CF931497

Overview
NameCF931497
Unique NameCF931497
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length568
Libraries
Library NameType
Mature leaf blade cDNA subtraction librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF931497 vs. ExPASy Swiss-Prot
Match: NHP2_ARATH (H/ACA ribonucleoprotein complex subunit 2-like protein OS=Arabidopsis thaliana GN=At5g08180 PE=1 SV=1)

HSP 1 Score: 232.261 bits (591), Expect = 1.305e-60
Identity = 114/155 (73.55%), Postives = 133/155 (85.81%), Query Frame = 3
Query:   66 MGSDSEAERTPHKDEKKKIVSLAPIAKPLAGKKLSKRTLKLVRRAAEHKCLKRGVKEVVKSIRRGHKGLCVIAGNISPIDVITHVPILCEESDVPYIYVASKEDLANAGATKRPTCCVLVLTKPTKGELGQEEQDKLKADYTLVVEDVKELASSL 530
            MGSD+EAE++  K++KK  ++LAPIAKPLAGKKL KRT KL+++AA  KCLKRGVKEVVKSIRRG KGLCVIAGNISPIDVITH+PILCEE+ VPY+YV SKEDLA AGATKRPTCCVLV+ KP KG+L  EE  KLK DY  V +D+KELA+S+
Sbjct:    1 MGSDTEAEKSIQKEKKKYAITLAPIAKPLAGKKLQKRTFKLIQKAAGKKCLKRGVKEVVKSIRRGQKGLCVIAGNISPIDVITHLPILCEEAGVPYVYVPSKEDLAQAGATKRPTCCVLVMLKPAKGDLTAEELAKLKTDYEQVSDDIKELATSV 155          
BLAST of CF931497 vs. ExPASy Swiss-Prot
Match: NHP2_YEAST (H/ACA ribonucleoprotein complex subunit 2 OS=Saccharomyces cerevisiae GN=NHP2 PE=1 SV=2)

HSP 1 Score: 145.976 bits (367), Expect = 1.230e-34
Identity = 74/160 (46.25%), Postives = 112/160 (70.00%), Query Frame = 3
Query:   66 MGSDSEAERTPHKDEKK---------KIVSLAPIAKPLAGKKLSKRTLKLVRRAAEHKCLKRGVKEVVKSIRRGHKGLCVIAGNISPIDVITHVPILCEESDVPYIYVASKEDLANAGATKRPTCCVLVLTKPTKGELGQEEQDKLKADYTLVVEDVKEL 518
            MG D++     HK+ K+         ++ ++ P AKPLA KKL+K+ LK V++A++ K +KRGVKEVVK++R+G KGL VIAG+ISP DVI+H+P+LCE+  VPYI++ SK+DL  AGATKRPT  V ++    K + G+ ++++ K  +  VV++V+ L
Sbjct:    1 MGKDNKE----HKESKESKTVDNYEARMPAVLPFAKPLASKKLNKKVLKTVKKASKAKNVKRGVKEVVKALRKGEKGLVVIAGDISPADVISHIPVLCEDHSVPYIFIPSKQDLGAAGATKRPTSVVFIVPGSNKKKDGKNKEEEYKESFNEVVKEVQAL 156          
BLAST of CF931497 vs. ExPASy Swiss-Prot
Match: NHP2_SCHPO (H/ACA ribonucleoprotein complex subunit 2 OS=Schizosaccharomyces pombe GN=nhp2 PE=1 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 9.745e-32
Identity = 64/131 (48.85%), Postives = 98/131 (74.81%), Query Frame = 3
Query:  126 SLAPIAKPLAGKKLSKRTLKLVRRAAEHKCLKRGVKEVVKSIRRGHKGLCVIAGNISPIDVITHVPILCEESDVPYIYVASKEDLANAGATKRPTCCVLVLTKPTKGELGQEEQDKLKADYTLVVEDVKEL 518
            +L PIAKPLA KKL+K+ +K V++A++ K + RGVKEVVK++R+G KGL ++AG+ISP+DVI+H+P+LCE+++VPY+Y  SKE L  A  TKRPT CV+++    K ++ + E+   K  Y  ++++V  L
