BQ623890

Overview
NameBQ623890
Unique NameBQ623890
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length475
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_RUTMC (Tryptophan synthase beta chain OS=Ruthia magnifica subsp. Calyptogena magnifica GN=trpB PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 2.342e-25
Identity = 34/69 (49.28%), Postives = 46/69 (66.67%), Query Frame = 3 
Query:   87 KMKGVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQT 293
            K  G   Y  +TD EALEAF   +++EGI+PALE++HA+AY  KL   L     +++N SGRGDKD+ T
Sbjct:  324 KDSGRARYVAITDKEALEAFHVLTKVEGILPALESSHAVAYGIKLAKELGKDKHIIINLSGRGDKDIHT 392

HSP 2 Score: 65.4698 bits (158), Expect = 2.342e-25
Identity = 29/34 (85.29%), Postives = 32/34 (94.12%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            DE+GQIIE HSISAGLDYPGVGPEH++LKD GRA
Sbjct:  296 DENGQIIEGHSISAGLDYPGVGPEHAYLKDSGRA 329
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_CHRVO (Tryptophan synthase beta chain OS=Chromobacterium violaceum GN=trpB PE=3 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 2.343e-25
Identity = 35/66 (53.03%), Postives = 46/66 (69.70%), Query Frame = 3 
Query:   96 GVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQT 293
            G  EY ++ DDEAL AF     LEGIIPALE++HALA+  K+ P++     ++VN SGRGDKD+ T
Sbjct:  326 GRAEYVSIDDDEALRAFHDCCHLEGIIPALESSHALAWAAKVAPSMGKDQVILVNLSGRGDKDINT 391

HSP 2 Score: 61.2326 bits (147), Expect = 2.343e-25
Identity = 27/34 (79.41%), Postives = 29/34 (85.29%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            D DGQI+E HS+SAGLDYPGVGPEH  LKD GRA
Sbjct:  295 DADGQIVETHSVSAGLDYPGVGPEHCHLKDIGRA 328
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_BACCQ (Tryptophan synthase beta chain OS=Bacillus cereus (strain Q1) GN=trpB PE=3 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 3.049e-25
Identity = 35/68 (51.47%), Postives = 52/68 (76.47%), Query Frame = 3 
Query:  108 YYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYLQ 311
            Y+++TDDEALEAF+  ++ EGIIPALE++HA+AY  KL P +     +V+  SGRGDKDV++  +Y++
Sbjct:  328 YHSITDDEALEAFQLLTKKEGIIPALESSHAVAYALKLAPQMKKDEGLVICLSGRGDKDVESIKRYME 395

HSP 2 Score: 58.9214 bits (141), Expect = 3.049e-25
Identity = 27/33 (81.82%), Postives = 29/33 (87.88%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            +E+GQI E HSISAGLDYPGVGPEHS LKD GR
Sbjct:  293 NEEGQIQEAHSISAGLDYPGVGPEHSLLKDIGR 325
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_BACC7 (Tryptophan synthase beta chain OS=Bacillus cereus (strain AH187) GN=trpB PE=3 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 3.049e-25
Identity = 35/68 (51.47%), Postives = 52/68 (76.47%), Query Frame = 3 
Query:  108 YYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYLQ 311
            Y+++TDDEALEAF+  ++ EGIIPALE++HA+AY  KL P +     +V+  SGRGDKDV++  +Y++
Sbjct:  328 YHSITDDEALEAFQLLTKKEGIIPALESSHAVAYALKLAPQMKKDEGLVICLSGRGDKDVESIKRYME 395

HSP 2 Score: 58.9214 bits (141), Expect = 3.049e-25
Identity = 27/33 (81.82%), Postives = 29/33 (87.88%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            +E+GQI E HSISAGLDYPGVGPEHS LKD GR
Sbjct:  293 NEEGQIQEAHSISAGLDYPGVGPEHSLLKDIGR 325
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_BACC2 (Tryptophan synthase beta chain OS=Bacillus cereus (strain G9842) GN=trpB PE=3 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 3.049e-25
Identity = 35/68 (51.47%), Postives = 52/68 (76.47%), Query Frame = 3 
Query:  108 YYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIKYLQ 311
            Y+++TDDEALEAF+  ++ EGIIPALE++HA+AY  KL P +     +V+  SGRGDKDV++  +Y++
Sbjct:  328 YHSITDDEALEAFQLLTKKEGIIPALESSHAVAYALKLAPKMKKDEGLVICLSGRGDKDVESIKRYME 395

