BQ623890

Overview
NameBQ623890
Unique NameBQ623890
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length475
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_LEPBA (Tryptophan synthase beta chain OS=Leptospira biflexa serovar Patoc (strain Patoc 1 / Ames) GN=trpB PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 9.943e-19
Identity = 30/68 (44.12%), Postives = 42/68 (61.76%), Query Frame = 3 
Query:  105 EYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYL 308
            +Y  VTD++AL+ F   +R+EGIIPALETAHA      +   L     +++  SGRGDKDV   ++ L
Sbjct:  324 DYRMVTDEQALDCFLEVTRVEGIIPALETAHAFYVARDVAKDLGKKKDLIICLSGRGDKDVTEVLRIL 391

HSP 2 Score: 52.373 bits (124), Expect = 9.943e-19
Identity = 22/33 (66.67%), Postives = 26/33 (78.79%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            DE GQI+  HS+SAGLDYPGVGPEH++L    R
Sbjct:  290 DEAGQIVPAHSVSAGLDYPGVGPEHAYLSQTKR 322
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_BUCBP (Tryptophan synthase beta chain OS=Buchnera aphidicola subsp. Baizongia pistaciae GN=trpB PE=3 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 9.944e-19
Identity = 36/70 (51.43%), Postives = 47/70 (67.14%), Query Frame = 3 
Query:   96 GVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTK-VVVNFSGRGDKDVQTAIK 302
            G   Y ++TD EA+ AFK   +LEGIIPALE++HALAY  KL     +  + ++VN SGRGDKD+ T  K
Sbjct:  319 GRATYVSITDCEAINAFKILCKLEGIIPALESSHALAYALKLISKDTNKKQIIIVNISGRGDKDIDTIRK 388

HSP 2 Score: 46.9802 bits (110), Expect = 9.944e-19
Identity = 22/32 (68.75%), Postives = 26/32 (81.25%), Query Frame = 2 
Query:    8 DGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            +GQI E  SISAGLD+P VGPEH++LK  GRA
Sbjct:  290 EGQIKESWSISAGLDFPAVGPEHAWLKHIGRA 321
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_ERWT9 (Tryptophan synthase beta chain OS=Erwinia tasmaniensis (strain DSM 17950 / Et1/99) GN=trpB PE=3 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.681e-18
Identity = 35/67 (52.24%), Postives = 47/67 (70.15%), Query Frame = 3 
Query:   96 GVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTK-VVVNFSGRGDKDVQT 293
            G   Y ++TD+EAL+AFK   R EGIIPALE++HALA+  K+     +  + +VVN SGRGDKD+ T
Sbjct:  319 GRANYVSITDEEALDAFKALCRSEGIIPALESSHALAHALKMMREAPEKEQLLVVNLSGRGDKDIFT 385

HSP 2 Score: 46.595 bits (109), Expect = 1.681e-18
Identity = 21/32 (65.62%), Postives = 25/32 (78.12%), Query Frame = 2 
Query:    8 DGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            DGQI E +SISAGLD+P VGP+H+ L   GRA
Sbjct:  290 DGQIEESYSISAGLDFPSVGPQHAHLNSIGRA 321
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_BUCMH (Tryptophan synthase beta chain OS=Buchnera aphidicola subsp. Melaphis rhois GN=trpB PE=3 SV=2)

HSP 1 Score: 68.5514 bits (166), Expect = 1.682e-18
Identity = 34/71 (47.89%), Postives = 50/71 (70.42%), Query Frame = 3 
Query:  105 EYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKV-VVNFSGRGDKDVQTAIKYLQV 314
            +Y ++TD EA+ AF+  S+ EGIIPALE++HALAY  KL        ++ +VN SGRGDKD++T   +L++
Sbjct:  322 QYVSITDHEAVNAFRCLSKSEGIIPALESSHALAYALKLMNNHPQKNQILIVNISGRGDKDIETVKNFLKM 392

