CK740014

Overview
NameCK740014
Unique NameCK740014
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length264
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig5829 contig Csv1_Contig5829:1000..1261. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CK740014 vs. ExPASy Swiss-Prot
Match: GLYC_HUMAN (Serine hydroxymethyltransferase, cytosolic OS=Homo sapiens GN=SHMT1 PE=1 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.428e-13
Identity = 25/49 (51.02%), Postives = 32/49 (65.31%), Query Frame = 1 
Query:  118 GGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGG 264
            GG++NHL+L DLR  G  G + EK+ + C+I  NKN   GD SAL P G
Sbjct:  352 GGSDNHLILVDLRSKGTDGGRAEKVLEACSIACNKNTCPGDRSALRPSG 400

HSP 2 Score: 39.6614 bits (91), Expect = 1.428e-13
Identity = 22/38 (57.89%), Postives = 23/38 (60.53%), Query Frame = 3 
Query:    3 VALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVT 116
            VALKQA T  FK Y  QV AN  AL   LT  GY +VT
Sbjct:  314 VALKQAMTLEFKVYQHQVVANCRALSEALTELGYKIVT 351
[Close]
BLAST of CK740014 vs. ExPASy Swiss-Prot
Match: GLYM_CANGA (Serine hydroxymethyltransferase, mitochondrial OS=Candida glabrata GN=SHM1 PE=3 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 1.855e-13
Identity = 25/56 (44.64%), Postives = 35/56 (62.50%), Query Frame = 1 
Query:   97 RDTALSPGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGG 264
            R   L  GGT+NHL+L +L  +G+ G ++E + +  NI  NKN + GD SAL P G
Sbjct:  347 RGFELVSGGTDNHLLLLNLSNMGIDGARLEAILEKINIAANKNTIPGDKSALFPSG 402

HSP 2 Score: 41.9726 bits (97), Expect = 1.855e-13
Identity = 21/38 (55.26%), Postives = 26/38 (68.42%), Query Frame = 3 
Query:    3 VALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVT 116
            VALKQA TP F  Y KQV +NA A G+ L  +G+ LV+
Sbjct:  316 VALKQAKTPEFVEYQKQVVSNAKAFGDELLKRGFELVS 353
[Close]
BLAST of CK740014 vs. ExPASy Swiss-Prot
Match: GLYC_YEAST (Serine hydroxymethyltransferase, cytosolic OS=Saccharomyces cerevisiae GN=SHM2 PE=1 SV=2)

HSP 1 Score: 56.9954 bits (136), Expect = 1.856e-13
Identity = 26/52 (50.00%), Postives = 36/52 (69.23%), Query Frame = 1 
Query:  109 LSPGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGG 264
            L   GT++H+VL  LR  G+ G +VE +C+  NI +NKN++ GD SAL PGG
Sbjct:  340 LVSNGTDSHMVLVSLREKGVDGARVEYICEKINIALNKNSIPGDKSALVPGG 391

HSP 2 Score: 37.7354 bits (86), Expect = 1.856e-13
Identity = 20/37 (54.05%), Postives = 23/37 (62.16%), Query Frame = 3 
Query:    6 ALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVT 116
            ALKQA+TP FK Y  QV  NA AL +     GY LV+
Sbjct:  306 ALKQAATPEFKEYQTQVLKNAKALESEFKNLGYRLVS 342
[Close]
BLAST of CK740014 vs. ExPASy Swiss-Prot
Match: GLYC_CAEBR (Serine hydroxymethyltransferase OS=Caenorhabditis briggsae GN=mel-32 PE=3 SV=3)

HSP 1 Score: 62.7734 bits (151), Expect = 2.406e-13
Identity = 29/53 (54.72%), Postives = 38/53 (71.70%), Query Frame = 1 
Query:  106 ALSPGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGG 264
            +L+ GGT+NHL+L DLRP+G+ G + E + DL +I  NKN   GD SAL PGG
Sbjct:  377 SLATGGTDNHLLLVDLRPIGVEGARAEHILDLAHIACNKNTCPGDVSALRPGG 429

