CF417389 | Citrus Genome Database

Overview


Name	CF417389
Unique Name	CF417389
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	601

Libraries


Library Name	Type
Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CF417389 vs. ExPASy Swiss-Prot
Match: RBS1_CHLRE (Ribulose bisphosphate carboxylase small chain 1, chloroplastic OS=Chlamydomonas reinhardtii GN=RBCS-1 PE=1 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 5.508e-23
Identity = 50/92 (54.35%), Postives = 61/92 (66.30%), Query Frame = 1

Query:  148 MKVWPPTGLKKFETLSYLPPLSDEALLKEISYLLRSGWIPCLEF-ELEKGWVYREHHSSPG-----YYDGRYWTMWKLPMYGCTDATQVLKE 405
            M VW P   K FET SYLPPL+DE +  ++ Y++ +GWIPCLEF E +K +V  E     G     YYD RYWTMWKLPM+GC D  QVL+E
Sbjct:   46 MMVWTPVNNKMFETFSYLPPLTDEQIAAQVDYIVANGWIPCLEFAEADKAYVSNESAIRFGSVSCLYYDNRYWTMWKLPMFGCRDPMQVLRE 137

[Close]

BLAST of CF417389 vs. ExPASy Swiss-Prot
Match: RBS_EUGGR (Ribulose bisphosphate carboxylase small chains, chloroplastic OS=Euglena gracilis GN=RBCS1 PE=2 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.227e-22
Identity = 49/92 (53.26%), Postives = 63/92 (68.48%), Query Frame = 1

Query:  148 MKVWPPTGLKKFETLSYLPPLSDEALLKEISYLLRSGWIPCLEFEL-EKGWVYREHH-----SSPGYYDGRYWTMWKLPMYGCTDATQVLKE 405
            MKVW P   KKFET SYLPPLSD  + K++  ++  G  PCLEF   E  ++  ++      ++ GYYD RYWTMWKLPM+GCTDA+QVL+E
Sbjct:  135 MKVWNPVNNKKFETFSYLPPLSDAQIAKQVDMIIAKGLSPCLEFAAPENSFIANDNTVRFSGTAAGYYDNRYWTMWKLPMFGCTDASQVLRE 226

HSP 2 Score: 106.301 bits (264), Expect = 1.227e-22
Identity = 49/92 (53.26%), Postives = 63/92 (68.48%), Query Frame = 1

Query:  148 MKVWPPTGLKKFETLSYLPPLSDEALLKEISYLLRSGWIPCLEFEL-EKGWVYREHH-----SSPGYYDGRYWTMWKLPMYGCTDATQVLKE 405
            MKVW P   KKFET SYLPPLSD  + K++  ++  G  PCLEF   E  ++  ++      ++ GYYD RYWTMWKLPM+GCTDA+QVL+E
Sbjct:  279 MKVWNPVNNKKFETFSYLPPLSDAQIAKQVDMIIAKGLSPCLEFAAPENSFIANDNTVRFSGTAAGYYDNRYWTMWKLPMFGCTDASQVLRE 370

HSP 3 Score: 106.301 bits (264), Expect = 1.227e-22
Identity = 49/92 (53.26%), Postives = 63/92 (68.48%), Query Frame = 1

Query:  148 MKVWPPTGLKKFETLSYLPPLSDEALLKEISYLLRSGWIPCLEFEL-EKGWVYREHH-----SSPGYYDGRYWTMWKLPMYGCTDATQVLKE 405
            MKVW P   KKFET SYLPPLSD  + K++  ++  G  PCLEF   E  ++  ++      ++ GYYD RYWTMWKLPM+GCTDA+QVL+E
Sbjct:  566 MKVWNPVNNKKFETFSYLPPLSDAQIAKQVDMIIAKGLSPCLEFAAPENSFIANDNTVRFSGTAAGYYDNRYWTMWKLPMFGCTDASQVLRE 657

HSP 4 Score: 106.301 bits (264), Expect = 1.227e-22
Identity = 49/92 (53.26%), Postives = 63/92 (68.48%), Query Frame = 1

