CF504207 | Citrus Genome Database

Overview


Name	CF504207
Unique Name	CF504207
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	472

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig2225	contig	Csv1_Contig2225:231..703.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CF504207 vs. ExPASy Swiss-Prot
Match: UBC2_CAEEL (Ubiquitin-conjugating enzyme E2 2 OS=Caenorhabditis elegans GN=let-70 PE=1 SV=1)

HSP 1 Score: 206.068 bits (523), Expect = 1.885e-59
Identity = 92/115 (80.00%), Postives = 102/115 (88.70%), Query Frame = 1

Query:   73 WQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 417
            WQATIMGP +SPY GGVF +TIHFP DYPFKPPKVAF T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ +Y   AR WTQKYAM
Sbjct:   33 WQATIMGPPESPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRERYNQLAREWTQKYAM 147

HSP 2 Score: 43.1282 bits (100), Expect = 1.885e-59
Identity = 17/31 (54.84%), Postives = 22/31 (70.97%), Query Frame = 3

Query:    3 LKDLQKDPPTSCSAGPVAEDMFHLASNNYGP 95
            L+DL +DPP  CSAGPV +D+FH  +   GP
Sbjct:   10 LQDLGRDPPAQCSAGPVGDDLFHWQATIMGP 40

[Close]

BLAST of CF504207 vs. ExPASy Swiss-Prot
Match: UB2D3_BOVIN (Ubiquitin-conjugating enzyme E2 D3 OS=Bos taurus GN=UBE2D3 PE=2 SV=1)

HSP 1 Score: 204.912 bits (520), Expect = 1.885e-59
Identity = 92/115 (80.00%), Postives = 102/115 (88.70%), Query Frame = 1

Query:   73 WQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 417
            WQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVAF T+++HPNINSN SICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   +R WTQKYAM
Sbjct:   33 WQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNDSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM 147

HSP 2 Score: 44.2838 bits (103), Expect = 1.885e-59
Identity = 18/31 (58.06%), Postives = 21/31 (67.74%), Query Frame = 3

Query:    3 LKDLQKDPPTSCSAGPVAEDMFHLASNNYGP 95
            L DL +DPP  CSAGPV +DMFH  +   GP
Sbjct:   10 LSDLARDPPAQCSAGPVGDDMFHWQATIMGP 40

[Close]

BLAST of CF504207 vs. ExPASy Swiss-Prot
Match: UBC12_ARATH (Probable ubiquitin-conjugating enzyme E2 12 OS=Arabidopsis thaliana GN=UBC12 PE=2 SV=1)

HSP 1 Score: 207.994 bits (528), Expect = 2.457e-59
Identity = 96/116 (82.76%), Postives = 105/116 (90.52%), Query Frame = 1

Query:   73 WQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAMG 420
            WQATIMGP DSPY+GGVF V+I F  DYPFKPPKV F+TKV+HPNI+S GSICLDILKEQWSPA T SKVLLSICSLLTDPNP+DPLVPEIAH+YK DK+KYESTA+ WTQKYAMG
Sbjct:   34 WQATIMGPHDSPYSGGVFTVSIDFSSDYPFKPPKVNFKTKVYHPNIDSKGSICLDILKEQWSPAPTTSKVLLSICSLLTDPNPNDPLVPEIAHLYKVDKSKYESTAQKWTQKYAMG 149

HSP 2 Score: 40.817 bits (94), Expect = 2.457e-59
Identity = 18/32 (56.25%), Postives = 24/32 (75.00%), Query Frame = 3

Query:    3 LKDLQKDPPTSCSAGPVA-EDMFHLASNNYGP 95
            L+D+Q+ PP +CSAGPVA ED+FH  +   GP
Sbjct:   10 LRDMQRHPPANCSAGPVAEEDIFHWQATIMGP 41

[Close]

BLAST of CF504207 vs. ExPASy Swiss-Prot
Match: U2D2B_RAT (Ubiquitin-conjugating enzyme E2 D2B OS=Rattus norvegicus GN=Ube2d2b PE=2 SV=1)

HSP 1 Score: 198.364 bits (503), Expect = 1.008e-57
Identity = 89/115 (77.39%), Postives = 100/115 (86.96%), Query Frame = 1

Query:   73 WQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 417
            WQATIMGP+DSPY GG F +TI FP +YPFKPPKV F T+++HPN+NSNGSICLDIL+ QWSPALTISKVLLSI SLL DPNPDDPLVPEIA +YK+D+ KY  TAR WTQKYAM
Sbjct:   33 WQATIMGPNDSPYQGGAFFLTIDFPTEYPFKPPKVEFTTRIYHPNVNSNGSICLDILRSQWSPALTISKVLLSISSLLCDPNPDDPLVPEIAQIYKTDRDKYNRTAREWTQKYAM 147

HSP 2 Score: 45.0542 bits (105), Expect = 1.008e-57
Identity = 19/31 (61.29%), Postives = 21/31 (67.74%), Query Frame = 3

