CK740014

Overview
NameCK740014
Unique NameCK740014
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length264
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK740014 vs. ExPASy Swiss-Prot
Match: GLYM_ARATH (Serine hydroxymethyltransferase, mitochondrial OS=Arabidopsis thaliana GN=SHM1 PE=1 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 1.757e-14
Identity = 29/56 (51.79%), Postives = 39/56 (69.64%), Query Frame = 1 
Query:   97 RDTALSPGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGG 264
            R   L  GGT+NHLVL +L+P G+ G++VEK+ +  +I  NKN V GD SA+ PGG
Sbjct:  373 RGYELVSGGTDNHLVLVNLKPKGIDGSRVEKVLEAVHIASNKNTVPGDVSAMVPGG 428

HSP 2 Score: 37.7354 bits (86), Expect = 1.757e-14
Identity = 18/38 (47.37%), Postives = 25/38 (65.79%), Query Frame = 3 
Query:    3 VALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVT 116
            VALKQA+T  +KAY +QV +N+      L  +GY LV+
Sbjct:  342 VALKQATTSEYKAYQEQVLSNSAKFAQTLMERGYELVS 379
[Close]
BLAST of CK740014 vs. ExPASy Swiss-Prot
Match: GLYM_FLAPR (Serine hydroxymethyltransferase 1, mitochondrial OS=Flaveria pringlei PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 2.966e-14
Identity = 29/52 (55.77%), Postives = 37/52 (71.15%), Query Frame = 1 
Query:  109 LSPGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGG 264
            L  GGTENHLVL +L+  G+ G+KVEK+ +  +I  NKN V GD SA+ PGG
Sbjct:  378 LVSGGTENHLVLVNLKNKGIDGSKVEKVLEAVHIAANKNTVPGDVSAMVPGG 429

HSP 2 Score: 37.7354 bits (86), Expect = 2.966e-14
Identity = 18/38 (47.37%), Postives = 24/38 (63.16%), Query Frame = 3 
Query:    3 VALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVT 116
            VALKQA+T  +KAY +QV +N+      L   GY LV+
Sbjct:  343 VALKQATTAEYKAYQEQVMSNSAKFAETLVKSGYELVS 380
[Close]
BLAST of CK740014 vs. ExPASy Swiss-Prot
Match: GLYC_RABIT (Serine hydroxymethyltransferase, cytosolic OS=Oryctolagus cuniculus GN=SHMT1 PE=1 SV=2)

HSP 1 Score: 55.8398 bits (133), Expect = 2.971e-14
Identity = 25/49 (51.02%), Postives = 32/49 (65.31%), Query Frame = 1 
Query:  118 GGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGG 264
            GG++NHL+L DLR  G  G + EK+ + C+I  NKN   GD SAL P G
Sbjct:  352 GGSDNHLILVDLRSKGTDGGRAEKVLEACSIACNKNTCPGDKSALRPSG 400

HSP 2 Score: 41.5874 bits (96), Expect = 2.971e-14
Identity = 22/38 (57.89%), Postives = 24/38 (63.16%), Query Frame = 3 
Query:    3 VALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVT 116
            VALKQA TP FK Y +QV AN  AL   L   GY +VT
Sbjct:  314 VALKQAMTPEFKEYQRQVVANCRALSAALVELGYKIVT 351
[Close]
BLAST of CK740014 vs. ExPASy Swiss-Prot
Match: GLYM_ASHGO (Serine hydroxymethyltransferase, mitochondrial OS=Ashbya gossypii GN=SHM1 PE=3 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 3.858e-14
Identity = 26/56 (46.43%), Postives = 37/56 (66.07%), Query Frame = 1 
Query:   97 RDTALSPGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGG 264
            R  +L  GGT+ HL+L DL P+G+ G+++E + +  NI  NKN + GD SAL P G
Sbjct:  359 RGFSLVSGGTDTHLLLIDLSPMGIDGSRLETILERLNIAANKNTIPGDKSALYPSG 414

