CK932947

Overview
NameCK932947
Unique NameCK932947
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length735
Libraries
Library NameType
Developing fruit juice sac at 38 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CK932947 vs. ExPASy Swiss-Prot
Match: FPPS1_LUPAL (Farnesyl pyrophosphate synthetase 1 OS=Lupinus albus GN=FPS1 PE=2 SV=1)

HSP 1 Score: 192.586 bits (488), Expect = 1.878e-48
Identity = 89/100 (89.00%), Postives = 92/100 (92.00%), Query Frame = 2
Query:  434 GKLNRGLSVVDSYRLLKEGKELTDDEFFLSSALGWCIEWLQAYFLVLDDIMDGSHTRRGQPCWFRVPKVGMIAANDGVLLRNHISRILKNHFRDKPYYVD 733
            GKLNRGLSV+DSYRLLK+G EL DDE FL+SALGWCIEWLQAYFLVLDDIMD SHTRRGQPCWFRVPKVGMIAANDGVLLRNHI RILK HFR KPYY D
Sbjct:   46 GKLNRGLSVIDSYRLLKDGHELNDDEIFLASALGWCIEWLQAYFLVLDDIMDNSHTRRGQPCWFRVPKVGMIAANDGVLLRNHIPRILKKHFRGKPYYAD 145          

HSP 2 Score: 78.9518 bits (193), Expect = 3.026e-14
Identity = 35/46 (76.09%), Postives = 40/46 (86.96%), Query Frame = 2
Query:  128 MSDLKSRFIEVYGVLKQELLNDPAFEFDHDSRQWVDRMLDYNVPGG 265
            M+DL+S F+ VY VLK ELL+DPAFEF  DSRQW+DRMLDYNVPGG
Sbjct:    1 MADLRSTFLNVYSVLKSELLHDPAFEFSPDSRQWLDRMLDYNVPGG 46          
BLAST of CK932947 vs. ExPASy Swiss-Prot
Match: FPPS_HELAN (Farnesyl pyrophosphate synthetase OS=Helianthus annuus GN=FPS1 PE=2 SV=1)

HSP 1 Score: 188.348 bits (477), Expect = 3.542e-47
Identity = 88/100 (88.00%), Postives = 94/100 (94.00%), Query Frame = 2
Query:  434 GKLNRGLSVVDSYRLLKEGKELTDDEFFLSSALGWCIEWLQAYFLVLDDIMDGSHTRRGQPCWFRVPKVGMIAANDGVLLRNHISRILKNHFRDKPYYVD 733
            GKLNRGLSVVDSY+LLK G ELTDDE FL+SALGWCIEWLQAYFLVLDDIMDGSHTRRGQPCWFR+PKVGMIAANDG++LRNH+ RILK HFR KPYYVD
Sbjct:   47 GKLNRGLSVVDSYQLLK-GAELTDDEIFLASALGWCIEWLQAYFLVLDDIMDGSHTRRGQPCWFRLPKVGMIAANDGLILRNHVPRILKKHFRGKPYYVD 145          

HSP 2 Score: 83.1889 bits (204), Expect = 1.605e-15
Identity = 36/45 (80.00%), Postives = 40/45 (88.89%), Query Frame = 2
Query:  131 SDLKSRFIEVYGVLKQELLNDPAFEFDHDSRQWVDRMLDYNVPGG 265
            SDLKS+F+ VY  LK ELLNDPAFEF HDSRQW+D+MLDYNVPGG
Sbjct:    3 SDLKSKFLHVYQTLKSELLNDPAFEFHHDSRQWIDKMLDYNVPGG 47          
BLAST of CK932947 vs. ExPASy Swiss-Prot
Match: FPPS2_LUPAL (Farnesyl pyrophosphate synthetase 2 OS=Lupinus albus GN=FPS2 PE=2 SV=1)