Sbjct:   24 ALMPIAKPLAPKKLNKKMMKTVKKASKQKHILRGVKEVVKAVRKGEKGLVILAGDISPMDVISHIPVLCEDNNVPYLYTVSKELLGEASNTKRPTSCVMIVPGGKKKDMSKVEE--YKESYEEIIKEVPAL 152          
BLAST of CF931497 vs. ExPASy Swiss-Prot
Match: NHP2_XENTR (H/ACA ribonucleoprotein complex subunit 2-like protein OS=Xenopus tropicalis GN=nhp2 PE=2 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 5.005e-28
Identity = 58/123 (47.15%), Postives = 81/123 (65.85%), Query Frame = 3
Query:   57 KKKMGSDSEAERTPHKDEKKKIVSLAPIAKPLAGKKLSKRTLKLVRRAAEHKCLKRGVKEVVKSIRRGHKGLCVIAGNISPIDVITHVPILCEESDVPYIYVASKEDLANAGATKRPTCCVLV 425
            K K     E   TP K   + +  L PIAKPLAG+KL+K+  K V++A + K ++RGVKEV K I +G KG+ V+AG+  PI+V  H+P++CE+  +PY YV SK DL  A  +KRPTC +L+
Sbjct:    3 KAKKEESEEVPETPSKSYDELLAYLNPIAKPLAGRKLTKKLYKCVKKAIKQKNIRRGVKEVQKFINKGEKGIVVMAGDTLPIEVYCHIPVMCEDRGIPYSYVPSKSDLGAAAGSKRPTCVILI 125          
BLAST of CF931497 vs. ExPASy Swiss-Prot
Match: NHP2_XENLA (H/ACA ribonucleoprotein complex subunit 2-like protein OS=Xenopus laevis GN=nhp2 PE=2 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 3.244e-27
Identity = 57/123 (46.34%), Postives = 81/123 (65.85%), Query Frame = 3
Query:   57 KKKMGSDSEAERTPHKDEKKKIVSLAPIAKPLAGKKLSKRTLKLVRRAAEHKCLKRGVKEVVKSIRRGHKGLCVIAGNISPIDVITHVPILCEESDVPYIYVASKEDLANAGATKRPTCCVLV 425
            K K     E   TP K   + +  L P+AKPLAG+KL+K+  K V++A + K ++RGVKEV K I +G KG+ V+AG+  PI+V  H+P++CE+  +PY YV SK DL  A  +KRPTC +L+
Sbjct:    3 KVKKEECEEVPETPSKSYDELLSYLNPVAKPLAGRKLTKKLYKCVKKAIKQKNIRRGVKEVQKFINKGEKGIVVMAGDTLPIEVYCHIPVMCEDRGIPYSYVPSKSDLGAAAGSKRPTCVILI 125          
BLAST of CF931497 vs. ExPASy Swiss-Prot
Match: NHP2_DANRE (H/ACA ribonucleoprotein complex subunit 2-like protein OS=Danio rerio GN=nhp2 PE=2 SV=1)

HSP 1 Score: 117.472 bits (293), Expect = 4.684e-26
Identity = 65/154 (42.21%), Postives = 93/154 (60.39%), Query Frame = 3
Query:   57 KKKMGSDSEAERTPHKDEKKKIVSLAPIAKPLAGKKLSKRTLKLVRRAAEHKCLKRGVKEVVKSIRRGHKGLCVIAGNISPIDVITHVPILCEESDVPYIYVASKEDLANAGATKRPTCCVLVLTKPTKGELGQEEQDKLKADYTLVVEDVKEL 518
            KK+   + E   T  K  ++ I ++ PIA PLA +KLSK+  K V++AA+ K ++RGVKEV K I +G  G+ V AG+  PIDV  H+PI+CE+  +PY YV SK DL ++  +KRPTC +++  KP          D+ K  Y   VE+V  L
Sbjct:    5 KKEKAGEEETGGT-EKSYQELIANINPIANPLASRKLSKKLYKCVKKAAKVKQIRRGVKEVQKFINKGETGIVVFAGDTLPIDVYCHLPIMCEDRSLPYAYVPSKVDLGSSAGSKRPTCVIMI--KP---------HDEYKEAYDECVEEVTSL 146          
BLAST of CF931497 vs. ExPASy Swiss-Prot
