CK933311

Overview
NameCK933311
Unique NameCK933311
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length944
Libraries
Library NameType
Developing fruit juice sac at 38 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CK933311 vs. ExPASy Swiss-Prot
Match: PSA3_SPIOL (Proteasome subunit alpha type-3 OS=Spinacia oleracea GN=PAG1 PE=2 SV=1)

HSP 1 Score: 441.039 bits (1133), Expect = 4.536e-123
Identity = 212/228 (92.98%), Postives = 221/228 (96.93%), Query Frame = 2
Query:   56 MSSIGTGYDLSVTTFSPDGRVFQIEYAAKAVDNSGTVIGIKCKDGIVMGVEKLIASKMMLPGSNRRIHSVHRHSGMAVAGLAADGRQIVTRAKSEATNYESVYGEPIPVKELAQRVASYVHLCTLYWWLRPFGCGVILGGYDRDGPQLYMIEPSGISYRYFGAAIGKGRQAAKTEIEKLKLSEMTCRQGVIEVAKIIYGVHDEAKDKAFELEMSWVCDESNRQHQKVP 739
            MSSIGTGYDLSVTTFSPDGRVFQIEYAAKAVDNSGT +GIKCKDGIV+GVEKLI SKMMLPGSNRRIHSVHRHSGMAVAGLAADGRQIV RAKSEATNYESVYGE +PVKELA RVASYVHLCTLYWWLRPFGCGVILGGYDRDGPQLYM+EPSGISYRYFGAAIGKG+QAAKTEIEKLKLSEMTCR+G+IEVAKIIY VHDEAKDKAFELEMSW+CDES R+HQKVP
Sbjct:    1 MSSIGTGYDLSVTTFSPDGRVFQIEYAAKAVDNSGTAVGIKCKDGIVLGVEKLIQSKMMLPGSNRRIHSVHRHSGMAVAGLAADGRQIVARAKSEATNYESVYGEAVPVKELADRVASYVHLCTLYWWLRPFGCGVILGGYDRDGPQLYMVEPSGISYRYFGAAIGKGKQAAKTEIEKLKLSEMTCREGIIEVAKIIYKVHDEAKDKAFELEMSWICDESKREHQKVP 228          
BLAST of CK933311 vs. ExPASy Swiss-Prot
Match: PSA3_ARATH (Proteasome subunit alpha type-3 OS=Arabidopsis thaliana GN=PAG1 PE=1 SV=2)

HSP 1 Score: 433.721 bits (1114), Expect = 7.242e-121
Identity = 208/228 (91.23%), Postives = 222/228 (97.37%), Query Frame = 2
Query:   56 MSSIGTGYDLSVTTFSPDGRVFQIEYAAKAVDNSGTVIGIKCKDGIVMGVEKLIASKMMLPGSNRRIHSVHRHSGMAVAGLAADGRQIVTRAKSEATNYESVYGEPIPVKELAQRVASYVHLCTLYWWLRPFGCGVILGGYDRDGPQLYMIEPSGISYRYFGAAIGKGRQAAKTEIEKLKLSEMTCRQGVIEVAKIIYGVHDEAKDKAFELEMSWVCDESNRQHQKVP 739
            MSSIGTGYDLSVTTFSPDGRVFQIEYAAKAVDNSGTV+GIKCKDGIVMGVEKLIASKMMLPGSNRRIHSVHRH+GMAVAGLAADGRQIV RAKSEA +YESVYG+ +PVKEL++RVASYVHLCTLYWWLRPFGCGVILGGYDRDGPQLYMIEPSGISYRYFGAAIGKG+QAAKTEIEKL LSEMTC++GVIEVAKIIY +HDEAKDKAFELEMSW+C+ES R+HQKVP
Sbjct:    1 MSSIGTGYDLSVTTFSPDGRVFQIEYAAKAVDNSGTVVGIKCKDGIVMGVEKLIASKMMLPGSNRRIHSVHRHAGMAVAGLAADGRQIVARAKSEARSYESVYGDAVPVKELSERVASYVHLCTLYWWLRPFGCGVILGGYDRDGPQLYMIEPSGISYRYFGAAIGKGKQAAKTEIEKLNLSEMTCKEGVIEVAKIIYKLHDEAKDKAFELEMSWICEESKREHQKVP 228          
BLAST of CK933311 vs. ExPASy Swiss-Prot
Match: PSA3_ORYSJ (Proteasome subunit alpha type-3 OS=Oryza sativa subsp. japonica GN=PAG1 PE=2 SV=1)