Query:    3 LKDLQKDPPTSCSAGPVAEDMFHLASNNYGP 95
            L DL +DPP  CSAGPV EDMFH  +   GP
Sbjct:   10 LNDLAQDPPAQCSAGPVGEDMFHWQATIMGP 40

[Close]

BLAST of CF504207 vs. ExPASy Swiss-Prot
Match: U2D2B_MOUSE (Ubiquitin-conjugating enzyme E2 D2B OS=Mus musculus GN=Ube2d2b PE=2 SV=1)

HSP 1 Score: 198.364 bits (503), Expect = 1.008e-57
Identity = 89/115 (77.39%), Postives = 100/115 (86.96%), Query Frame = 1

Query:   73 WQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 417
            WQATIMGP+DSPY GG F +TI FP +YPFKPPKV F T+++HPN+NSNGSICLDIL+ QWSPALTISKVLLSI SLL DPNPDDPLVPEIA +YK+D+ KY  TAR WTQKYAM
Sbjct:   33 WQATIMGPNDSPYQGGAFFLTIDFPTEYPFKPPKVEFTTRIYHPNVNSNGSICLDILRSQWSPALTISKVLLSISSLLCDPNPDDPLVPEIAQIYKTDRDKYNRTAREWTQKYAM 147

HSP 2 Score: 45.0542 bits (105), Expect = 1.008e-57
Identity = 19/31 (61.29%), Postives = 21/31 (67.74%), Query Frame = 3

Query:    3 LKDLQKDPPTSCSAGPVAEDMFHLASNNYGP 95
            L DL +DPP  CSAGPV EDMFH  +   GP
Sbjct:   10 LNDLAQDPPAQCSAGPVGEDMFHWQATIMGP 40

[Close]

BLAST of CF504207 vs. ExPASy Swiss-Prot
Match: UB2E2_MOUSE (Ubiquitin-conjugating enzyme E2 E2 OS=Mus musculus GN=Ube2e2 PE=2 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 3.191e-43
Identity = 75/114 (65.79%), Postives = 91/114 (79.82%), Query Frame = 1

Query:   73 WQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 414
            W++TI+GP  S Y GGVF + I F PDYPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y +++A+++  AR WT++YA
Sbjct:   87 WRSTILGPPGSVYEGGVFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYA 200

HSP 2 Score: 31.187 bits (69), Expect = 3.191e-43
Identity = 12/31 (38.71%), Postives = 19/31 (61.29%), Query Frame = 3

Query:    3 LKDLQKDPPTSCSAGPVAEDMFHLASNNYGP 95
            L ++  DPP +CSAGP  ++++   S   GP
Sbjct:   64 LAEITLDPPPNCSAGPKGDNIYEWRSTILGP 94

[Close]

BLAST of CF504207 vs. ExPASy Swiss-Prot
Match: UB2E2_HUMAN (Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens GN=UBE2E2 PE=1 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 3.191e-43
Identity = 75/114 (65.79%), Postives = 91/114 (79.82%), Query Frame = 1

Query:   73 WQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 414
            W++TI+GP  S Y GGVF + I F PDYPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y +++A+++  AR WT++YA
Sbjct:   87 WRSTILGPPGSVYEGGVFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYA 200

HSP 2 Score: 31.187 bits (69), Expect = 3.191e-43
Identity = 12/31 (38.71%), Postives = 19/31 (61.29%), Query Frame = 3

Query:    3 LKDLQKDPPTSCSAGPVAEDMFHLASNNYGP 95
            L ++  DPP +CSAGP  ++++   S   GP
Sbjct:   64 LAEITLDPPPNCSAGPKGDNIYEWRSTILGP 94

[Close]

BLAST of CF504207 vs. ExPASy Swiss-Prot
Match: UB2E1_MOUSE (Ubiquitin-conjugating enzyme E2 E1 OS=Mus musculus GN=Ube2e1 PE=2 SV=1)

HSP 1 Score: 162.155 bits (409), Expect = 3.194e-43
Identity = 74/114 (64.91%), Postives = 91/114 (79.82%), Query Frame = 1

Query:   73 WQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 414
            W++TI+GP  S Y GGVF + I F P+YPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y +++A+++  AR WT++YA
Sbjct:   79 WRSTILGPPGSVYEGGVFFLDITFTPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYA 192

HSP 2 Score: 32.7278 bits (73), Expect = 3.194e-43
Identity = 13/31 (41.94%), Postives = 19/31 (61.29%), Query Frame = 3

Query:    3 LKDLQKDPPTSCSAGPVAEDMFHLASNNYGP 95
            L D+  DPP +CSAGP  ++++   S   GP
Sbjct:   56 LADITLDPPPNCSAGPKGDNIYEWRSTILGP 86

[Close]