HSP 2 Score: 44.2838 bits (103), Expect = 1.682e-18
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:    5 EDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            E+GQI E  SISAGLD+P VGPEH++L    RA
Sbjct:  289 EEGQIKESWSISAGLDFPAVGPEHAWLYSTKRA 321
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_BUCSC (Tryptophan synthase beta chain OS=Buchnera aphidicola subsp. Schlechtendalia chinensis GN=trpB PE=3 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 2.186e-18
Identity = 36/68 (52.94%), Postives = 50/68 (73.53%), Query Frame = 3 
Query:  108 YYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTK-VVVNFSGRGDKDVQTAIKYL 308
            Y ++TD EA+ AF+  S+LEGIIPALE++HALAY  KL     +  + ++VN SGRGDKD++T  K+L
Sbjct:  323 YVSITDSEAVHAFQHLSKLEGIIPALESSHALAYAIKLMNNYPNKNQTLIVNISGRGDKDLKTVEKFL 390

HSP 2 Score: 40.817 bits (94), Expect = 2.186e-18
Identity = 18/26 (69.23%), Postives = 22/26 (84.62%), Query Frame = 2 
Query:    8 DGQIIEPHSISAGLDYPGVGPEHSFL 85
            +GQI E  SISAGLD+P VGPEH++L
Sbjct:  290 EGQIKESWSISAGLDFPAVGPEHAWL 315
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_PARD8 (Tryptophan synthase beta chain OS=Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / NCTC 11152) GN=trpB PE=3 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.186e-18
Identity = 36/63 (57.14%), Postives = 41/63 (65.08%), Query Frame = 3 
Query:  117 VTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKY 305
            V DDEALEA    +RLEGIIPALE+AHAL  L K+         VV+  SGRGDKD+ T I Y
Sbjct:  331 VDDDEALEAAFELTRLEGIIPALESAHALGALAKV--NFKPEEVVVLTVSGRGDKDMDTYINY 391

HSP 2 Score: 50.8322 bits (120), Expect = 2.186e-18
Identity = 23/37 (62.16%), Postives = 30/37 (81.08%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA*VL 112
            +EDGQI EP+S+SAGLDYPG+GP H+ L  + RA +L
Sbjct:  293 NEDGQIEEPYSVSAGLDYPGIGPIHANLSVKHRAQIL 329
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_CORGL (Tryptophan synthase beta chain OS=Corynebacterium glutamicum GN=trpB PE=3 SV=2)

HSP 1 Score: 62.003 bits (149), Expect = 2.839e-18
Identity = 34/75 (45.33%), Postives = 46/75 (61.33%), Query Frame = 3 
Query:   96 GVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLAD---GTKVVVNFSGRGDKDVQTAIKYLQ 311
            G   Y  +TD EAL+AF+  +R EGIIPALE++HA AY  K   T  +      ++V+ SGRGDKDV    + L+
Sbjct:  332 GRATYVGITDAEALQAFQYLARYEGIIPALESSHAFAYALKRAKTAEEEGQNLTILVSLSGRGDKDVDHVRRTLE 406

HSP 2 Score: 50.0618 bits (118), Expect = 2.839e-18
Identity = 22/34 (64.71%), Postives = 27/34 (79.41%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            + DGQ+ E +SISAGLDYPGVGP+H+ L   GRA
Sbjct:  301 NSDGQVEESYSISAGLDYPGVGPQHAHLHATGRA 334
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_CORGB (Tryptophan synthase beta chain OS=Corynebacterium glutamicum (strain R) GN=trpB PE=3 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 2.839e-18
Identity = 34/75 (45.33%), Postives = 46/75 (61.33%), Query Frame = 3 
Query:   96 GVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLAD---GTKVVVNFSGRGDKDVQTAIKYLQ 311
            G   Y  +TD EAL+AF+  +R EGIIPALE++HA AY  K   T  +      ++V+ SGRGDKDV    + L+
Sbjct:  332 GRATYVGITDAEALQAFQYLARYEGIIPALESSHAFAYALKRAKTAEEEGQNLTILVSLSGRGDKDVDHVRRTLE 406

HSP 2 Score: 50.0618 bits (118), Expect = 2.839e-18
Identity = 22/34 (64.71%), Postives = 27/34 (79.41%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            + DGQ+ E +SISAGLDYPGVGP+H+ L   GRA
Sbjct:  301 NSDGQVEESYSISAGLDYPGVGPQHAHLHATGRA 334
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_ELUMP (Tryptophan synthase beta chain OS=Elusimicrobium minutum (strain Pei191) GN=trpB PE=3 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 2.840e-18
Identity = 35/67 (52.24%), Postives = 44/67 (65.67%), Query Frame = 3 
Query:   87 KMKGVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDV 287
            K  G   Y +  DDEA++AF   SR EGIIPALE+AHA+AY  KL   L     ++VN SGRG+KD+
Sbjct:  331 KDSGRVTYRSACDDEAVDAFFMLSRKEGIIPALESAHAVAYALKLSKELGKDKSILVNLSGRGEKDL 397