HSP 1 Score: 431.795 bits (1109), Expect = 2.752e-120
Identity = 204/228 (89.47%), Postives = 220/228 (96.49%), Query Frame = 2
Query:   56 MSSIGTGYDLSVTTFSPDGRVFQIEYAAKAVDNSGTVIGIKCKDGIVMGVEKLIASKMMLPGSNRRIHSVHRHSGMAVAGLAADGRQIVTRAKSEATNYESVYGEPIPVKELAQRVASYVHLCTLYWWLRPFGCGVILGGYDRDGPQLYMIEPSGISYRYFGAAIGKGRQAAKTEIEKLKLSEMTCRQGVIEVAKIIYGVHDEAKDKAFELEMSWVCDESNRQHQKVP 739
            MSSIGTGYDLSVTTFSPDGRVFQ+EYA KAVDNSGTV+GIKCKDGIV+GVEKL+ SKMML GSNRRIHSVH HSG+AVAGLAADGRQIV+RAKSEA +YE VYGEPI VKELA RVASYVHLCTLYWWLRPFGCGVILGGYDRDGPQLYMIEPSG+SY+YFGAA+GKGRQAAKTEIEKLKLSE+TCR+G++EVAKIIYGVHDEAKDKAFELE+SW+CDESNRQHQKVP
Sbjct:    1 MSSIGTGYDLSVTTFSPDGRVFQVEYATKAVDNSGTVVGIKCKDGIVLGVEKLVTSKMMLEGSNRRIHSVHWHSGLAVAGLAADGRQIVSRAKSEAASYEKVYGEPISVKELADRVASYVHLCTLYWWLRPFGCGVILGGYDRDGPQLYMIEPSGVSYKYFGAALGKGRQAAKTEIEKLKLSELTCREGIVEVAKIIYGVHDEAKDKAFELELSWICDESNRQHQKVP 228          
BLAST of CK933311 vs. ExPASy Swiss-Prot
Match: PSA3_ACACA (Proteasome subunit alpha type-3 (Fragment) OS=Acanthamoeba castellanii PE=2 SV=1)

HSP 1 Score: 313.153 bits (801), Expect = 1.427e-84
Identity = 150/226 (66.37%), Postives = 187/226 (82.74%), Query Frame = 2
Query:   62 SIGTGYDLSVTTFSPDGRVFQIEYAAKAVDNSGTVIGIKCKDGIVMGVEKLIASKMMLPGSNRRIHSVHRHSGMAVAGLAADGRQIVTRAKSEATNYESVYGEPIPVKELAQRVASYVHLCTLYWWLRPFGCGVILGGYDRDGPQLYMIEPSGISYRYFGAAIGKGRQAAKTEIEKLKLSEMTCRQGVIEVAKIIYGVHDEAKDKAFELEMSWVCDESNRQHQKVP 739
            SIGTGYDLS TTFSPDGRVFQ+EYAAKAVDNSGT +G++ KDG+V+ VEKL+ SKM++P +NRRIH+V RH G+A++GL ADGRQ+V+R ++EAT+Y   YG  I  K L +R++++V L +LY  +RPFG  VILG  D++GPQLYMIEPSGIS+ YFG AIGKG +AAKTEIEKLKLSEMT R+ + E AKIIY VHD+AKDKAFELE+SWVC+E+   H+ VP
Sbjct:    1 SIGTGYDLSSTTFSPDGRVFQVEYAAKAVDNSGTALGLRVKDGVVLAVEKLLVSKMLVPNTNRRIHTVDRHCGLAMSGLVADGRQLVSRGRAEATSYREFYGTDISGKVLNERLSNFVQLYSLYGSVRPFGTSVILGCVDKNGPQLYMIEPSGISWGYFGVAIGKGARAAKTEIEKLKLSEMTAREAIKEAAKIIYSVHDDAKDKAFELELSWVCEETGNLHKFVP 226          
BLAST of CK933311 vs. ExPASy Swiss-Prot
Match: PSA3_DICDI (Proteasome subunit alpha type-3 OS=Dictyostelium discoideum GN=psmA3 PE=2 SV=2)