HSP 2 Score: 31.5722 bits (70), Expect = 2.406e-13
Identity = 17/38 (44.74%), Postives = 20/38 (52.63%), Query Frame = 3 
Query:    3 VALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVT 116
            VALKQ  +  F  Y +Q+  NA  L   L   GYSL T
Sbjct:  343 VALKQCLSEDFVQYGEQILKNAKTLAERLKKHGYSLAT 380
[Close]
BLAST of CK740014 vs. ExPASy Swiss-Prot
Match: GLYC_SCHPO (Probable serine hydroxymethyltransferase, cytosolic OS=Schizosaccharomyces pombe GN=shm2 PE=2 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 2.411e-13
Identity = 29/56 (51.79%), Postives = 36/56 (64.29%), Query Frame = 1 
Query:   97 RDTALSPGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGG 264
            R   L  GGT+ HLVL DL   G+ G +VE++ +L NI+ NKN V GD SAL P G
Sbjct:  336 RGYKLVSGGTDTHLVLVDLTDKGVDGARVERILELVNISANKNTVPGDKSALIPRG 391

HSP 2 Score: 37.7354 bits (86), Expect = 2.411e-13
Identity = 19/38 (50.00%), Postives = 23/38 (60.53%), Query Frame = 3 
Query:    3 VALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVT 116
            VAL QA TP F  Y K V +NA A+ N    +GY LV+
Sbjct:  305 VALGQAKTPEFYQYQKDVLSNAKAMANAFITRGYKLVS 342
[Close]
BLAST of CK740014 vs. ExPASy Swiss-Prot
Match: GLYA_HELMI (Serine hydroxymethyltransferase OS=Heliobacterium modesticaldum (strain ATCC 51547 / Ice1) GN=glyA PE=3 SV=1)

HSP 1 Score: 51.6026 bits (122), Expect = 2.419e-13
Identity = 26/45 (57.78%), Postives = 30/45 (66.67%), Query Frame = 1 
Query:   97 RDTALSPGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAV 231
            R   L  GGT+NHL+L DLR   LTG + EK  D C ITVNKNA+
Sbjct:  302 RGFQLVSGGTDNHLMLVDLRNKQLTGKEAEKRLDECRITVNKNAI 346

HSP 2 Score: 42.743 bits (99), Expect = 2.419e-13
Identity = 20/38 (52.63%), Postives = 26/38 (68.42%), Query Frame = 3 
Query:    3 VALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVT 116
            VA ++A  PAF AY KQ+ ANA AL   LT +G+ LV+
Sbjct:  271 VAFQEAMAPAFTAYQKQIAANAAALAKGLTDRGFQLVS 308
[Close]
BLAST of CK740014 vs. ExPASy Swiss-Prot
Match: GLYC_CAEEL (Serine hydroxymethyltransferase OS=Caenorhabditis elegans GN=mel-32 PE=1 SV=2)

HSP 1 Score: 63.5438 bits (153), Expect = 8.891e-13
Identity = 30/53 (56.60%), Postives = 38/53 (71.70%), Query Frame = 1 
Query:  106 ALSPGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGG 264
            AL+ GGT+NHL+L DLRP+G+ G + E + DL +I  NKN   GD SAL PGG
Sbjct:  373 ALATGGTDNHLLLVDLRPIGVEGARAEHVLDLAHIACNKNTCPGDVSALRPGG 425

HSP 2 Score: 28.8758 bits (63), Expect = 8.891e-13
Identity = 15/38 (39.47%), Postives = 20/38 (52.63%), Query Frame = 3 
Query:    3 VALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVT 116
            VAL+Q  +  F  Y +QV  NA  L   +   GY+L T
Sbjct:  339 VALRQCLSEDFVQYGEQVLKNAKTLAERMKKHGYALAT 376
[Close]
BLAST of CK740014 vs. ExPASy Swiss-Prot
Match: GLYA_SYMTH (Serine hydroxymethyltransferase OS=Symbiobacterium thermophilum GN=glyA PE=3 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 8.940e-13
Identity = 27/48 (56.25%), Postives = 32/48 (66.67%), Query Frame = 1 
Query:   97 RDTALSPGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGD 240
            R   L  GGT+NHL+L DLRP G+TG   E L D  +ITVNKNA+  D
Sbjct:  299 RGYRLVTGGTDNHLMLVDLRPKGITGRDAEHLLDRVSITVNKNAIPND 346

