BQ623890

Overview
NameBQ623890
Unique NameBQ623890
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length475
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_STAES (Tryptophan synthase beta chain OS=Staphylococcus epidermidis (strain ATCC 12228) GN=trpB PE=3 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 2.684e-23
Identity = 35/66 (53.03%), Postives = 50/66 (75.76%), Query Frame = 3 
Query:  108 YYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKY 305
            Y + TD+EA+EA    S++EGIIPA+E+AHAL+Y+EKL P + +   +VV  SGRGDKD++T  +Y
Sbjct:  328 YVSATDNEAMEALITFSKVEGIIPAIESAHALSYVEKLAPNMDEKEIIVVTISGRGDKDMETIKQY 393

HSP 2 Score: 53.1434 bits (126), Expect = 2.684e-23
Identity = 23/33 (69.70%), Postives = 27/33 (81.82%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            ++DGQI   HSISAGLDYPG+GPEHS+  D GR
Sbjct:  293 NDDGQIELAHSISAGLDYPGIGPEHSYYNDIGR 325
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_STAEQ (Tryptophan synthase beta chain OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN=trpB PE=3 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 2.684e-23
Identity = 35/66 (53.03%), Postives = 50/66 (75.76%), Query Frame = 3 
Query:  108 YYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKY 305
            Y + TD+EA+EA    S++EGIIPA+E+AHAL+Y+EKL P + +   +VV  SGRGDKD++T  +Y
Sbjct:  328 YVSATDNEAMEALITFSKVEGIIPAIESAHALSYVEKLAPNMDEKEIIVVTISGRGDKDMETIKQY 393

HSP 2 Score: 53.1434 bits (126), Expect = 2.684e-23
Identity = 23/33 (69.70%), Postives = 27/33 (81.82%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            ++DGQI   HSISAGLDYPG+GPEHS+  D GR
Sbjct:  293 NDDGQIELAHSISAGLDYPGIGPEHSYYNDIGR 325
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_PSEPG (Tryptophan synthase beta chain OS=Pseudomonas putida (strain GB-1) GN=trpB PE=3 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 3.492e-23
Identity = 38/68 (55.88%), Postives = 49/68 (72.06%), Query Frame = 3 
Query:  105 EYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYL 308
            EY ++TDDEAL+AF  + RLEGIIPALE++HALA   K  P L     +VV  SGRGDKD+QT + ++
Sbjct:  331 EYVSITDDEALDAFHATCRLEGIIPALESSHALAEAIKRAPKLPKDHLMVVCLSGRGDKDMQTVMNHM 398

HSP 2 Score: 52.7582 bits (125), Expect = 3.492e-23
Identity = 22/33 (66.67%), Postives = 27/33 (81.82%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            D DGQI + HSISAGLDYPG+GPEH++L +  R
Sbjct:  297 DADGQITDAHSISAGLDYPGIGPEHAYLHEVKR 329
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_LACCB (Tryptophan synthase beta chain OS=Lactobacillus casei (strain BL23) GN=trpB PE=3 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 4.544e-23
Identity = 33/71 (46.48%), Postives = 49/71 (69.01%), Query Frame = 3 
Query:   93 KGVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKY 305
            +G  +Y  +TDDEA+EAF   ++ EGI+ A+E+ HA+AY+EK+ P +A    ++   SGRGDKDV +  KY
Sbjct:  329 EGRAQYVGITDDEAVEAFTYIAKQEGIVAAIESCHAIAYVEKIAPQMAKDQIIICTLSGRGDKDVASIAKY 399

HSP 2 Score: 53.5286 bits (127), Expect = 4.544e-23
Identity = 25/34 (73.53%), Postives = 28/34 (82.35%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            +EDGQI   +SISAGLDYPGVGPEH+ L  EGRA
Sbjct:  299 NEDGQIDPVYSISAGLDYPGVGPEHAALAQEGRA 332
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_LACCA (Tryptophan synthase beta chain OS=Lactobacillus casei GN=trpB PE=3 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 4.544e-23
Identity = 33/71 (46.48%), Postives = 49/71 (69.01%), Query Frame = 3 
Query:   93 KGVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKY 305
            +G  +Y  +TDDEA+EAF   ++ EGI+ A+E+ HA+AY+EK+ P +A    ++   SGRGDKDV +  KY
Sbjct:  329 EGRAQYVGITDDEAVEAFTYIAKQEGIVAAIESCHAIAYVEKIAPQMAKDQIIICTLSGRGDKDVASIAKY 399