HSP 1 Score: 308.531 bits (789), Expect = 3.514e-83
Identity = 138/228 (60.53%), Postives = 185/228 (81.14%), Query Frame = 2
Query:   56 MSSIGTGYDLSVTTFSPDGRVFQIEYAAKAVDNSGTVIGIKCKDGIVMGVEKLIASKMMLPGSNRRIHSVHRHSGMAVAGLAADGRQIVTRAKSEATNYESVYGEPIPVKELAQRVASYVHLCTLYWWLRPFGCGVILGGYDRDGPQLYMIEPSGISYRYFGAAIGKGRQAAKTEIEKLKLSEMTCRQGVIEVAKIIYGVHDEAKDKAFELEMSWVCDESNRQHQKVP 739
            MSS+G+GYDL V+T+SPDG++FQ++YA KAV+NSGT++ IK KDG+V+GVEKL+ SKM+  GSNRR+H +  H G+A+AG  AD RQ++TRA+SEA NY+S YG+PIP+K L+QR+ASY H+ TLY  +RPFGC + + G D+ GPQL+++EPSG    YFGA++GKG+QAAK E+EK+K SEMTCR+ + EV++IIY VHDE KDK FELE+ W+  E+N  HQ VP
Sbjct:    1 MSSVGSGYDLYVSTYSPDGKLFQVDYANKAVENSGTLVAIKAKDGVVLGVEKLVPSKMLCSGSNRRVHHIDTHVGVAIAGFLADARQLITRARSEAKNYKSTYGQPIPIKVLSQRIASYNHMHTLYGSVRPFGCSIAITGIDQYGPQLFLVEPSGSCVGYFGASLGKGKQAAKNELEKIKFSEMTCREAIKEVSRIIYSVHDEVKDKDFELELGWISTETNNVHQIVP 228          
BLAST of CK933311 vs. ExPASy Swiss-Prot
Match: PSA3_BOVIN (Proteasome subunit alpha type-3 OS=Bos taurus GN=PSMA3 PE=1 SV=3)

HSP 1 Score: 301.212 bits (770), Expect = 5.610e-81
Identity = 151/229 (65.94%), Postives = 179/229 (78.17%), Query Frame = 2
Query:   56 MSSIGTGYDLSVTTFSPDGRVFQIEYAAKAVDNSGTVIGIKCKDGIVMGVEKLIASKMMLPGSNRRIHSVHRHSGMAVAGLAADGRQIVTRAKSEATNYESVYGEPIPVKELAQRVASYVHLCTLYWWLRPFGCGVILGGYD-RDGPQLYMIEPSGISYRYFGAAIGKGRQAAKTEIEKLKLSEMTCRQGVIEVAKIIYGVHDEAKDKAFELEMSWVCDESNRQHQKVP 739
            MSSIGTGYDLS +TFSPDGRVFQ+EYA KAV+NS T IGI+CKDG+V GVEKL+ SK+   GSN+R+ +V RH GMAVAGL AD R +   A+ EA+N+ S +G  IP+K LA RVA YVH  TLY  +RPFGC  +LG Y   DG QLYMI+PSG+SY Y+G AIGK RQAAKTEIEKL++ EMTCR  V EVAKIIY VHDE KDKAFELE+SWV + +N +H+ VP
Sbjct:    1 MSSIGTGYDLSASTFSPDGRVFQVEYAMKAVENSSTAIGIRCKDGVVFGVEKLVLSKLYEEGSNKRLFNVDRHVGMAVAGLLADARSLADIAREEASNFRSNFGYNIPLKHLADRVAMYVHAYTLYSAVRPFGCSFMLGSYSVNDGAQLYMIDPSGVSYGYWGCAIGKARQAAKTEIEKLQMKEMTCRDVVKEVAKIIYIVHDEVKDKAFELELSWVGEITNGRHEIVP 229          
BLAST of CK933311 vs. ExPASy Swiss-Prot
Match: PSA3_HUMAN (Proteasome subunit alpha type-3 OS=Homo sapiens GN=PSMA3 PE=1 SV=2)

