EY756549

Overview
NameEY756549
Unique NameEY756549
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length609
Libraries
Library NameType
Sweet orange leaf, field plant Bcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY756549 vs. ExPASy Swiss-Prot
Match: FLS_CITUN (Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu GN=FLS PE=1 SV=1)

HSP 1 Score: 301.597 bits (771), Expect = 2.057e-81
Identity = 143/144 (99.31%), Postives = 144/144 (100.00%), Query Frame = 1
Query:   13 AGGDDIEYMLKINYYPPCPRPDLALGVVAHTDLSALTVLVPNEVPGLQVFKDDRWIDAKYIPNALVIHIGDQIEILSNGKYKAVLHRTTVSKDKTRMSWPVFLEPPADTVVGPLPQLVDDENPPKYKAKKFKDYSYCKLNKLPQ 444
            AGGDDIEYMLKINYYPPCPRPDLALGVVAHTDLSALTVLVPNEVPGLQVFKDDRWIDAKYIPNALVIHIGDQIEILSNGKYKAVLHRTTV+KDKTRMSWPVFLEPPADTVVGPLPQLVDDENPPKYKAKKFKDYSYCKLNKLPQ
Sbjct:  192 AGGDDIEYMLKINYYPPCPRPDLALGVVAHTDLSALTVLVPNEVPGLQVFKDDRWIDAKYIPNALVIHIGDQIEILSNGKYKAVLHRTTVNKDKTRMSWPVFLEPPADTVVGPLPQLVDDENPPKYKAKKFKDYSYCKLNKLPQ 335          
BLAST of EY756549 vs. ExPASy Swiss-Prot
Match: FLS_PETHY (Flavonol synthase/flavanone 3-hydroxylase OS=Petunia hybrida GN=FL PE=2 SV=1)

HSP 1 Score: 252.677 bits (644), Expect = 1.096e-66
Identity = 112/144 (77.78%), Postives = 130/144 (90.28%), Query Frame = 1
Query:   13 AGGDDIEYMLKINYYPPCPRPDLALGVVAHTDLSALTVLVPNEVPGLQVFKDDRWIDAKYIPNALVIHIGDQIEILSNGKYKAVLHRTTVSKDKTRMSWPVFLEPPADTVVGPLPQLVDDENPPKYKAKKFKDYSYCKLNKLPQ 444
            AGGD+I Y+LKINYYPPCPRPDLALGVVAHTD+S +T+LVPNEV GLQVFKD  W D KYIPNAL++HIGDQ+EILSNGKYK+V HRTTV+KDKTRMSWPVFLEPP++  VGP+P+L+ + NPPK+K KK+KDY YCKLNKLPQ
Sbjct:  205 AGGDEIVYLLKINYYPPCPRPDLALGVVAHTDMSYITILVPNEVQGLQVFKDGHWYDVKYIPNALIVHIGDQVEILSNGKYKSVYHRTTVNKDKTRMSWPVFLEPPSEHEVGPIPKLLSEANPPKFKTKKYKDYVYCKLNKLPQ 348          
BLAST of EY756549 vs. ExPASy Swiss-Prot
Match: FLS_PETCR (Flavonol synthase/flavanone 3-hydroxylase OS=Petroselinum crispum GN=FLS PE=1 SV=1)

HSP 1 Score: 252.677 bits (644), Expect = 1.096e-66
Identity = 113/144 (78.47%), Postives = 129/144 (89.58%), Query Frame = 1
Query:   13 AGGDDIEYMLKINYYPPCPRPDLALGVVAHTDLSALTVLVPNEVPGLQVFKDDRWIDAKYIPNALVIHIGDQIEILSNGKYKAVLHRTTVSKDKTRMSWPVFLEPPADTVVGPLPQLVDDENPPKYKAKKFKDYSYCKLNKLPQ 444
            +GGDD+ YMLKINYYPPCPRPDLALGVVAHTD+SA+T+LVPNEV GLQV KDD W D KYIPNAL+IHIGDQIEI+SNGKYK+V HRTTV+KDKTRMSWPVFLEPP + + GP+ +L+ DENP K+K KK+KDY YCKLNKLPQ
Sbjct:  194 SGGDDLIYMLKINYYPPCPRPDLALGVVAHTDMSAITILVPNEVQGLQVHKDDHWYDVKYIPNALIIHIGDQIEIMSNGKYKSVYHRTTVNKDKTRMSWPVFLEPPPELLTGPISKLITDENPAKFKTKKYKDYVYCKLNKLPQ 337          
BLAST of EY756549 vs. ExPASy Swiss-Prot
Match: FLS_EUSGR (Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma grandiflorum GN=FLS PE=2 SV=1)

