CF835663

Overview
NameCF835663
Unique NameCF835663
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length684
Libraries
Library NameType
Washington Navel Orange Shoot Meristem cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF835663 vs. ExPASy Swiss-Prot
Match: CCD1_PHAVU (Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Phaseolus vulgaris GN=CCD1 PE=1 SV=1)

HSP 1 Score: 357.836 bits (917), Expect = 2.972e-98
Identity = 173/208 (83.17%), Postives = 187/208 (89.90%), Query Frame = 2
Query:   59 GARNGIVAVNPKPKNGVTSKVIDFVEKLIVKWMYDSSQPLHYLSGNFAPVPDETPPTKDLHVIGRLPDCLNGEFVRVGPNPKFAPVAGYHWFDGDGMIHGLRIKDGKATYVSRFVRTSRLKQEEFFGGAKFMKIGDLKGLFGLLMVNMQMLRAKLKVLDVSYGNGTANTALVYHHGKLLALSEADKPYAVKVLEDGDLQTLGMIDYDK 682
            GA  G+V V+PKP NG +SKVID +EKL+VK++YDSS P HYL+GNF PV  ETPPTKDL V G LPDCLNGEFVRVGPNPKFAPVAGYHWFDGDGMIHGLRIKDGKATYVSRFV TSRLKQEE+FG +KFMKIGDLKGLFGLLMVN+ MLR KLKVLD+SYG GT NTALVYHHGKLLALSEADKPYA+KV EDGDLQTLGM+DYDK
Sbjct:    9 GAEGGLVKVDPKPTNGFSSKVIDLLEKLLVKFLYDSSLPHHYLTGNFGPVT-ETPPTKDLPVKGHLPDCLNGEFVRVGPNPKFAPVAGYHWFDGDGMIHGLRIKDGKATYVSRFVETSRLKQEEYFGRSKFMKIGDLKGLFGLLMVNIHMLRTKLKVLDLSYGGGTTNTALVYHHGKLLALSEADKPYAIKVFEDGDLQTLGMLDYDK 215          
BLAST of CF835663 vs. ExPASy Swiss-Prot
Match: CCD1_PEA (Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Pisum sativum GN=CCD1 PE=2 SV=1)

HSP 1 Score: 357.451 bits (916), Expect = 3.882e-98
Identity = 174/215 (80.93%), Postives = 188/215 (87.44%), Query Frame = 2
Query:   38 EKLENDVGARNGIVAVNPKPKNGVTSKVIDFVEKLIVKWMYDSSQPLHYLSGNFAPVPDETPPTKDLHVIGRLPDCLNGEFVRVGPNPKFAPVAGYHWFDGDGMIHGLRIKDGKATYVSRFVRTSRLKQEEFFGGAKFMKIGDLKGLFGLLMVNMQMLRAKLKVLDVSYGNGTANTALVYHHGKLLALSEADKPYAVKVLEDGDLQTLGMIDYDK 682
            EK EN V     I+ V PKP NG TSK +D +EK+IVK  YDSS P H+LSGNFAPV DETPP KDL V G LPDCLNGEFVRVGPNPKF+PVAGYHWFDGDGMIHGLRIKDGKA+YVSRFV+TSR KQEE+F G+KFMKIGDLKGLFGLLMVNMQMLRAKLK+LDVSYG+GTANTALVYHH KLLALSE DKPYA+KV EDGDLQTLGM+DYDK
Sbjct:    4 EKKENGV-----ILEVEPKPSNGFTSKAVDLLEKIIVKLFYDSSLPHHWLSGNFAPVKDETPPVKDLTVQGHLPDCLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKASYVSRFVKTSRFKQEEYFNGSKFMKIGDLKGLFGLLMVNMQMLRAKLKILDVSYGHGTANTALVYHHQKLLALSEGDKPYAIKVFEDGDLQTLGMLDYDK 213          
BLAST of CF835663 vs. ExPASy Swiss-Prot
Match: CCD1_ARATH (Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Arabidopsis thaliana GN=CCD1 PE=1 SV=2)