HSP 1 Score: 300.827 bits (769), Expect = 7.327e-81
Identity = 151/229 (65.94%), Postives = 179/229 (78.17%), Query Frame = 2
Query:   56 MSSIGTGYDLSVTTFSPDGRVFQIEYAAKAVDNSGTVIGIKCKDGIVMGVEKLIASKMMLPGSNRRIHSVHRHSGMAVAGLAADGRQIVTRAKSEATNYESVYGEPIPVKELAQRVASYVHLCTLYWWLRPFGCGVILGGYD-RDGPQLYMIEPSGISYRYFGAAIGKGRQAAKTEIEKLKLSEMTCRQGVIEVAKIIYGVHDEAKDKAFELEMSWVCDESNRQHQKVP 739
            MSSIGTGYDLS +TFSPDGRVFQ+EYA KAV+NS T IGI+CKDG+V GVEKL+ SK+   GSN+R+ +V RH GMAVAGL AD R +   A+ EA+N+ S +G  IP+K LA RVA YVH  TLY  +RPFGC  +LG Y   DG QLYMI+PSG+SY Y+G AIGK RQAAKTEIEKL++ EMTCR  V EVAKIIY VHDE KDKAFELE+SWV + +N +H+ VP
Sbjct:    1 MSSIGTGYDLSASTFSPDGRVFQVEYAMKAVENSSTAIGIRCKDGVVFGVEKLVLSKLYEEGSNKRLFNVDRHVGMAVAGLLADARSLADIAREEASNFRSNFGYNIPLKHLADRVAMYVHAYTLYSAVRPFGCSFMLGSYSVNDGAQLYMIDPSGVSYGYWGCAIGKARQAAKTEIEKLQMKEMTCRDIVKEVAKIIYIVHDEVKDKAFELELSWVGELTNGRHEIVP 229          
BLAST of CK933311 vs. ExPASy Swiss-Prot
Match: PSA3_RAT (Proteasome subunit alpha type-3 OS=Rattus norvegicus GN=Psma3 PE=1 SV=3)

HSP 1 Score: 298.901 bits (764), Expect = 2.784e-80
Identity = 150/229 (65.50%), Postives = 178/229 (77.73%), Query Frame = 2
Query:   56 MSSIGTGYDLSVTTFSPDGRVFQIEYAAKAVDNSGTVIGIKCKDGIVMGVEKLIASKMMLPGSNRRIHSVHRHSGMAVAGLAADGRQIVTRAKSEATNYESVYGEPIPVKELAQRVASYVHLCTLYWWLRPFGCGVILGGYD-RDGPQLYMIEPSGISYRYFGAAIGKGRQAAKTEIEKLKLSEMTCRQGVIEVAKIIYGVHDEAKDKAFELEMSWVCDESNRQHQKVP 739
            MSSIGTGYDLS +TFSPDGRVFQ+EYA KAV+NS T IGI+CKDG+V GVEKL+ SK+   GSN+R+ +V RH GMAVAGL AD R +   A+ EA+N+ S +G  IP+K LA RVA YVH  TLY  +RPFGC  +LG Y   DG QLYMI+PSG+SY Y+G AIGK RQAAKTEIEKL++ EMTCR  V EVAKIIY VHDE KDKAFELE+SWV + +  +H+ VP
Sbjct:    1 MSSIGTGYDLSASTFSPDGRVFQVEYAMKAVENSSTAIGIRCKDGVVFGVEKLVLSKLYEEGSNKRLFNVDRHVGMAVAGLLADARSLADIAREEASNFRSNFGYNIPLKHLADRVAMYVHAYTLYSAVRPFGCSFMLGSYSVNDGAQLYMIDPSGVSYGYWGCAIGKARQAAKTEIEKLQMKEMTCRDVVKEVAKIIYIVHDEVKDKAFELELSWVGELTKGRHEIVP 229          
BLAST of CK933311 vs. ExPASy Swiss-Prot
Match: PSA3_MOUSE (Proteasome subunit alpha type-3 OS=Mus musculus GN=Psma3 PE=1 SV=3)