HSP 1 Score: 187.193 bits (474), Expect = 7.890e-47
Identity = 85/100 (85.00%), Postives = 92/100 (92.00%), Query Frame = 2
Query:  434 GKLNRGLSVVDSYRLLKEGKELTDDEFFLSSALGWCIEWLQAYFLVLDDIMDGSHTRRGQPCWFRVPKVGMIAANDGVLLRNHISRILKNHFRDKPYYVD 733
            GKLNRGLSV+DSY+LLK+G+EL D+E FL+SALGWCIEWLQAYFLVLDDIMD SHTRRG PCWFRVPKVGMIA NDGV+LRNHI RILK HFR KPYYVD
Sbjct:   46 GKLNRGLSVIDSYKLLKDGQELNDEEIFLASALGWCIEWLQAYFLVLDDIMDNSHTRRGHPCWFRVPKVGMIAPNDGVVLRNHIPRILKKHFRGKPYYVD 145          

HSP 2 Score: 76.2554 bits (186), Expect = 1.962e-13
Identity = 34/46 (73.91%), Postives = 38/46 (82.61%), Query Frame = 2
Query:  128 MSDLKSRFIEVYGVLKQELLNDPAFEFDHDSRQWVDRMLDYNVPGG 265
            M+D KS F+ VY +LK ELL DPAFEF  DSRQWV+RMLDYNVPGG
Sbjct:    1 MADPKSSFLNVYSILKSELLQDPAFEFSTDSRQWVERMLDYNVPGG 46          
BLAST of CK932947 vs. ExPASy Swiss-Prot
Match: FPPS_ARTAN (Farnesyl pyrophosphate synthetase OS=Artemisia annua GN=FPS1 PE=2 SV=1)

HSP 1 Score: 186.037 bits (471), Expect = 1.758e-46
Identity = 88/100 (88.00%), Postives = 93/100 (93.00%), Query Frame = 2
Query:  434 GKLNRGLSVVDSYRLLKEGKELTDDEFFLSSALGWCIEWLQAYFLVLDDIMDGSHTRRGQPCWFRVPKVGMIAANDGVLLRNHISRILKNHFRDKPYYVD 733
            GKLNRGLSVVDSY+LLK G EL+DDE FLSSALGWCIEWLQAYFLVLDDIMD SHTRRGQPCWFR+PKVGMIAANDG+LLRNH+ RILK HFR KPYYVD
Sbjct:   48 GKLNRGLSVVDSYQLLKGG-ELSDDEIFLSSALGWCIEWLQAYFLVLDDIMDESHTRRGQPCWFRLPKVGMIAANDGILLRNHVPRILKKHFRGKPYYVD 146          

HSP 2 Score: 79.7221 bits (195), Expect = 1.774e-14
Identity = 33/45 (73.33%), Postives = 41/45 (91.11%), Query Frame = 2
Query:  131 SDLKSRFIEVYGVLKQELLNDPAFEFDHDSRQWVDRMLDYNVPGG 265
            +DLKS+F++VY  LK EL+NDPAFEFD DSRQW+++MLDYNVPGG
Sbjct:    4 TDLKSKFLKVYDTLKSELINDPAFEFDDDSRQWIEKMLDYNVPGG 48          
BLAST of CK932947 vs. ExPASy Swiss-Prot
Match: FPPS2_PARAR (Farnesyl pyrophosphate synthetase 2 OS=Parthenium argentatum GN=FPS2 PE=2 SV=1)

HSP 1 Score: 182.185 bits (461), Expect = 2.538e-45
Identity = 84/100 (84.00%), Postives = 92/100 (92.00%), Query Frame = 2
Query:  434 GKLNRGLSVVDSYRLLKEGKELTDDEFFLSSALGWCIEWLQAYFLVLDDIMDGSHTRRGQPCWFRVPKVGMIAANDGVLLRNHISRILKNHFRDKPYYVD 733
            GKLNRGLSV+DSY+LLK GK LTDDE F +SALGWC+EWLQAYFLVLDDIMD SHTRRGQPCWFR+PKVGMIAANDG++LRNH+ RILK HFR KPYYVD
Sbjct:   47 GKLNRGLSVIDSYQLLKGGK-LTDDEIFHASALGWCVEWLQAYFLVLDDIMDESHTRRGQPCWFRLPKVGMIAANDGIILRNHVPRILKKHFRGKPYYVD 145          