HSP 2 Score: 53.5286 bits (127), Expect = 4.544e-23
Identity = 25/34 (73.53%), Postives = 28/34 (82.35%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            +EDGQI   +SISAGLDYPGVGPEH+ L  EGRA
Sbjct:  299 NEDGQIDPVYSISAGLDYPGVGPEHAALAQEGRA 332
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_LACC3 (Tryptophan synthase beta chain OS=Lactobacillus casei (strain ATCC 334) GN=trpB PE=3 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 4.544e-23
Identity = 33/71 (46.48%), Postives = 49/71 (69.01%), Query Frame = 3 
Query:   93 KGVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKY 305
            +G  +Y  +TDDEA+EAF   ++ EGI+ A+E+ HA+AY+EK+ P +A    ++   SGRGDKDV +  KY
Sbjct:  329 EGRAQYVGITDDEAVEAFTYIAKQEGIVAAIESCHAIAYVEKIAPQMAKDQIIICTLSGRGDKDVASIAKY 399

HSP 2 Score: 53.5286 bits (127), Expect = 4.544e-23
Identity = 25/34 (73.53%), Postives = 28/34 (82.35%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            +EDGQI   +SISAGLDYPGVGPEH+ L  EGRA
Sbjct:  299 NEDGQIDPVYSISAGLDYPGVGPEHAALAQEGRA 332
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_PARL1 (Tryptophan synthase beta chain OS=Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966) GN=trpB PE=3 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 4.544e-23
Identity = 38/73 (52.05%), Postives = 47/73 (64.38%), Query Frame = 3 
Query:   96 GVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYLQV 314
            G  EY + TD EAL AF+  S+LEGIIPALE AHALA++ K+   L     +V+N  GRGDKDV     +L V
Sbjct:  331 GRAEYVSATDREALAAFQLCSKLEGIIPALEPAHALAFVTKIASALPKDHLMVMNMCGRGDKDVFAVADHLGV 403

HSP 2 Score: 56.9954 bits (136), Expect = 4.544e-23
Identity = 24/34 (70.59%), Postives = 30/34 (88.24%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            D+DGQI + HSISAGLDYPG+GPEH++L + GRA
Sbjct:  300 DDDGQIKDGHSISAGLDYPGIGPEHAWLHETGRA 333
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_RHOPS (Tryptophan synthase beta chain OS=Rhodopseudomonas palustris (strain BisB5) GN=trpB PE=3 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 4.544e-23
Identity = 36/67 (53.73%), Postives = 46/67 (68.66%), Query Frame = 3 
Query:  108 YYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYL 308
            + + TD EAL+AFK   RLEGIIPALE AHALA +  L P L     +V+N SGRGDKD+ +  ++L
Sbjct:  334 FLSATDAEALDAFKLCCRLEGIIPALEPAHALAKVADLAPILPKDHLMVLNMSGRGDKDLASVAEHL 400

HSP 2 Score: 58.151 bits (139), Expect = 4.544e-23
Identity = 25/33 (75.76%), Postives = 29/33 (87.88%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            D+DGQI E HSISAGLDYPG+GPEH++L D GR
Sbjct:  299 DDDGQIQEAHSISAGLDYPGIGPEHAWLHDVGR 331
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_STRM5 (Tryptophan synthase beta chain OS=Stenotrophomonas maltophilia (strain R551-3) GN=trpB PE=3 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 4.544e-23
Identity = 28/34 (82.35%), Postives = 31/34 (91.18%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            D+DGQIIE HS+SAGLDYPGVGPEH+FL D GRA
Sbjct:  300 DDDGQIIETHSVSAGLDYPGVGPEHAFLADSGRA 333

