BQ623890

Overview
NameBQ623890
Unique NameBQ623890
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length475
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB1_METAC (Tryptophan synthase beta chain 1 OS=Methanosarcina acetivorans GN=trpB1 PE=3 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 1.302e-22
Identity = 40/61 (65.57%), Postives = 48/61 (78.69%), Query Frame = 3 
Query:  117 VTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAI 299
            VTDDEAL+AF   SRLEGIIPALE++HALAYL+K   +   G  VVVN SGRGDKD++T +
Sbjct:  337 VTDDEALDAFNELSRLEGIIPALESSHALAYLKKAAESGELGEFVVVNLSGRGDKDLETVL 397

HSP 2 Score: 47.7506 bits (112), Expect = 1.302e-22
Identity = 20/33 (60.61%), Postives = 27/33 (81.82%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            D++GQI+E  S+SAGLDY GVGPE ++L + GR
Sbjct:  299 DKNGQILESESVSAGLDYSGVGPELAYLSESGR 331
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_PSEA6 (Tryptophan synthase beta chain OS=Pseudoalteromonas atlantica (strain T6c / BAA-1087) GN=trpB PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.303e-22
Identity = 38/64 (59.38%), Postives = 45/64 (70.31%), Query Frame = 3 
Query:   96 GVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDV 287
            G   Y  VTDDEAL AF+  SR EGIIPALE++HALA+   +     DGT +VVN SGRGDKD+
Sbjct:  319 GRATYAAVTDDEALYAFQLLSRKEGIIPALESSHALAHALNMADEAEDGTIIVVNLSGRGDKDL 382

HSP 2 Score: 53.9138 bits (128), Expect = 1.303e-22
Identity = 24/34 (70.59%), Postives = 28/34 (82.35%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            DEDGQI E +S+SAGLDYP VGP+H+ L D GRA
Sbjct:  288 DEDGQIEESYSVSAGLDYPAVGPQHAHLHDIGRA 321
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_STAS1 (Tryptophan synthase beta chain OS=Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) GN=trpB PE=3 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 1.694e-22
Identity = 32/69 (46.38%), Postives = 50/69 (72.46%), Query Frame = 3 
Query:  105 EYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYLQ 311
            +Y   +D +A++A  R ++ EGIIPA+E+AHAL+Y+E L PT+ +   +VV  SGRGDKD++T   Y++
Sbjct:  329 KYATASDKQAMDALVRFTKAEGIIPAIESAHALSYVETLAPTMRNDEILVVTVSGRGDKDMETIRNYMK 397

HSP 2 Score: 55.0694 bits (131), Expect = 1.694e-22
Identity = 25/33 (75.76%), Postives = 27/33 (81.82%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            D+DGQI   HSISAGLDYPGVGPEHS+  D GR
Sbjct:  295 DDDGQIQLAHSISAGLDYPGVGPEHSYYHDIGR 327
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_RHISN (Tryptophan synthase beta chain OS=Rhizobium sp. (strain NGR234) GN=trpB PE=3 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.694e-22
Identity = 36/68 (52.94%), Postives = 46/68 (67.65%), Query Frame = 3 
Query:  105 EYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYL 308
            EY  + D EALEAF+  +RLEGIIPALE +HALA + K  P +     +++N SGRGDKD+ T  K L
Sbjct:  335 EYVPIMDHEALEAFQTLTRLEGIIPALEPSHALAEVIKRAPKMGKDEIILMNLSGRGDKDIFTVGKIL 402

HSP 2 Score: 56.225 bits (134), Expect = 1.694e-22
Identity = 25/33 (75.76%), Postives = 28/33 (84.85%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            D DGQI E HSISAGLDYPG+GPEH++L D GR
Sbjct:  301 DGDGQIKEGHSISAGLDYPGIGPEHAWLNDIGR 333
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_RHIME (Tryptophan synthase beta chain OS=Rhizobium meliloti GN=trpB PE=3 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.694e-22
Identity = 36/68 (52.94%), Postives = 46/68 (67.65%), Query Frame = 3 
Query:  105 EYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYL 308
            EY  + D EALEAF+  +RLEGIIPALE +HALA + K  P +     +++N SGRGDKD+ T  K L
Sbjct:  335 EYVPIMDHEALEAFQTLTRLEGIIPALEPSHALAEVIKRAPKMGKDEIILMNLSGRGDKDIFTVGKIL 402

