EY720039

Overview
NameEY720039
Unique NameEY720039
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length913
Libraries
Library NameType
Sweet orange fruit, development stadium (4 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY720039 vs. ExPASy Swiss-Prot
Match: FLS_CITUN (Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu GN=FLS PE=1 SV=1)

HSP 1 Score: 425.246 bits (1092), Expect = 2.469e-118
Identity = 221/267 (82.77%), Postives = 227/267 (85.02%), Query Frame = 2
Query:  110 MEVERVQAIASLSHSNGTIPAEFVRPEKEQPASATYHGPAPEIPTIDLDDPVQDRLVRSIAEASREWGIFQVTNHGIPSDLIGKLQAVGKEFFELPQEEKEVYSRPADAKDVQGYGTKLQKEVEGKKSWVDHLFHRVWPPSSINYRFWPNNPPSYRAVNEEYAKYMREVVDKLFTYXXXXXXXXXXXXKEAAGGDDIEYMLKINYYPPCPRPDLALGVVA-TLIFPPSRSCAHESSGLQSFK--RPLDRCQYNPTLSSSHRG-QIEI 898
            MEVERVQAIASLSHSNGTIPAEF+RPEKEQPAS TYHGPAPEIPTIDLDDPVQDRLVRSIAEASREWGIFQVTNHGIPSDLI KLQAVGKEFFELPQEEKEVYSRPADAKDVQGYGTKLQKEVEGKKSWVDHLFHRVWPPSSINYRFWP NPPSYRAVNEEYAKYMREVVDKLFTY            KEAAGGDDIEYMLKINYYPPCPRPDLALGVVA T +   +    +E  GLQ FK  R +D  +Y P     H G QIEI
Sbjct:    1 MEVERVQAIASLSHSNGTIPAEFIRPEKEQPASTTYHGPAPEIPTIDLDDPVQDRLVRSIAEASREWGIFQVTNHGIPSDLICKLQAVGKEFFELPQEEKEVYSRPADAKDVQGYGTKLQKEVEGKKSWVDHLFHRVWPPSSINYRFWPKNPPSYRAVNEEYAKYMREVVDKLFTYLSLGLGVEGGVLKEAAGGDDIEYMLKINYYPPCPRPDLALGVVAHTDLSALTVLVPNEVPGLQVFKDDRWID-AKYIPNALVIHIGDQIEI 266          
BLAST of EY720039 vs. ExPASy Swiss-Prot
Match: FLS_EUSGR (Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma grandiflorum GN=FLS PE=2 SV=1)

HSP 1 Score: 303.138 bits (775), Expect = 1.415e-81
Identity = 148/265 (55.85%), Postives = 188/265 (70.94%), Query Frame = 2
Query:  110 MEVERVQAIASLSHSNGTIPAEFVRPEKEQPASATYHGPAPEIPTIDLDDPVQDRLVRSIAEASREWGIFQVTNHGIPSDLIGKLQAVGKEFFELPQEEKEVYSRPADAKDVQGYGTKLQKEVEGKKSWVDHLFHRVWPPSSINYRFWPNNPPSYRAVNEEYAKYMREVVDKLFTYXXXXXXXXXXXXKEAAGGDDIEYMLKINYYPPCPRPDLALGVVATLIFPPSRSCAHESSGLQSFK-RPLDRCQYNPTLSSSHRG-QIEI 898
            MEV+RVQ IASLS    TIPAE++R E EQP  +T HG   E+P IDL D  + ++V  ++EAS+EWGIFQV NHGIP+++I KLQ VGK FFELPQEEKE+ ++P  ++ ++GYGT+LQKEV+GKK WVDHLFH++WPPS+INY+FWP NPP+YR  NEEYAK ++ VVD LF Y            K+ AGGDD+ Y++KINYYPPCPRPDLALGV  T +   +    +E  GLQ +K      C+Y P     H G Q+EI