HSP 2 Score: 63.1586 bits (152), Expect = 4.544e-23
Identity = 33/66 (50.00%), Postives = 41/66 (62.12%), Query Frame = 3 
Query:   96 GVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQT 293
            G   Y  +TD+EAL+AF   +  EGI+PALE++HALA   KL         V+ N SGRGDKDV T
Sbjct:  331 GRARYLGITDEEALQAFHLLAHTEGILPALESSHALAQAMKLARERPRDQIVLCNLSGRGDKDVHT 396
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_PSESM (Tryptophan synthase beta chain OS=Pseudomonas syringae pv. tomato GN=trpB PE=3 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 5.911e-23
Identity = 38/68 (55.88%), Postives = 48/68 (70.59%), Query Frame = 3 
Query:  105 EYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYL 308
            EY ++TD+EAL+AF +   LEGIIPALETAHALA   K    L D   +VV  SGRGDKD+QT + ++
Sbjct:  331 EYVSITDEEALDAFHQCCLLEGIIPALETAHALAEAMKRATNLRDDHLMVVCLSGRGDKDMQTVMNHM 398

HSP 2 Score: 53.1434 bits (126), Expect = 5.911e-23
Identity = 22/33 (66.67%), Postives = 27/33 (81.82%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            D DGQI + HSISAGLDYPG+GPEH++L +  R
Sbjct:  297 DNDGQITDAHSISAGLDYPGIGPEHAYLHEVKR 329
The following BLAST results are available for this feature:
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 410
Match NameE-valueIdentityDescription
TRPB_STAES2.684e-2353.03Tryptophan synthase beta chain OS=Staphylococcus e... [more]
TRPB_STAEQ2.684e-2353.03Tryptophan synthase beta chain OS=Staphylococcus e... [more]
TRPB_PSEPG3.492e-2355.88Tryptophan synthase beta chain OS=Pseudomonas puti... [more]
TRPB_LACCB4.544e-2346.48Tryptophan synthase beta chain OS=Lactobacillus ca... [more]
TRPB_LACCA4.544e-2346.48Tryptophan synthase beta chain OS=Lactobacillus ca... [more]
TRPB_LACC34.544e-2346.48Tryptophan synthase beta chain OS=Lactobacillus ca... [more]
TRPB_PARL14.544e-2352.05Tryptophan synthase beta chain OS=Parvibaculum lav... [more]
TRPB_RHOPS4.544e-2353.73Tryptophan synthase beta chain OS=Rhodopseudomonas... [more]
TRPB_STRM54.544e-2382.35Tryptophan synthase beta chain OS=Stenotrophomonas... [more]
TRPB_PSESM5.911e-2355.88Tryptophan synthase beta chain OS=Pseudomonas syri... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00970 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00970 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623890 ID=BQ623890; Name=BQ623890; organism=Citrus sinensis; type=EST; length=475bp
GGATGAAGATGGGCAAATAATTGAGCCACACTCCATTAGTGCAGGCTTAG
ATTACCCTGGAGTTGGACCTGAGCATAGTTTTCTGAAAGATGAAGGGCGT
GCGTGAGTACTATAATGTCACAGATGATGAAGCATTGGAAGCTTTCAAGA
GGTCATCACGGCTAGAGGGTATAATCCCAGCTTTGGAGACTGCTCATGCA
TTGGCTTATTTGGAGAAGCTATGCCCAACTCTTGCTGATGGTACTAAGGT
TGTGGTTAACTTCAGTGGCAGAGGTGACAAGGATGTCCAGACAGCGATCA
AATATTTGCAAGTTTGATGGTAATGCTTGTAACCCACAATATATGATTCA
TTTACTGAAAACAAATAAATGGGTTGCTTGAGCTTCTGTTTTTGAATTTT
GTATCATTAGTTATTTCAGAGATGTGAGAACCATCTTATGTAATTTGAGT
TAAAACTTTATATATTACATCTATT
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