CK934655

Overview
NameCK934655
Unique NameCK934655
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length558
Libraries
Library NameType
Developing fruit peel at 38 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CK934655 vs. ExPASy Swiss-Prot
Match: ASPR_CUCPE (Aspartic proteinase OS=Cucurbita pepo PE=2 SV=1)

HSP 1 Score: 148.288 bits (373), Expect = 2.383e-35
Identity = 67/91 (73.63%), Postives = 78/91 (85.71%), Query Frame = 3
Query:    6 NPMGESIIDCGRIPTMPNVSFTIGDKIFNLSPEQYILKTGEGIAEVCISGFMAFDLPPPRGPLWILGDVFMGVYHTVFDSGKLRIGFAEAA 278
            +PMG+S +DCG++ +MP VSFTIG KIF+L+PE+YILK GEG    CISGF AFD+PPPRGPLWILGDVFMG YHTVFD GKLR+G AEAA
Sbjct:  423 SPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIPPPRGPLWILGDVFMGRYHTVFDFGKLRVGSAEAA 513          
BLAST of CK934655 vs. ExPASy Swiss-Prot
Match: ASPR_HORVU (Phytepsin OS=Hordeum vulgare PE=1 SV=1)

HSP 1 Score: 144.821 bits (364), Expect = 2.634e-34
Identity = 66/91 (72.53%), Postives = 75/91 (82.42%), Query Frame = 3
Query:    6 NPMGESIIDCGRIPTMPNVSFTIGDKIFNLSPEQYILKTGEGIAEVCISGFMAFDLPPPRGPLWILGDVFMGVYHTVFDSGKLRIGFAEAA 278
            +PMGES +DCG + +MP++ FTIG K F L PE+YILK GEG A  CISGF A D+PPPRGPLWILGDVFMG YHTVFD GKLRIGFA+AA
Sbjct:  418 SPMGESAVDCGSLGSMPDIEFTIGGKKFALKPEEYILKVGEGAAAQCISGFTAMDIPPPRGPLWILGDVFMGPYHTVFDYGKLRIGFAKAA 508          
BLAST of CK934655 vs. ExPASy Swiss-Prot
Match: ASPR1_ORYSJ (Aspartic proteinase oryzasin-1 OS=Oryza sativa subsp. japonica GN=Os05g0567100 PE=2 SV=2)

HSP 1 Score: 144.436 bits (363), Expect = 3.441e-34
Identity = 64/91 (70.33%), Postives = 75/91 (82.42%), Query Frame = 3
Query:    6 NPMGESIIDCGRIPTMPNVSFTIGDKIFNLSPEQYILKTGEGIAEVCISGFMAFDLPPPRGPLWILGDVFMGVYHTVFDSGKLRIGFAEAA 278
            +PMGES +DCG + +MP +SFTIG K F L PE+YILK GEG A  CISGF A D+PPPRGPLWILGDVFMG YHTVFD GK+R+GFA++A
Sbjct:  419 SPMGESSVDCGSLASMPEISFTIGGKKFALKPEEYILKVGEGAAAQCISGFTAMDIPPPRGPLWILGDVFMGAYHTVFDYGKMRVGFAKSA 509          
BLAST of CK934655 vs. ExPASy Swiss-Prot
Match: CYPR1_CYNCA (Cyprosin (Fragment) OS=Cynara cardunculus GN=CYPRO1 PE=1 SV=2)

HSP 1 Score: 143.28 bits (360), Expect = 7.665e-34
Identity = 63/91 (69.23%), Postives = 73/91 (80.22%), Query Frame = 3
Query:    6 NPMGESIIDCGRIPTMPNVSFTIGDKIFNLSPEQYILKTGEGIAEVCISGFMAFDLPPPRGPLWILGDVFMGVYHTVFDSGKLRIGFAEAA 278
            +PMGES +DC  + +MPN++FT+G K FNLSPEQY+LK GEG    CISGF A D+ PP GPLWILGDVFMG YHTVFD G LR+GFAEAA
Sbjct:  383 SPMGESAVDCSSLSSMPNIAFTVGGKTFNLSPEQYVLKVGEGATAQCISGFTAMDVAPPHGPLWILGDVFMGQYHTVFDYGNLRVGFAEAA 473          
BLAST of CK934655 vs. ExPASy Swiss-Prot
Match: ASPRX_ORYSJ (Aspartic proteinase OS=Oryza sativa subsp. japonica GN=RAP PE=2 SV=2)

