CF417249

Overview
NameCF417249
Unique NameCF417249
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length700
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF417249 vs. ExPASy Swiss-Prot
Match: MDHG2_ARATH (Probable malate dehydrogenase, glyoxysomal OS=Arabidopsis thaliana GN=At2g22780 PE=1 SV=1)

HSP 1 Score: 331.643 bits (849), Expect = 2.575e-101
Identity = 168/195 (86.15%), Postives = 177/195 (90.77%), Query Frame = 1
Query:    1 NQRIARISAHLYPPNL--QMEENSCLRQAKCRAKGGAAGFKVAILGAAGGIGQPLAMLMKINPLVSVLHLYDVVNTPGVTADISHMDTGAVVRGFLGQPQLENALTGVDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLCEGIAKCCPNATVNLISNPVNSTVPIAAEVFKKAGTYDPKKLLGVTMLDVVRANTF 579
            NQRIARISAHL PPNL  Q+ + S L +  CRAKGG+ GFKVAILGAAGGIGQPLAMLMK+NPLVSVLHLYDV N PGVTADISHMDT AVVRGFLGQPQLE ALTG+DLVIIPAGVPRKPGMTRDDLFNINAGIVRTL E IAKCCP A VN+ISNPVNSTVPIAAEVFKKAGT+DPKKL+GVTMLDVVRANTF
Sbjct:    4 NQRIARISAHLNPPNLHNQIADGSGLNRVACRAKGGSPGFKVAILGAAGGIGQPLAMLMKMNPLVSVLHLYDVANAPGVTADISHMDTSAVVRGFLGQPQLEEALTGMDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLSEAIAKCCPKAIVNIISNPVNSTVPIAAEVFKKAGTFDPKKLMGVTMLDVVRANTF 198          

HSP 2 Score: 58.9214 bits (141), Expect = 2.575e-101
Identity = 31/40 (77.50%), Postives = 33/40 (82.50%), Query Frame = 2
Query:  581 VAEVLGLDP*GMLMFLVVGGHAGVTILPLLSQVKPPCSFT 700
            VAEV+ LDP   +   VVGGHAGVTILPLLSQVKPPCSFT
Sbjct:  199 VAEVMSLDP-REVEVPVVGGHAGVTILPLLSQVKPPCSFT 237          
BLAST of CF417249 vs. ExPASy Swiss-Prot
Match: MDHG_CITLA (Malate dehydrogenase, glyoxysomal OS=Citrullus lanatus PE=1 SV=1)

HSP 1 Score: 352.058 bits (902), Expect = 1.717e-96
Identity = 179/209 (85.65%), Postives = 188/209 (89.95%), Query Frame = 1
Query:    1 NQRIARISAHLYPPNLQMEENSCLRQAKCRAKGGAAGFKVAILGAAGGIGQPLAMLMKINPLVSVLHLYDVVNTPGVTADISHMDTGAVVRGFLGQPQLENALTGVDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLCEGIAKCCPNATVNLISNPVNSTVPIAAEVFKKAGTYDPKKLLGVTMLDVVRANTFRGGSFGT*SLGDVDVP 627
            NQRIARISAHL+PP  QMEE+S LR+A CRAKGGA GFKVAILGAAGGIGQPLAMLMK+NPLVSVLHLYDVVN PGVTADISHMDTGAVVRGFLGQ QLE ALTG+DL+I+PAGVPRKPGMTRDDLF INAGIV+TLCEGIAKCCP A VNLISNPVNSTVPIAAEVFKKAGTYDPK+LLGVTMLDVVRANTF     G     DVDVP
Sbjct:    8 NQRIARISAHLHPPKSQMEESSALRRANCRAKGGAPGFKVAILGAAGGIGQPLAMLMKMNPLVSVLHLYDVVNAPGVTADISHMDTGAVVRGFLGQQQLEAALTGMDLIIVPAGVPRKPGMTRDDLFKINAGIVKTLCEGIAKCCPRAIVNLISNPVNSTVPIAAEVFKKAGTYDPKRLLGVTMLDVVRANTFVAEVLGL-DPRDVDVP 215          
BLAST of CF417249 vs. ExPASy Swiss-Prot
Match: MDHG_CUCSA (Malate dehydrogenase, glyoxysomal OS=Cucumis sativus GN=MDHG PE=2 SV=1)