HSP 2 Score: 58.9214 bits (141), Expect = 3.049e-25
Identity = 27/33 (81.82%), Postives = 29/33 (87.88%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            +E+GQI E HSISAGLDYPGVGPEHS LKD GR
Sbjct:  293 NEEGQIQEAHSISAGLDYPGVGPEHSLLKDIGR 325
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB1_PYRKO (Tryptophan synthase beta chain 1 OS=Pyrococcus kodakaraensis GN=trpB1 PE=3 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 3.050e-25
Identity = 37/67 (55.22%), Postives = 46/67 (68.66%), Query Frame = 3 
Query:   87 KMKGVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDV 287
            K  G  EY  VTD+EAL AF   SR EGI+PALE+AHA+AY  K+ P +     ++VN SGRGDKD+
Sbjct:  314 KDSGRAEYVTVTDEEALRAFHELSRTEGILPALESAHAVAYAMKIAPEMDKDEIIIVNLSGRGDKDL 380

HSP 2 Score: 59.6918 bits (143), Expect = 3.050e-25
Identity = 25/34 (73.53%), Postives = 30/34 (88.24%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            +E+GQI   HS+SAGLDYPGVGPEH++LKD GRA
Sbjct:  286 NEEGQITPTHSVSAGLDYPGVGPEHAYLKDSGRA 319
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_POLSJ (Tryptophan synthase beta chain OS=Polaromonas sp. (strain JS666 / ATCC BAA-500) GN=trpB PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 3.960e-25
Identity = 38/71 (53.52%), Postives = 46/71 (64.79%), Query Frame = 3 
Query:   81 F*KMKGVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQT 293
            F K  G  EY  +TD EALEAF    R EGII ALE++HA+AY  KL  T+     ++VN SGRGDKD+ T
Sbjct:  321 FLKDIGRAEYVGITDTEALEAFHYLCRTEGIIAALESSHAVAYAMKLAKTMRSDQSILVNLSGRGDKDIGT 391

HSP 2 Score: 62.3882 bits (150), Expect = 3.960e-25
Identity = 27/34 (79.41%), Postives = 31/34 (91.18%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            ++DGQ+ E HSISAGLDYPGVGPEH+FLKD GRA
Sbjct:  295 NDDGQVTETHSISAGLDYPGVGPEHAFLKDIGRA 328
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_ACICA (Tryptophan synthase beta chain OS=Acinetobacter calcoaceticus GN=trpB PE=3 SV=2)

HSP 1 Score: 75.0998 bits (183), Expect = 3.967e-25
Identity = 33/66 (50.00%), Postives = 48/66 (72.73%), Query Frame = 3 
Query:  105 EYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIK 302
            EY  + D+EAL+ F+  +R+EGIIPA+E+AHA+AY+ KL PT+     ++ N SGRGDKD+ T  +
Sbjct:  329 EYVPIDDNEALQGFRDLTRIEGIIPAIESAHAMAYVTKLAPTMDKDQIIIANVSGRGDKDLMTVAR 394

HSP 2 Score: 60.077 bits (144), Expect = 3.967e-25
Identity = 28/33 (84.85%), Postives = 28/33 (84.85%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            D  GQIIE HSISAGLDYPGVGPEHSFLKD  R
Sbjct:  295 DPQGQIIETHSISAGLDYPGVGPEHSFLKDMHR 327
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_ACIAD (Tryptophan synthase beta chain OS=Acinetobacter sp. (strain ADP1) GN=trpB PE=3 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 3.967e-25
Identity = 33/66 (50.00%), Postives = 48/66 (72.73%), Query Frame = 3 
Query:  105 EYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQTAIK 302
            EY  + D+EAL+ F+  +R+EGIIPA+E+AHA+AY+ KL PT+     ++ N SGRGDKD+ T  +
Sbjct:  329 EYVPIDDNEALQGFRDLTRIEGIIPAIESAHAMAYVTKLAPTMDKDQIIIANVSGRGDKDLMTVAR 394