Query:  148 MKVWPPTGLKKFETLSYLPPLSDEALLKEISYLLRSGWIPCLEFEL-EKGWVYREHH-----SSPGYYDGRYWTMWKLPMYGCTDATQVLKE 405
            MKVW P   KKFET SYLPPLSD  + K++  ++  G  PCLEF   E  ++  ++      ++ GYYD RYWTMWKLPM+GCTDA+QVL+E
Sbjct:  709 MKVWNPVNNKKFETFSYLPPLSDAQIAKQVDMIIAKGLSPCLEFAAPENSFIANDNTVRFSGTAAGYYDNRYWTMWKLPMFGCTDASQVLRE 800

HSP 5 Score: 106.301 bits (264), Expect = 1.227e-22
Identity = 49/92 (53.26%), Postives = 63/92 (68.48%), Query Frame = 1

Query:  148 MKVWPPTGLKKFETLSYLPPLSDEALLKEISYLLRSGWIPCLEFEL-EKGWVYREHH-----SSPGYYDGRYWTMWKLPMYGCTDATQVLKE 405
            MKVW P   KKFET SYLPPLSD  + K++  ++  G  PCLEF   E  ++  ++      ++ GYYD RYWTMWKLPM+GCTDA+QVL+E
Sbjct:  854 MKVWNPVNNKKFETFSYLPPLSDAQIAKQVDMIIAKGLSPCLEFAAPENSFIANDNTVRFSGTAAGYYDNRYWTMWKLPMFGCTDASQVLRE 945

HSP 6 Score: 106.301 bits (264), Expect = 1.227e-22
Identity = 49/92 (53.26%), Postives = 63/92 (68.48%), Query Frame = 1

Query:  148 MKVWPPTGLKKFETLSYLPPLSDEALLKEISYLLRSGWIPCLEFEL-EKGWVYREHH-----SSPGYYDGRYWTMWKLPMYGCTDATQVLKE 405
            MKVW P   KKFET SYLPPLSD  + K++  ++  G  PCLEF   E  ++  ++      ++ GYYD RYWTMWKLPM+GCTDA+QVL+E
Sbjct:  997 MKVWNPVNNKKFETFSYLPPLSDAQIAKQVDMIIAKGLSPCLEFAAPENSFIANDNTVRFSGTAAGYYDNRYWTMWKLPMFGCTDASQVLRE 1088

HSP 7 Score: 106.301 bits (264), Expect = 1.227e-22
Identity = 49/92 (53.26%), Postives = 63/92 (68.48%), Query Frame = 1

Query:  148 MKVWPPTGLKKFETLSYLPPLSDEALLKEISYLLRSGWIPCLEFEL-EKGWVYREHH-----SSPGYYDGRYWTMWKLPMYGCTDATQVLKE 405
            MKVW P   KKFET SYLPPLSD  + K++  ++  G  PCLEF   E  ++  ++      ++ GYYD RYWTMWKLPM+GCTDA+QVL+E
Sbjct: 1141 MKVWNPVNNKKFETFSYLPPLSDAQIAKQVDMIIAKGLSPCLEFAAPENSFIANDNTVRFSGTAAGYYDNRYWTMWKLPMFGCTDASQVLRE 1232

HSP 8 Score: 104.375 bits (259), Expect = 4.663e-22
Identity = 48/92 (52.17%), Postives = 62/92 (67.39%), Query Frame = 1

Query:  148 MKVWPPTGLKKFETLSYLPPLSDEALLKEISYLLRSGWIPCLEFEL-EKGWVYREHH-----SSPGYYDGRYWTMWKLPMYGCTDATQVLKE 405
            MKVW P   KKFET SYLPPLSD  + K++  ++  G  PCLEF   E  ++  ++      ++ GYYD  YWTMWKLPM+GCTDA+QVL+E
Sbjct:  422 MKVWNPVNNKKFETFSYLPPLSDAQIAKQVDMIIAKGLSPCLEFAAPENSFIANDNTVRFSGTAAGYYDNHYWTMWKLPMFGCTDASQVLRE 513