HSP 1 Score: 344.354 bits (882), Expect = 3.401e-94
Identity = 161/205 (78.54%), Postives = 184/205 (89.76%), Query Frame = 2
Query:   68 NGIVAVNPKPKNGVTSKVIDFVEKLIVKWMYDSSQPLHYLSGNFAPVPDETPPTKDLHVIGRLPDCLNGEFVRVGPNPKFAPVAGYHWFDGDGMIHGLRIKDGKATYVSRFVRTSRLKQEEFFGGAKFMKIGDLKGLFGLLMVNMQMLRAKLKVLDVSYGNGTANTALVYHHGKLLALSEADKPYAVKVLEDGDLQTLGMIDYDK 682
            + I++V+P+P  G +SK++D +E+L+VK M+D+S PLHYLSGNFAP+ DETPP KDL V G LP+CLNGEFVRVGPNPKF  VAGYHWFDGDGMIHG+RIKDGKATYVSR+V+TSRLKQEEFFG AKFMKIGDLKG FGLLMVN+Q LR KLK+LD +YGNGTANTALVYHHGKLLAL EADKPY +KVLEDGDLQTLG+IDYDK
Sbjct:    9 SSIISVHPRPSKGFSSKLLDLLERLVVKLMHDASLPLHYLSGNFAPIRDETPPVKDLPVHGFLPECLNGEFVRVGPNPKFDAVAGYHWFDGDGMIHGVRIKDGKATYVSRYVKTSRLKQEEFFGAAKFMKIGDLKGFFGLLMVNVQQLRTKLKILDNTYGNGTANTALVYHHGKLLALQEADKPYVIKVLEDGDLQTLGIIDYDK 213          
BLAST of CF835663 vs. ExPASy Swiss-Prot
Match: CCD_CROSA (Carotenoid 9,10(9',10')-cleavage dioxygenase OS=Crocus sativus GN=CCD PE=1 SV=1)

HSP 1 Score: 340.887 bits (873), Expect = 3.760e-93
Identity = 161/215 (74.88%), Postives = 180/215 (83.72%), Query Frame = 2
Query:   38 EKLENDVGARNGIVAVNPKPKNGVTSKVIDFVEKLIVKWMYDSSQPLHYLSGNFAPVPDETPPTKDLHVIGRLPDCLNGEFVRVGPNPKFAPVAGYHWFDGDGMIHGLRIKDGKATYVSRFVRTSRLKQEEFFGGAKFMKIGDLKGLFGLLMVNMQMLRAKLKVLDVSYGNGTANTALVYHHGKLLALSEADKPYAVKVLEDGDLQTLGMIDYDK 682
            E  + +V  R  IVAVNP+P  G+ S  +D +EK +V   +D S+P HYLSGNFAPV DETPP  DL V G LP+CLNGEFVRVGPNPKF PVAGYHWFDGDGMIHG+RIKDGKATY SR+V+TSRLKQEE+F G KFMKIGDLKG FGL MV MQ+LRAKLKV+DVSYG GT NTAL+YHHGKLLALSEADKPY VKVLEDGDLQTLG++DYDK
Sbjct:    3 EVAKEEVEERRSIVAVNPQPSKGLVSSAVDLIEKAVVYLFHDKSKPCHYLSGNFAPVVDETPPCPDLPVRGHLPECLNGEFVRVGPNPKFMPVAGYHWFDGDGMIHGMRIKDGKATYASRYVKTSRLKQEEYFEGPKFMKIGDLKGFFGLFMVQMQLLRAKLKVIDVSYGVGTGNTALIYHHGKLLALSEADKPYVVKVLEDGDLQTLGLLDYDK 217          
BLAST of CF835663 vs. ExPASy Swiss-Prot
Match: NCED5_ARATH (Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Arabidopsis thaliana GN=NCED5 PE=1 SV=1)