Sbjct:    1 MEVQRVQEIASLSKVIDTIPAEYIRSENEQPVISTVHGVVLEVPVIDLSDSDEKKIVGLVSEASKEWGIFQVVNHGIPNEVIRKLQEVGKHFFELPQEEKELIAKPEGSQSIEGYGTRLQKEVDGKKGWVDHLFHKIWPPSAINYQFWPKNPPAYREANEEYAKRLQLVVDNLFKYLSLGLDLEPNSFKDGAGGDDLVYLMKINYYPPCPRPDLALGVAHTDMSAITVLVPNEVPGLQVYKDGHWYDCKYIPNALIVHIGDQVEI 265          
BLAST of EY720039 vs. ExPASy Swiss-Prot
Match: FLS_PETHY (Flavonol synthase/flavanone 3-hydroxylase OS=Petunia hybrida GN=FL PE=2 SV=1)

HSP 1 Score: 301.212 bits (770), Expect = 5.376e-81
Identity = 147/249 (59.04%), Postives = 181/249 (72.69%), Query Frame = 2
Query:  110 MEVERVQAIASLSHSNGTIPAEFVRPEKEQPASATYHGPAPEIPTIDLDDPVQDRLVRSIAEASREWGIFQVTNHGIPSDLIGKLQAVGKEFFE-LPQEEKEVYSRPADAKDVQGYGTKLQKEVEGKKSWVDHLFHRVWPPSSINYRFWPNNPPSYRAVNEEYAKYMREVVDKLFTYXXXXXXXXXXXXKEAAGGDDIEYMLKINYYPPCPRPDLALGVVA-------TLIFPPSRSCAHESSGLQSFK 832
            MEV RVQAIASLS    TIP+E++R E EQPA+ T HG   ++P IDL DP ++++V+ IA+AS+EWGIFQ+ NHGIP + I  LQ VGKEFFE +PQEEKE+ ++   + D++GYGT LQKEVEGKK WVDHLFH++WPPS++NYR+WP NPPSYR  NEEY K MREVVD++F               EAAGGD+I Y+LKINYYPPCPRPDLALGVVA       T++ P      +E  GLQ FK
Sbjct:   13 MEVARVQAIASLSKCMDTIPSEYIRSENEQPAATTLHGVVLQVPVIDLRDPDENKMVKLIADASKEWGIFQLINHGIPDEAIADLQKVGKEFFEHVPQEEKELIAKTPGSNDIEGYGTSLQKEVEGKKGWVDHLFHKIWPPSAVNYRYWPKNPPSYREANEEYGKRMREVVDRIFKSLSLGLGLEGHEMIEAAGGDEIVYLLKINYYPPCPRPDLALGVVAHTDMSYITILVP------NEVQGLQVFK 255          
BLAST of EY720039 vs. ExPASy Swiss-Prot
Match: FLS1_ARATH (Flavonol synthase/flavanone 3-hydroxylase OS=Arabidopsis thaliana GN=FLS1 PE=1 SV=1)

HSP 1 Score: 290.041 bits (741), Expect = 1.239e-77
Identity = 139/248 (56.05%), Postives = 174/248 (70.16%), Query Frame = 2
Query:  110 MEVERVQAIASLSHSNGTIPAEFVRPEKEQPASATYHGPAPEIPTIDLDDPVQDRLVRSIAEASREWGIFQVTNHGIPSDLIGKLQAVGKEFFELPQEEKEVYSRPADAKDVQGYGTKLQKEVEGKKSWVDHLFHRVWPPSSINYRFWPNNPPSYRAVNEEYAKYMREVVDKLFTYXXXXXXXXXXXXKEAAGGDDIEYMLKINYYPPCPRPDLALGVVA-------TLIFPPSRSCAHESSGLQSFK 832
            MEVERVQ I+S S     IP EF+R EKEQPA  T+ GP P IP +DL DP ++ + R++ +AS EWG+FQV NHGIP++LI +LQ VG++FFELP  EKE  ++P D+KD++GYGTKLQK+ EGKK+WVDHLFHR+WPPS +NYRFWP NPP YR VNEEYA +++++ + L               KE  GG+  EYM+KINYYPPCPRPDLALGV A       TL+ P      +E  GLQ FK