[Close]

BLAST of CF417389 vs. ExPASy Swiss-Prot
Match: RBS_CHLMO (Ribulose bisphosphate carboxylase small chain, chloroplastic OS=Chlamydomonas moewusii GN=RBCS PE=2 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 2.734e-22
Identity = 45/91 (49.45%), Postives = 61/91 (67.03%), Query Frame = 1

Query:  151 KVWPPTGLKKFETLSYLPPLSDEALLKEISYLLRSGWIPCLEFE------LEKGWVYREHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKE 405
            KVW P   K++ET SYLPPL+++ + +++ Y++ +GW PCLEF       +      R    S GYYD RYWTMWKLPM+GCTD +QVL+E
Sbjct:   30 KVWQPVNNKQYETFSYLPPLTNQKIGRQVDYIINNGWTPCLEFADPSTSFVSNANAVRLQGVSAGYYDNRYWTMWKLPMFGCTDPSQVLRE 120

[Close]

BLAST of CF417389 vs. ExPASy Swiss-Prot
Match: RBS_BATOE (Ribulose bisphosphate carboxylase small chain, chloroplastic OS=Batophora oerstedii GN=RBCS PE=2 SV=2)

HSP 1 Score: 100.908 bits (250), Expect = 5.156e-21
Identity = 49/102 (48.04%), Postives = 65/102 (63.73%), Query Frame = 1

Query:  124 SNG--GRVQCMKVWPPTGLKKFETLSYLPPLSDEALLKEISYLLRSGWIPCLEFE-LEKGWVYREHHSSPG-----YYDGRYWTMWKLPMYGCTDATQVLKE 405
            SNG   + + M VW P   K FET SYLPPL+D+ + K++ Y+LR+ W PCLEF   ++ +V  ++    G     Y D RYWTMWKLPM+GC D +QVL E
Sbjct:   26 SNGTISKSRAMMVWEPFNNKFFETFSYLPPLTDDQITKQVDYILRNNWTPCLEFAGSDQAYVTHDNTVRMGDCASTYQDNRYWTMWKLPMFGCIDGSQVLTE 127

[Close]

BLAST of CF417389 vs. ExPASy Swiss-Prot
Match: RBS1_ACECL (Ribulose bisphosphate carboxylase small chain 1 (Fragment) OS=Acetabularia cliftonii GN=RBCS-1 PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 6.969e-20
Identity = 38/77 (49.35%), Postives = 51/77 (66.23%), Query Frame = 1

Query:  193 SYLPPLSDEALLKEISYLLRSGWIPCLEFEL-EKGWVYREHHSSPG-----YYDGRYWTMWKLPMYGCTDATQVLKE 405
            SYLPPL+DE + K++ Y+L + W PCLEF   ++ +   ++    G     Y D RYWTMWKLPM+GCTD +QVL E
Sbjct:    2 SYLPPLTDEQISKQVDYILANSWTPCLEFAASDQAYAGNDNCIRMGPVASTYQDNRYWTMWKLPMFGCTDGSQVLSE 78

HSP 2 Score: 34.2686 bits (77), Expect = 6.969e-20
Identity = 13/30 (43.33%), Postives = 20/30 (66.67%), Query Frame = 2

Query:  422 KEYPHSFVRIIGFDNKRQVQCISFLAAKPP 511
            K +P +++R++ FD  RQVQ   FL  +PP
Sbjct:   84 KAFPDAYIRLVCFDANRQVQISGFLVHRPP 113

[Close]

BLAST of CF417389 vs. ExPASy Swiss-Prot
Match: RBS_PROHO (Ribulose bisphosphate carboxylase small chain OS=Prochlorothrix hollandica GN=cbbS PE=3 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 3.008e-17
Identity = 31/77 (40.26%), Postives = 46/77 (59.74%), Query Frame = 1