HSP 2 Score: 78.1814 bits (191), Expect = 5.162e-14
Identity = 31/45 (68.89%), Postives = 41/45 (91.11%), Query Frame = 2
Query:  131 SDLKSRFIEVYGVLKQELLNDPAFEFDHDSRQWVDRMLDYNVPGG 265
            +D++S+F++VY  LK EL+NDPAFEFD DSRQW+++MLDYNVPGG
Sbjct:    3 TDIRSKFLQVYDTLKSELINDPAFEFDDDSRQWIEKMLDYNVPGG 47          
BLAST of CK932947 vs. ExPASy Swiss-Prot
Match: FPPS1_ARATH (Farnesyl pyrophosphate synthetase 1, mitochondrial OS=Arabidopsis thaliana GN=FPS1 PE=2 SV=2)

HSP 1 Score: 182.185 bits (461), Expect = 2.538e-45
Identity = 83/100 (83.00%), Postives = 91/100 (91.00%), Query Frame = 2
Query:  434 GKLNRGLSVVDSYRLLKEGKELTDDEFFLSSALGWCIEWLQAYFLVLDDIMDGSHTRRGQPCWFRVPKVGMIAANDGVLLRNHISRILKNHFRDKPYYVD 733
            GKLNRGLSVVDS++LLK+G +LT+ E FLS ALGWCIEWLQAYFLVLDDIMD S TRRGQPCWFRVP+VGM+A NDG+LLRNHI RILK HFRDKPYYVD
Sbjct:   88 GKLNRGLSVVDSFKLLKQGNDLTEQEVFLSCALGWCIEWLQAYFLVLDDIMDNSVTRRGQPCWFRVPQVGMVAINDGILLRNHIHRILKKHFRDKPYYVD 187          

HSP 2 Score: 70.0922 bits (170), Expect = 1.406e-11
Identity = 31/45 (68.89%), Postives = 38/45 (84.44%), Query Frame = 2
Query:  131 SDLKSRFIEVYGVLKQELLNDPAFEFDHDSRQWVDRMLDYNVPGG 265
            +DLKS F+ VY VLK +LL+DP+FEF ++SR WVDRMLDYNV GG
Sbjct:   44 TDLKSTFLNVYSVLKSDLLHDPSFEFTNESRLWVDRMLDYNVRGG 88          
BLAST of CK932947 vs. ExPASy Swiss-Prot
Match: FPPS1_PARAR (Farnesyl pyrophosphate synthetase 1 OS=Parthenium argentatum GN=FPS1 PE=2 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 1.645e-44
Identity = 83/100 (83.00%), Postives = 93/100 (93.00%), Query Frame = 2
Query:  434 GKLNRGLSVVDSYRLLKEGKELTDDEFFLSSALGWCIEWLQAYFLVLDDIMDGSHTRRGQPCWFRVPKVGMIAANDGVLLRNHISRILKNHFRDKPYYVD 733
            GKLNRGLSV+DSY+LLK G ELTD+E FL++ALGWCIEWLQAYFLVLDDIMD SHTRRGQPCWFR+PKVGMIAANDG++LRN++ RILK HFR KPYYVD
Sbjct:   47 GKLNRGLSVIDSYQLLKGG-ELTDNEIFLAAALGWCIEWLQAYFLVLDDIMDESHTRRGQPCWFRLPKVGMIAANDGIILRNNVPRILKKHFRGKPYYVD 145          

HSP 2 Score: 82.8037 bits (203), Expect = 2.096e-15
Identity = 36/45 (80.00%), Postives = 41/45 (91.11%), Query Frame = 2
Query:  131 SDLKSRFIEVYGVLKQELLNDPAFEFDHDSRQWVDRMLDYNVPGG 265
            SDLKSRF++VY  LK EL+NDPAFEFD DSRQWV++MLDYNVPGG
Sbjct:    3 SDLKSRFLQVYDTLKSELINDPAFEFDDDSRQWVEKMLDYNVPGG 47          
BLAST of CK932947 vs. ExPASy Swiss-Prot
Match: FPPS2_ARATH (Farnesyl pyrophosphate synthetase 2 OS=Arabidopsis thaliana GN=FPS2 PE=2 SV=1)