BLAST of CF504207 vs. ExPASy Swiss-Prot
Match: UB2E1_HUMAN (Ubiquitin-conjugating enzyme E2 E1 OS=Homo sapiens GN=UBE2E1 PE=1 SV=1)

HSP 1 Score: 162.155 bits (409), Expect = 3.194e-43
Identity = 74/114 (64.91%), Postives = 91/114 (79.82%), Query Frame = 1

Query:   73 WQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 414
            W++TI+GP  S Y GGVF + I F P+YPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y +++A+++  AR WT++YA
Sbjct:   79 WRSTILGPPGSVYEGGVFFLDITFTPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYA 192

HSP 2 Score: 32.7278 bits (73), Expect = 3.194e-43
Identity = 13/31 (41.94%), Postives = 19/31 (61.29%), Query Frame = 3

Query:    3 LKDLQKDPPTSCSAGPVAEDMFHLASNNYGP 95
            L D+  DPP +CSAGP  ++++   S   GP
Sbjct:   56 LADITLDPPPNCSAGPKGDNIYEWRSTILGP 86

[Close]

BLAST of CF504207 vs. ExPASy Swiss-Prot
Match: UBCD2_DROME (Ubiquitin-conjugating enzyme E2-24 kDa OS=Drosophila melanogaster GN=UbcD2 PE=1 SV=1)

HSP 1 Score: 161.384 bits (407), Expect = 9.181e-43
Identity = 74/114 (64.91%), Postives = 89/114 (78.07%), Query Frame = 1

Query:   73 WQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 414
            W +TI+GP  S Y GGVF + IHF P+YPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y  ++ +++  AR WT++YA
Sbjct:  118 WVSTILGPPGSVYEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLQNREEHDRIARLWTKRYA 231

HSP 2 Score: 31.9574 bits (71), Expect = 9.181e-43
Identity = 12/31 (38.71%), Postives = 19/31 (61.29%), Query Frame = 3

Query:    3 LKDLQKDPPTSCSAGPVAEDMFHLASNNYGP 95
            L ++  DPP +CSAGP  ++++   S   GP
Sbjct:   95 LAEITLDPPPNCSAGPKGDNLYEWVSTILGP 125

The following BLAST results are available for this feature:

BLAST of CF504207 vs. ExPASy Swiss-Prot

BLAST of CF504207 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 251


Match Name	E-value	Identity	Description
UBC2_CAEEL	1.885e-59	80.00	Ubiquitin-conjugating enzyme E2 2 OS=Caenorhabditi...	[more]
UB2D3_BOVIN	1.885e-59	80.00	Ubiquitin-conjugating enzyme E2 D3 OS=Bos taurus G...	[more]
UBC12_ARATH	2.457e-59	82.76	Probable ubiquitin-conjugating enzyme E2 12 OS=Ara...	[more]
U2D2B_RAT	1.008e-57	77.39	Ubiquitin-conjugating enzyme E2 D2B OS=Rattus norv...	[more]
U2D2B_MOUSE	1.008e-57	77.39	Ubiquitin-conjugating enzyme E2 D2B OS=Mus musculu...	[more]
UB2E2_MOUSE	3.191e-43	65.79	Ubiquitin-conjugating enzyme E2 E2 OS=Mus musculus...	[more]
UB2E2_HUMAN	3.191e-43	65.79	Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens...	[more]
UB2E1_MOUSE	3.194e-43	64.91	Ubiquitin-conjugating enzyme E2 E1 OS=Mus musculus...	[more]
UB2E1_HUMAN	3.194e-43	64.91	Ubiquitin-conjugating enzyme E2 E1 OS=Homo sapiens...	[more]
UBCD2_DROME	9.181e-43	64.91	Ubiquitin-conjugating enzyme E2-24 kDa OS=Drosophi...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_119000-695 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-15_B11 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF504207 ID=CF504207; Name=CF504207; organism=Citrus sinensis; type=EST; length=472bp
AACTCAAGGATTTGCAGAAGGATCCTCCCACGTCTTGCAGCGCTGGCCCT
GTGGCTGAAGACATGTTCCACCTGGCAAGCAACAATTATGGGCCCTTCTG
ATAGCCCATATGCAGGAGGTGTATTTTTAGTTACCATTCATTTTCCTCCT
GATTATCCTTTCAAGCCCCCAAAGGTTGCTTTTAGGACCAAGGTCTTCCA
CCCGAACATCAACAGCAATGGAAGCATCTGTCTTGATATCCTTAAAGAAC
AGTGGAGTCCAGCGCTTACCATTTCAAAGGTCCTGCTCTCTATCTGCTCC
TTGCTTACTGATCCAAATCCCGACGACCCTCTTGTACCGGAGATTGCCCA
CATGTACAAGTCCGACAAGGCCAAATATGAATCCACGGCCAGGAGCTGGA
CACAGAAGTATGCCATGGGATGAAGGGTGTTAGGATATTGCAGAAAAAGG
AGCATTTATTGAAATATGGTGG