HSP 1 Score: 298.901 bits (764), Expect = 2.784e-80
Identity = 150/229 (65.50%), Postives = 178/229 (77.73%), Query Frame = 2
Query:   56 MSSIGTGYDLSVTTFSPDGRVFQIEYAAKAVDNSGTVIGIKCKDGIVMGVEKLIASKMMLPGSNRRIHSVHRHSGMAVAGLAADGRQIVTRAKSEATNYESVYGEPIPVKELAQRVASYVHLCTLYWWLRPFGCGVILGGYD-RDGPQLYMIEPSGISYRYFGAAIGKGRQAAKTEIEKLKLSEMTCRQGVIEVAKIIYGVHDEAKDKAFELEMSWVCDESNRQHQKVP 739
            MSSIGTGYDLS +TFSPDGRVFQ+EYA KAV+NS T IGI+CKDG+V GVEKL+ SK+   GSN+R+ +V RH GMAVAGL AD R +   A+ EA+N+ S +G  IP+K LA RVA YVH  TLY  +RPFGC  +LG Y   DG QLYMI+PSG+SY Y+G AIGK RQAAKTEIEKL++ EMTCR  V EVAKIIY VHDE KDKAFELE+SWV + +  +H+ VP
Sbjct:    1 MSSIGTGYDLSASTFSPDGRVFQVEYAMKAVENSSTAIGIRCKDGVVFGVEKLVLSKLYEEGSNKRLFNVDRHVGMAVAGLLADARSLADIAREEASNFRSNFGYNIPLKHLADRVAMYVHAYTLYSAVRPFGCSFMLGSYSANDGAQLYMIDPSGVSYGYWGCAIGKARQAAKTEIEKLQMKEMTCRDVVKEVAKIIYIVHDEVKDKAFELELSWVGELTKGRHEIVP 229          
BLAST of CK933311 vs. ExPASy Swiss-Prot
Match: PSA3_SCHPO (Probable proteasome subunit alpha type-3 OS=Schizosaccharomyces pombe GN=SPCC1795.04c PE=1 SV=1)