Sbjct:    1 MEVERVQDISSSSLLTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEESVRRAVVKASEEWGLFQVVNHGIPTELIRRLQDVGRKFFELPSSEKESVAKPEDSKDIEGYGTKLQKDPEGKKAWVDHLFHRIWPPSCVNYRFWPKNPPEYREVNEEYAVHVKKLSETLLGILSDGLGLKRDALKEGLGGEMAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVP------NEVPGLQVFK 242          
BLAST of EY720039 vs. ExPASy Swiss-Prot
Match: FLS_PETCR (Flavonol synthase/flavanone 3-hydroxylase OS=Petroselinum crispum GN=FLS PE=1 SV=1)

HSP 1 Score: 289.271 bits (739), Expect = 2.114e-77
Identity = 147/250 (58.80%), Postives = 173/250 (69.20%), Query Frame = 2
Query:  110 MEVERVQAIASLSHSNGTIPAEFVRPEKEQPASATYHGPAPEIPTIDL--DDPVQDRLVRSIAEASREWGIFQVTNHGIPSDLIGKLQAVGKEFFELPQEEKEVYSRPADAKDVQGYGTKLQKEVEGKKSWVDHLFHRVWPPSSINYRFWPNNPPSYRAVNEEYAKYMREVVDKLFTYXXXXXXXXXXXXKEAAGGDDIEYMLKINYYPPCPRPDLALGVVA-------TLIFPPSRSCAHESSGLQSFK 832
            MEVERVQAI+ +S    TIP+E++R E EQPA  T  G   ++P IDL   +  ++ LV  IAEASREWGIFQV NHGIP D I KLQ VGKEFFELPQ+EKEV ++P   + V+GYGTKLQKE+ GKK WVDHLFH +WP S++NY FWPNNPP YR  NEEYA  +R VVDKLF              K+A+GGDD+ YMLKINYYPPCPRPDLALGVVA       T++ P      +E  GLQ  K
Sbjct:    1 MEVERVQAISKMSRCMDTIPSEYIRSESEQPAVTTMQGVVLQVPVIDLGSSNNTEENLVELIAEASREWGIFQVVNHGIPDDAIAKLQKVGKEFFELPQQEKEVIAKPEGYQGVEGYGTKLQKELGGKKGWVDHLFHIIWPKSAVNYNFWPNNPPLYREANEEYAVALRGVVDKLFEALSLGIGLEKHELKKASGGDDLIYMLKINYYPPCPRPDLALGVVAHTDMSAITILVP------NEVQGLQVHK 244          
BLAST of EY720039 vs. ExPASy Swiss-Prot
Match: FLS_SOLTU (Flavonol synthase/flavanone 3-hydroxylase OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 283.493 bits (724), Expect = 1.160e-75
Identity = 134/223 (60.09%), Postives = 167/223 (74.89%), Query Frame = 2
Query:  110 MEVERVQAIASLSHSNGTIPAEFVRPEKEQPASATYHGPAPEIPTIDLD--DPVQDRLVRSIAEASREWGIFQVTNHGIPSDLIGKLQAVGKEFFE-LPQEEKEVYSRPADAKDVQGYGTKLQKEVEGKKSWVDHLFHRVWPPSSINYRFWPNNPPSYRAVNEEYAKYMREVVDKLFTYXXXXXXXXXXXXKEAAGGDDIEYMLKINYYPPCPRPDLALGVVA 769
            MEV RVQAI+S++    TIP+E++R E EQPA+ T  G   E+P ID+   D  +++LV+ I EAS+EWGIFQV NHGIP ++I  LQ VGKEFFE +PQEEKE+ ++   A+ ++GYGT LQKE+EGKK WVDHLFH++WPPS+INYR+WP NPPSYR  NEEYAK++R+V D +F               EAAG +DI YMLKINYYPPCPRPDLALGVVA
Sbjct:   15 MEVARVQAISSITKCMDTIPSEYIRSENEQPAATTLQGVVLEVPVIDISNVDDDEEKLVKEIVEASKEWGIFQVINHGIPDEVIENLQKVGKEFFEEVPQEEKELIAKKPGAQSLEGYGTSLQKEIEGKKGWVDHLFHKIWPPSAINYRYWPKNPPSYREANEEYAKWLRKVADGIFRSLSLGLGLEGHEMMEAAGSEDIVYMLKINYYPPCPRPDLALGVVA 237          