HSP 1 Score: 134.42 bits (337), Expect = 3.561e-31
Identity = 62/91 (68.13%), Postives = 74/91 (81.32%), Query Frame = 3
Query:    6 NPMGESIIDCGRIPTMPNVSFTIGDKIFNLSPEQYILKTGEGIAEVCISGFMAFDLPPPRGPLWILGDVFMGVYHTVFDSGKLRIGFAEAA 278
            +P GES + C +I  MPN++FTI +K F L+PEQYI+K  +G   VCISGFMAFD+PPPRGPLWILGDVFMG YHTVFD GK RIGFA++A
Sbjct:  406 SPNGESTVSCHQISKMPNLAFTIANKTFILTPEQYIVKLEQGGQTVCISGFMAFDIPPPRGPLWILGDVFMGAYHTVFDFGKDRIGFAKSA 496          
BLAST of CK934655 vs. ExPASy Swiss-Prot
Match: CATD_CLUHA (Cathepsin D OS=Clupea harengus GN=ctsd PE=1 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 1.109e-24
Identity = 46/87 (52.87%), Postives = 66/87 (75.86%), Query Frame = 3
Query:   15 GESIIDCGRIPTMPNVSFTIGDKIFNLSPEQYILKTGEGIAEVCISGFMAFDLPPPRGPLWILGDVFMGVYHTVFDSGKLRIGFAEA 275
            GE +IDC ++PT+P +SF +G K ++L+ EQY+LK  +G   +C+SG M  ++PPP GPLWILGDVF+G Y+TVFD    R+GFA++
Sbjct:  309 GEYMIDCKKVPTLPTISFNVGGKTYSLTGEQYVLKESQGGKTICLSGLMGLEIPPPAGPLWILGDVFIGQYYTVFDRESNRVGFAKS 395          
BLAST of CK934655 vs. ExPASy Swiss-Prot
Match: CATD_CHIHA (Cathepsin D OS=Chionodraco hamatus GN=ctsd PE=1 SV=2)

HSP 1 Score: 108.997 bits (271), Expect = 1.602e-23
Identity = 46/87 (52.87%), Postives = 65/87 (74.71%), Query Frame = 3
Query:   15 GESIIDCGRIPTMPNVSFTIGDKIFNLSPEQYILKTGEGIAEVCISGFMAFDLPPPRGPLWILGDVFMGVYHTVFDSGKLRIGFAEA 275
            GE +++C  +P++P +SFT+G +++ L+ EQYILK  +    +C+SGFM  D+P P GPLWILGDVFMG Y+TVFD    R+GFA+A
Sbjct:  309 GEYMVNCDTVPSLPVISFTVGGQVYTLTGEQYILKVTQAGKTMCLSGFMGLDIPAPAGPLWILGDVFMGQYYTVFDRDANRVGFAKA 395          
BLAST of CK934655 vs. ExPASy Swiss-Prot
Match: CATD_HUMAN (Cathepsin D OS=Homo sapiens GN=CTSD PE=1 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 6.087e-23
Identity = 47/88 (53.41%), Postives = 62/88 (70.45%), Query Frame = 3
Query:   15 GESIIDCGRIPTMPNVSFTIGDKIFNLSPEQYILKTGEGIAEVCISGFMAFDLPPPRGPLWILGDVFMGVYHTVFDSGKLRIGFAEAA 278
            GE +I C ++ T+P ++  +G K + LSPE Y LK  +    +C+SGFM  D+PPP GPLWILGDVF+G Y+TVFD    R+GFAEAA
Sbjct:  323 GEYMIPCEKVSTLPAITLKLGGKGYKLSPEDYTLKVSQAGKTLCLSGFMGMDIPPPSGPLWILGDVFIGRYYTVFDRDNNRVGFAEAA 410          
BLAST of CK934655 vs. ExPASy Swiss-Prot
Match: CATD_PIG (Cathepsin D OS=Sus scrofa GN=CTSD PE=1 SV=2)

