CF417249

Overview
NameCF417249
Unique NameCF417249
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length700
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF417249 vs. ExPASy Swiss-Prot
Match: MDH_ERWT9 (Malate dehydrogenase OS=Erwinia tasmaniensis (strain DSM 17950 / Et1/99) GN=mdh PE=3 SV=1)

HSP 1 Score: 172.555 bits (436), Expect = 5.354e-47
Identity = 90/157 (57.32%), Postives = 110/157 (70.06%), Query Frame = 1 
Query:  115 KVAILGAAGGIGQPLAMLMKIN-PLVSVLHLYDVVN-TPGVTADISHMDTGAVVRGFLGQPQLENALTGVDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLCEGIAKCCPNATVNLISNPVNSTVPIAAEVFKKAGTYDPKKLLGVTMLDVVRANTF 579
            KV +LGAAGGIGQ LA+L+K   P  S L LYD+   TPGV  D+SH+ T   + GF G+     AL G D+V+I AGV RKPGM R DLFN+NAGIVR L E +A  CP A + +I+NPVN+TV IAAEV KKAG YD  +L G+T LD++RAN F
Sbjct:    2 KVTVLGAAGGIGQALALLLKTQLPAGSELSLYDIAPVTPGVAVDLSHIPTSVKIAGFSGEDATP-ALKGADVVLISAGVARKPGMDRSDLFNVNAGIVRNLIEQVAATCPKALIGIITNPVNTTVAIAAEVLKKAGVYDKNRLFGITTLDIIRANAF 157

HSP 2 Score: 36.5798 bits (83), Expect = 5.354e-47
Identity = 23/39 (58.97%), Postives = 27/39 (69.23%), Query Frame = 2 
Query:  581 VAEVLGLDP*GMLMFLVVGGHAGVTILPLLSQVKPPCSF 697
            VAE+ G  P   +   V+GGH+GVTILPLLSQV P  SF
Sbjct:  158 VAELKGKQP-EEVNVPVIGGHSGVTILPLLSQV-PGVSF 194
[Close]
BLAST of CF417249 vs. ExPASy Swiss-Prot
Match: MDH_SHEHH (Malate dehydrogenase OS=Shewanella halifaxensis (strain HAW-EB4) GN=mdh PE=3 SV=1)

HSP 1 Score: 172.555 bits (436), Expect = 1.185e-46
Identity = 97/173 (56.07%), Postives = 117/173 (67.63%), Query Frame = 1 
Query:  115 KVAILGAAGGIGQPLAMLMKIN-PLVSVLHLYDVVNT-PGVTADISHMDTGAVVRGFLGQPQLENALTGVDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLCEGIAKCCPNATVNLISNPVNSTVPIAAEVFKKAGTYDPKKLLGVTMLDVVRANTFRGGSFGT*SLGDVDVP 627
            KVA+LGAAGGIGQ LA+L+K   P  S L LYD+    PGV  D+SH+ T   V+GF G+     AL G D+V+I AGV RKPGM R DLFNINAGIVR L E  A  CP A + +I+NPVN+TV IAAEV KKAG YD  +L GVT LDV+R+ TF   +    ++ DV VP
Sbjct:    2 KVAVLGAAGGIGQALALLLKTQLPAGSKLSLYDIAPVIPGVAVDLSHIPTAVEVKGFAGEDPTA-ALEGADVVLISAGVARKPGMDRSDLFNINAGIVRNLVEKCAATCPKALIGIITNPVNTTVAIAAEVLKKAGVYDKNRLFGVTTLDVIRSETFVAEAKDL-NVADVKVP 172