Match: NHP2_CAEBR (Putative H/ACA ribonucleoprotein complex subunit 2-like protein OS=Caenorhabditis briggsae GN=CBG18231 PE=3 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 7.990e-26
Identity = 54/125 (43.20%), Postives = 87/125 (69.60%), Query Frame = 3
Query:   72 SDSEAERTPHKDEKKKIVSLA-PIAKPLAGKKLSKRTLKLVRRAAE-HKCLKRGVKEVVKSIRRGHKGLCVIAGNISPIDVITHVPILCEESDVPYIYVASKEDLANAGATKRPTCCVLVLTKPT 440
            ++  A  T  KDE + +  L  PIA+PLA +KL+K+  KL+++AA   K L+ G+K+V K +R+  KG+C++AGN+SPIDV +H+P +CEE ++PY+Y+ S+E L  A   +RP+  +L+  KP+
Sbjct:   19 AEDVAALTTEKDEYQALCELVNPIAQPLANRKLAKKVYKLIKKAAAGEKTLREGIKDVQKELRKNEKGICILAGNVSPIDVYSHIPAICEEKEIPYVYIPSREQLGLAVGHRRPS--ILIFVKPS 141          
BLAST of CF931497 vs. ExPASy Swiss-Prot
Match: NHP2_MOUSE (H/ACA ribonucleoprotein complex subunit 2 OS=Mus musculus GN=Nhp2 PE=2 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.044e-25
Identity = 60/141 (42.55%), Postives = 94/141 (66.67%), Query Frame = 3
Query:   60 KKMGSDSEAERTPHKDE---KKKIVSLAPIAKPLAGKKLSKRTLKLVRRAAEHKCLKRGVKEVVKSIRRGHKGLCVIAGNISPIDVITHVPILCEESDVPYIYVASKEDLANAGATKRPTCCVLVLTKPTKGELGQEEQDK 473
            K    +SEA+     +E   K+ +V+L PIA+PLA ++L+++  K +++A + K ++RGVKEV K + +G KG+ V+AG+  PI+V  H+P+LCE+ ++PY+Y+ SK DL  A  +KRPTC ++V  KP   E  QE  DK
Sbjct:    5 KAAPEESEAQAEGCSEERTYKELLVNLNPIAQPLASRRLTRKLYKCIKKAVKQKQIRRGVKEVQKFVNKGEKGIMVLAGDTLPIEVYCHLPVLCEDQNLPYVYIPSKTDLGAATGSKRPTCVIMV--KP--HEEYQETYDK 141          
BLAST of CF931497 vs. ExPASy Swiss-Prot
Match: NHP2_CAEEL (Putative H/ACA ribonucleoprotein complex subunit 2-like protein OS=Caenorhabditis elegans GN=Y48A6B.3 PE=2 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 2.325e-25
Identity = 54/118 (45.76%), Postives = 84/118 (71.19%), Query Frame = 3
Query:   93 TPHKDEKKKIVSLA-PIAKPLAGKKLSKRTLKLVRRA-AEHKCLKRGVKEVVKSIRRGHKGLCVIAGNISPIDVITHVPILCEESDVPYIYVASKEDLANAGATKRPTCCVLVLTKPT 440
            T  KDE + +  L  PIA+PLA +KL+K+  KL+++A A  K L+ G+K+V K +RR  KG+C++AGN+SPIDV +H+P +CEE ++PY+Y+ S+E L  A   +RP+  +L+  KP+
Sbjct:   26 TTEKDEYQALCELVNPIAQPLANRKLAKKVYKLIKKASAGDKTLREGIKDVQKELRRNEKGICILAGNVSPIDVYSHIPGICEEKEIPYVYIPSREQLGLAVGHRRPS--ILIFVKPS 141          
BLAST of CF931497 vs. ExPASy Swiss-Prot
Match: NHP2_BRABE (H/ACA ribonucleoprotein complex subunit 2-like protein OS=Branchiostoma belcheri PE=2 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 2.325e-25
Identity = 58/150 (38.67%), Postives = 96/150 (64.00%), Query Frame = 3
Query:   42 EREN*KKKMGSDSEAERTPHKDEKKKIVSLAPIAKPLAGKKLSKRTLKLVRRAAEHKCLKRGVKEVVKSIRRGHKGLCVIAGNISPIDVITHVPILCEESDVPYIYVASKEDLANAGATKRPTCCVLVLTKPTKGELGQEEQDKLKADYT 491
