CF417623

Overview
NameCF417623
Unique NameCF417623
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length665
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig12296 contig Csv1_Contig12296:118..785. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF417623 vs. ExPASy Swiss-Prot
Match: TPIS_PLAF7 (Triosephosphate isomerase OS=Plasmodium falciparum (isolate 3D7) GN=TPI PE=3 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 1.471e-29
Identity = 56/116 (48.28%), Postives = 79/116 (68.10%), Query Frame = 3 
Query:    6 IGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAYADAIPSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNSVSADVATKTRIIYGGSVNGSNCAELAKQE 353
            + +K   +L   L  + C GE L++RE  KT +V  +Q+KA+ D I ++DNV++AYEP+WAIGTGK ATPEQAQ VH  +R  +K++     A + RI+YGGSVN  NC+ L +QE
Sbjct:  109 VREKLQASLKNNLKAVVCFGESLEQREQNKTIEVITKQVKAFVDLIDNFDNVILAYEPLWAIGTGKTATPEQAQLVHKEIRKIVKDTCGEKQANQIRILYGGSVNTENCSSLIQQE 224

HSP 2 Score: 29.6462 bits (65), Expect = 1.471e-29
Identity = 16/29 (55.17%), Postives = 19/29 (65.52%), Query Frame = 1 
Query:  337 NLQNKRDIDGFLVGGASLKGPEFATIVNS 423
            +L  + DIDGFLVG ASLK   F  I+ S
Sbjct:  219 SLIQQEDIDGFLVGNASLK-ESFVDIIKS 246
[Close]
BLAST of CF417623 vs. ExPASy Swiss-Prot
Match: TPIS_ALKOO (Triosephosphate isomerase OS=Alkaliphilus oremlandii (strain OhILAs) GN=tpiA PE=3 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 1.215e-28
Identity = 55/117 (47.01%), Postives = 75/117 (64.10%), Query Frame = 3 
Query:    6 IGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAYADAIPS--WDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNSVSADVATKTRIIYGGSVNGSNCAELAKQ 350
            + KK   A+ E L  I CIGE L+EREA +TFDV  +QL    + +P+   DNVV+AYEP+WAIGTGK A+ E+AQ V A +R  ++     D++ + RI YGGSV  SN  E+  +
Sbjct:  108 VNKKVKKAIKENLKPIVCIGESLEEREANQTFDVIKKQLLGAFEGVPAEAMDNVVLAYEPIWAIGTGKTASSEEAQTVIAYIRSLIEEKYGVDISEEVRIQYGGSVKASNATEIMNE 224

HSP 2 Score: 37.3502 bits (85), Expect = 1.215e-28
Identity = 17/27 (62.96%), Postives = 20/27 (74.07%), Query Frame = 1 
Query:  340 LQNKRDIDGFLVGGASLKGPEFATIVN 420
            + N+ DIDG LVGGASLK  EF  I+N
Sbjct:  221 IMNETDIDGALVGGASLKAEEFLGIIN 247
[Close]
BLAST of CF417623 vs. ExPASy Swiss-Prot
Match: TPIS_PSEPG (Triosephosphate isomerase OS=Pseudomonas putida (strain GB-1) GN=tpiA PE=3 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 1.699e-27
Identity = 60/114 (52.63%), Postives = 82/114 (71.93%), Query Frame = 3 
Query:    6 IGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAYADA--IPSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNSVSADVATKTRIIYGGSVNGSNCAEL 341
            + +K A A ++GL  + CIGE L+EREAGKT +V  +QL +  +A  + ++ N VIAYEPVWAIGTG  ATP+QAQ+VHAA+R  L  +  A+VA K +++YGGSV  +N AEL
Sbjct:  109 LNRKFAAAQAKGLKPVLCIGETLEEREAGKTLEVVGRQLSSIIEAFGVKAFANAVIAYEPVWAIGTGLTATPQQAQDVHAAIRGQLA-AEDAEVAAKVQLLYGGSVKAANAAEL 221

