EY735023

Overview
NameEY735023
Unique NameEY735023
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length866
Libraries
Library NameType
Sweet orange fruit, development stadium (5 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY735023 vs. ExPASy Swiss-Prot
Match: LOXC1_ORYSJ (Lipoxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica GN=CM-LOX1 PE=2 SV=2)

HSP 1 Score: 119.783 bits (299), Expect = 5.735e-27
Identity = 65/135 (48.15%), Postives = 84/135 (62.22%), Query Frame = 3
Query:  360 VSAELDPKTGLDKSTIQDYARKIGADGDGNMQYESEFEVPSGFGEIGAILVENEHHKEMYLKDIVL---DGLPNGPV-NVTCNSWLHSK----HDNKQKRIFFTNKLYLPSPTPDGLKRYRPEEFTFLRRNGTGE 740
            VS+ELD KTG +K+T++ YA  +  D    + YE++F+VPSGFG IGAI+V NE  +EM+L+DI L   DG  N  V  + CNSW+  K         KRIFF NK YLP  TP GL+ YR  +    R +GTGE
Sbjct:  119 VSSELDAKTGKEKATVRSYAHNVDDDDHSVVTYEADFDVPSGFGPIGAIIVTNELRQEMFLEDINLTASDGAGNSTVLPIRCNSWVQPKSVGDEGTPSKRIFFANKTYLPGQTPAGLRSYRKNDLQQKRGDGTGE 253          

HSP 2 Score: 22.7126 bits (47), Expect = 5.735e-27
Identity = 7/15 (46.67%), Postives = 12/15 (80.00%), Query Frame = 1
Query:  736 GKGKTYEKIYDYDVY 780
            G+ +  +++YDYDVY
Sbjct:  252 GEREADDRVYDYDVY 266          
BLAST of EY735023 vs. ExPASy Swiss-Prot
Match: LOXC2_ORYSJ (Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica GN=CM-LOX2 PE=2 SV=1)

HSP 1 Score: 110.923 bits (276), Expect = 1.889e-24
Identity = 62/135 (45.93%), Postives = 84/135 (62.22%), Query Frame = 3
Query:  360 VSAELDPKTGLDKSTIQDYARKIGADGDGNMQYESEFEVPSGFGEIGAILVENEHHKEMYLKDIVL---DGLPNGPV-NVTCNSWLHSKHDNKQ----KRIFFTNKLYLPSPTPDGLKRYRPEEFTFLRRNGTGE 740
            VS+EL+ KTG  K+T+  YA K+  D  G + YE++F+VP+GFG IGA++V NE  +EM+L+D+ L   DG  N  V  + CNSW+  K    +    KRIFF  K YLP  TP GL+ YR E+    R NG G+
Sbjct:  137 VSSELEAKTGKKKATVHSYAHKVDDDDHGVVTYEADFDVPTGFGPIGAVVVTNELGQEMFLEDLNLTAGDGAGNSTVLPIRCNSWVQPKSSIDEGTPGKRIFFA-KAYLPGQTPAGLRSYREEDLKQKRGNGAGQ 270          