BLAST of EY720039 vs. ExPASy Swiss-Prot
Match: FLS_MALDO (Flavonol synthase/flavanone 3-hydroxylase OS=Malus domestica GN=FLS PE=2 SV=1)

HSP 1 Score: 277.33 bits (708), Expect = 8.314e-74
Identity = 130/220 (59.09%), Postives = 163/220 (74.09%), Query Frame = 2
Query:  125 VQAIASLSHSN-GTIPAEFVRPEKEQPASATYHGPAPEIPTIDLDDPVQDRLVRSIAEASREWGIFQVTNHGIPSDLIGKLQAVGKEFFELPQEEKEVYSRPADAKDVQGYGTKLQKEV-EG---KKSWVDHLFHRVWPPSSINYRFWPNNPPSYRAVNEEYAKYMREVVDKLFTYXXXXXXXXXXXXKEAAGGDDIEYMLKINYYPPCPRPDLALGVVA 769
            V+++     SN GTIPAEF+R E EQP   T HG   E+P ID  DP +++L+  I EAS  WG++Q+ NH IPS++I KLQAVGKEFFELPQEEKE Y++P D+  ++GYGTKL KE+ EG   KK WVD+LF+++WPPS +NY+FWP NPPSYR  NEEYAK++  VV+KLF              K+AAGGD++EY+LKINYYPPCPRPDLALGVVA
Sbjct:    3 VESVERERESNEGTIPAEFIRSENEQPGITTVHGKVLEVPIIDFSDPDEEKLIVQITEASSNWGMYQIVNHDIPSEVISKLQAVGKEFFELPQEEKEAYAKPPDSASIEGYGTKLFKEISEGDTTKKGWVDNLFNKIWPPSVVNYQFWPKNPPSYREANEEYAKHLHNVVEKLFRLLSLGLGLEGQELKKAAGGDNLEYLLKINYYPPCPRPDLALGVVA 222          
BLAST of EY720039 vs. ExPASy Swiss-Prot
Match: FLS_MATIN (Flavonol synthase/flavanone 3-hydroxylase (Fragment) OS=Matthiola incana PE=2 SV=1)

HSP 1 Score: 166.007 bits (419), Expect = 2.699e-40
Identity = 81/201 (40.30%), Postives = 126/201 (62.69%), Query Frame = 2
Query:  233 EIPTIDLDDPVQDRLVRSIAEASREWGIFQVTNHGIPSDLIGKLQAVGKEFFELPQEEKEVYSRPADAKDVQGYGTKLQKEVEGKKSWVDHLFHRVWPPSSINYRFWPNNPPSYRAVNEEYAKYMREVVDKLFTYXXXXXXXXXXXXKEAAGGDDIEYMLKINYYPPCPRPDLALGVVA-TLIFPPSRSCAHESSGLQSFK 832
            ++P +DL  P ++ + R++ +AS +WG+FQV NHGIP++LI +LQ VG+EFFELP+ EK   +R  +A  V+GYG +++ +++ +K  VD ++   WPPSS+NYR+WP +PP YR VNEEYA++++ + +K+  +            KE  G     Y++ IN+YPP P  D   G+   T I   +    +E  GLQ FK
Sbjct:    1 QVPVVDLSCPDEELVARTVVKASEDWGVFQVVNHGIPTELIQRLQKVGREFFELPEAEKRSCAR--EAGSVEGYGRRIELDIKKRKGIVDQIYLSTWPPSSVNYRYWPKSPPDYREVNEEYARHVKTLSEKIMEWLSEGLGLGREAIKEVNG---CWYVMNINHYPPYPHSDSFNGLEPHTDINGLTLIITNEIPGLQVFK 196          
BLAST of EY720039 vs. ExPASy Swiss-Prot
Match: LDOX_PERFR (Leucoanthocyanidin dioxygenase OS=Perilla frutescens GN=ANS PE=2 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 1.184e-39