HSP 1 Score: 161.384 bits (407), Expect = 4.083e-39
Identity = 84/201 (41.79%), Postives = 123/201 (61.19%), Query Frame = 2
Query:   95 PKNGVTSKVIDFVEKLIV-----KWMYDSSQPLHYLSGNFAPVPDETPPTKDLHVIGRLPDCLNGEFVRVGPNPKFAPVAGYHWFDGDGMIHGLRIKDGKATYVSRFVRTSRLKQEEFFGGAKFMK-IGDLKGLFGLLMVNMQMLRAKLKVLDVSYGNGTANTALVYHHGKLLALSEADKPYAVKVLEDGDLQTLGMIDYD 679
            P     S  +DF E  ++     K +  +  P H +SGN+APVP+++  +  L V G++PDC++G ++R G NP F PV+G+H FDGDGM+H ++I +G A+Y  RF  T RL QE+  G   F K IG+L G  G+  + +   R    +L+   G G AN  LVY H +LLA+SE D PY V+V ++GDL+T+G  D+D
Sbjct:   78 PLQRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVPEQSVKSS-LSVDGKIPDCIDGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKITNGDASYSCRFTETERLVQEKQLGSPIFPKAIGELHGHSGIARLMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDLPYQVRVTDNGDLETIGRFDFD 277          
BLAST of CF835663 vs. ExPASy Swiss-Prot
Match: NCED1_MAIZE (9-cis-epoxycarotenoid dioxygenase 1, chloroplastic OS=Zea mays GN=VP14 PE=1 SV=2)

HSP 1 Score: 158.688 bits (400), Expect = 2.647e-38
Identity = 84/174 (48.28%), Postives = 114/174 (65.52%), Query Frame = 2
Query:  164 SSQPLHYLSGNFAPVPDETPPTKDLHVIGRLPDCLNGEFVRVGPNPKFAPVAGYHWFDGDGMIHGLRIKDGKA-TYVSRFVRTSRLKQEEFFGGAKFMK-IGDLKGLFGLLMVNMQMLRAKLKVLDVSYGNGTANTALVYHHGKLLALSEADKPYAVKVLEDGDLQTLGMIDYD 679
            ++ P   ++GNFAPV  E PP  +L V GR+P  ++G + R G NP F PVAG+H FDGDGM+H LRI++G A +Y  RF  T+RL+QE   G   F K IG+L G  G+  + +   RA   ++D S G G AN  LVY +G+LLA+SE D PY V+V +DGDL+T+G  D+D
Sbjct:  116 TADPAVQIAGNFAPV-GERPPVHELPVSGRIPPFIDGVYARNGANPCFDPVAGHHLFDGDGMVHALRIRNGAAESYACRFTETARLRQERAIGRPVFPKAIGELHGHSGIARLALFYARAACGLVDPSAGTGVANAGLVYFNGRLLAMSEDDLPYHVRVADDGDLETVGRYDFD 288          
BLAST of CF835663 vs. ExPASy Swiss-Prot
Match: NCED3_ARATH (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana GN=NCED3 PE=2 SV=1)

HSP 1 Score: 154.451 bits (389), Expect = 4.991e-37
Identity = 84/217 (38.71%), Postives = 127/217 (58.53%), Query Frame = 2
Query:   77 VAVNPKPKNGVTSKV----------IDFVEKLIVKW-----MYDSSQPLHYLSGNFAPVPDETPPTKDLHVIGRLPDCLNGEFVRVGPNPKFAPVAGYHWFDGDGMIHGLRIKDGKATYVSRFVRTSRLKQEEFFGGAKFMK-IGDLKGLFGLLMVNMQMLRAKLKVLDVSYGNGTANTALVYHHGKLLALSEADKPYAVKVLEDGDLQTLGMIDYD 679
            + V PK K   T ++          +D  E  +V       +  ++ P   ++GNFAPV +E P  ++L V+G+LPD + G +VR G NP   PV G+H+FDGDGM+H ++ + G A+Y  RF +T+R  QE   G   F K IG+L G  G+  + +   RA   ++D ++G G AN  LVY +G+LLA+SE D PY V++  +GDL+T+G  D+D
Sbjct:   72 IVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQIAGNFAPV-NEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKFEHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLFYARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFD 287          
BLAST of CF835663 vs. ExPASy Swiss-Prot
Match: NCED2_ARATH (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Arabidopsis thaliana GN=NCED2 PE=2 SV=1)