HSP 2 Score: 35.4242 bits (80), Expect = 1.185e-46
Identity = 15/18 (83.33%), Postives = 18/18 (100.00%), Query Frame = 2 
Query:  629 VVGGHAGVTILPLLSQVK 682
            V+GGH+GVTILPLLSQV+
Sbjct:  173 VIGGHSGVTILPLLSQVE 190
[Close]
BLAST of CF417249 vs. ExPASy Swiss-Prot
Match: MDH_SHESH (Malate dehydrogenase OS=Shewanella sediminis (strain HAW-EB3) GN=mdh PE=3 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 1.545e-46
Identity = 93/157 (59.24%), Postives = 110/157 (70.06%), Query Frame = 1 
Query:  115 KVAILGAAGGIGQPLAMLMKIN-PLVSVLHLYDVVN-TPGVTADISHMDTGAVVRGFLGQPQLENALTGVDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLCEGIAKCCPNATVNLISNPVNSTVPIAAEVFKKAGTYDPKKLLGVTMLDVVRANTF 579
            KVA+LGAAGGIGQ LA+L+K   P  S L LYD+   TPGV  D+SH+ T   V+GF G+     AL G D+V+I AGV RKPGM R DLFNINAGIVR L E  A  CP A + +I+NPVN+TV IAAEV K AG YD  +L GVT LDV+R+ TF
Sbjct:    2 KVAVLGAAGGIGQALALLLKTQLPAGSKLSLYDIAPVTPGVAVDLSHIPTAVEVKGFAGEDPTP-ALEGADVVLISAGVARKPGMDRSDLFNINAGIVRNLVEKCAAACPKALIGIITNPVNTTVAIAAEVLKAAGVYDKNRLFGVTTLDVIRSETF 157

HSP 2 Score: 35.4242 bits (80), Expect = 1.545e-46
Identity = 15/18 (83.33%), Postives = 18/18 (100.00%), Query Frame = 2 
Query:  629 VVGGHAGVTILPLLSQVK 682
            V+GGH+GVTILPLLSQV+
Sbjct:  173 VIGGHSGVTILPLLSQVQ 190
[Close]
BLAST of CF417249 vs. ExPASy Swiss-Prot
Match: MDH_PSEA6 (Malate dehydrogenase OS=Pseudoalteromonas atlantica (strain T6c / BAA-1087) GN=mdh PE=3 SV=1)

HSP 1 Score: 173.326 bits (438), Expect = 2.013e-46
Identity = 94/159 (59.12%), Postives = 112/159 (70.44%), Query Frame = 1 
Query:  115 KVAILGAAGGIGQPLAMLMKIN-PLVSVLHLYDVVNT-PGVTADISHMDTGAVVRGFLGQPQLENALTGVDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLCEGIAKCCPNATVNLISNPVNSTVPIAAEVFKKAGTYDPKKLLGVTMLDVVRANTFRG 585
            KVA+LGAAGGIGQ L++L+K   P  + L LYDV    PGV  D+SH+ T   V GF G+  L  ALTG D+V+IPAGVPRKPGM R DLFNINAGIVR L + +A  CP A + +I+NPVN+TV IAAE  K  G Y+  KL GVT LDV+RA TF G
Sbjct:    2 KVAVLGAAGGIGQALSLLLKTQLPAGTELALYDVAPVVPGVAVDLSHIPTDVKVEGF-GKDDLAKALTGSDIVLIPAGVPRKPGMDRSDLFNINAGIVRNLVDSVADNCPEACLCIITNPVNTTVAIAAEALKAKGVYNKNKLFGVTTLDVIRAETFVG 159

HSP 2 Score: 33.8834 bits (76), Expect = 2.013e-46
Identity = 14/18 (77.78%), Postives = 17/18 (94.44%), Query Frame = 2 
Query:  629 VVGGHAGVTILPLLSQVK 682
            V+GGH+G TILPLLSQV+
Sbjct:  173 VIGGHSGTTILPLLSQVE 190
[Close]
BLAST of CF417249 vs. ExPASy Swiss-Prot
Match: MDH_AERS4 (Malate dehydrogenase OS=Aeromonas salmonicida (strain A449) GN=mdh PE=3 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 2.013e-46
Identity = 93/157 (59.24%), Postives = 111/157 (70.70%), Query Frame = 1 
Query:  115 KVAILGAAGGIGQPLAMLMKIN-PLVSVLHLYDVVN-TPGVTADISHMDTGAVVRGFLGQPQLENALTGVDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLCEGIAKCCPNATVNLISNPVNSTVPIAAEVFKKAGTYDPKKLLGVTMLDVVRANTF 579
            KVA+LGAAGGIGQ LA+L+K   P  S L LYD+   TPGV  D+SH+ T   V+GF G+     AL G D+V+I AGV RKPGM R DLFNIN GIV+ L E  A  CP A + +I+NPVN+TV IAAEV KKAG YD ++L GVT LDV+RA TF
Sbjct:    2 KVAVLGAAGGIGQALALLLKNRLPAGSELSLYDIAPVTPGVAVDLSHIPTDVKVKGFCGEDP-SPALVGADVVLISAGVARKPGMDRSDLFNINPGIVKNLVEKCAASCPKALIGIITNPVNTTVAIAAEVLKKAGVYDKRRLFGVTTLDVIRAETF 157