HSP 1 Score: 251.521 bits (641), Expect = 2.441e-66
Identity = 112/144 (77.78%), Postives = 132/144 (91.67%), Query Frame = 1
Query:   13 AGGDDIEYMLKINYYPPCPRPDLALGVVAHTDLSALTVLVPNEVPGLQVFKDDRWIDAKYIPNALVIHIGDQIEILSNGKYKAVLHRTTVSKDKTRMSWPVFLEPPADTVVGPLPQLVDDENPPKYKAKKFKDYSYCKLNKLPQ 444
            AGGDD+ Y++KINYYPPCPRPDLALGV AHTD+SA+TVLVPNEVPGLQV+KD  W D KYIPNAL++HIGDQ+EI+SNGKYK+V HRTTV+K+KTRMSWPVFLEPP D  VGP+P+LV++ENP K+K KK+KDY+YCKLNKLPQ
Sbjct:  192 AGGDDLVYLMKINYYPPCPRPDLALGV-AHTDMSAITVLVPNEVPGLQVYKDGHWYDCKYIPNALIVHIGDQVEIMSNGKYKSVYHRTTVNKEKTRMSWPVFLEPPPDHEVGPIPKLVNEENPAKFKTKKYKDYAYCKLNKLPQ 334          
BLAST of EY756549 vs. ExPASy Swiss-Prot
Match: FLS_MALDO (Flavonol synthase/flavanone 3-hydroxylase OS=Malus domestica GN=FLS PE=2 SV=1)

HSP 1 Score: 247.284 bits (630), Expect = 4.604e-65
Identity = 113/144 (78.47%), Postives = 128/144 (88.89%), Query Frame = 1
Query:   13 AGGDDIEYMLKINYYPPCPRPDLALGVVAHTDLSALTVLVPNEVPGLQVFKDDRWIDAKYIPNALVIHIGDQIEILSNGKYKAVLHRTTVSKDKTRMSWPVFLEPPADTVVGPLPQLVDDENPPKYKAKKFKDYSYCKLNKLPQ 444
            AGGD++EY+LKINYYPPCPRPDLALGVVAHTD+S +T+LVPN+V GLQ  KD RW D KYIPNALVIHIGDQ+EI+SNGKY +VLHRTTV+KDKTR+SWPVFLEPPAD VVGP PQLV+  N PKYK KK+ DY YCK+NKLPQ
Sbjct:  194 AGGDNLEYLLKINYYPPCPRPDLALGVVAHTDMSTVTILVPNDVQGLQACKDGRWYDVKYIPNALVIHIGDQMEIMSNGKYTSVLHRTTVNKDKTRISWPVFLEPPADHVVGPHPQLVNAVNQPKYKTKKYGDYVYCKINKLPQ 337          
BLAST of EY756549 vs. ExPASy Swiss-Prot
Match: FLS1_ARATH (Flavonol synthase/flavanone 3-hydroxylase OS=Arabidopsis thaliana GN=FLS1 PE=1 SV=1)