HSP 1 Score: 351.288 bits (900), Expect = 2.928e-96
Identity = 179/209 (85.65%), Postives = 188/209 (89.95%), Query Frame = 1
Query:    1 NQRIARISAHLYPPNLQMEENSCLRQAKCRAKGGAAGFKVAILGAAGGIGQPLAMLMKINPLVSVLHLYDVVNTPGVTADISHMDTGAVVRGFLGQPQLENALTGVDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLCEGIAKCCPNATVNLISNPVNSTVPIAAEVFKKAGTYDPKKLLGVTMLDVVRANTFRGGSFGT*SLGDVDVP 627
            NQRIARISAHL+PP  QMEE+S LR+A CRAKGGA GFKVAILGAAGGIGQPLAMLMK+NPLVSVLHLYDVVN PGVTADISHMDTGAVVRGFLGQ QLE ALTG+DLV+IPAGVPRKPGMTRDDLF INAGIV+TLCEGIAKCCP A VNLISNPVNSTVPIAAEVFKKAGTYDPK+LLGVTMLDVVRANTF     G     DV+VP
Sbjct:    8 NQRIARISAHLHPPKYQMEESSVLRRANCRAKGGAPGFKVAILGAAGGIGQPLAMLMKMNPLVSVLHLYDVVNAPGVTADISHMDTGAVVRGFLGQQQLERALTGMDLVVIPAGVPRKPGMTRDDLFKINAGIVKTLCEGIAKCCPTAIVNLISNPVNSTVPIAAEVFKKAGTYDPKRLLGVTMLDVVRANTFVAEVLGL-DPRDVNVP 215          
BLAST of CF417249 vs. ExPASy Swiss-Prot
Match: MDHG_ORYSJ (Malate dehydrogenase, glyoxysomal OS=Oryza sativa subsp. japonica GN=Os12g0632700 PE=1 SV=3)

HSP 1 Score: 337.806 bits (865), Expect = 3.351e-92
Identity = 171/208 (82.21%), Postives = 186/208 (89.42%), Query Frame = 1
Query:    4 QRIARISAHLYPPNLQMEENSCLRQAKCRAKGGAAGFKVAILGAAGGIGQPLAMLMKINPLVSVLHLYDVVNTPGVTADISHMDTGAVVRGFLGQPQLENALTGVDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLCEGIAKCCPNATVNLISNPVNSTVPIAAEVFKKAGTYDPKKLLGVTMLDVVRANTFRGGSFGT*SLGDVDVP 627
            +R+ R+++HL PP  QMEE+  LR + CRAKG A GFKVAILGA+GGIGQPLA+LMK+NPLVSVLHLYDVVNTPGVTADISHM+TGAVVRGFLGQPQLENALTG+DLVIIPAGVPRKPGMTRDDLFNINAGIVRTLCEGIAKCCPNA VN+ISNPVNSTVPIAAEVFKKAGTYDPK+LLGVT LDVVRANTF     G     DV+VP
Sbjct:    9 RRMERLASHLRPPASQMEESPLLRGSNCRAKGAAPGFKVAILGASGGIGQPLALLMKMNPLVSVLHLYDVVNTPGVTADISHMNTGAVVRGFLGQPQLENALTGMDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLCEGIAKCCPNAIVNVISNPVNSTVPIAAEVFKKAGTYDPKRLLGVTTLDVVRANTFVAEVLGL-DPRDVNVP 215          
BLAST of CF417249 vs. ExPASy Swiss-Prot
Match: MDHG_SOYBN (Malate dehydrogenase, glyoxysomal OS=Glycine max PE=2 SV=2)

HSP 1 Score: 327.02 bits (837), Expect = 5.914e-89
Identity = 166/209 (79.43%), Postives = 184/209 (88.04%), Query Frame = 1
Query:    1 NQRIARISAHLYPPNLQMEENSCLRQAKCRAKGGAAGFKVAILGAAGGIGQPLAMLMKINPLVSVLHLYDVVNTPGVTADISHMDTGAVVRGFLGQPQLENALTGVDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLCEGIAKCCPNATVNLISNPVNSTVPIAAEVFKKAGTYDPKKLLGVTMLDVVRANTFRGGSFGT*SLGDVDVP 627
            + RI+RI+ HL P   Q E++ CL+++ CRAKGG +GFKVAILGAAGGIGQPLAMLMK+NPLVS+LHLYDVVNTPGVT+DISHMDTGAVVRGFLGQ QLE+AL G+DLVIIPAGVPRKPGMTRDDLFNINAGIV+TLCE IAKCCP A VN+ISNPVNSTVPIAAEVFK+AGTYDPK+LLGVTMLDVVRANTF     G     DVDVP
Sbjct:    8 SDRISRIAGHLRP---QREDDVCLKRSDCRAKGGVSGFKVAILGAAGGIGQPLAMLMKMNPLVSLLHLYDVVNTPGVTSDISHMDTGAVVRGFLGQQQLEDALIGMDLVIIPAGVPRKPGMTRDDLFNINAGIVKTLCEAIAKCCPKAIVNVISNPVNSTVPIAAEVFKRAGTYDPKRLLGVTMLDVVRANTFVAEVLGV-DPRDVDVP 212          
BLAST of CF417249 vs. ExPASy Swiss-Prot
Match: MDHG1_ARATH (Malate dehydrogenase, glyoxysomal OS=Arabidopsis thaliana GN=At5g09660 PE=1 SV=1)