HSP 2 Score: 56.225 bits (134), Expect = 1.694e-22
Identity = 25/33 (75.76%), Postives = 28/33 (84.85%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            D DGQI E HSISAGLDYPG+GPEH++L D GR
Sbjct:  301 DGDGQIKEGHSISAGLDYPGIGPEHAWLNDIGR 333
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_RHILW (Tryptophan synthase beta chain OS=Rhizobium leguminosarum bv. trifolii (strain WSM2304) GN=trpB PE=3 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 1.694e-22
Identity = 34/68 (50.00%), Postives = 46/68 (67.65%), Query Frame = 3 
Query:  105 EYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYL 308
            +Y  + D EALEAF+  +RLEGIIPALE +HA+A + K  P +     +++N SGRGDKD+ T  K L
Sbjct:  335 DYVPIMDHEALEAFQTLTRLEGIIPALEPSHAIAEVIKRAPKMGKDEIILMNLSGRGDKDIFTVGKIL 402

HSP 2 Score: 58.151 bits (139), Expect = 1.694e-22
Identity = 26/33 (78.79%), Postives = 28/33 (84.85%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            D DGQI E HSISAGLDYPG+GPEHS+L D GR
Sbjct:  301 DGDGQIKEGHSISAGLDYPGIGPEHSWLNDTGR 333
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_DEIRA (Tryptophan synthase beta chain OS=Deinococcus radiodurans GN=trpB PE=3 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 2.202e-22
Identity = 37/72 (51.39%), Postives = 49/72 (68.06%), Query Frame = 3 
Query:   96 GVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYLQ 311
            G+ EY  VTD +ALE  +  +RLEGIIPALE+AHA++Y  +L   +     VVVN SGRGDKDV   ++ L+
Sbjct:  346 GMAEYVPVTDAQALEGLQLLTRLEGIIPALESAHAISYAVQLARQMKPEEIVVVNLSGRGDKDVTEVMRLLE 417

HSP 2 Score: 53.9138 bits (128), Expect = 2.202e-22
Identity = 22/34 (64.71%), Postives = 27/34 (79.41%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            D++GQI+ PHSISAGLDYPG+GPEH +    G A
Sbjct:  315 DDEGQIVPPHSISAGLDYPGIGPEHCYYSVMGMA 348
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_METNO (Tryptophan synthase beta chain OS=Methylobacterium nodulans (strain ORS2060 / LMG 21967) GN=trpB PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 2.204e-22
Identity = 37/67 (55.22%), Postives = 44/67 (65.67%), Query Frame = 3 
Query:  108 YYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYL 308
            Y + TD E LEAF+  S LEGIIPALE AHALA + +L PT      +VVN SGRGDKD+    + L
Sbjct:  336 YLSATDQETLEAFRLCSLLEGIIPALEPAHALAKVAELAPTKPRDHLMVVNLSGRGDKDIPQVAEIL 402

HSP 2 Score: 55.4546 bits (132), Expect = 2.204e-22
Identity = 23/33 (69.70%), Postives = 29/33 (87.88%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            +EDGQI + HSISAGLDYPG+GPEH++L + GR
Sbjct:  301 NEDGQIADAHSISAGLDYPGIGPEHAWLHEMGR 333
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_RHOP5 (Tryptophan synthase beta chain OS=Rhodopseudomonas palustris (strain BisA53) GN=trpB PE=3 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 2.204e-22
Identity = 35/67 (52.24%), Postives = 46/67 (68.66%), Query Frame = 3 
Query:  108 YYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYL 308
            + + TD EAL+AFK   RLEGIIPALE +HALA +  L P L     +V+N SGRGDKD+ +  ++L
Sbjct:  334 FLSATDTEALDAFKLCCRLEGIIPALEPSHALAKVADLAPKLPKDHLMVLNMSGRGDKDLASVAEHL 400