HSP 1 Score: 238.424 bits (607), Expect = 2.139e-62
Identity = 108/142 (76.06%), Postives = 124/142 (87.32%), Query Frame = 1
Query:   16 GGDDIEYMLKINYYPPCPRPDLALGVVAHTDLSALTVLVPNEVPGLQVFKDDRWIDAKYIPNALVIHIGDQIEILSNGKYKAVLHRTTVSKDKTRMSWPVFLEPPADTVVGPLPQLVDDENPPKYKAKKFKDYSYCKLNKLP 441
            GG+  EYM+KINYYPPCPRPDLALGV AHTDLS +T+LVPNEVPGLQVFKDD W DA+YIP+A+++HIGDQI  LSNG+YK VLHRTTV K+KTRMSWPVFLEPP + +VGPLP+L  D+NPPK+K   FKDYSY KLNKLP
Sbjct:  193 GGEMAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQVFKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKEKTRMSWPVFLEPPREKIVGPLPELTGDDNPPKFKPFAFKDYSYRKLNKLP 334          
BLAST of EY756549 vs. ExPASy Swiss-Prot
Match: FLS_SOLTU (Flavonol synthase/flavanone 3-hydroxylase OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 233.417 bits (594), Expect = 6.880e-61
Identity = 106/144 (73.61%), Postives = 124/144 (86.11%), Query Frame = 1
Query:   13 AGGDDIEYMLKINYYPPCPRPDLALGVVAHTDLSALTVLVPNEVPGLQVFKDDRWIDAKYIPNALVIHIGDQIEILSNGKYKAVLHRTTVSKDKTRMSWPVFLEPPADTVVGPLPQLVDDENPPKYKAKKFKDYSYCKLNKLPQ 444
            AG +DI YMLKINYYPPCPRPDLALGVVAHTD+S +T+LVPNEV   QVFKD  W D  YIPNA+++HIGDQ+EILSNGKYK+V HRTTV+K KTRMSWPVFLEP ++  VGP+P L+++ NPPK+K KK+KDY YCKLNKLPQ
Sbjct:  209 AGSEDIVYMLKINYYPPCPRPDLALGVVAHTDMSYITLLVPNEV---QVFKDGHWYDVNYIPNAIIVHIGDQVEILSNGKYKSVYHRTTVNKYKTRMSWPVFLEPSSEHEVGPIPNLINEANPPKFKTKKYKDYVYCKLNKLPQ 349          
BLAST of EY756549 vs. ExPASy Swiss-Prot
Match: FLS_MATIN (Flavonol synthase/flavanone 3-hydroxylase (Fragment) OS=Matthiola incana PE=2 SV=1)

HSP 1 Score: 175.637 bits (444), Expect = 1.702e-43
Identity = 76/135 (56.30%), Postives = 105/135 (77.78%), Query Frame = 1
Query:   34 YMLKINYYPPCPRPDLALGVVAHTDLSALTVLVPNEVPGLQVFKDDRWIDAKYIPNALVIHIGDQIEILSNGKYKAVLHRTTVSKDKTRMSWPVFLEPPADTVVGPLPQLVDDENPPKYKAKKFKDYSYCKLNKL 438
            Y++ IN+YPP P  D   G+  HTD++ LT+++ NE+PGLQVFKDD WI+ +YIP+A++++IGDQI +LSNGKYK VLH+TTV K+KTRMSWPV + P  D VVGPLP+L  +++PPK+K   +KDY + K+  L
Sbjct:  153 YVMNINHYPPYPHSDSFNGLEPHTDINGLTLIITNEIPGLQVFKDDHWIEVEYIPSAIIVNIGDQIMMLSNGKYKNVLHKTTVDKEKTRMSWPVLVSPTYDMVVGPLPELTSEDDPPKFKPIAYKDYVHNKITFL 287          
BLAST of EY756549 vs. ExPASy Swiss-Prot
Match: LDOX_PETHY (Leucoanthocyanidin dioxygenase OS=Petunia hybrida GN=ANT17 PE=2 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 1.142e-39
Identity = 72/139 (51.80%), Postives = 103/139 (74.10%), Query Frame = 1
Query:   16 GGDDIEYMLKINYYPPCPRPDLALGVVAHTDLSALTVLVPNEVPGLQVFKDDRWIDAKYIPNALVIHIGDQIEILSNGKYKAVLHRTTVSKDKTRMSWPVFLEPPADTVV-GPLPQLVDDENPPKYKAKKFKDYSYCKL 429
            G +D+   +KINYYP CP+P+LALGV AHTD+SALT ++ N VPGLQ+F + +W+ AK +PN++++HIGD IEILSNGKYK++LHR  V+K+K R SW +F EPP + ++  PLP+ V +  PP++  + F  +   KL
Sbjct:  208 GMEDLLLQMKINYYPKCPQPELALGVEAHTDVSALTFILHNMVPGLQLFYEGQWVTAKCVPNSIIMHIGDTIEILSNGKYKSILHRGVVNKEKVRFSWAIFCEPPKEKIILKPLPETVTEAEPPRFPPRTFAQHMAHKL 346          
BLAST of EY756549 vs. ExPASy Swiss-Prot
Match: LDOX_PERFR (Leucoanthocyanidin dioxygenase OS=Perilla frutescens GN=ANS PE=2 SV=1)