            E+E  ++  G+  + ER      +     + PIA PLA +KL+KR  K V++A++ K +++G+++V K I++G +G+ +IAG+ +PI+V  H+P++CE++ +PY YV +K+DL  A  +KRPTCCVL+  KP   E  Q   D+   D T
Sbjct:   12 EKEEHEESGGNKEDRERA----YEVLATRVNPIASPLAARKLTKRLYKTVKKASKEKNIRKGIRDVQKFIKKGERGIVLIAGDTTPIEVYCHLPVVCEDAKIPYCYVPAKQDLGEAAGSKRPTCCVLL--KP--NESYQSSYDECLTDVT 153          
The following BLAST results are available for this feature:
BLAST of CF931497 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 62
Match NameE-valueIdentityDescription
NHP2_ARATH1.305e-6073.55H/ACA ribonucleoprotein complex subunit 2-like pro... [more]
NHP2_YEAST1.230e-3446.25H/ACA ribonucleoprotein complex subunit 2 OS=Sacch... [more]
NHP2_SCHPO9.745e-3248.85H/ACA ribonucleoprotein complex subunit 2 OS=Schiz... [more]
NHP2_XENTR5.005e-2847.15H/ACA ribonucleoprotein complex subunit 2-like pro... [more]
NHP2_XENLA3.244e-2746.34H/ACA ribonucleoprotein complex subunit 2-like pro... [more]
NHP2_DANRE4.684e-2642.21H/ACA ribonucleoprotein complex subunit 2-like pro... [more]
NHP2_CAEBR7.990e-2643.20Putative H/ACA ribonucleoprotein complex subunit 2... [more]
NHP2_MOUSE1.044e-2542.55H/ACA ribonucleoprotein complex subunit 2 OS=Mus m... [more]
NHP2_CAEEL2.325e-2545.76Putative H/ACA ribonucleoprotein complex subunit 2... [more]
NHP2_BRABE2.325e-2538.67H/ACA ribonucleoprotein complex subunit 2-like pro... [more]

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Properties
Property NameValue
Genbank descriptionEST0568 Mature leaf blade cDNA subtraction library Citrus sinensis cDNA clone 24LB259 similar to nhp2-like protein (acc# NP_196435), mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF931497 ID=CF931497; Name=CF931497; organism=Citrus sinensis; type=EST; length=568bp
CTGAAAAAGAAAAACCCTAGCTCTGTTTCGTGCAATTCATTGAGAGAGAG
AACTGAAAGAAGAAAATGGGAAGCGACAGCGAAGCAGAGAGAACCCCACA
TAAAGATGAGAAGAAGAAGATTGTTAGTCTTGCTCCCATTGCTAAACCTC
TAGCTGGCAAGAAGCTCTCCAAAAGAACCCTCAAGCTTGTCCGTCGAGCT
GCTGAACACAAATGCTTGAAGAGAGGAGTGAAGGAAGTGGTCAAAAGTAT
CCGCCGTGGTCATAAAGGATTATGTGTTATAGCTGGGAACATTAGTCCAA
TTGACGTCATCACTCACGTTCCTATCTTATGTGAAGAGTCTGATGTTCCC
TACATTTATGTTGCTTCAAAAGAGGATCTTGCAAATGCAGGTGCCACAAA
GAGGCCAACATGCTGTGTTTTGGTGCTAACTAAGCCAACTAAGGGAGAAC
TAGGACAAGAGGAACAAGATAAGTTGAAAGCTGATTATACCCTCGTTGTA
GAGGATGTGAAGGAACTGGCATCCTCTCTTTTCTGATGAACTAGACTTAA
AGCCAACAAAGATGATTG
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