CB304622

Overview
NameCB304622
Unique NameCB304622
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length608
Libraries
Library NameType
Flavedo Maturecdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CB304622 vs. ExPASy Swiss-Prot
Match: PIP21_MAIZE (Aquaporin PIP2-1 OS=Zea mays GN=PIP2-1 PE=1 SV=2)

HSP 1 Score: 123.635 bits (309), Expect = 2.581e-49
Identity = 55/63 (87.30%), Postives = 60/63 (95.24%), Query Frame = -1
Query:  276 LATIPITGTGINPARSLGAAVIYNKDKVWDDQWLFWVGPFIGAAIAAFYHQFILRAGAVKALG 464
            LATIP+TGTGINPARSLGAAVIYNKDK WDD W+FWVGP +GAAIAAFYHQ+ILRAGA+KALG
Sbjct:  222 LATIPVTGTGINPARSLGAAVIYNKDKPWDDHWIFWVGPLVGAAIAAFYHQYILRAGAIKALG 284          

HSP 2 Score: 93.2041 bits (230), Expect = 2.581e-49
Identity = 45/47 (95.74%), Postives = 46/47 (97.87%), Query Frame = -2
Query:  464 GLAAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVH 604
            GL AEIIGTFVLVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVFMVH
Sbjct:  175 GLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVH 221          
BLAST of CB304622 vs. ExPASy Swiss-Prot
Match: PIP25_ARATH (Probable aquaporin PIP2-5 OS=Arabidopsis thaliana GN=PIP2-5 PE=1 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 3.366e-49
Identity = 55/63 (87.30%), Postives = 59/63 (93.65%), Query Frame = -1
Query:  276 LATIPITGTGINPARSLGAAVIYNKDKVWDDQWLFWVGPFIGAAIAAFYHQFILRAGAVKALG 464
            LATIPITGTGINPARSLGAA+IYNKDK WD  W+FWVGPF GAAIAAFYHQF+LRAGA+KALG
Sbjct:  216 LATIPITGTGINPARSLGAAIIYNKDKAWDHHWIFWVGPFAGAAIAAFYHQFVLRAGAIKALG 278          

HSP 2 Score: 93.2041 bits (230), Expect = 3.366e-49
Identity = 45/47 (95.74%), Postives = 47/47 (100.00%), Query Frame = -2
Query:  464 GLAAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVH 604
            G+AAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVF+VH
Sbjct:  169 GVAAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVH 215          
BLAST of CB304622 vs. ExPASy Swiss-Prot
Match: PIP22_MAIZE (Aquaporin PIP2-2 OS=Zea mays GN=PIP2-2 PE=2 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 5.716e-49
Identity = 54/63 (85.71%), Postives = 60/63 (95.24%), Query Frame = -1
Query:  276 LATIPITGTGINPARSLGAAVIYNKDKVWDDQWLFWVGPFIGAAIAAFYHQFILRAGAVKALG 464
            LATIP+TGTGINPARSLGAAV+YNKDK WDD W+FWVGP +GAAIAAFYHQ+ILRAGA+KALG
Sbjct:  224 LATIPVTGTGINPARSLGAAVVYNKDKPWDDHWIFWVGPLLGAAIAAFYHQYILRAGAIKALG 286          

HSP 2 Score: 92.8189 bits (229), Expect = 5.716e-49
Identity = 44/47 (93.62%), Postives = 46/47 (97.87%), Query Frame = -2
Query:  464 GLAAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVH 604
            GL AEI+GTFVLVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVFMVH
Sbjct:  177 GLGAEIVGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVH 223          
BLAST of CB304622 vs. ExPASy Swiss-Prot
Match: PIP26_ARATH (Probable aquaporin PIP2-6 OS=Arabidopsis thaliana GN=PIP2-6 PE=1 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 2.803e-48
Identity = 54/63 (85.71%), Postives = 58/63 (92.06%), Query Frame = -1
Query:  276 LATIPITGTGINPARSLGAAVIYNKDKVWDDQWLFWVGPFIGAAIAAFYHQFILRAGAVKALG 464
            LATIPITGTGINPARS GAAVIYN  K WDDQW+FWVGPF+GAAIAAFYHQF+LRAGA+KA G
Sbjct:  216 LATIPITGTGINPARSFGAAVIYNNQKAWDDQWIFWVGPFVGAAIAAFYHQFVLRAGAMKAYG 278          

