 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 2.063e-23
Identity = 35/64 (54.69%), Postives = 45/64 (70.31%), Query Frame = 3 
Query:   96 GVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDV 287
            G   Y++VTDDEA+EAF+   R EGIIPALE++HA++Y  KL   +     +VV  SGRGDKDV
Sbjct:  324 GRAAYHSVTDDEAVEAFQLLCRTEGIIPALESSHAISYAVKLASQMRPEESMVVCLSGRGDKDV 387

HSP 2 Score: 59.6918 bits (143), Expect = 2.063e-23
Identity = 26/34 (76.47%), Postives = 30/34 (88.24%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            DE+GQI+E  SISAGLDYPG+GPEHSF +D GRA
Sbjct:  293 DENGQILEAFSISAGLDYPGIGPEHSFFRDLGRA 326
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_LISMF (Tryptophan synthase beta chain OS=Listeria monocytogenes serotype 4b (strain F2365) GN=trpB PE=3 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 2.063e-23
Identity = 35/64 (54.69%), Postives = 45/64 (70.31%), Query Frame = 3 
Query:   96 GVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDV 287
            G   Y++VTDDEA+EAF+   R EGIIPALE++HA++Y  KL   +     +VV  SGRGDKDV
Sbjct:  324 GRAAYHSVTDDEAVEAFQLLCRTEGIIPALESSHAISYAVKLASQMRPEESMVVCLSGRGDKDV 387

HSP 2 Score: 59.6918 bits (143), Expect = 2.063e-23
Identity = 26/34 (76.47%), Postives = 30/34 (88.24%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            DE+GQI+E  SISAGLDYPG+GPEHSF +D GRA
Sbjct:  293 DENGQILEAFSISAGLDYPGIGPEHSFFRDLGRA 326
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_LISMC (Tryptophan synthase beta chain OS=Listeria monocytogenes serotype 4b (strain Clip81459) GN=trpB PE=3 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 2.063e-23
Identity = 35/64 (54.69%), Postives = 45/64 (70.31%), Query Frame = 3 
Query:   96 GVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDV 287
            G   Y++VTDDEA+EAF+   R EGIIPALE++HA++Y  KL   +     +VV  SGRGDKDV
Sbjct:  324 GRAAYHSVTDDEAVEAFQLLCRTEGIIPALESSHAISYAVKLASQMRPEESMVVCLSGRGDKDV 387

HSP 2 Score: 59.6918 bits (143), Expect = 2.063e-23
Identity = 26/34 (76.47%), Postives = 30/34 (88.24%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            DE+GQI+E  SISAGLDYPG+GPEHSF +D GRA
Sbjct:  293 DENGQILEAFSISAGLDYPGIGPEHSFFRDLGRA 326
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_ROSDO (Tryptophan synthase beta chain OS=Roseobacter denitrificans (strain ATCC 33942 / OCh 114) GN=trpB PE=3 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 2.683e-23
Identity = 36/71 (50.70%), Postives = 47/71 (66.20%), Query Frame = 3 
Query:   96 GVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYL 308
            G  +Y ++TD EALEAF+    LEGIIPALE +HALA++ K+ P L     + +N  GRGDKD+ T  K L
Sbjct:  332 GRAQYVSITDVEALEAFQLCCELEGIIPALEPSHALAHVMKIAPDLPSDHIICMNMCGRGDKDIFTVAKAL 402

HSP 2 Score: 57.3806 bits (137), Expect = 2.683e-23
Identity = 25/34 (73.53%), Postives = 30/34 (88.24%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            D+DGQI+E  SISAGLDYPG+GPEH++L D GRA
Sbjct:  301 DDDGQILEGFSISAGLDYPGIGPEHAWLHDIGRA 334
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_RHOPT (Tryptophan synthase beta chain OS=Rhodopseudomonas palustris (strain TIE-1) GN=trpB PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 2.684e-23
Identity = 36/67 (53.73%), Postives = 46/67 (68.66%), Query Frame = 3 
Query:  108 YYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYL 308
            + + TD EAL+AFK   RLEGIIPALE +HALA +  L P L     +VVN SGRGDKD+ +  ++L
Sbjct:  334 FLSATDTEALDAFKLCCRLEGIIPALEPSHALAKVADLAPKLPKDHLMVVNMSGRGDKDLASVAEHL 400