HSP 1 Score: 179.104 bits (453), Expect = 2.149e-44
Identity = 83/100 (83.00%), Postives = 90/100 (90.00%), Query Frame = 2
Query:  434 GKLNRGLSVVDSYRLLKEGKELTDDEFFLSSALGWCIEWLQAYFLVLDDIMDGSHTRRGQPCWFRVPKVGMIAANDGVLLRNHISRILKNHFRDKPYYVD 733
            GKLNRGLSVVDSY+LLK+G++LT+ E FLS ALGWCIEWLQAYFLVLDDIMD S TRRGQPCWFR PKVGMIA NDG+LLRNHI RILK HFR+ PYYVD
Sbjct:   46 GKLNRGLSVVDSYKLLKQGQDLTEKETFLSCALGWCIEWLQAYFLVLDDIMDNSVTRRGQPCWFRKPKVGMIAINDGILLRNHIHRILKKHFREMPYYVD 145          

HSP 2 Score: 75.0998 bits (183), Expect = 4.370e-13
Identity = 32/46 (69.57%), Postives = 40/46 (86.96%), Query Frame = 2
Query:  128 MSDLKSRFIEVYGVLKQELLNDPAFEFDHDSRQWVDRMLDYNVPGG 265
            M+DLKS F++VY VLK +LL DP+FEF H+SRQW++RMLDYNV GG
Sbjct:    1 MADLKSTFLDVYSVLKSDLLQDPSFEFTHESRQWLERMLDYNVRGG 46          
BLAST of CK932947 vs. ExPASy Swiss-Prot
Match: FPPS_MAIZE (Farnesyl pyrophosphate synthetase OS=Zea mays GN=FPS PE=2 SV=1)

HSP 1 Score: 171.014 bits (432), Expect = 5.852e-42
Identity = 77/102 (75.49%), Postives = 88/102 (86.27%), Query Frame = 2
Query:  428 LAGKLNRGLSVVDSYRLLKEGKELTDDEFFLSSALGWCIEWLQAYFLVLDDIMDGSHTRRGQPCWFRVPKVGMIAANDGVLLRNHISRILKNHFRDKPYYVD 733
            L GK NRGLSVVDSY+LLK    L ++E FL+  LGWCIEWLQA+FLVLDDIMD SHTRRGQPCWFRVP+VG+IAANDG++LRNHISRIL+ HF+ KPYY D
Sbjct:   52 LGGKCNRGLSVVDSYKLLKGADALGEEETFLACTLGWCIEWLQAFFLVLDDIMDDSHTRRGQPCWFRVPQVGLIAANDGIILRNHISRILRRHFKGKPYYAD 153          
BLAST of CK932947 vs. ExPASy Swiss-Prot
Match: CPPAS_TANCI (Chrysanthemyl diphosphate synthase, chloroplastic OS=Tanacetum cinerariifolium PE=1 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 1.220e-39
Identity = 75/99 (75.76%), Postives = 88/99 (88.89%), Query Frame = 2
Query:  434 GKLNRGLSVVDSYRLLKEGKELTDDEFFLSSALGWCIEWLQAYFLVLDDIMDGSHTRRGQPCWFRVPKVGMIAANDGVLLRNHISRILKNHFRDKPYYV 730
            GK+ RG SVVDSY+LLK G+ELT+DE FL+ ALGWC EWLQA+ LVLDDIMDGSHTRRGQPCWFR+P+VG++A NDGVLLRNH+ RILK +F+ KPYYV
Sbjct:  100 GKMVRGYSVVDSYQLLK-GEELTEDEAFLACALGWCTEWLQAFILVLDDIMDGSHTRRGQPCWFRLPEVGVVAINDGVLLRNHVHRILKKYFQGKPYYV 197          