HSP 2 Score: 92.0485 bits (227), Expect = 2.803e-48
Identity = 43/48 (89.58%), Postives = 47/48 (97.92%), Query Frame = -2
Query:  464 VGLAAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVH 607
            VG+ AEIIGTFVLVYTVFSATDPKR+ARDSH+PVLAPLPIGF+VFMVH
Sbjct:  168 VGVGAEIIGTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFSVFMVH 215          
BLAST of CB304622 vs. ExPASy Swiss-Prot
Match: PIP21_ARATH (Aquaporin PIP2-1 OS=Arabidopsis thaliana GN=PIP2-1 PE=1 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 4.762e-48
Identity = 53/63 (84.13%), Postives = 56/63 (88.89%), Query Frame = -1
Query:  276 LATIPITGTGINPARSLGAAVIYNKDKVWDDQWLFWVGPFIGAAIAAFYHQFILRAGAVKALG 464
            LATIPITGTGINPARS GAAVIYNK K WDD W+FWVGPFIGAAIAAFYHQF+LRA   K+LG
Sbjct:  217 LATIPITGTGINPARSFGAAVIYNKSKPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLG 279          

HSP 2 Score: 95.9005 bits (237), Expect = 4.762e-48
Identity = 47/47 (100.00%), Postives = 47/47 (100.00%), Query Frame = -2
Query:  464 GLAAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVH 604
            GLAAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVH
Sbjct:  170 GLAAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVH 216          
BLAST of CB304622 vs. ExPASy Swiss-Prot
Match: PIP24_ARATH (Probable aquaporin PIP2-4 OS=Arabidopsis thaliana GN=PIP2-4 PE=1 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 6.205e-48
Identity = 54/63 (85.71%), Postives = 57/63 (90.48%), Query Frame = -1
Query:  276 LATIPITGTGINPARSLGAAVIYNKDKVWDDQWLFWVGPFIGAAIAAFYHQFILRAGAVKALG 464
            LATIPITGTGINPARS GAAVIYN +K WDDQW+FWVGP IGAA AAFYHQFILRA A+KALG
Sbjct:  217 LATIPITGTGINPARSFGAAVIYNNEKAWDDQWIFWVGPMIGAAAAAFYHQFILRAAAIKALG 279          

HSP 2 Score: 93.2041 bits (230), Expect = 6.205e-48
Identity = 45/47 (95.74%), Postives = 46/47 (97.87%), Query Frame = -2
Query:  464 GLAAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVH 604
            GL AEIIGTFVLVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVFMVH
Sbjct:  170 GLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVH 216          
BLAST of CB304622 vs. ExPASy Swiss-Prot
Match: PIP21_ORYSJ (Probable aquaporin PIP2-1 OS=Oryza sativa subsp. japonica GN=PIP2-1 PE=2 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 1.054e-47
Identity = 51/63 (80.95%), Postives = 59/63 (93.65%), Query Frame = -1
Query:  276 LATIPITGTGINPARSLGAAVIYNKDKVWDDQWLFWVGPFIGAAIAAFYHQFILRAGAVKALG 464
            LATIPITGTGINPARS+GAAVI+N +K W + W+FWVGPF+GAAIAAFYHQ+ILRAGA+KALG
Sbjct:  222 LATIPITGTGINPARSIGAAVIFNNEKAWHNHWIFWVGPFVGAAIAAFYHQYILRAGAIKALG 284          

HSP 2 Score: 94.7449 bits (234), Expect = 1.054e-47
Identity = 46/47 (97.87%), Postives = 47/47 (100.00%), Query Frame = -2
Query:  464 GLAAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVH 604
            GLAAEIIGTFVLVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVFMVH
Sbjct:  175 GLAAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVH 221          
BLAST of CB304622 vs. ExPASy Swiss-Prot
Match: PIP22_ARATH (Aquaporin PIP2-2 OS=Arabidopsis thaliana GN=PIP2-2 PE=1 SV=2)

HSP 1 Score: 116.701 bits (291), Expect = 1.054e-47
Identity = 53/63 (84.13%), Postives = 56/63 (88.89%), Query Frame = -1
Query:  276 LATIPITGTGINPARSLGAAVIYNKDKVWDDQWLFWVGPFIGAAIAAFYHQFILRAGAVKALG 464
            LATIPITGTGINPARS GAAVIYNK K WDD W+FWVGPFIGAAIAAFYHQF+LRA   K+LG
Sbjct:  215 LATIPITGTGINPARSFGAAVIYNKSKPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLG 277          

HSP 2 Score: 94.7449 bits (234), Expect = 1.054e-47
Identity = 46/47 (97.87%), Postives = 47/47 (100.00%), Query Frame = -2
Query:  464 GLAAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVH 604
            GLAAEIIGTFVLVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVFMVH
Sbjct:  168 GLAAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVH 214          
BLAST of CB304622 vs. ExPASy Swiss-Prot
Match: PIP24_MAIZE (Aquaporin PIP2-4 OS=Zea mays GN=PIP2-4 PE=1 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 1.791e-47
Identity = 52/60 (86.67%), Postives = 55/60 (91.67%), Query Frame = -1
Query:  285 LATIPITGTGINPARSLGAAVIYNKDKVWDDQWLFWVGPFIGAAIAAFYHQFILRAGAVK 464
            LATIPITGTGINPARSLGAAVIYNKDK WDDQW+FWVGP IGAAIAA YHQ++LRA A K
Sbjct:  221 LATIPITGTGINPARSLGAAVIYNKDKAWDDQWIFWVGPLIGAAIAAAYHQYVLRASATK 280          