HSP 1 Score: 106.686 bits (265), Expect = 7.951e-23
Identity = 47/88 (53.41%), Postives = 63/88 (71.59%), Query Frame = 3
Query:   15 GESIIDCGRIPTMPNVSFTIGDKIFNLSPEQYILKTGEGIAEVCISGFMAFDLPPPRGPLWILGDVFMGVYHTVFDSGKLRIGFAEAA 278
            GE +I C ++P++P+V+ T+G K + LS E Y LK  +    +C+SGFM  D+PPP GPLWILGDVF+G Y+TVFD    R+G AEAA
Sbjct:  258 GEYMIPCEKVPSLPDVTVTLGGKKYKLSSENYTLKVSQAGQTICLSGFMGMDIPPPGGPLWILGDVFIGRYYTVFDRDLNRVGLAEAA 345          
BLAST of CK934655 vs. ExPASy Swiss-Prot
Match: CATD_CANFA (Cathepsin D OS=Canis familiaris GN=CTSD PE=2 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 7.951e-23
Identity = 45/87 (51.72%), Postives = 63/87 (72.41%), Query Frame = 3
Query:   15 GESIIDCGRIPTMPNVSFTIGDKIFNLSPEQYILKTGEGIAEVCISGFMAFDLPPPRGPLWILGDVFMGVYHTVFDSGKLRIGFAEA 275
            GE +I C ++ T+P+V+  +G K++ LS E Y LK  +G   +C+SGFM  D+PPP GPLWILGDVF+G Y+TVFD  + R+G A+A
Sbjct:  321 GEYMIPCEKVSTLPDVTLKLGGKLYKLSSEDYTLKVSQGGKTICLSGFMGMDIPPPGGPLWILGDVFIGCYYTVFDRDQNRVGLAQA 407          
The following BLAST results are available for this feature:
BLAST of CK934655 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 64
Match NameE-valueIdentityDescription
ASPR_CUCPE2.383e-3573.63Aspartic proteinase OS=Cucurbita pepo PE=2 SV=1[more]
ASPR_HORVU2.634e-3472.53Phytepsin OS=Hordeum vulgare PE=1 SV=1[more]
ASPR1_ORYSJ3.441e-3470.33Aspartic proteinase oryzasin-1 OS=Oryza sativa sub... [more]
CYPR1_CYNCA7.665e-3469.23Cyprosin (Fragment) OS=Cynara cardunculus GN=CYPRO... [more]
ASPRX_ORYSJ3.561e-3168.13Aspartic proteinase OS=Oryza sativa subsp. japonic... [more]
CATD_CLUHA1.109e-2452.87Cathepsin D OS=Clupea harengus GN=ctsd PE=1 SV=1[more]
CATD_CHIHA1.602e-2352.87Cathepsin D OS=Chionodraco hamatus GN=ctsd PE=1 SV... [more]
CATD_HUMAN6.087e-2353.41Cathepsin D OS=Homo sapiens GN=CTSD PE=1 SV=1[more]
CATD_PIG7.951e-2353.41Cathepsin D OS=Sus scrofa GN=CTSD PE=1 SV=2[more]
CATD_CANFA7.951e-2351.72Cathepsin D OS=Canis familiaris GN=CTSD PE=2 SV=1[more]

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Properties
Property NameValue
Genbank descriptionCGF1004228_D03 Developing fruit peel at 38 DAFB Citrus sinensis cDNA clone P38DAB10002_IIIF_D03 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK934655 ID=CK934655; Name=CK934655; organism=Citrus sinensis; type=EST; length=558bp
GGGCAAATCCGATGGGAGAATCAATTATTGACTGTGGTCGCATTCCAACC
ATGCCAAATGTTTCATTCACCATCGGAGACAAAATTTTCAACCTCTCTCC
AGAGCAGTATATTCTGAAAACCGGAGAAGGGATTGCAGAAGTCTGCATCA
GTGGCTTCATGGCTTTCGACCTGCCTCCTCCAAGGGGTCCTCTGTGGATT
CTGGGAGATGTATTCATGGGGGTTTATCACACTGTTTTCGACTCTGGTAA
GCTCCGGATCGGCTTTGCTGAGGCTGCGTAATGATCTACTTACTGTCAAG
TTTTTATGTAGTAGCAGCGATCATCACCTGCTCATATGAGGAGGAGGGTT
GTAGAATGTAGATTATGGTTTTCATGTACTGTAAAACGATCAGTATGCTT
TTATGCATAACTATCATCATCATCACCACAGATGATATGCTGATATATAC
TTATGTAAAATCCTATCATCCTATGTTTAAAGTACTTCAATTTTATGATA
TTCTGACGTGATAGCTTTACTTATTTGGACAAAAAAAAAAAAAAAAAAAA
AAAAAAAA
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