HSP 2 Score: 23.0978 bits (48), Expect = 1.889e-24
Identity = 8/19 (42.11%), Postives = 13/19 (68.42%), Query Frame = 1
Query:  724 GMEQGKGKTYEKIYDYDVY 780
            G   G+ +  +++YDYDVY
Sbjct:  265 GNGAGQREADDRVYDYDVY 283          
BLAST of EY735023 vs. ExPASy Swiss-Prot
Match: LOX4_ARATH (Lipoxygenase 4, chloroplastic OS=Arabidopsis thaliana GN=LOX4 PE=2 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 1.900e-24
Identity = 57/131 (43.51%), Postives = 82/131 (62.60%), Query Frame = 3
Query:  360 VSAELDPKTGLDKST----IQDYARKIGADGDGNMQYESEFEVPSGFGEIGAILVENEHHKEMYLKDIVLDGLPNGPVNVTCNSWLHSKHDNKQKRIFFTNKLYLPSPTPDGLKRYRPEEFTFLRRNGTGE 740
            +S ++DPKT   K +    ++D+++K  +  +  + Y +EF V S FG  GAI V N+H KE +L+ I ++G   GPV+  CNSW+ S+ D+  KRI FTN+ YLPS TP GL+  R +E   LR NG GE
Sbjct:  130 MSTQVDPKTNEPKKSKAAVLKDWSKKSNSKAE-RVHYTAEFTVDSAFGSPGAITVTNKHQKEFFLESITIEGFACGPVHFPCNSWVQSQKDHPSKRILFTNQPYLPSETPSGLRTLREKELENLRGNGKGE 259          
BLAST of EY735023 vs. ExPASy Swiss-Prot
Match: LOX3_ARATH (Lipoxygenase 3, chloroplastic OS=Arabidopsis thaliana GN=LOX3 PE=2 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 2.481e-24
Identity = 64/160 (40.00%), Postives = 96/160 (60.00%), Query Frame = 3
Query:  360 VSAELDPKTGLDKST----IQDYARKIGADGDGNMQYESEFEVPSGFGEIGAILVENEHHKEMYLKDIVLDGLPNGPVNVTCNSWLHSKHDNKQKRIFFTNKLYLPSPTPDGLKRYRPEEFTFLRRNGTGEGQNL*KNL*L*CLQWSLKNQKKKTELTRP 827
            +S +LDPKT L K +    ++D+++K     +  + Y +EF V + FG  GAI V N+H KE +L+ I ++G   GPV+  CNSW+ S+ D+  KRIFFTN+ YLP+ TP GL+  R +E   LR +G+G  + L   +    +   L N  K +EL+RP
Sbjct:  124 ISTQLDPKTKLPKKSNAAVLKDWSKKSKTKAE-RVHYTAEFTVDAAFGSPGAITVMNKHQKEFFLESITIEGFALGPVHFPCNSWVQSQKDHPDKRIFFTNQPYLPNETPSGLRVLREKELKNLRGDGSGV-RKLSDRIYDFDVYNDLGNPDKSSELSRP 281          
BLAST of EY735023 vs. ExPASy Swiss-Prot
Match: LOX6_ORYSJ (Probable lipoxygenase 6 OS=Oryza sativa subsp. japonica GN=Os03g0179900 PE=2 SV=2)

HSP 1 Score: 111.309 bits (277), Expect = 7.219e-24
Identity = 59/132 (44.70%), Postives = 86/132 (65.15%), Query Frame = 3
Query:  360 VSAELDPKTGLD----KSTIQDYARKIGADGDGNMQYESEFEVPSGFGEIGAILVENEHHKEMYLKDIVLDG--LPNGPVNVTCNSWLHSKHDNKQKRIFFTNKLYLPSPTPDGLKRYRPEEFTFLRRNGTG 737
            +S ++ P+T       + +I+D+ +K GA GD ++ Y +EF V + FGE GAI V N H++E +L+ IV++G  LP GPV+  CNSW+ S  +   KR+FF+NK YLPS TP GL+  R +E   LR +GTG
Sbjct:  118 ISTKIHPRTKKPMQSGRVSIKDWCQKRGAKGD-HVVYTAEFTVDADFGEPGAIAVANRHNREFFLESIVVEGGGLPCGPVHFACNSWVQSTRELPTKRVFFSNKPYLPSETPPGLRELREKELKDLRGDGTG 248          
BLAST of EY735023 vs. ExPASy Swiss-Prot
Match: LOX23_HORVU (Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare GN=LOX2.3 PE=1 SV=1)

HSP 1 Score: 110.923 bits (276), Expect = 9.428e-24
Identity = 56/129 (43.41%), Postives = 81/129 (62.79%), Query Frame = 3
Query:  360 VSAELDPKTGLDKSTIQDYARKIGADGDGNMQYESEFEVPSGFGEIGAILVENEHHKEMYLKDI--VLDGLPNGPVNVTCNSWLHSKHDNKQKRIFFTNKLYLPSPTPDGLKRYRPEEFTFLRRNGTGE 740
            VS+ELDPKTG ++  ++ +A     +G     YE++  VP+ FG +GA+LVENEHH+EM++KDI  +  G  +  +     SW+HSK D+ + R FFT K YLPS TP G++  R +E   LR +G  E
Sbjct:  106 VSSELDPKTGRERERVKGFAHMTLKEGT----YEAKMSVPASFGPVGAVLVENEHHREMFIKDIKLITGGDESTAITFDVASWVHSKFDDPEPRAFFTVKSYLPSQTPPGIEALRKKELETLRGDGHSE 230          
BLAST of EY735023 vs. ExPASy Swiss-Prot
Match: LOX5_ORYSJ (Putative lipoxygenase 5 OS=Oryza sativa subsp. japonica GN=Os04g0447100 PE=3 SV=2)