HSP 2 Score: 29.261 bits (64), Expect = 1.699e-27
Identity = 13/20 (65.00%), Postives = 14/20 (70.00%), Query Frame = 1 
Query:  355 DIDGFLVGGASLKGPEFATI 414
            DIDG L+GGASL   EF  I
Sbjct:  226 DIDGGLIGGASLNADEFGAI 245
[Close]
BLAST of CF417623 vs. ExPASy Swiss-Prot
Match: TPIS_PSEPK (Triosephosphate isomerase OS=Pseudomonas putida (strain KT2440) GN=tpiA PE=3 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 6.351e-27
Identity = 59/114 (51.75%), Postives = 82/114 (71.93%), Query Frame = 3 
Query:    6 IGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAYADA--IPSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNSVSADVATKTRIIYGGSVNGSNCAEL 341
            + +K A A ++GL  + CIGE L+EREAGKT +V  +QL +  +A  + ++ + VIAYEPVWAIGTG  ATP+QAQ+VHAA+R  L  +  A+VA K +++YGGSV  +N AEL
Sbjct:  109 LNRKFAAAQAKGLKPVLCIGETLEEREAGKTLEVVGRQLSSIIEAFGVKAFADAVIAYEPVWAIGTGLTATPQQAQDVHAAIRGQLA-AEDAEVAAKVQLLYGGSVKAANAAEL 221

HSP 2 Score: 29.261 bits (64), Expect = 6.351e-27
Identity = 13/20 (65.00%), Postives = 14/20 (70.00%), Query Frame = 1 
Query:  355 DIDGFLVGGASLKGPEFATI 414
            DIDG L+GGASL   EF  I
Sbjct:  226 DIDGGLIGGASLNADEFGAI 245
[Close]
BLAST of CF417623 vs. ExPASy Swiss-Prot
Match: TPIS_PSEP1 (Triosephosphate isomerase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=tpiA PE=3 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 6.351e-27
Identity = 59/114 (51.75%), Postives = 82/114 (71.93%), Query Frame = 3 
Query:    6 IGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAYADA--IPSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNSVSADVATKTRIIYGGSVNGSNCAEL 341
            + +K A A ++GL  + CIGE L+EREAGKT +V  +QL +  +A  + ++ + VIAYEPVWAIGTG  ATP+QAQ+VHAA+R  L  +  A+VA K +++YGGSV  +N AEL
Sbjct:  109 LNRKFAAAQAKGLKPVLCIGETLEEREAGKTLEVVGRQLSSIIEAFGVKAFADAVIAYEPVWAIGTGLTATPQQAQDVHAAIRGQLA-AEDAEVAAKVQLLYGGSVKAANAAEL 221

HSP 2 Score: 29.261 bits (64), Expect = 6.351e-27
Identity = 13/20 (65.00%), Postives = 14/20 (70.00%), Query Frame = 1 
Query:  355 DIDGFLVGGASLKGPEFATI 414
            DIDG L+GGASL   EF  I
Sbjct:  226 DIDGGLIGGASLNADEFGAI 245
[Close]
BLAST of CF417623 vs. ExPASy Swiss-Prot
Match: TPIS_SHISS (Triosephosphate isomerase OS=Shigella sonnei (strain Ss046) GN=tpiA PE=3 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 8.265e-27
Identity = 60/117 (51.28%), Postives = 73/117 (62.39%), Query Frame = 3 
Query:    6 IGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAY--ADAIPSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNSVSADVATKTRIIYGGSVNGSNCAELAKQ 350
            I KK A    +GL  + CIGE   E EAGKT +VC +Q+ A        +++  VIAYEPVWAIGTGK ATP QAQ VH  +RD +   V A++A +  I YGGSVN SN AEL  Q
Sbjct:  109 IAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFEGAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIA-KVDANIAEQVIIQYGGSVNASNAAELFAQ 224