HSP 1 Score: 320.857 bits (821), Expect = 4.238e-87
Identity = 167/210 (79.52%), Postives = 181/210 (86.19%), Query Frame = 1
Query:    1 NQRIARISAHLYPPNLQME-ENSCLRQAKCRAKGGAAGFKVAILGAAGGIGQPLAMLMKINPLVSVLHLYDVVNTPGVTADISHMDTGAVVRGFLGQPQLENALTGVDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLCEGIAKCCPNATVNLISNPVNSTVPIAAEVFKKAGTYDPKKLLGVTMLDVVRANTFRGGSFGT*SLGDVDVP 627
            NQRIARISAHL P   QME +NS + +  CRAKGG  GFKVAILGAAGGIGQ L++LMK+NPLVS+LHLYDVVN PGVTAD+SHMDTGAVVRGFLG  QLE+ALTG+DLVIIPAG+PRKPGMTRDDLF INAGIV+TLCEG+AKCCPNA VNLISNPVNSTVPIAAEVFKKAGTYDPKKLLGVT LDV RANTF     G     +VDVP
Sbjct:    8 NQRIARISAHLTP---QMEAKNSVIGRENCRAKGGNPGFKVAILGAAGGIGQSLSLLMKMNPLVSLLHLYDVVNAPGVTADVSHMDTGAVVRGFLGAKQLEDALTGMDLVIIPAGIPRKPGMTRDDLFKINAGIVKTLCEGVAKCCPNAIVNLISNPVNSTVPIAAEVFKKAGTYDPKKLLGVTTLDVARANTFVAEVLGL-DPREVDVP 213          
BLAST of CF417249 vs. ExPASy Swiss-Prot
Match: MDHG2_BRANA (Malate dehydrogenase 2, glyoxysomal OS=Brassica napus GN=MDH2 PE=3 SV=1)

HSP 1 Score: 313.923 bits (803), Expect = 5.181e-85
Identity = 165/211 (78.20%), Postives = 179/211 (84.83%), Query Frame = 1
Query:    1 NQRIARISAHLYPP-NLQME-ENSCLRQAKCRAKGGAAGFKVAILGAAGGIGQPLAMLMKINPLVSVLHLYDVVNTPGVTADISHMDTGAVVRGFLGQPQLENALTGVDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLCEGIAKCCPNATVNLISNPVNSTVPIAAEVFKKAGTYDPKKLLGVTMLDVVRANTFRGGSFGT*SLGDVDVP 627
            N+RIA ISAHL P    QME +NS + +  CRAKGG  GFKVAILGAAGGIGQ L++LMK+NPLVS+LHLYDVVN PGVTAD+SHMDTGAVVRGFLG  QLE+ALTG+DLVIIPAGVPRKPGMTRDDLF INAGIV+TLCEG+AKCCPNA VNLISNPVNSTV IAAEVFKKAGTYDPKKLLGVT LDV RANTF     G     +VDVP
Sbjct:    8 NKRIAMISAHLQPSFTPQMEAKNSVMGRENCRAKGGNPGFKVAILGAAGGIGQSLSLLMKMNPLVSLLHLYDVVNAPGVTADVSHMDTGAVVRGFLGAKQLEDALTGMDLVIIPAGVPRKPGMTRDDLFKINAGIVKTLCEGVAKCCPNAIVNLISNPVNSTVAIAAEVFKKAGTYDPKKLLGVTTLDVARANTFVAEVLGL-DPREVDVP 217          
BLAST of CF417249 vs. ExPASy Swiss-Prot
Match: MDHG1_BRANA (Malate dehydrogenase 1, glyoxysomal OS=Brassica napus GN=MDH1 PE=2 SV=2)