HSP 1 Score: 162.54 bits (410), Expect = 1.491e-39
Identity = 72/139 (51.80%), Postives = 104/139 (74.82%), Query Frame = 1
Query:   16 GGDDIEYMLKINYYPPCPRPDLALGVVAHTDLSALTVLVPNEVPGLQVFKDDRWIDAKYIPNALVIHIGDQIEILSNGKYKAVLHRTTVSKDKTRMSWPVFLEPPADTVV-GPLPQLVDDENPPKYKAKKFKDYSYCKL 429
            G +D+   +KIN+YP CP+P+LALG  AHTD+SALT ++ N VPGLQ+F +D+W+ AK +PN++++HIGD +EILSNGKYK++LHR  V+K+K R+SW VF EPP + +V  PLP+ V +  PP++  + F  +   KL
Sbjct:  210 GAEDLIVQMKINFYPKCPQPELALGWEAHTDVSALTFILHNMVPGLQLFYEDKWVTAKCVPNSIIMHIGDTLEILSNGKYKSILHRGLVNKEKVRISWAVFCEPPKEKIVLQPLPETVSEVEPPRFPPRTFAQHLKHKL 348          
The following BLAST results are available for this feature:
BLAST of EY756549 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 85
Match NameE-valueIdentityDescription
FLS_CITUN2.057e-8199.31Flavonol synthase/flavanone 3-hydroxylase OS=Citru... [more]
FLS_PETHY1.096e-6677.78Flavonol synthase/flavanone 3-hydroxylase OS=Petun... [more]
FLS_PETCR1.096e-6678.47Flavonol synthase/flavanone 3-hydroxylase OS=Petro... [more]
FLS_EUSGR2.441e-6677.78Flavonol synthase/flavanone 3-hydroxylase OS=Eusto... [more]
FLS_MALDO4.604e-6578.47Flavonol synthase/flavanone 3-hydroxylase OS=Malus... [more]
FLS1_ARATH2.139e-6276.06Flavonol synthase/flavanone 3-hydroxylase OS=Arabi... [more]
FLS_SOLTU6.880e-6173.61Flavonol synthase/flavanone 3-hydroxylase OS=Solan... [more]
FLS_MATIN1.702e-4356.30Flavonol synthase/flavanone 3-hydroxylase (Fragmen... [more]
LDOX_PETHY1.142e-3951.80Leucoanthocyanidin dioxygenase OS=Petunia hybrida ... [more]
LDOX_PERFR1.491e-3951.80Leucoanthocyanidin dioxygenase OS=Perilla frutesce... [more]

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Properties
Property NameValue
Genbank descriptionCS13-C1-001-009-H05-CT.F Sweet orange leaf, field plant B Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY756549 ID=EY756549; Name=EY756549; organism=Citrus sinensis; type=EST; length=609bp
CCACGCGTCCGAGCGGGAGGAGACGACATTGAGTACATGCTCAAGATTAA
TTATTATCCGCCATGCCCACGTCCTGATCTTGCTCTTGGAGTGGTGGCCC
ACACTGATCTTTCTGCCCTCACCGTTCTTGTTCCCAATGAAGTTCCTGGT
CTGCAAGTCTTTAAGGACGACCGTTGGATCGATGCCAAGTACATCCCCAA
CGCTCTCGTCATCCACATCGGGGACCAGATCGAGATTCTGAGCAATGGCA
AGTACAAAGCTGTGCTGCATAGAACCACTGTTAGCAAGGACAAGACGAGA
ATGTCGTGGCCAGTTTTCTTGGAGCCACCTGCGGATACGGTGGTGGGTCC
CCTTCCTCAGCTGGTTGACGATGAAAATCCTCCAAAGTACAAGGCCAAGA
AGTTCAAGGATTACAGTTACTGTAAACTTAACAAACTTCCTCAGTAGATT
TTTCATTATGAGAAAAATAACACCAGTATCCGCTGGCCGCTCATGTTTTA
TCTCGTCACTGTACGTTTATCGATTCTTGTTTGTTGTTGCGATCTTGTTT
CTGAAATGTAATCGCGGGTATTAGCAATAATAAATAATCCTTTATTTTTA
TTTATTTTG
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