Identity = 81/213 (38.03%), Postives = 118/213 (55.40%), Query Frame = 2
Query:  161 TIPAEFVRPEKEQPA------SATYHGPAPEIPTIDL------DDPVQDRLVRSIAEASREWGIFQVTNHGIPSDLIGKLQAVGKEFFELPQEEKEVYSRPADAKDVQGYGTKLQKEVEGKKSWVDHLFHRVWPPSSINYRFWPNNPPSYRAVNEEYAKYMREVVDKLFT-YXXXXXXXXXXXXKEAAGGDDIEYMLKINYYPPCPRPDLALG 760
            TIP ++VRPE+E  +      +       P++PTIDL      D+  + +    + +A+ +WG+  + NHGIP +LI +++A GKEFFELP EEKE Y+    A +VQGYG+KL     G+  W D+ FH V+P    +   WP  PP Y     EYAK +R +  K+ +              KE  G +D+   +KIN+YP CP+P+LALG
Sbjct:   22 TIPKDYVRPEEELKSIIGNILAEEKSSEGPQLPTIDLEEMDSRDEEGRKKCHEELKKAATDWGVMHLINHGIPEELIDRVKAAGKEFFELPVEEKEAYANDQAAGNVQGYGSKLANNASGQLEWEDYFFHCVYPEHKTDLSIWPTKPPDYIPATSEYAKQLRALATKILSVLSIGLGLEKGRLEKEVGGAEDLIVQMKINFYPKCPQPELALG 234          

HSP 2 Score: 21.9422 bits (45), Expect = 1.184e-39
Identity = 8/10 (80.00%), Postives = 10/10 (100.00%), Query Frame = 1
Query:  769 HTDLSALTFL 798
            HTD+SALTF+
Sbjct:  238 HTDVSALTFI 247          
BLAST of EY720039 vs. ExPASy Swiss-Prot
Match: LDOX_ARATH (Leucoanthocyanidin dioxygenase OS=Arabidopsis thaliana GN=LDOX PE=1 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 6.768e-37
Identity = 80/233 (34.33%), Postives = 125/233 (53.65%), Query Frame = 2
Query:  107 LMEVERVQAIASLSHSNGTIPAEFVRPEKEQPASATYH-----GPAPEIPTIDL------DDPVQDRLVRSIAEASREWGIFQVTNHGIPSDLIGKLQAVGKEFFELPQEEKEVYSRPADAKDVQGYGTKLQKEVEGKKSWVDHLFHRVWPPSSINYRFWPNNPPSYRAVNEEYAKYMREVVDKLF-TYXXXXXXXXXXXXKEAAGGDDIEYMLKINYYPPCPRPDLALGVVA 769
            ++ VERV+++A     +  IP E++RP++E  +             P++PTIDL      D+ +++  +  + +AS +WG+  + NHGIP+DL+ +++  G+EFF L  EEKE Y+       +QGYG+KL     G+  W D+ FH  +P    +   WP  P  Y     EYAK +R +  K+F               KE  G +++   +KINYYP CP+P+LALGV A
Sbjct:    1 MVAVERVESLAKSGIIS--IPKEYIRPKEELESINDVFLEEKKEDGPQVPTIDLKNIESDDEKIRENCIEELKKASLDWGVMHLINHGIPADLMERVKKAGEEFFSLSVEEKEKYANDQATGKIQGYGSKLANNASGQLEWEDYFFHLAYPEEKRDLSIWPKTPSDYIEATSEYAKCLRLLATKVFKALSVGLGLEPDRLEKEVGGLEELLLQMKINYYPKCPQPELALGVEA 231          

HSP 2 Score: 21.9422 bits (45), Expect = 6.768e-37
Identity = 8/10 (80.00%), Postives = 10/10 (100.00%), Query Frame = 1
Query:  769 HTDLSALTFL 798
            HTD+SALTF+
Sbjct:  232 HTDVSALTFI 241          
The following BLAST results are available for this feature:
BLAST of EY720039 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 44
Match NameE-valueIdentityDescription
FLS_CITUN2.469e-11882.77Flavonol synthase/flavanone 3-hydroxylase OS=Citru... [more]
FLS_EUSGR1.415e-8155.85Flavonol synthase/flavanone 3-hydroxylase OS=Eusto... [more]
FLS_PETHY5.376e-8159.04Flavonol synthase/flavanone 3-hydroxylase OS=Petun... [more]
FLS1_ARATH1.239e-7756.05Flavonol synthase/flavanone 3-hydroxylase OS=Arabi... [more]
FLS_PETCR2.114e-7758.80Flavonol synthase/flavanone 3-hydroxylase OS=Petro... [more]
FLS_SOLTU1.160e-7560.09Flavonol synthase/flavanone 3-hydroxylase OS=Solan... [more]
FLS_MALDO8.314e-7459.09Flavonol synthase/flavanone 3-hydroxylase OS=Malus... [more]
FLS_MATIN2.699e-4040.30Flavonol synthase/flavanone 3-hydroxylase (Fragmen... [more]
LDOX_PERFR1.184e-3938.03Leucoanthocyanidin dioxygenase OS=Perilla frutesce... [more]
LDOX_ARATH6.768e-3734.33Leucoanthocyanidin dioxygenase OS=Arabidopsis thal... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-703-001-B12-CT.F Sweet orange fruit, development stadium (4 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY720039 ID=EY720039; Name=EY720039; organism=Citrus sinensis; type=EST; length=913bp
CCACGCGTCCGCGTTACTGCGTCACAGTCGCAGGAAAGGCGCAAGCCAAG
AAAGAGAGAGGGAGAGAAGGAAAAGAAAAGTGGTGGGGTTTTGCTGGAAA
ATAAAATTAATGGAGGTGGAGAGGGTCCAAGCCATTGCTTCCTTATCCCA
TTCAAATGGCACAATTCCAGCAGAGTTCGTAAGACCCGAAAAAGAACAGC
CAGCAAGCGCAACGTACCACGGCCCCGCTCCTGAAATCCCCACGATCGAT
CTCGACGACCCCGTTCAAGACAGACTCGTACGTTCCATCGCGGAAGCCAG
CCGGGAGTGGGGGATTTTCCAGGTTACAAACCACGGGATACCTAGTGACC
TCATCGGTAAACTGCAAGCCGTCGGCAAAGAATTTTTTGAGCTCCCTCAG
GAAGAGAAAGAAGTGTATTCTCGTCCGGCTGATGCAAAAGACGTGCAAGG
ATACGGCACAAAGTTACAGAAAGAAGTCGAAGGAAAGAAATCTTGGGTTG
ATCATCTCTTCCACAGGGTTTGGCCTCCGTCTTCTATCAACTACCGCTTT
TGGCCCAACAACCCTCCTTCTTACCGAGCGGTGAATGAGGAGTATGCAAA
GTACATGAGGGAGGTGGTGGATAAGTTGTTTACGTATCTTTCGTTGGGAC
TGGGTGTCGAAGGAGGTGTTTTGAAAGAAGCAGCGGGAGGAGACGACATT
GAGTACATGCTCAAGATTAATTACTATCCGCCATGCCCACGTCCTGATCT
TGCTCTTGGAGTGGTGGCCACACTGATCTTTCCGCCCTCACGTTCTTGTG
CCCATGAAAGTTCTGGTCTGCAGTCTTTTAAGAGACCGTTGGATCGATGC
CAGTACAATCCGACGCTCTCATCATCACATCGGGGACAGATCGAGATCTG
AGCATGGCAGTAC
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