HSP 2 Score: 35.039 bits (79), Expect = 2.013e-46
Identity = 14/18 (77.78%), Postives = 18/18 (100.00%), Query Frame = 2 
Query:  629 VVGGHAGVTILPLLSQVK 682
            V+GGH+GVTILPLLSQ++
Sbjct:  173 VIGGHSGVTILPLLSQIE 190
[Close]
BLAST of CF417249 vs. ExPASy Swiss-Prot
Match: MDH_SHEPW (Malate dehydrogenase OS=Shewanella piezotolerans (strain WP3 / JCM 13877) GN=mdh PE=3 SV=1)

HSP 1 Score: 171.014 bits (432), Expect = 4.456e-46
Identity = 97/172 (56.40%), Postives = 117/172 (68.02%), Query Frame = 1 
Query:  115 KVAILGAAGGIGQPLAMLMKIN-PLVSVLHLYDVVN-TPGVTADISHMDTGAVVRGFLGQPQLENALTGVDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLCEGIAKCCPNATVNLISNPVNSTVPIAAEVFKKAGTYDPKKLLGVTMLDVVRANTFRGGSFGT*SLGDVDV 624
            KVA+LGAAGGIGQ LA+L+K   P  S L LYD+   TPGV  D+SH+ T   V+GF G+     AL G D+V+I AGV RKPGM R DLFNINAGIVR L E  A   P A + +I+NPVN+TV IAAEV KKAG YD  +L GVT LDV+R+ TF   + G  ++ DV V
Sbjct:    2 KVAVLGAAGGIGQALALLLKTQLPAGSKLSLYDIAPVTPGVAVDLSHIPTDVEVKGFAGEDPTA-ALEGADVVLISAGVARKPGMDRSDLFNINAGIVRNLVEKCAATSPKALIGIITNPVNTTVAIAAEVLKKAGVYDKNRLFGVTTLDVIRSETFVAAAKGL-NVADVKV 171

HSP 2 Score: 35.039 bits (79), Expect = 4.456e-46
Identity = 14/18 (77.78%), Postives = 18/18 (100.00%), Query Frame = 2 
Query:  629 VVGGHAGVTILPLLSQVK 682
            V+GGH+GVTILPLLSQ++
Sbjct:  173 VIGGHSGVTILPLLSQIE 190
[Close]
BLAST of CF417249 vs. ExPASy Swiss-Prot
Match: MDH_ACTPJ (Malate dehydrogenase OS=Actinobacillus pleuropneumoniae serotype 3 (strain JL03) GN=mdh PE=3 SV=1)

HSP 1 Score: 172.94 bits (437), Expect = 5.806e-46
Identity = 93/157 (59.24%), Postives = 115/157 (73.25%), Query Frame = 1 
Query:  115 KVAILGAAGGIGQPLAMLMKIN-PLVSVLHLYDVVN-TPGVTADISHMDTGAVVRGFLGQPQLENALTGVDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLCEGIAKCCPNATVNLISNPVNSTVPIAAEVFKKAGTYDPKKLLGVTMLDVVRANTF 579
            KVA+LGAAGGIGQ LA+L+K+  P+ + L LYD+   TPG+  DISH+ T     G+ G+   E AL G +LVII AGV RKPGMTR DLFNINA IV+ L E +A+ CP A + +++NPVN+ VPIAAEV +KAG YD +KL GVT LDVVRA TF
Sbjct:    2 KVALLGAAGGIGQTLALLLKLRLPVGTDLALYDISPVTPGIAVDISHIPTSVSAVGYSGEDPSE-ALKGANLVIITAGVARKPGMTRADLFNINADIVKNLVEKVAEVCPKACIGIVTNPVNTLVPIAAEVLRKAGVYDKRKLFGVTTLDVVRAKTF 157