HSP 2 Score: 73.559 bits (179), Expect = 1.271e-12
Identity = 31/45 (68.89%), Postives = 40/45 (88.89%), Query Frame = 2
Query:  131 SDLKSRFIEVYGVLKQELLNDPAFEFDHDSRQWVDRMLDYNVPGG 265
            S+L S+F++VY  LK EL++DP+FEFD DSRQWV+RM+DYNVPGG
Sbjct:   56 SNLDSQFMQVYETLKSELIHDPSFEFDDDSRQWVERMIDYNVPGG 100          
The following BLAST results are available for this feature:
BLAST of CK932947 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 23
Match NameE-valueIdentityDescription
FPPS1_LUPAL1.878e-4889.00Farnesyl pyrophosphate synthetase 1 OS=Lupinus alb... [more]
FPPS_HELAN3.542e-4788.00Farnesyl pyrophosphate synthetase OS=Helianthus an... [more]
FPPS2_LUPAL7.890e-4785.00Farnesyl pyrophosphate synthetase 2 OS=Lupinus alb... [more]
FPPS_ARTAN1.758e-4688.00Farnesyl pyrophosphate synthetase OS=Artemisia ann... [more]
FPPS2_PARAR2.538e-4584.00Farnesyl pyrophosphate synthetase 2 OS=Parthenium ... [more]
FPPS1_ARATH2.538e-4583.00Farnesyl pyrophosphate synthetase 1, mitochondrial... [more]
FPPS1_PARAR1.645e-4483.00Farnesyl pyrophosphate synthetase 1 OS=Parthenium ... [more]
FPPS2_ARATH2.149e-4483.00Farnesyl pyrophosphate synthetase 2 OS=Arabidopsis... [more]
FPPS_MAIZE5.852e-4275.49Farnesyl pyrophosphate synthetase OS=Zea mays GN=F... [more]
CPPAS_TANCI1.220e-3975.76Chrysanthemyl diphosphate synthase, chloroplastic ... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004349_F05 Developing fruit juice sac at 38 DAFB Citrus sinensis cDNA clone JSJune0004_IIIF_F05 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK932947 ID=CK932947; Name=CK932947; organism=Citrus sinensis; type=EST; length=735bp
GGGGCTCTCACAAACCAACTCTCTCTCTNNNNNTCTCTTCTCTGTATTTT
GCTTTCTTTCATTCTGTTTCTCTCTTTTTTTTCTTACTTTTTTTTTGGTG
GTGGGTCGCAAAAGCGGAAGGTAAAAAATGAGTGATCTGAAGTCAAGATT
CATTGAGGTGTATGGCGTTTTGAAACAAGAGCTGCTGAATGATCCTGCAT
TTGAGTTCGATCACGATTCTCGCCAATGGGTTGATCGTATGCTGGATTAC
AATGTACCTGGAGGTTAGATAAAAATGTTATATATCTTGTACATTTAAGG
AAGCTTCTGTTTTGTTTGCTTGTGCTGGGCATGTTGGACATCCTCTTCCA
AGAAAGAGGAACTTCCTTTGTTTGAATAGAAAATATGAAAACTTCACTTA
ATTTAACCATCATTACCGATTGTATTCTTGGCAGGAAAGCTGAACCGAGG
GCTATCTGTTGTTGACAGCTACAGGTTACTGAAAGAAGGAAAAGAACTAA
CAGATGATGAATTCTTTCTCTCATCTGCACTAGGCTGGTGCATCGAATGG
CTTCAAGCATATTTTCTTGTTCTTGATGATATCATGGATGGCTCTCATAC
ACGCCGTGGTCAACCTTGCTGGTTCAGAGTCCCCAAGGTTGGTATGATTG
CTGCAAATGATGGTGTCTTACTACGCAACCATATCTCCAGAATTCTCAAG
AATCATTTTAGAGACAAGCCTTATTACGTGGACCT
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