HSP 1 Score: 108.997 bits (271), Expect = 3.583e-23
Identity = 55/131 (41.98%), Postives = 78/131 (59.54%), Query Frame = 3
Query:  360 VSAELDPKTGLDK----STIQDYARKIGADGDGNMQYESEFEVPSGFGEIGAILVENEHHKEMYLKDIVLDGLPNGPVNVTCNSWLHSKHDNKQKRIFFTNKLYLPSPTPDGLKRYRPEEFTFLRRNGTGE 740
            VS E DP+ G  K    S +  +  K     +  + Y +EF V +GFGE GA+ V N H +E Y++ IV++G P GP + TCNSW+     ++ +R+FF+N+ YLPS TP GL+  R  E   LR +GTGE
Sbjct:  106 VSTETDPRKGTPKKSKPSALVGWFDKKDVKAE-RVVYTAEFAVDAGFGEPGAVTVLNRHQREFYIESIVVEGFPTGPAHFTCNSWVQPTRVSRDRRVFFSNRPYLPSETPPGLRELRLRELADLRGDGTGE 235          
BLAST of EY735023 vs. ExPASy Swiss-Prot
Match: LOX2_ARATH (Lipoxygenase 2, chloroplastic OS=Arabidopsis thaliana GN=LOX2 PE=1 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 6.757e-22
Identity = 51/121 (42.15%), Postives = 80/121 (66.12%), Query Frame = 3
Query:  360 VSAELDPKTGLDKSTIQDYARKIGADGDGNMQYESEFEVPSGFGEIGAILVENEHHKEMYLKDIVLDGLPNGPVNVTCNSWLHSKHDNKQKRIFFTNKLYLPSPTPDGLKRYRPEEFTFLR 722
            +SA+ D +      T++DYA+++ A+     +YE EFE+P  FG +GAI ++N++H++++LK + L  LP G +  TC SW+  K  +  KRIFF++K YLPS TP+ LK+YR EE   L+
Sbjct:  111 ISAKTDQRI-----TVEDYAQRVWAEAPDE-KYECEFEMPEDFGPVGAIKIQNQYHRQLFLKGVELK-LPGGSITFTCESWVAPKSVDPTKRIFFSDKSYLPSQTPEPLKKYRKEELETLQ 224          
BLAST of EY735023 vs. ExPASy Swiss-Prot
Match: LOX21_HORVU (Lipoxygenase 2.1, chloroplastic OS=Hordeum vulgare GN=LOX2.1 PE=1 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 2.839e-20
Identity = 54/129 (41.86%), Postives = 68/129 (52.71%), Query Frame = 3
Query:  360 VSAELDPKTGLDKSTIQDYARKIGADGDGNMQYESEFEVPSGFGEIGAILVENEHHKEMYLKDIVL--DGLPNGPVNVTCNSWLHSKHDNKQKRIFFTNKLYLPSPTPDGLKRYRPEEFTFLRRNGTGE 740
            VS+ELDP+TG ++  I    +  G   D    YE+ F+VP+ FG IGA+ V N HH EM L DI +   G     V   C SW+   H    KR+FF    YLPS TP G++  R  E   LR  G GE
Sbjct:  120 VSSELDPQTGQEREPISGAVKHSGRVDDEWDMYEATFKVPASFGPIGAVQVTNYHHSEMLLGDIEVFPTGQEESAVTFHCKSWIDPSHCTPDKRVFFPAHSYLPSQTPKGVEGLRKRELEILRGTGCGE 248          
BLAST of EY735023 vs. ExPASy Swiss-Prot
Match: LOX4_SOYBN (Lipoxygenase-4 OS=Glycine max GN=LOX1.5 PE=1 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 1.189e-19
Identity = 45/102 (44.12%), Postives = 61/102 (59.80%), Query Frame = 3
Query:  438 GDGNMQYESEFEVPSGFGEIGAILVENEHHKEMYLKDIVLDGLPN-GPVNVTCNSWLHSKHDNKQKRIFFTNKLYLPSPTPDGLKRYRPEEFTFLRRNGTGE 740
            G G   Y+  FE  S FG  GA  ++N    E YLK ++L+ +PN G ++  CNSW+++  + K  RIFF N  YLPS TP  L +YR EE   +R +GTGE
Sbjct:   89 GAGEQAYDVNFEWDSDFGIPGAFYIKNFMQNEFYLKSLILEDIPNHGTIHFVCNSWVYNSKNYKTDRIFFANNTYLPSETPAPLLKYREEELKNVRGDGTGE 190          