Query:  175 KKFETLSYLPPLSDEALLKEISYLLRSGWIPCLEFELEKGWVYREHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKE 405
            +++ETLSYLPPLSD+ + ++I Y++R G+IP +EF  +                  YWTMWKLP++  T   +VL E
Sbjct:    8 RRYETLSYLPPLSDQQIARQIEYMVREGYIPAVEFNEDSD------------ATTCYWTMWKLPLFHATSTQEVLGE 72

HSP 2 Score: 42.3578 bits (98), Expect = 3.008e-17
Identity = 16/34 (47.06%), Postives = 25/34 (73.53%), Query Frame = 2

Query:  407 VGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKP 508
            V E + EYP+ ++R++GFDN +Q Q +SF+  KP
Sbjct:   73 VRECRTEYPNCYIRVVGFDNIKQCQSVSFIVHKP 106

[Close]

BLAST of CF417389 vs. ExPASy Swiss-Prot
Match: RBS_SYNY3 (Ribulose bisphosphate carboxylase small chain OS=Synechocystis sp. (strain PCC 6803) GN=cbbS PE=3 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 3.198e-16
Identity = 33/77 (42.86%), Postives = 48/77 (62.34%), Query Frame = 1

Query:  175 KKFETLSYLPPLSDEALLKEISYLLRSGWIPCLEFELEKGWVYREHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKE 405
            +++ETLSYLPPL+D+ + K++ +LL  G+IP +EFE +      E H         +WTMWKLP +G   A +VL E
Sbjct:    8 RRYETLSYLPPLTDQQIAKQVEFLLDQGFIPGVEFEEDP---QPETH---------FWTMWKLPFFGGATANEVLAE 72

HSP 2 Score: 39.6614 bits (91), Expect = 3.198e-16
Identity = 16/34 (47.06%), Postives = 24/34 (70.59%), Query Frame = 2

Query:  407 VGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKP 508
            V E + E P+ ++R+IGFDN +Q Q +SF+  KP
Sbjct:   73 VRECRSENPNCYIRVIGFDNIKQCQTVSFIVHKP 106

[Close]

BLAST of CF417389 vs. ExPASy Swiss-Prot
Match: RBS_SYNP6 (Ribulose bisphosphate carboxylase small chain OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) GN=cbbS PE=1 SV=3)

HSP 1 Score: 69.707 bits (169), Expect = 4.159e-16
Identity = 33/77 (42.86%), Postives = 45/77 (58.44%), Query Frame = 1

Query:  175 KKFETLSYLPPLSDEALLKEISYLLRSGWIPCLEFELEKGWVYREHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKE 405
            ++FET SYLPPLSD  +  +I Y++  G+ P +EF           HS+P  +   YWTMWKLP++ C    QVL E
Sbjct:   10 RRFETFSYLPPLSDRQIAAQIEYMIEQGFHPLIEF---------NEHSNPEEF---YWTMWKLPLFDCKSPQQVLDE 74

HSP 2 Score: 35.8094 bits (81), Expect = 4.159e-16
Identity = 14/34 (41.18%), Postives = 22/34 (64.71%), Query Frame = 2

Query:  407 VGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKP 508
            V E + EY   ++R+ GFDN +Q Q +SF+  +P
Sbjct:   75 VRECRSEYGDCYIRVAGFDNIKQCQTVSFIVHRP 108

[Close]

BLAST of CF417389 vs. ExPASy Swiss-Prot
Match: RBS_SINAL (Ribulose bisphosphate carboxylase small chain (Fragment) OS=Sinapis alba GN=RBCS PE=3 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 6.522e-16
Identity = 34/42 (80.95%), Postives = 39/42 (92.86%), Query Frame = 1

Query:  280 ELEKGWVYREHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKE 405
            ELE G+VYREH S+PGYYDGRYWTMWKLP++GCTD+ QVLKE
Sbjct:    1 ELEHGFVYREHGSTPGYYDGRYWTMWKLPLFGCTDSAQVLKE 42

[Close]