HSP 2 Score: 32.7278 bits (73), Expect = 5.806e-46
Identity = 17/27 (62.96%), Postives = 19/27 (70.37%), Query Frame = 2 
Query:  629 VVGGHAGVTILPLLSQVKP---PCSFT 700
            V+GGH+G TILPLLSQ      P SFT
Sbjct:  173 VIGGHSGPTILPLLSQALSEGLPLSFT 199
[Close]
BLAST of CF417249 vs. ExPASy Swiss-Prot
Match: MDH_ACTP7 (Malate dehydrogenase OS=Actinobacillus pleuropneumoniae serotype 7 (strain AP76) GN=mdh PE=3 SV=1)

HSP 1 Score: 172.94 bits (437), Expect = 5.806e-46
Identity = 93/157 (59.24%), Postives = 115/157 (73.25%), Query Frame = 1 
Query:  115 KVAILGAAGGIGQPLAMLMKIN-PLVSVLHLYDVVN-TPGVTADISHMDTGAVVRGFLGQPQLENALTGVDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLCEGIAKCCPNATVNLISNPVNSTVPIAAEVFKKAGTYDPKKLLGVTMLDVVRANTF 579
            KVA+LGAAGGIGQ LA+L+K+  P+ + L LYD+   TPG+  DISH+ T     G+ G+   E AL G +LVII AGV RKPGMTR DLFNINA IV+ L E +A+ CP A + +++NPVN+ VPIAAEV +KAG YD +KL GVT LDVVRA TF
Sbjct:    2 KVALLGAAGGIGQTLALLLKLRLPVGTDLALYDISPVTPGIAVDISHIPTSVSAVGYSGEDPSE-ALKGANLVIITAGVARKPGMTRADLFNINADIVKNLVEKVAEVCPKACIGIVTNPVNTLVPIAAEVLRKAGVYDKRKLFGVTTLDVVRAKTF 157

HSP 2 Score: 32.7278 bits (73), Expect = 5.806e-46
Identity = 17/27 (62.96%), Postives = 19/27 (70.37%), Query Frame = 2 
Query:  629 VVGGHAGVTILPLLSQVKP---PCSFT 700
            V+GGH+G TILPLLSQ      P SFT
Sbjct:  173 VIGGHSGPTILPLLSQALSEGLPLSFT 199
[Close]
BLAST of CF417249 vs. ExPASy Swiss-Prot
Match: MDH_ACTP2 (Malate dehydrogenase OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) GN=mdh PE=3 SV=1)

HSP 1 Score: 172.94 bits (437), Expect = 5.806e-46
Identity = 93/157 (59.24%), Postives = 115/157 (73.25%), Query Frame = 1 
Query:  115 KVAILGAAGGIGQPLAMLMKIN-PLVSVLHLYDVVN-TPGVTADISHMDTGAVVRGFLGQPQLENALTGVDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLCEGIAKCCPNATVNLISNPVNSTVPIAAEVFKKAGTYDPKKLLGVTMLDVVRANTF 579
            KVA+LGAAGGIGQ LA+L+K+  P+ + L LYD+   TPG+  DISH+ T     G+ G+   E AL G +LVII AGV RKPGMTR DLFNINA IV+ L E +A+ CP A + +++NPVN+ VPIAAEV +KAG YD +KL GVT LDVVRA TF
Sbjct:    2 KVALLGAAGGIGQTLALLLKLRLPVGTDLALYDISPVTPGIAVDISHIPTSVSAVGYSGEDPSE-ALKGANLVIITAGVARKPGMTRADLFNINADIVKNLVEKVAEVCPKACIGIVTNPVNTLVPIAAEVLRKAGVYDKRKLFGVTTLDVVRAKTF 157

HSP 2 Score: 32.7278 bits (73), Expect = 5.806e-46
Identity = 17/27 (62.96%), Postives = 19/27 (70.37%), Query Frame = 2 
Query:  629 VVGGHAGVTILPLLSQVKP---PCSFT 700
            V+GGH+G TILPLLSQ      P SFT
Sbjct:  173 VIGGHSGPTILPLLSQALSEGLPLSFT 199
[Close]
BLAST of CF417249 vs. ExPASy Swiss-Prot
Match: MDH_VIBMI (Malate dehydrogenase (Fragment) OS=Vibrio mimicus GN=mdh PE=3 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 9.912e-46
Identity = 89/151 (58.94%), Postives = 109/151 (72.19%), Query Frame = 1 
Query:  133 AAGGIGQPLAMLMKIN-PLVSVLHLYDVVN-TPGVTADISHMDTGAVVRGFLGQPQLENALTGVDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLCEGIAKCCPNATVNLISNPVNSTVPIAAEVFKKAGTYDPKKLLGVTMLDVVRANTF 579
            AAGGIGQ LA+L+K   P  S L LYD+   TPGV AD+SH+ T   ++G+ G+     AL G D+V++ AGV RKPGM R DLFN+NAGIV+ L E IA  CP A V +I+NPVN+TVPIAAEV KKAG YD +KL GVT LDV+R+ TF
Sbjct:    1 AAGGIGQALALLLKNRLPAGSDLALYDIAPVTPGVAADLSHIPTPVTIKGYAGEDPTP-ALEGADVVLVSAGVARKPGMDRADLFNVNAGIVKALAETIAVVCPKACVGIITNPVNTTVPIAAEVLKKAGVYDKRKLFGVTTLDVIRSETF 150