HSP 2 Score: 35.4242 bits (80), Expect = 8.265e-27
Identity = 18/30 (60.00%), Postives = 20/30 (66.67%), Query Frame = 1 
Query:  355 DIDGFLVGGASLKGPEFATIVNSVTSKKVA 444
            DIDG LVGGASLK   FA IV +  + K A
Sbjct:  226 DIDGALVGGASLKADAFAVIVKAAEAAKQA 255
[Close]
BLAST of CF417623 vs. ExPASy Swiss-Prot
Match: TPIS_SHIFL (Triosephosphate isomerase OS=Shigella flexneri GN=tpiA PE=3 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 8.265e-27
Identity = 60/117 (51.28%), Postives = 73/117 (62.39%), Query Frame = 3 
Query:    6 IGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAY--ADAIPSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNSVSADVATKTRIIYGGSVNGSNCAELAKQ 350
            I KK A    +GL  + CIGE   E EAGKT +VC +Q+ A        +++  VIAYEPVWAIGTGK ATP QAQ VH  +RD +   V A++A +  I YGGSVN SN AEL  Q
Sbjct:  109 IAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFEGAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIA-KVDANIAEQVIIQYGGSVNASNAAELFAQ 224

HSP 2 Score: 35.4242 bits (80), Expect = 8.265e-27
Identity = 18/30 (60.00%), Postives = 20/30 (66.67%), Query Frame = 1 
Query:  355 DIDGFLVGGASLKGPEFATIVNSVTSKKVA 444
            DIDG LVGGASLK   FA IV +  + K A
Sbjct:  226 DIDGALVGGASLKADAFAVIVKAAEAAKQA 255
[Close]
BLAST of CF417623 vs. ExPASy Swiss-Prot
Match: TPIS_SHIF8 (Triosephosphate isomerase OS=Shigella flexneri serotype 5b (strain 8401) GN=tpiA PE=3 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 8.265e-27
Identity = 60/117 (51.28%), Postives = 73/117 (62.39%), Query Frame = 3 
Query:    6 IGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAY--ADAIPSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNSVSADVATKTRIIYGGSVNGSNCAELAKQ 350
            I KK A    +GL  + CIGE   E EAGKT +VC +Q+ A        +++  VIAYEPVWAIGTGK ATP QAQ VH  +RD +   V A++A +  I YGGSVN SN AEL  Q
Sbjct:  109 IAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFEGAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIA-KVDANIAEQVIIQYGGSVNASNAAELFAQ 224

HSP 2 Score: 35.4242 bits (80), Expect = 8.265e-27
Identity = 18/30 (60.00%), Postives = 20/30 (66.67%), Query Frame = 1 
Query:  355 DIDGFLVGGASLKGPEFATIVNSVTSKKVA 444
            DIDG LVGGASLK   FA IV +  + K A
Sbjct:  226 DIDGALVGGASLKADAFAVIVKAAEAAKQA 255
[Close]
BLAST of CF417623 vs. ExPASy Swiss-Prot
Match: TPIS_SHIDS (Triosephosphate isomerase OS=Shigella dysenteriae serotype 1 (strain Sd197) GN=tpiA PE=3 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 8.265e-27
Identity = 60/117 (51.28%), Postives = 73/117 (62.39%), Query Frame = 3 
Query:    6 IGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAY--ADAIPSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNSVSADVATKTRIIYGGSVNGSNCAELAKQ 350
            I KK A    +GL  + CIGE   E EAGKT +VC +Q+ A        +++  VIAYEPVWAIGTGK ATP QAQ VH  +RD +   V A++A +  I YGGSVN SN AEL  Q
Sbjct:  109 IAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFEGAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIA-KVDANIAEQVIIQYGGSVNASNAAELFAQ 224