BLAST of CF417389 vs. ExPASy Swiss-Prot
Match: RBS2_THIFE (Ribulose bisphosphate carboxylase small chain 2 OS=Thiobacillus ferrooxidans GN=cbbS2 PE=3 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 7.897e-14
Identity = 32/76 (42.11%), Postives = 45/76 (59.21%), Query Frame = 1

Query:  178 KFETLSYLPPLSDEALLKEISYLLRSGWIPCLEFELEKGWVYREHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKE 405
            KFETLSYLP L+ + + ++++Y++  GW P +E            H+ P    G YW MWKLPM+G TD   +LKE
Sbjct:   18 KFETLSYLPALTADEIRQQVAYIVSKGWNPAVE------------HTEPENAFGNYWYMWKLPMFGETDVDTILKE 81

HSP 2 Score: 28.8758 bits (63), Expect = 7.897e-14
Identity = 12/25 (48.00%), Postives = 17/25 (68.00%), Query Frame = 2

Query:  422 KEYPHSFVRIIGFDNKRQVQCISFL 496
            K  PH+ VRI+G+DN +Q Q  S +
Sbjct:   87 KRNPHNHVRIVGYDNFKQSQGTSLV 111

The following BLAST results are available for this feature:

BLAST of CF417389 vs. ExPASy Swiss-Prot

BLAST of CF417389 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 117


Match Name	E-value	Identity	Description
RBS1_CHLRE	5.508e-23	54.35	Ribulose bisphosphate carboxylase small chain 1, c...	[more]
RBS_EUGGR	1.227e-22	53.26	Ribulose bisphosphate carboxylase small chains, ch...	[more]
RBS_CHLMO	2.734e-22	49.45	Ribulose bisphosphate carboxylase small chain, chl...	[more]
RBS_BATOE	5.156e-21	48.04	Ribulose bisphosphate carboxylase small chain, chl...	[more]
RBS1_ACECL	6.969e-20	49.35	Ribulose bisphosphate carboxylase small chain 1 (F...	[more]
RBS_PROHO	3.008e-17	40.26	Ribulose bisphosphate carboxylase small chain OS=P...	[more]
RBS_SYNY3	3.198e-16	42.86	Ribulose bisphosphate carboxylase small chain OS=S...	[more]
RBS_SYNP6	4.159e-16	42.86	Ribulose bisphosphate carboxylase small chain OS=S...	[more]
RBS_SINAL	6.522e-16	80.95	Ribulose bisphosphate carboxylase small chain (Fra...	[more]
RBS2_THIFE	7.897e-14	42.11	Ribulose bisphosphate carboxylase small chain 2 OS...	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_115000-319 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-004_F10 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF417389 ID=CF417389; Name=CF417389; organism=Citrus sinensis; type=EST; length=601bp
GTTGCCACCGCGAACCGCGCCAGCCTTGCTCAGGCCAGCATGGTCGCCCC
CTTCACCGGCCTCAAGTCTTCTTCTGCTTTCCCAGCAACCAAGAAGACCA
ACAATGACATTACTTCCATTGCAAGCAACGGCGGAAGAGTCCAATGCATG
AAGGTGTGGCCACCAACTGGCTTGAAGAAGTTCGAGACTCTCTCATACCT
CCCGCCACTGTCCGATGAAGCTTTGCTCAAGGAAATCAGTTACCTTCTCC
GCTCCGGCTGGATTCCCTGCTTGGAATTCGAGTTGGAGAAAGGATGGGTG
TACCGTGAGCACCACAGCTCACCAGGATACTACGATGGACGCTACTGGAC
AATGTGGAAGCTGCCCATGTATGGGTGCACAGATGCAACCCAGGTTTTGA
AGGAGGGTTGGGGAGGTCCAGAAGGAATACCCACATTCATTCGTCCGAAT
TATCGGATTTGACAACAAGCGTCAAGTGCAGTGCATCAGTTTCCTTGCTG
CCAAGCCACCAGGCGTCTAGGTTCCCAATTAAATTTGTGTAACCACCCAT
GCAATATTGTTGATTATTATTATGTGTAACCCCCATAATGATCATTCGAT
C