HSP 1 Score: 311.612 bits (797), Expect = 2.571e-84
Identity = 165/210 (78.57%), Postives = 177/210 (84.29%), Query Frame = 1
Query:    4 QRIARISAHLYPP-NLQME-ENSCLRQAKCRAKGGAAGFKVAILGAAGGIGQPLAMLMKINPLVSVLHLYDVVNTPGVTADISHMDTGAVVRGFLGQPQLENALTGVDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLCEGIAKCCPNATVNLISNPVNSTVPIAAEVFKKAGTYDPKKLLGVTMLDVVRANTFRGGSFGT*SLGDVDVP 627
            +RIA ISAHL P    QME +NS +    CRAKGG  GFKVAILGAAGGIGQ L++LMK+NPLVS+LHLYDVVN PGVTAD+SHMDTGAVVRGFLG  QLE+ALTG+DLVIIPAGVPRKPGMTRDDLF INAGIVRTLCEG+AKCCPNA VNLISNPVNSTV IAAEVFKKAGTYDPKKLLGVT LDV RANTF     G     +VDVP
Sbjct:    9 KRIAMISAHLQPSFTPQMEAKNSVMGLESCRAKGGNPGFKVAILGAAGGIGQSLSLLMKMNPLVSLLHLYDVVNAPGVTADVSHMDTGAVVRGFLGAKQLEDALTGMDLVIIPAGVPRKPGMTRDDLFKINAGIVRTLCEGVAKCCPNAIVNLISNPVNSTVAIAAEVFKKAGTYDPKKLLGVTTLDVARANTFVAEVLGL-DPREVDVP 217          
BLAST of CF417249 vs. ExPASy Swiss-Prot
Match: MDHM_CITLA (Malate dehydrogenase, mitochondrial OS=Citrullus lanatus GN=MMDH PE=1 SV=1)

HSP 1 Score: 241.891 bits (616), Expect = 2.858e-67
Identity = 121/171 (70.76%), Postives = 140/171 (81.87%), Query Frame = 1
Query:  115 KVAILGAAGGIGQPLAMLMKINPLVSVLHLYDVVNTPGVTADISHMDTGAVVRGFLGQPQLENALTGVDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLCEGIAKCCPNATVNLISNPVNSTVPIAAEVFKKAGTYDPKKLLGVTMLDVVRANTFRGGSFGT*SLGDVDVP 627
            KVA+LGAAGGIGQPLA+LMK+NPLVS L LYD+  TPGV AD+ H++T + V G++G+ QL  AL G D+VIIPAGVPRKPGMTRDDLFNINAGIV++LC  IAK CPNA +N+ISNPVNSTVPIAAEVFKKAGTYD KKL GVT LDVVRA TF  G      + +V+VP
Sbjct:   36 KVAVLGAAGGIGQPLALLMKLNPLVSKLALYDIAGTPGVAADVGHVNTRSEVTGYVGEEQLGKALEGSDVVIIPAGVPRKPGMTRDDLFNINAGIVKSLCTAIAKYCPNALINMISNPVNSTVPIAAEVFKKAGTYDEKKLFGVTTLDVVRAKTFYAGKANV-PVAEVNVP 205          

HSP 2 Score: 35.039 bits (79), Expect = 2.858e-67
Identity = 14/19 (73.68%), Postives = 16/19 (84.21%), Query Frame = 2
Query:  629 VVGGHAGVTILPLLSQVKP 685
            V+GGHAG+TILPL SQ  P
Sbjct:  206 VIGGHAGITILPLFSQATP 224          
BLAST of CF417249 vs. ExPASy Swiss-Prot
Match: MDHM1_ARATH (Malate dehydrogenase 1, mitochondrial OS=Arabidopsis thaliana GN=At1g53240 PE=1 SV=1)