HSP 2 Score: 58.5362 bits (140), Expect = 2.684e-23
Identity = 27/33 (81.82%), Postives = 28/33 (84.85%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            D DGQI E HSISAGLDYPGVGPEHS+L D GR
Sbjct:  299 DADGQIEEAHSISAGLDYPGVGPEHSWLHDVGR 331
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_RHOPA (Tryptophan synthase beta chain OS=Rhodopseudomonas palustris GN=trpB PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 2.684e-23
Identity = 36/67 (53.73%), Postives = 46/67 (68.66%), Query Frame = 3 
Query:  108 YYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYL 308
            + + TD EAL+AFK   RLEGIIPALE +HALA +  L P L     +VVN SGRGDKD+ +  ++L
Sbjct:  334 FLSATDTEALDAFKLCCRLEGIIPALEPSHALAKVADLAPKLPKDHLMVVNMSGRGDKDLASVAEHL 400

HSP 2 Score: 58.5362 bits (140), Expect = 2.684e-23
Identity = 27/33 (81.82%), Postives = 28/33 (84.85%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            D DGQI E HSISAGLDYPGVGPEHS+L D GR
Sbjct:  299 DADGQIEEAHSISAGLDYPGVGPEHSWLHDVGR 331
The following BLAST results are available for this feature:
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 410
Match NameE-valueIdentityDescription
TRPB_JANSC2.062e-2347.89Tryptophan synthase beta chain OS=Jannaschia sp. (... [more]
TRPB_PSEE42.062e-2354.41Tryptophan synthase beta chain OS=Pseudomonas ento... [more]
TRPB_RHIL32.062e-2351.47Tryptophan synthase beta chain OS=Rhizobium legumi... [more]
TRPB_LISMO2.063e-2354.69Tryptophan synthase beta chain OS=Listeria monocyt... [more]
TRPB_LISMH2.063e-2354.69Tryptophan synthase beta chain OS=Listeria monocyt... [more]
TRPB_LISMF2.063e-2354.69Tryptophan synthase beta chain OS=Listeria monocyt... [more]
TRPB_LISMC2.063e-2354.69Tryptophan synthase beta chain OS=Listeria monocyt... [more]
TRPB_ROSDO2.683e-2350.70Tryptophan synthase beta chain OS=Roseobacter deni... [more]
TRPB_RHOPT2.684e-2353.73Tryptophan synthase beta chain OS=Rhodopseudomonas... [more]
TRPB_RHOPA2.684e-2353.73Tryptophan synthase beta chain OS=Rhodopseudomonas... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00970 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00970 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623890 ID=BQ623890; Name=BQ623890; organism=Citrus sinensis; type=EST; length=475bp
GGATGAAGATGGGCAAATAATTGAGCCACACTCCATTAGTGCAGGCTTAG
ATTACCCTGGAGTTGGACCTGAGCATAGTTTTCTGAAAGATGAAGGGCGT
GCGTGAGTACTATAATGTCACAGATGATGAAGCATTGGAAGCTTTCAAGA
GGTCATCACGGCTAGAGGGTATAATCCCAGCTTTGGAGACTGCTCATGCA
TTGGCTTATTTGGAGAAGCTATGCCCAACTCTTGCTGATGGTACTAAGGT
TGTGGTTAACTTCAGTGGCAGAGGTGACAAGGATGTCCAGACAGCGATCA
AATATTTGCAAGTTTGATGGTAATGCTTGTAACCCACAATATATGATTCA
TTTACTGAAAACAAATAAATGGGTTGCTTGAGCTTCTGTTTTTGAATTTT
GTATCATTAGTTATTTCAGAGATGTGAGAACCATCTTATGTAATTTGAGT
TAAAACTTTATATATTACATCTATT
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