HSP 2 Score: 56.6102 bits (135), Expect = 2.204e-22
Identity = 25/33 (75.76%), Postives = 28/33 (84.85%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            D DGQI E HSISAGLDYPG+GPEH++L D GR
Sbjct:  299 DGDGQIQEAHSISAGLDYPGIGPEHAWLHDVGR 331
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_XANCP (Tryptophan synthase beta chain OS=Xanthomonas campestris pv. campestris GN=trpB PE=3 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 2.204e-22
Identity = 28/34 (82.35%), Postives = 31/34 (91.18%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            D+DGQIIE HS+SAGLDYPGVGPEH+FL D GRA
Sbjct:  300 DDDGQIIETHSVSAGLDYPGVGPEHAFLSDSGRA 333

HSP 2 Score: 60.4622 bits (145), Expect = 2.204e-22
Identity = 31/62 (50.00%), Postives = 39/62 (62.90%), Query Frame = 3 
Query:  108 YYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQT 293
            Y  +TDDEAL AF   +  EGI+ ALE++HA+A   KL   +     V+ N SGRGDKDV T
Sbjct:  335 YQGITDDEALAAFHLLAHTEGILAALESSHAVAQSIKLAREMPKDALVLCNLSGRGDKDVHT 396
The following BLAST results are available for this feature:
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 410
Match NameE-valueIdentityDescription
TRPB1_METAC1.302e-2265.57Tryptophan synthase beta chain 1 OS=Methanosarcina... [more]
TRPB_PSEA61.303e-2259.38Tryptophan synthase beta chain OS=Pseudoalteromona... [more]
TRPB_STAS11.694e-2246.38Tryptophan synthase beta chain OS=Staphylococcus s... [more]
TRPB_RHISN1.694e-2252.94Tryptophan synthase beta chain OS=Rhizobium sp. (s... [more]
TRPB_RHIME1.694e-2252.94Tryptophan synthase beta chain OS=Rhizobium melilo... [more]
TRPB_RHILW1.694e-2250.00Tryptophan synthase beta chain OS=Rhizobium legumi... [more]
TRPB_DEIRA2.202e-2251.39Tryptophan synthase beta chain OS=Deinococcus radi... [more]
TRPB_METNO2.204e-2255.22Tryptophan synthase beta chain OS=Methylobacterium... [more]
TRPB_RHOP52.204e-2252.24Tryptophan synthase beta chain OS=Rhodopseudomonas... [more]
TRPB_XANCP2.204e-2282.35Tryptophan synthase beta chain OS=Xanthomonas camp... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00970 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00970 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623890 ID=BQ623890; Name=BQ623890; organism=Citrus sinensis; type=EST; length=475bp
GGATGAAGATGGGCAAATAATTGAGCCACACTCCATTAGTGCAGGCTTAG
ATTACCCTGGAGTTGGACCTGAGCATAGTTTTCTGAAAGATGAAGGGCGT
GCGTGAGTACTATAATGTCACAGATGATGAAGCATTGGAAGCTTTCAAGA
GGTCATCACGGCTAGAGGGTATAATCCCAGCTTTGGAGACTGCTCATGCA
TTGGCTTATTTGGAGAAGCTATGCCCAACTCTTGCTGATGGTACTAAGGT
TGTGGTTAACTTCAGTGGCAGAGGTGACAAGGATGTCCAGACAGCGATCA
AATATTTGCAAGTTTGATGGTAATGCTTGTAACCCACAATATATGATTCA
TTTACTGAAAACAAATAAATGGGTTGCTTGAGCTTCTGTTTTTGAATTTT
GTATCATTAGTTATTTCAGAGATGTGAGAACCATCTTATGTAATTTGAGT
TAAAACTTTATATATTACATCTATT
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