HSP 1 Score: 151.369 bits (381), Expect = 4.225e-36
Identity = 79/192 (41.15%), Postives = 118/192 (61.46%), Query Frame = 2
Query:  122 IDFVEKLIVKWMYDS-----SQPLHYLSGNFAPVPDETPPTKDLHVIGRLPDCLNGEFVRVGPNPKFAPVAGYHWFDGDGMIHGLRIKDGKATYVSRFVRTSRLKQEEFFGGAKFMK-IGDLKGLFGLLMVNMQMLRAKLKVLDVSYGNGTANTALVYHHGKLLALSEADKPYAVKVLEDGDLQTLGMIDYD 679
            ID  E+ ++    DS     + P   ++GN++PVP E+   ++L V G +PDC++G ++R G NP F P AG+H FDGDGM+H ++I +G A+Y  RF +T RL QE+  G   F K IG+L G  G+  + +   R    +++   G G AN  LVY + +LLA+SE D PY +K+ + GDLQT+G  D+D
Sbjct:   80 IDAAERALISHEQDSPLPKTADPRVQIAGNYSPVP-ESSVRRNLTVEGTIPDCIDGVYIRNGANPMFEPTAGHHLFDGDGMVHAVKITNGSASYACRFTKTERLVQEKRLGRPVFPKAIGELHGHSGIARLMLFYARGLCGLINNQNGVGVANAGLVYFNNRLLAMSEDDLPYQLKITQTGDLQTVGRYDFD 270          
BLAST of CF835663 vs. ExPASy Swiss-Prot
Match: NCED1_PHAVU (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris GN=NCED1 PE=2 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 5.518e-36
Identity = 80/194 (41.24%), Postives = 120/194 (61.86%), Query Frame = 2
Query:  122 IDFVEKLIVKWMYDSSQPLH-------YLSGNFAPVPDETPPTKDLHVIGRLPDCLNGEFVRVGPNPKFAPVAGYHWFDGDGMIHGLRIKDGKATYVSRFVRTSRLKQEEFFGGAKFMK-IGDLKGLFGLLMVNMQMLRAKLKVLDVSYGNGTANTALVYHHGKLLALSEADKPYAVKVLEDGDLQTLGMIDYD 679
            +D VE  +V   ++S  PL         ++GNFAPVP+     + L V+G++P C++G +VR G NP + PVAG+H+FDGDGM+H ++  +G A+Y  RF  T RL QE+  G   F K IG+L G  G+  + +   R+  +++D S+G G AN  LVY +  LLA+SE D PY V++  +GDL T+G  D++
Sbjct:  116 LDMVETALVS--HESKHPLPKTADPKVQIAGNFAPVPEHAAD-QALPVVGKIPKCIDGVYVRNGANPLYEPVAGHHFFDGDGMVHAVKFTNGAASYACRFTETQRLAQEKSLGRPVFPKAIGELHGHSGIARLLLFYARSLFQLVDGSHGMGVANAGLVYFNNHLLAMSEDDLPYHVRITSNGDLTTVGRYDFN 306          
BLAST of CF835663 vs. ExPASy Swiss-Prot
Match: NCED9_ARATH (9-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic OS=Arabidopsis thaliana GN=NCED9 PE=2 SV=1)