HSP 2 Score: 26.1794 bits (56), Expect = 1.189e-19
Identity = 9/15 (60.00%), Postives = 13/15 (86.67%), Query Frame = 1
Query:  736 GKGKTYEKIYDYDVY 780
            G+ K +++IYDYDVY
Sbjct:  189 GERKEWDRIYDYDVY 203          
The following BLAST results are available for this feature:
BLAST of EY735023 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 27
Match NameE-valueIdentityDescription
LOXC1_ORYSJ5.735e-2748.15Lipoxygenase 7, chloroplastic OS=Oryza sativa subs... [more]
LOXC2_ORYSJ1.889e-2445.93Probable lipoxygenase 8, chloroplastic OS=Oryza sa... [more]
LOX4_ARATH1.900e-2443.51Lipoxygenase 4, chloroplastic OS=Arabidopsis thali... [more]
LOX3_ARATH2.481e-2440.00Lipoxygenase 3, chloroplastic OS=Arabidopsis thali... [more]
LOX6_ORYSJ7.219e-2444.70Probable lipoxygenase 6 OS=Oryza sativa subsp. jap... [more]
LOX23_HORVU9.428e-2443.41Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare... [more]
LOX5_ORYSJ3.583e-2341.98Putative lipoxygenase 5 OS=Oryza sativa subsp. jap... [more]
LOX2_ARATH6.757e-2242.15Lipoxygenase 2, chloroplastic OS=Arabidopsis thali... [more]
LOX21_HORVU2.839e-2041.86Lipoxygenase 2.1, chloroplastic OS=Hordeum vulgare... [more]
LOX4_SOYBN1.189e-1944.12Lipoxygenase-4 OS=Glycine max GN=LOX1.5 PE=1 SV=1[more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-704-075-D09-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY735023 ID=EY735023; Name=EY735023; organism=Citrus sinensis; type=EST; length=866bp
AAATTCTAAAGCATCTTCCTCTTACTTGAAAAACAATGTTGAAGCCACAG
GTTCATCAACCTCAATCTATCAAACCCCTATTTCCATTATCCAAGCCGTT
TCTCCATGGAAATTACGGCCATGCCTTTCGTCCAGTTCAATCAACTTCTA
CACTTTTTAAAGGAAGTCCCAAACTTCGGATTGGTTCTGTTCCCAGAAAC
ACTATCAAAGCTATAGCAACTTCCACTGAAAAATCAATCAAAGTTAAGGC
TGTTGTTACTGTGAAACCAACTGTGGGAGGCTTCCTCTCAAATATTTCTT
TAGACCAAGGGTTAGATGATCTTGGAGATTTGTTTGGTAAATCACTCCTC
TTGGAGCTTGTCAGTGCTGAGCTTGACCCCAAGACGGGATTGGATAAATC
CACCATCCAAGATTATGCGCGCAAAATTGGTGCGGACGGGGATGGAAACA
TGCAATATGAATCAGAATTCGAAGTCCCATCTGGATTTGGAGAAATTGGC
GCAATATTAGTGGAAAATGAGCACCACAAAGAGATGTATTTGAAAGATAT
TGTTCTTGATGGTTTGCCAAATGGTCCTGTCAACGTAACATGTAATTCAT
GGCTTCACTCAAAGCACGACAATAAACAAAAGAGAATTTTCTTTACCAAC
AAGTTGTACTTGCCATCCCCAACACCAGACGGATTGAAGAGATATAGACC
TGAGGAGTTTACATTTTTGCGAAGGAATGGAACAGGGGAAGGGCAAAACC
TATGAAAAAATCTATGATTATGATGTCTACAATGGTCTTTGAAAAACCAA
AAAAAAAAAACTGAATTAACAAGGCCTGGGGTTTGGAGGGAAAAAAAAAA
CCCTTAATCCTAAAAA
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