HSP 2 Score: 38.1206 bits (87), Expect = 8.940e-13
Identity = 20/38 (52.63%), Postives = 23/38 (60.53%), Query Frame = 3 
Query:    3 VALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVT 116
            VA KQ S P ++AY  QV  NA AL   L  +GY LVT
Sbjct:  268 VAFKQLSDPDYRAYCGQVVKNAKALAQALLERGYRLVT 305
[Close]
BLAST of CK740014 vs. ExPASy Swiss-Prot
Match: GLYM_KLULA (Serine hydroxymethyltransferase, mitochondrial OS=Kluyveromyces lactis GN=SHM1 PE=3 SV=1)

HSP 1 Score: 51.6026 bits (122), Expect = 4.265e-12
Identity = 25/52 (48.08%), Postives = 33/52 (63.46%), Query Frame = 1 
Query:  109 LSPGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGG 264
            L  GGT+ HL+L +L  LG+ G ++E L +  NI  NKN + GD SAL P G
Sbjct:  364 LVSGGTDTHLILINLSNLGIDGARLETLLENINIAANKNTIPGDKSALFPSG 415

HSP 2 Score: 38.5058 bits (88), Expect = 4.265e-12
Identity = 20/38 (52.63%), Postives = 23/38 (60.53%), Query Frame = 3 
Query:    3 VALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVT 116
            VALKQA+TP FK Y   V  NA   G  L  KG+ LV+
Sbjct:  329 VALKQAATPEFKEYQAAVVENARIFGEELVKKGFELVS 366
[Close]
BLAST of CK740014 vs. ExPASy Swiss-Prot
Match: GLYA_DICT6 (Serine hydroxymethyltransferase OS=Dictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12) GN=glyA PE=3 SV=1)

HSP 1 Score: 49.2914 bits (116), Expect = 3.459e-11
Identity = 23/41 (56.10%), Postives = 29/41 (70.73%), Query Frame = 1 
Query:  109 LSPGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAV 231
            L  GGT+NHL+L DLR  G+TG + EK  +   ITVNKNA+
Sbjct:  303 LVSGGTDNHLMLVDLRDKGITGKEAEKALEEAGITVNKNAI 343

HSP 2 Score: 37.7354 bits (86), Expect = 3.459e-11
Identity = 19/38 (50.00%), Postives = 23/38 (60.53%), Query Frame = 3 
Query:    3 VALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVT 116
            VA K+A TP FK Y KQ+  NA A+   L   GY LV+
Sbjct:  268 VAFKEAMTPEFKEYQKQIILNAKAMAEELMRLGYRLVS 305
The following BLAST results are available for this feature:
BLAST of CK740014 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 33
Match NameE-valueIdentityDescription
GLYC_HUMAN1.428e-1351.02Serine hydroxymethyltransferase, cytosolic OS=Homo... [more]
GLYM_CANGA1.855e-1344.64Serine hydroxymethyltransferase, mitochondrial OS=... [more]
GLYC_YEAST1.856e-1350.00Serine hydroxymethyltransferase, cytosolic OS=Sacc... [more]
GLYC_CAEBR2.406e-1354.72Serine hydroxymethyltransferase OS=Caenorhabditis ... [more]
GLYC_SCHPO2.411e-1351.79Probable serine hydroxymethyltransferase, cytosoli... [more]
GLYA_HELMI2.419e-1357.78Serine hydroxymethyltransferase OS=Heliobacterium ... [more]
GLYC_CAEEL8.891e-1356.60Serine hydroxymethyltransferase OS=Caenorhabditis ... [more]
GLYA_SYMTH8.940e-1356.25Serine hydroxymethyltransferase OS=Symbiobacterium... [more]
GLYM_KLULA4.265e-1248.08Serine hydroxymethyltransferase, mitochondrial OS=... [more]
GLYA_DICT63.459e-1156.10Serine hydroxymethyltransferase OS=Dictyoglomus th... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_6508 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE64D06 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK740014 ID=CK740014; Name=CK740014; organism=Citrus sinensis; type=EST; length=264bp
CTGTTGCCCTGAAACAGGCCTCGACCCCTGCATTCAAGGCCTATGCGAAA
CAAGTCAAGGCCAATGCAGTTGCACTTGGGAACTACCTGACGGGCAAGGG
ATACAGCCTTGTCACCTGGTGGAACTGAGAACCACCTTGTTCTCTGGGAT
CTACGCCCTCTTGGATTGACTGGCAATAAGGTTGAGAAGCTCTGTGACCT
TTGCAACATTACTGTGAACAAGAATGCTGTGTTTGGTGACAGCAGTGCCT
TGGCCCCTGGAGGA
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