HSP 2 Score: 95.9005 bits (237), Expect = 1.791e-47
Identity = 47/47 (100.00%), Postives = 47/47 (100.00%), Query Frame = -2
Query:  464 GLAAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVH 604
            GLAAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVH
Sbjct:  174 GLAAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVH 220          
BLAST of CB304622 vs. ExPASy Swiss-Prot
Match: PIP23_MAIZE (Aquaporin PIP2-3 OS=Zea mays GN=PIP2-3 PE=2 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 1.791e-47
Identity = 52/60 (86.67%), Postives = 55/60 (91.67%), Query Frame = -1
Query:  285 LATIPITGTGINPARSLGAAVIYNKDKVWDDQWLFWVGPFIGAAIAAFYHQFILRAGAVK 464
            LATIPITGTGINPARSLGAAVIYNKDK WDDQW+FWVGP IGAAIAA YHQ++LRA A K
Sbjct:  222 LATIPITGTGINPARSLGAAVIYNKDKAWDDQWIFWVGPLIGAAIAAAYHQYVLRASATK 281          

HSP 2 Score: 95.9005 bits (237), Expect = 1.791e-47
Identity = 47/47 (100.00%), Postives = 47/47 (100.00%), Query Frame = -2
Query:  464 GLAAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVH 604
            GLAAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVH
Sbjct:  175 GLAAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVH 221          
The following BLAST results are available for this feature:
BLAST of CB304622 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 44
Match NameE-valueIdentityDescription
PIP21_MAIZE2.581e-4987.30Aquaporin PIP2-1 OS=Zea mays GN=PIP2-1 PE=1 SV=2[more]
PIP25_ARATH3.366e-4987.30Probable aquaporin PIP2-5 OS=Arabidopsis thaliana ... [more]
PIP22_MAIZE5.716e-4985.71Aquaporin PIP2-2 OS=Zea mays GN=PIP2-2 PE=2 SV=1[more]
PIP26_ARATH2.803e-4885.71Probable aquaporin PIP2-6 OS=Arabidopsis thaliana ... [more]
PIP21_ARATH4.762e-4884.13Aquaporin PIP2-1 OS=Arabidopsis thaliana GN=PIP2-1... [more]
PIP24_ARATH6.205e-4885.71Probable aquaporin PIP2-4 OS=Arabidopsis thaliana ... [more]
PIP21_ORYSJ1.054e-4780.95Probable aquaporin PIP2-1 OS=Oryza sativa subsp. j... [more]
PIP22_ARATH1.054e-4784.13Aquaporin PIP2-2 OS=Arabidopsis thaliana GN=PIP2-2... [more]
PIP24_MAIZE1.791e-4786.67Aquaporin PIP2-4 OS=Zea mays GN=PIP2-4 PE=1 SV=1[more]
PIP23_MAIZE1.791e-4786.67Aquaporin PIP2-3 OS=Zea mays GN=PIP2-3 PE=2 SV=1[more]

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Properties
Property NameValue
Genbank descriptionFLAVEDO0001_IaR_E10 Flavedo Mature Citrus sinensis cDNA clone FLAVEDO0001_IaR_E10 3, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB304622 ID=CB304622; Name=CB304622; organism=Citrus sinensis; type=EST; length=608bp
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAAGTGCTTGAAAACAAC
ACAGGGGATTTACAAAAAAGCCCCTCATTGTCTAAGAGACTTTTTGCCTA
CAACTCCTTGAAAGCACAAATCACACCCCCCTCATTATTCACTTTTTGAA
ACTTAACACACCTCTACTCCTTCCCAGTTTTTTCTTTCCAATTTTAAATC
ATTAACCCCATGAAGATGTGGATAATCTACTTGTCGAAGAAACTAAAATC
AGACACGGGATTGGCTCCTGAATGAACCAAGAGCCTTGACAGCTCCAGCT
CTCAAGATGAATTGGTGGTAGAAAGCTGCAATTGCTGCACCAATGAAGGG
TCCAACCCAGAAGAGCCATTGATCATCCCAAACCTTGTCCTTGTTGTAAA
TAACAGCGGCCCCGAGACTCCTTGCTGGGTTAATGCCAGTGCCAGTGATT
GGGATTGTGGCCAGTGTACCATGAACACAGCAAATCCAATGGGGAGCGGT
GCCAAAACGGGAACGTGGGAGTCTCTGGCGCTTCTCTTGGGATCAGTGGC
CGAGAAGACAGTGTAAACCAAGACAAAGGTGCCGATGATCTCGGCGGCCA
ATCCAACG
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