HSP 2 Score: 45.4394 bits (106), Expect = 2.840e-18
Identity = 18/33 (54.55%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            D+ G  +  HS+++GLDY GVGP+H +LKD GR
Sbjct:  303 DDKGNPMGTHSVASGLDYLGVGPQHCYLKDSGR 335
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_FRATN (Tryptophan synthase beta chain OS=Francisella tularensis subsp. novicida (strain U112) GN=trpB PE=3 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 2.843e-18
Identity = 39/69 (56.52%), Postives = 47/69 (68.12%), Query Frame = 3 
Query:   96 GVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTK---VVVNFSGRGDKDVQT 293
            G  EY + TDDEAL+AFK   + EGIIPALE++HALA+  KL     D  K   +VVN SGRGDKD+ T
Sbjct:  319 GRAEYASATDDEALDAFKLLCKKEGIIPALESSHALAHALKL--AYEDPNKEQLLVVNLSGRGDKDIFT 385

HSP 2 Score: 45.4394 bits (106), Expect = 2.843e-18
Identity = 21/34 (61.76%), Postives = 26/34 (76.47%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            + DGQI E +SISAGLD+P VGP+H+ L   GRA
Sbjct:  288 NSDGQIEESYSISAGLDFPSVGPQHAHLLAIGRA 321
The following BLAST results are available for this feature:
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 410
Match NameE-valueIdentityDescription
TRPB_LEPBA9.943e-1944.12Tryptophan synthase beta chain OS=Leptospira bifle... [more]
TRPB_BUCBP9.944e-1951.43Tryptophan synthase beta chain OS=Buchnera aphidic... [more]
TRPB_ERWT91.681e-1852.24Tryptophan synthase beta chain OS=Erwinia tasmanie... [more]
TRPB_BUCMH1.682e-1847.89Tryptophan synthase beta chain OS=Buchnera aphidic... [more]
TRPB_BUCSC2.186e-1852.94Tryptophan synthase beta chain OS=Buchnera aphidic... [more]
TRPB_PARD82.186e-1857.14Tryptophan synthase beta chain OS=Parabacteroides ... [more]
TRPB_CORGL2.839e-1845.33Tryptophan synthase beta chain OS=Corynebacterium ... [more]
TRPB_CORGB2.839e-1845.33Tryptophan synthase beta chain OS=Corynebacterium ... [more]
TRPB_ELUMP2.840e-1852.24Tryptophan synthase beta chain OS=Elusimicrobium m... [more]
TRPB_FRATN2.843e-1856.52Tryptophan synthase beta chain OS=Francisella tula... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00970 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00970 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623890 ID=BQ623890; Name=BQ623890; organism=Citrus sinensis; type=EST; length=475bp
GGATGAAGATGGGCAAATAATTGAGCCACACTCCATTAGTGCAGGCTTAG
ATTACCCTGGAGTTGGACCTGAGCATAGTTTTCTGAAAGATGAAGGGCGT
GCGTGAGTACTATAATGTCACAGATGATGAAGCATTGGAAGCTTTCAAGA
GGTCATCACGGCTAGAGGGTATAATCCCAGCTTTGGAGACTGCTCATGCA
TTGGCTTATTTGGAGAAGCTATGCCCAACTCTTGCTGATGGTACTAAGGT
TGTGGTTAACTTCAGTGGCAGAGGTGACAAGGATGTCCAGACAGCGATCA
AATATTTGCAAGTTTGATGGTAATGCTTGTAACCCACAATATATGATTCA
TTTACTGAAAACAAATAAATGGGTTGCTTGAGCTTCTGTTTTTGAATTTT
GTATCATTAGTTATTTCAGAGATGTGAGAACCATCTTATGTAATTTGAGT
TAAAACTTTATATATTACATCTATT
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