HSP 1 Score: 238.424 bits (607), Expect = 4.466e-62
Identity = 117/228 (51.32%), Postives = 155/228 (67.98%), Query Frame = 2
Query:   56 MSSIGTGYDLSVTTFSPDGRVFQIEYAAKAVDNSGTVIGIKCKDGIVMGVEKLIASKMMLPGSNRRIHSVHRHSGMAVAGLAADGRQIVTRAKSEATNYESVYGEPIPVKELAQRVASYVHLCTLYWWLRPFGCGVILGGYDRDGPQLYMIEPSGISYRYFGAAIGKGRQAAKTEIEKLKLSEMTCRQGVIEVAKIIYGVHDEAKDKAFELEMSWVCDESNRQHQKVP 739
            MSSIGTGYDL +  FSPDGR+FQ EYA KAV+N+ T IGIKC+DG+++ +EK++ SK++ P  N RI SV RH G+A  G   DG+ IV RA+ EAT++   YG PIP   +A R+ +YV L T Y  +RPFG    +  YD +GP LYM+EP+G+ + Y GAA GKGRQ A+ E+EKL  S +  +  V E A+I+Y  HDE  +K  E+EM+WV  E+N  H  VP
Sbjct:    1 MSSIGTGYDLGLF-FSPDGRLFQAEYAYKAVENASTCIGIKCEDGVILALEKVVTSKLLKPRVNNRIGSVDRHIGIATTGFIPDGQHIVKRARDEATSWRDNYGSPIPGTVIADRLGNYVQLFTCYSSVRPFGVMSFVATYDSEGPHLYMVEPNGVYWGYNGAAAGKGRQVARNELEKLNFSSLKMKDAVKEAARILYATHDEENNKEHEIEMTWVGVETNGIHTPVP 227          
The following BLAST results are available for this feature:
BLAST of CK933311 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 176
Match NameE-valueIdentityDescription
PSA3_SPIOL4.536e-12392.98Proteasome subunit alpha type-3 OS=Spinacia olerac... [more]
PSA3_ARATH7.242e-12191.23Proteasome subunit alpha type-3 OS=Arabidopsis tha... [more]
PSA3_ORYSJ2.752e-12089.47Proteasome subunit alpha type-3 OS=Oryza sativa su... [more]
PSA3_ACACA1.427e-8466.37Proteasome subunit alpha type-3 (Fragment) OS=Acan... [more]
PSA3_DICDI3.514e-8360.53Proteasome subunit alpha type-3 OS=Dictyostelium d... [more]
PSA3_BOVIN5.610e-8165.94Proteasome subunit alpha type-3 OS=Bos taurus GN=P... [more]
PSA3_HUMAN7.327e-8165.94Proteasome subunit alpha type-3 OS=Homo sapiens GN... [more]
PSA3_RAT2.784e-8065.50Proteasome subunit alpha type-3 OS=Rattus norvegic... [more]
PSA3_MOUSE2.784e-8065.50Proteasome subunit alpha type-3 OS=Mus musculus GN... [more]
PSA3_SCHPO4.466e-6251.32Probable proteasome subunit alpha type-3 OS=Schizo... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004287_A11 Developing fruit juice sac at 38 DAFB Citrus sinensis cDNA clone JSJune0002_IIF_A11 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK933311 ID=CK933311; Name=CK933311; organism=Citrus sinensis; type=EST; length=944bp
GGGGGAAAGAGGATCGAATTGACTCACAAATCGAAAAAGAAAGAAGAGAG
CAAGAATGAGCAGCATAGGCACAGGCTACGATCTGTCGGTGACCACATTC
TCTCCCGACGGTCGCGTTTTCCAGATCGAATACGCCGCCAAAGCCGTCGA
CAACAGCGGGACTGTGATTGGAATTAAATGCAAAGATGGCATCGTTATGG
GAGTGGAGAAGCTAATAGCGTCGAAGATGATGTTGCCTGGTTCCAATAGA
AGAATCCATTCCGTTCATCGTCACTCCGGCATGGCTGTTGCAGGATTAGC
AGCAGATGGGAGACAAATTGTTACCAGGGCCAAGTCTGAAGCTACAAACT
ATGAAAGTGTATATGGCGAACCAATTCCTGTTAAAGAACTTGCTCAACGA
GTTGCTAGCTATGTGCATCTATGTACTCTATATTGGTGGCTCAGACCTTT
TGGATGTGGTGTAATTCTTGGAGGTTATGACAGAGATGGACCGCAATTGT
ATATGATTGAACCTTCTGGTATCTCCTATAGGTATTTTGGAGCTGCAATT
GGAAAGGGCAGGCAGGCTGCTAAAACAGAGATTGAAAAGTTGAAGCTTTC
TGAAATGACCTGCCGACAAGGGGTCATTGAAGTAGCCAAGATCATTTATG
GAGTGCACGATGAGGCTAAGGACAAGGCCTTTGAACTGGAAATGAGCTGG
GTCTGTGATGAATCAAACCGCCAGCATCAGAAGGTACCAGATGAGCTTCT
TGAGGAAGCTAAGGCAGCCGCCAGAGCAGCGTTGGAAGAGATGGATGCAG
ATTAGTGTAGCAGAGTTGATAATATTTGGTTACTAGTTTGATGCCATTTC
AGGGCTTGTCTTGGAATACTTTTCGAAACATTTTCAAAATATTTANNNAG
ATTGGTGGATTCTCTTAAAAAAAAAAAAANNNAAANNNANAAAA
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