HSP 2 Score: 37.7354 bits (86), Expect = 9.912e-46
Identity = 20/34 (58.82%), Postives = 25/34 (73.53%), Query Frame = 2 
Query:  581 VAEVLGLDP*GMLMFLVVGGHAGVTILPLLSQVK 682
            VA +   DP G +   V+GGH+GVTILPLLSQV+
Sbjct:  151 VAALKDKDP-GQVRVPVIGGHSGVTILPLLSQVE 183
The following BLAST results are available for this feature:
BLAST of CF417249 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 293
Match NameE-valueIdentityDescription
MDH_ERWT95.354e-4757.32Malate dehydrogenase OS=Erwinia tasmaniensis (stra... [more]
MDH_SHEHH1.185e-4656.07Malate dehydrogenase OS=Shewanella halifaxensis (s... [more]
MDH_SHESH1.545e-4659.24Malate dehydrogenase OS=Shewanella sediminis (stra... [more]
MDH_PSEA62.013e-4659.12Malate dehydrogenase OS=Pseudoalteromonas atlantic... [more]
MDH_AERS42.013e-4659.24Malate dehydrogenase OS=Aeromonas salmonicida (str... [more]
MDH_SHEPW4.456e-4656.40Malate dehydrogenase OS=Shewanella piezotolerans (... [more]
MDH_ACTPJ5.806e-4659.24Malate dehydrogenase OS=Actinobacillus pleuropneum... [more]
MDH_ACTP75.806e-4659.24Malate dehydrogenase OS=Actinobacillus pleuropneum... [more]
MDH_ACTP25.806e-4659.24Malate dehydrogenase OS=Actinobacillus pleuropneum... [more]
MDH_VIBMI9.912e-4658.94Malate dehydrogenase (Fragment) OS=Vibrio mimicus ... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_115000-179 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-003_B05 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF417249 ID=CF417249; Name=CF417249; organism=Citrus sinensis; type=EST; length=700bp
AATCAGCGCATTGCTAGAATCTCTGCTCATCTTTACCCTCCAAATCTCCA
GATGGAGGAGAATTCTTGCTTGAGGCAAGCTAAATGCCGGGCAAAAGGCG
GGGCAGCTGGATTCAAAGTAGCCATCTTGGGTGCTGCTGGAGGCATTGGT
CAACCCCTTGCAATGCTAATGAAGATAAACCCCCTGGTCTCAGTCCTTCA
CCTCTATGATGTTGTCAATACCCCTGGTGTCACTGCTGATATTAGCCATA
TGGACACCGGCGCTGTGGTACGGGGTTTCCTAGGCCAGCCACAGCTTGAG
AATGCTCTCACAGGGGTGGACCTTGTCATTATCCCTGCCGGCGTTCCGAG
GAAGCCTGGAATGACCAGGGATGATCTTTTCAATATCAATGCTGGAATTG
TCAGGACACTCTGTGAAGGCATCGCAAAGTGTTGCCCCAATGCGACTGTC
AATTTGATCAGTAATCCAGTGAACTCTACAGTTCCTATTGCCGCAGAGGT
TTTCAAGAAAGCTGGTACTTATGATCCAAAGAAACTCTTGGGAGTTACAA
TGCTCGATGTCGTAAGAGCCAACACATTCCGTGGCGGAAGTTTTGGGACT
TGATCCCTAGGGGATGTTGATGTTCCTGGTTGTTGGAGGTCATGCTGGAG
TAACAATTTTGCCACTTTTATCACAGGTTAAGCCTCCTTGTAGCTTCACT
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