HSP 1 Score: 239.965 bits (611), Expect = 6.339e-67
Identity = 124/198 (62.63%), Postives = 148/198 (74.75%), Query Frame = 1
Query:   43 NLQMEENSCLRQAKCR---AKGGAAGFKVAILGAAGGIGQPLAMLMKINPLVSVLHLYDVVNTPGVTADISHMDTGAVVRGFLGQPQLENALTGVDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLCEGIAKCCPNATVNLISNPVNSTVPIAAEVFKKAGTYDPKKLLGVTMLDVVRANTFRGGSFGT*SLGDVDVP 627
            ++ +  ++  +QA  R   + G     KVAILGAAGGIGQPLA+LMK+NPLVS L LYD+ NTPGV AD+ H++T + V G++G   L  AL G DLVIIPAGVPRKPGMTRDDLFNINAGIV+ LC  IAK CP+A +N+ISNPVNSTVPIAAE+FKKAG YD KKL GVT LDVVRA TF  G      + +V+VP
Sbjct:    4 SMLVRSSASAKQAVIRRSFSSGSVPERKVAILGAAGGIGQPLALLMKLNPLVSSLSLYDIANTPGVAADVGHINTRSEVVGYMGDDNLAKALEGADLVIIPAGVPRKPGMTRDDLFNINAGIVKNLCTAIAKYCPHALINMISNPVNSTVPIAAEIFKKAGMYDEKKLFGVTTLDVVRARTFYAGKANV-PVAEVNVP 200          

HSP 2 Score: 35.8094 bits (81), Expect = 6.339e-67
Identity = 15/24 (62.50%), Postives = 18/24 (75.00%), Query Frame = 2
Query:  629 VVGGHAGVTILPLLSQVKPPCSFT 700
            V+GGHAGVTILPL SQ  P  + +
Sbjct:  201 VIGGHAGVTILPLFSQATPQANLS 224          
The following BLAST results are available for this feature:
BLAST of CF417249 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 293
Match NameE-valueIdentityDescription
MDHG2_ARATH2.575e-10186.15Probable malate dehydrogenase, glyoxysomal OS=Arab... [more]
MDHG_CITLA1.717e-9685.65Malate dehydrogenase, glyoxysomal OS=Citrullus lan... [more]
MDHG_CUCSA2.928e-9685.65Malate dehydrogenase, glyoxysomal OS=Cucumis sativ... [more]
MDHG_ORYSJ3.351e-9282.21Malate dehydrogenase, glyoxysomal OS=Oryza sativa ... [more]
MDHG_SOYBN5.914e-8979.43Malate dehydrogenase, glyoxysomal OS=Glycine max P... [more]
MDHG1_ARATH4.238e-8779.52Malate dehydrogenase, glyoxysomal OS=Arabidopsis t... [more]
MDHG2_BRANA5.181e-8578.20Malate dehydrogenase 2, glyoxysomal OS=Brassica na... [more]
MDHG1_BRANA2.571e-8478.57Malate dehydrogenase 1, glyoxysomal OS=Brassica na... [more]
MDHM_CITLA2.858e-6770.76Malate dehydrogenase, mitochondrial OS=Citrullus l... [more]
MDHM1_ARATH6.339e-6762.63Malate dehydrogenase 1, mitochondrial OS=Arabidops... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_115000-179 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-003_B05 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF417249 ID=CF417249; Name=CF417249; organism=Citrus sinensis; type=EST; length=700bp
AATCAGCGCATTGCTAGAATCTCTGCTCATCTTTACCCTCCAAATCTCCA
GATGGAGGAGAATTCTTGCTTGAGGCAAGCTAAATGCCGGGCAAAAGGCG
GGGCAGCTGGATTCAAAGTAGCCATCTTGGGTGCTGCTGGAGGCATTGGT
CAACCCCTTGCAATGCTAATGAAGATAAACCCCCTGGTCTCAGTCCTTCA
CCTCTATGATGTTGTCAATACCCCTGGTGTCACTGCTGATATTAGCCATA
TGGACACCGGCGCTGTGGTACGGGGTTTCCTAGGCCAGCCACAGCTTGAG
AATGCTCTCACAGGGGTGGACCTTGTCATTATCCCTGCCGGCGTTCCGAG
GAAGCCTGGAATGACCAGGGATGATCTTTTCAATATCAATGCTGGAATTG
TCAGGACACTCTGTGAAGGCATCGCAAAGTGTTGCCCCAATGCGACTGTC
AATTTGATCAGTAATCCAGTGAACTCTACAGTTCCTATTGCCGCAGAGGT
TTTCAAGAAAGCTGGTACTTATGATCCAAAGAAACTCTTGGGAGTTACAA
TGCTCGATGTCGTAAGAGCCAACACATTCCGTGGCGGAAGTTTTGGGACT
TGATCCCTAGGGGATGTTGATGTTCCTGGTTGTTGGAGGTCATGCTGGAG
TAACAATTTTGCCACTTTTATCACAGGTTAAGCCTCCTTGTAGCTTCACT
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