HSP 2 Score: 60.077 bits (144), Expect = 3.967e-25
Identity = 28/33 (84.85%), Postives = 28/33 (84.85%), Query Frame = 2 
Query:    2 DEDGQIIEPHSISAGLDYPGVGPEHSFLKDEGR 100
            D  GQIIE HSISAGLDYPGVGPEHSFLKD  R
Sbjct:  295 DPQGQIIETHSISAGLDYPGVGPEHSFLKDMHR 327
[Close]
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Match: TRPB_BORPA (Tryptophan synthase beta chain OS=Bordetella parapertussis GN=trpB PE=3 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 3.968e-25
Identity = 37/69 (53.62%), Postives = 47/69 (68.12%), Query Frame = 3 
Query:   87 KMKGVREYYNVTDDEALEAFKRSSRLEGIIPALETAHALAYLEKLCPTLADGTKVVVNFSGRGDKDVQT 293
            K  G  EY  +TDDEAL AF    R+EGI+PALE+AHA+A   K+ P L+    ++VN SGRGDKD+ T
Sbjct:  322 KDSGRAEYAGITDDEALAAFHDCCRIEGIMPALESAHAIAQAVKMAPALSKDKVILVNLSGRGDKDMHT 390

HSP 2 Score: 59.3066 bits (142), Expect = 3.968e-25
Identity = 25/32 (78.12%), Postives = 29/32 (90.62%), Query Frame = 2 
Query:    8 DGQIIEPHSISAGLDYPGVGPEHSFLKDEGRA 103
            DGQ+ E HS+SAGLDYPGVGPEH++LKD GRA
Sbjct:  296 DGQVQETHSVSAGLDYPGVGPEHAWLKDSGRA 327
The following BLAST results are available for this feature:
BLAST of BQ623890 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 410
Match NameE-valueIdentityDescription
TRPB_RUTMC2.342e-2549.28Tryptophan synthase beta chain OS=Ruthia magnifica... [more]
TRPB_CHRVO2.343e-2553.03Tryptophan synthase beta chain OS=Chromobacterium ... [more]
TRPB_BACCQ3.049e-2551.47Tryptophan synthase beta chain OS=Bacillus cereus ... [more]
TRPB_BACC73.049e-2551.47Tryptophan synthase beta chain OS=Bacillus cereus ... [more]
TRPB_BACC23.049e-2551.47Tryptophan synthase beta chain OS=Bacillus cereus ... [more]
TRPB1_PYRKO3.050e-2555.22Tryptophan synthase beta chain 1 OS=Pyrococcus kod... [more]
TRPB_POLSJ3.960e-2553.52Tryptophan synthase beta chain OS=Polaromonas sp. ... [more]
TRPB_ACICA3.967e-2550.00Tryptophan synthase beta chain OS=Acinetobacter ca... [more]
TRPB_ACIAD3.967e-2550.00Tryptophan synthase beta chain OS=Acinetobacter sp... [more]
TRPB_BORPA3.968e-2553.62Tryptophan synthase beta chain OS=Bordetella parap... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00970 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00970 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623890 ID=BQ623890; Name=BQ623890; organism=Citrus sinensis; type=EST; length=475bp
GGATGAAGATGGGCAAATAATTGAGCCACACTCCATTAGTGCAGGCTTAG
ATTACCCTGGAGTTGGACCTGAGCATAGTTTTCTGAAAGATGAAGGGCGT
GCGTGAGTACTATAATGTCACAGATGATGAAGCATTGGAAGCTTTCAAGA
GGTCATCACGGCTAGAGGGTATAATCCCAGCTTTGGAGACTGCTCATGCA
TTGGCTTATTTGGAGAAGCTATGCCCAACTCTTGCTGATGGTACTAAGGT
TGTGGTTAACTTCAGTGGCAGAGGTGACAAGGATGTCCAGACAGCGATCA
AATATTTGCAAGTTTGATGGTAATGCTTGTAACCCACAATATATGATTCA
TTTACTGAAAACAAATAAATGGGTTGCTTGAGCTTCTGTTTTTGAATTTT
GTATCATTAGTTATTTCAGAGATGTGAGAACCATCTTATGTAATTTGAGT
TAAAACTTTATATATTACATCTATT
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