HSP 2 Score: 35.4242 bits (80), Expect = 8.265e-27
Identity = 18/30 (60.00%), Postives = 20/30 (66.67%), Query Frame = 1 
Query:  355 DIDGFLVGGASLKGPEFATIVNSVTSKKVA 444
            DIDG LVGGASLK   FA IV +  + K A
Sbjct:  226 DIDGALVGGASLKADAFAVIVKAAEAAKQA 255
[Close]
BLAST of CF417623 vs. ExPASy Swiss-Prot
Match: TPIS_SHIBS (Triosephosphate isomerase OS=Shigella boydii serotype 4 (strain Sb227) GN=tpiA PE=3 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 8.265e-27
Identity = 60/117 (51.28%), Postives = 73/117 (62.39%), Query Frame = 3 
Query:    6 IGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAY--ADAIPSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNSVSADVATKTRIIYGGSVNGSNCAELAKQ 350
            I KK A    +GL  + CIGE   E EAGKT +VC +Q+ A        +++  VIAYEPVWAIGTGK ATP QAQ VH  +RD +   V A++A +  I YGGSVN SN AEL  Q
Sbjct:  109 IAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFEGAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIA-KVDANIAEQVIIQYGGSVNASNAAELFAQ 224

HSP 2 Score: 35.4242 bits (80), Expect = 8.265e-27
Identity = 18/30 (60.00%), Postives = 20/30 (66.67%), Query Frame = 1 
Query:  355 DIDGFLVGGASLKGPEFATIVNSVTSKKVA 444
            DIDG LVGGASLK   FA IV +  + K A
Sbjct:  226 DIDGALVGGASLKADAFAVIVKAAEAAKQA 255
The following BLAST results are available for this feature:
BLAST of CF417623 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
TPIS_PLAF71.471e-2948.28Triosephosphate isomerase OS=Plasmodium falciparum... [more]
TPIS_ALKOO1.215e-2847.01Triosephosphate isomerase OS=Alkaliphilus oremland... [more]
TPIS_PSEPG1.699e-2752.63Triosephosphate isomerase OS=Pseudomonas putida (s... [more]
TPIS_PSEPK6.351e-2751.75Triosephosphate isomerase OS=Pseudomonas putida (s... [more]
TPIS_PSEP16.351e-2751.75Triosephosphate isomerase OS=Pseudomonas putida (s... [more]
TPIS_SHISS8.265e-2751.28Triosephosphate isomerase OS=Shigella sonnei (stra... [more]
TPIS_SHIFL8.265e-2751.28Triosephosphate isomerase OS=Shigella flexneri GN=... [more]
TPIS_SHIF88.265e-2751.28Triosephosphate isomerase OS=Shigella flexneri ser... [more]
TPIS_SHIDS8.265e-2751.28Triosephosphate isomerase OS=Shigella dysenteriae ... [more]
TPIS_SHIBS8.265e-2751.28Triosephosphate isomerase OS=Shigella boydii serot... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_115000-553 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-007_D03 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF417623 ID=CF417623; Name=CF417623; organism=Citrus sinensis; type=EST; length=665bp
AGTTTATAGGGAAGAAAGCTGCCTATGCCTTGAGCGAGGGACTTGGTGTC
ATAGCTTGTATCGGGGAACAGTTGCAAGAAAGAGAAGCAGGGAAAACTTT
TGATGTCTGTTTTCAGCAACTTAAAGCCTATGCAGATGCTATACCCAGTT
GGGATAATGTAGTCATTGCATATGAGCCCGTTTGGGCTATTGGAACTGGT
AAAGTGGCCACCCCTGAGCAGGCTCAGGAAGTACATGCTGCTCTTCGTGA
TTGGCTTAAAAACAGTGTCTCAGCAGACGTTGCCACTAAAACACGTATTA
TCTATGGAGGGTCAGTAAATGGAAGCAATTGTGCTGAACTTGCAAAACAA
GAGGGATATTGATGGTTTTCTGGTTGGTGGTGCTTCCTTGAAGGGCCCAG
AGTTTGCCACCATTGTCAATTCCGTAACATCCAAGAAAGTAGCTGCGTGA
TGTATAAACCTGTTTTGTCAAGATCAACCAATAATGGGCTAAGAGAATCT
ATATCAGTTTAAAGCTAATTATCGTAACCAGCCTATGCCCTTGCTATGTT
CAACGAAGTTTTGATAGTTTATTTATTTGTTTCTCCTTCCCCNAACAATC
GGAGGTTCCTTCTGTTTCTCTATTGTGGGCTGAACTTGTTTGATACCGGA
CAGGAGAACAATATA
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