HSP 2 Score: 39.6614 bits (91), Expect = 3.858e-14
Identity = 20/38 (52.63%), Postives = 25/38 (65.79%), Query Frame = 3 
Query:    3 VALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVT 116
            VALKQA+TP FK Y   V  NA   G  L+ +G+SLV+
Sbjct:  328 VALKQAATPEFKNYQTAVVENAKVFGEELSKRGFSLVS 365
[Close]
BLAST of CK740014 vs. ExPASy Swiss-Prot
Match: GLYC_MOUSE (Serine hydroxymethyltransferase, cytosolic OS=Mus musculus GN=Shmt1 PE=1 SV=2)

HSP 1 Score: 56.6102 bits (135), Expect = 5.016e-14
Identity = 25/49 (51.02%), Postives = 32/49 (65.31%), Query Frame = 1 
Query:  118 GGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGG 264
            GG++NHL+L DLR  G  G + EK+ + C+I  NKN   GD SAL P G
Sbjct:  346 GGSDNHLILMDLRSKGTDGGRAEKVLEACSIACNKNTCPGDKSALRPSG 394

HSP 2 Score: 40.0466 bits (92), Expect = 5.016e-14
Identity = 22/38 (57.89%), Postives = 24/38 (63.16%), Query Frame = 3 
Query:    3 VALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVT 116
            VALKQA T  FK Y  QV AN  AL + LT  GY +VT
Sbjct:  308 VALKQAMTTEFKIYQLQVLANCRALSDALTELGYKIVT 345
[Close]
BLAST of CK740014 vs. ExPASy Swiss-Prot
Match: GLYM_SOLTU (Serine hydroxymethyltransferase, mitochondrial OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 6.504e-14
Identity = 26/52 (50.00%), Postives = 37/52 (71.15%), Query Frame = 1 
Query:  109 LSPGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGG 264
            L  GGT+NHLVL +++  G+ G++VEK+ +  +I  NKN V GD SA+ PGG
Sbjct:  378 LVSGGTDNHLVLVNMKNKGIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGG 429

HSP 2 Score: 39.6614 bits (91), Expect = 6.504e-14
Identity = 19/38 (50.00%), Postives = 26/38 (68.42%), Query Frame = 3 
Query:    3 VALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVT 116
            VALKQA+TP ++AY +QV +N+      L  KGY LV+
Sbjct:  343 VALKQATTPEYRAYQEQVLSNSSKFAQALGEKGYELVS 380
[Close]
BLAST of CK740014 vs. ExPASy Swiss-Prot
Match: GLYN_FLAPR (Serine hydroxymethyltransferase 2, mitochondrial OS=Flaveria pringlei PE=2 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 8.448e-14
Identity = 28/52 (53.85%), Postives = 37/52 (71.15%), Query Frame = 1 
Query:  109 LSPGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGG 264
            L  GGTENHLVL +L+  G+ G++VEK+ +  +I  NKN V GD SA+ PGG
Sbjct:  378 LVSGGTENHLVLVNLKNKGIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGG 429

HSP 2 Score: 37.3502 bits (85), Expect = 8.448e-14
Identity = 18/38 (47.37%), Postives = 23/38 (60.53%), Query Frame = 3 
Query:    3 VALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVT 116
            VALKQA+T  +KAY +QV +N       L   GY LV+
Sbjct:  343 VALKQATTAEYKAYQEQVMSNCAKFAETLVKSGYELVS 380
[Close]
BLAST of CK740014 vs. ExPASy Swiss-Prot
Match: GLYC_CANAL (Serine hydroxymethyltransferase, cytosolic OS=Candida albicans GN=SHM2 PE=1 SV=3)

HSP 1 Score: 52.7582 bits (125), Expect = 8.469e-14
Identity = 23/48 (47.92%), Postives = 34/48 (70.83%), Query Frame = 1 
Query:  121 GTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGG 264
            GT++H+VL  L+   + G +VE +C+  NI +NKN++ GD SAL PGG
Sbjct:  345 GTDSHMVLVSLKDKQIDGARVETVCEKINIALNKNSIPGDKSALVPGG 392