HSP 1 Score: 148.288 bits (373), Expect = 3.577e-35
Identity = 85/207 (41.06%), Postives = 117/207 (56.52%), Query Frame = 2
Query:   86 NPK--PKNGVTSKVIDFVEKLIVKWMY------DSSQPLHYLSGNFAPVPDETPPTKDLHVIGRLPDCLNGEFVRVGPNPKFAPVAGYHWFDGDGMIHGLRIKDGKATYVSRFVRTSRLKQEEFFGGAKFMK-IGDLKGLFGLLMVNMQMLRAKLKVLDVSYGNGTANTALVYHHGKLLALSEADKPYAVKVLEDGDLQTLGMIDYD 679
            NPK  P     + V+D VE  ++           ++ P   ++GNF PVP E P   +L V G +P+C+ G +VR G NP   PV+G+H FDGDGM+H +R  +G  +Y  RF  T+RL QE   G   F K IG+L G  G+  + +   R    ++D + G G AN  LVY +G LLA+SE D PY VKV + GDL+T G  D+D
Sbjct:  142 NPKLNPVQRTAAMVLDAVENAMISHERRRHPHPKTADPAVQIAGNFFPVP-EKPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLFDGDGMVHAVRFDNGSVSYACRFTETNRLVQERECGRPVFPKAIGELHGHLGIAKLMLFNTRGLFGLVDPTGGLGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFD 347          
The following BLAST results are available for this feature:
BLAST of CF835663 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 12
Match NameE-valueIdentityDescription
CCD1_PHAVU2.972e-9883.17Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=... [more]
CCD1_PEA3.882e-9880.93Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=... [more]
CCD1_ARATH3.401e-9478.54Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=... [more]
CCD_CROSA3.760e-9374.88Carotenoid 9,10(9',10')-cleavage dioxygenase OS=Cr... [more]
NCED5_ARATH4.083e-3941.79Probable 9-cis-epoxycarotenoid dioxygenase NCED5, ... [more]
NCED1_MAIZE2.647e-3848.289-cis-epoxycarotenoid dioxygenase 1, chloroplastic... [more]
NCED3_ARATH4.991e-3738.719-cis-epoxycarotenoid dioxygenase NCED3, chloropla... [more]
NCED2_ARATH4.225e-3641.159-cis-epoxycarotenoid dioxygenase NCED2, chloropla... [more]
NCED1_PHAVU5.518e-3641.249-cis-epoxycarotenoid dioxygenase NCED1, chloropla... [more]
NCED9_ARATH3.577e-3541.069-cis-epoxycarotenoid dioxygenase NCED9, chloropla... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionUCRCS03_01K15_r Washington Navel Orange Shoot Meristem cDNA Library Citrus sinensis cDNA clone CS_PEa01K15, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF835663 ID=CF835663; Name=CF835663; organism=Citrus sinensis; type=EST; length=684bp
GAGACAGAGACCCATTTAGAGAGTCATGGTGGAGAAAGAAAAGCTGGAGA
ACGACGTCGGAGCACGAAATGGAATAGTGGCGGTGAATCCCAAACCGAAG
AATGGAGTGACTTCGAAAGTGATAGACTTTGTGGAGAAACTGATAGTGAA
GTGGATGTACGATTCTTCACAGCCTCTTCATTATCTCTCCGGTAACTTCG
CTCCGGTTCCTGACGAGACTCCTCCTACTAAAGACCTCCACGTCATCGGC
CGTCTCCCCGATTGCTTGAATGGGGAGTTTGTCAGGGTTGGTCCTAACCC
CAAGTTTGCCCCTGTGGCCGGATACCACTGGTTTGATGGAGATGGCATGA
TTCATGGTCTGCGCATCAAAGACGGAAAGGCTACATATGTCTCCCGTTTT
GTGAGGACTTCACGTCTTAAACAGGAAGAATTTTTTGGAGGTGCTAAATT
TATGAAGATTGGAGACCTTAAGGGGCTATTTGGATTACTCATGGTTAACA
TGCAAATGCTTAGAGCAAAGTTGAAAGTACTGGATGTTTCATATGGAAAT
GGGACAGCTAATACAGCTCTTGTATATCACCATGGGAAACTTCTAGCACT
CTCAGAGGCGGACAAACCATATGCCGTCAAAGTTCTGGAAGATGGAGATC
TGCAAACACTTGGTATGATTGACTATGACAAAAG
back to top