HSP 2 Score: 43.1282 bits (100), Expect = 8.469e-14
Identity = 22/37 (59.46%), Postives = 26/37 (70.27%), Query Frame = 3 
Query:    6 ALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVT 116
            ALKQA+TP FK Y +QV  NA AL +  T KGY LV+
Sbjct:  307 ALKQANTPEFKEYQEQVLKNAKALESEFTKKGYKLVS 343
[Close]
BLAST of CK740014 vs. ExPASy Swiss-Prot
Match: GLYC_CANGA (Serine hydroxymethyltransferase, cytosolic OS=Candida glabrata GN=SHM2 PE=3 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 1.100e-13
Identity = 26/52 (50.00%), Postives = 36/52 (69.23%), Query Frame = 1 
Query:  109 LSPGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGG 264
            L   GT++H+VL  LR  G+ G +VE +C+  NI +NKN++ GD SAL PGG
Sbjct:  340 LVSNGTDSHMVLVSLREKGVDGARVEYVCEKINIALNKNSIPGDKSALVPGG 391

HSP 2 Score: 38.891 bits (89), Expect = 1.100e-13
Identity = 21/37 (56.76%), Postives = 23/37 (62.16%), Query Frame = 3 
Query:    6 ALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVT 116
            ALKQA+TP FK Y  QV  NA AL N     GY LV+
Sbjct:  306 ALKQAATPEFKEYQTQVLKNAKALENEFQTLGYRLVS 342
[Close]
BLAST of CK740014 vs. ExPASy Swiss-Prot
Match: GLYC_PONAB (Serine hydroxymethyltransferase, cytosolic OS=Pongo abelii GN=SHMT1 PE=2 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.428e-13
Identity = 25/49 (51.02%), Postives = 32/49 (65.31%), Query Frame = 1 
Query:  118 GGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGG 264
            GG++NHL+L DLR  G  G + EK+ + C+I  NKN   GD SAL P G
Sbjct:  352 GGSDNHLILVDLRSKGTDGGRAEKVLEACSIACNKNTCPGDRSALRPSG 400

HSP 2 Score: 39.6614 bits (91), Expect = 1.428e-13
Identity = 22/38 (57.89%), Postives = 23/38 (60.53%), Query Frame = 3 
Query:    3 VALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVT 116
            VALKQA T  FK Y  QV AN  AL   LT  GY +VT
Sbjct:  314 VALKQAMTLEFKVYQHQVVANCRALSEALTELGYKIVT 351
The following BLAST results are available for this feature:
BLAST of CK740014 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 33
Match NameE-valueIdentityDescription
GLYM_ARATH1.757e-1451.79Serine hydroxymethyltransferase, mitochondrial OS=... [more]
GLYM_FLAPR2.966e-1455.77Serine hydroxymethyltransferase 1, mitochondrial O... [more]
GLYC_RABIT2.971e-1451.02Serine hydroxymethyltransferase, cytosolic OS=Oryc... [more]
GLYM_ASHGO3.858e-1446.43Serine hydroxymethyltransferase, mitochondrial OS=... [more]
GLYC_MOUSE5.016e-1451.02Serine hydroxymethyltransferase, cytosolic OS=Mus ... [more]
GLYM_SOLTU6.504e-1450.00Serine hydroxymethyltransferase, mitochondrial OS=... [more]
GLYN_FLAPR8.448e-1453.85Serine hydroxymethyltransferase 2, mitochondrial O... [more]
GLYC_CANAL8.469e-1447.92Serine hydroxymethyltransferase, cytosolic OS=Cand... [more]
GLYC_CANGA1.100e-1350.00Serine hydroxymethyltransferase, cytosolic OS=Cand... [more]
GLYC_PONAB1.428e-1351.02Serine hydroxymethyltransferase, cytosolic OS=Pong... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_6508 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE64D06 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK740014 ID=CK740014; Name=CK740014; organism=Citrus sinensis; type=EST; length=264bp
CTGTTGCCCTGAAACAGGCCTCGACCCCTGCATTCAAGGCCTATGCGAAA
CAAGTCAAGGCCAATGCAGTTGCACTTGGGAACTACCTGACGGGCAAGGG
ATACAGCCTTGTCACCTGGTGGAACTGAGAACCACCTTGTTCTCTGGGAT
CTACGCCCTCTTGGATTGACTGGCAATAAGGTTGAGAAGCTCTGTGACCT
TTGCAACATTACTGTGAACAAGAATGCTGTGTTTGGTGACAGCAGTGCCT
TGGCCCCTGGAGGA
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