EY688646

Overview
NameEY688646
Unique NameEY688646
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length486
Libraries
Library NameType
Sweet orange bark, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY688646 vs. ExPASy Swiss-Prot
Match: FAD3E_TOBAC (Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Nicotiana tabacum GN=FAD3 PE=2 SV=1)

HSP 1 Score: 208.379 bits (529), Expect = 1.382e-53
Identity = 90/108 (83.33%), Postives = 102/108 (94.44%), Query Frame = 3
Query:    3 KLPWYRGKEWNYLRGGLTTVDRDYGIFNNIHHDIGTHVIHHLFPQIPHYHLVEATKAAKPVLGKYYREPNKSGPIPVHLIKNLVKSIGEDHFVSDTGEIVYYETDPQL 326
            KLPWYRGKEW+YLRGGLTTVDRDYG+FNNIHHDIGTHVIHHLFPQIPHYHL EATKAAKPVLGKYYREP KSGPIP HL+K+L +S+ +DH+VSD+GEIV+Y+TDP +
Sbjct:  264 KLPWYRGKEWSYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIPHYHLREATKAAKPVLGKYYREPKKSGPIPFHLVKDLTRSMKQDHYVSDSGEIVFYQTDPHI 371          
BLAST of EY688646 vs. ExPASy Swiss-Prot
Match: FAD3C_BRANA (Omega-3 fatty acid desaturase, chloroplastic (Fragment) OS=Brassica napus GN=FAD7 PE=2 SV=1)

HSP 1 Score: 205.682 bits (522), Expect = 8.960e-53
Identity = 90/108 (83.33%), Postives = 100/108 (92.59%), Query Frame = 3
Query:    3 KLPWYRGKEWNYLRGGLTTVDRDYGIFNNIHHDIGTHVIHHLFPQIPHYHLVEATKAAKPVLGKYYREPNKSGPIPVHLIKNLVKSIGEDHFVSDTGEIVYYETDPQL 326
            KLPWYRGKEW+YLRGGLTT+DRDYG+ NNIHHDIGTHVIHHLFPQIPHYHLVEAT+AAKPVLGKYYREP+KSGP+P+HL+  L KSI EDHFVSD G++VYYE DP L
Sbjct:  288 KLPWYRGKEWSYLRGGLTTLDRDYGLINNIHHDIGTHVIHHLFPQIPHYHLVEATEAAKPVLGKYYREPDKSGPLPLHLLGILAKSIKEDHFVSDEGDVVYYEADPNL 395          
BLAST of EY688646 vs. ExPASy Swiss-Prot
Match: FAD3E_PHAAU (Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Phaseolus aureus GN=ARG1 PE=2 SV=1)

HSP 1 Score: 205.297 bits (521), Expect = 1.170e-52
Identity = 91/115 (79.13%), Postives = 104/115 (90.43%), Query Frame = 3
Query:    3 KLPWYRGKEWNYLRGGLTTVDRDYGIFNNIHHDIGTHVIHHLFPQIPHYHLVEATKAAKPVLGKYYREPNKSGPIPVHLIKNLVKSIGEDHFVSDTGEIVYYETDPQLKKFLATK 347
            KLPWYRG+EW+YLRGGLTTVDRDYG  NN+HHDIGTHVIHHLFPQIPHYHLVEATK+AK VLGKYYREP KSGP+P HL+K L++SI +DHFVSDTG+IVYY+TDP+L +   TK
Sbjct:  264 KLPWYRGQEWSYLRGGLTTVDRDYGWINNVHHDIGTHVIHHLFPQIPHYHLVEATKSAKSVLGKYYREPQKSGPLPFHLLKYLLQSISQDHFVSDTGDIVYYQTDPKLHQDSWTK 378          
BLAST of EY688646 vs. ExPASy Swiss-Prot
Match: FAD3C_ARATH (Omega-3 fatty acid desaturase, chloroplastic OS=Arabidopsis thaliana GN=FAD7 PE=1 SV=1)

HSP 1 Score: 205.297 bits (521), Expect = 1.170e-52
Identity = 89/108 (82.41%), Postives = 101/108 (93.52%), Query Frame = 3
Query:    3 KLPWYRGKEWNYLRGGLTTVDRDYGIFNNIHHDIGTHVIHHLFPQIPHYHLVEATKAAKPVLGKYYREPNKSGPIPVHLIKNLVKSIGEDHFVSDTGEIVYYETDPQL 326
            KLPWYRGKEW+YLRGGLTT+DRDYG+ NNIHHDIGTHVIHHLFPQIPHYHLVEAT+AAKPVLGKYYREP+KSGP+P+HL++ L KSI EDH+VSD GE+VYY+ DP L
Sbjct:  330 KLPWYRGKEWSYLRGGLTTLDRDYGLINNIHHDIGTHVIHHLFPQIPHYHLVEATEAAKPVLGKYYREPDKSGPLPLHLLEILAKSIKEDHYVSDEGEVVYYKADPNL 437          
BLAST of EY688646 vs. ExPASy Swiss-Prot
Match: FAD3E_ARATH (Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Arabidopsis thaliana GN=FAD3 PE=2 SV=1)

HSP 1 Score: 204.912 bits (520), Expect = 1.528e-52
Identity = 90/115 (78.26%), Postives = 104/115 (90.43%), Query Frame = 3
Query:    3 KLPWYRGKEWNYLRGGLTTVDRDYGIFNNIHHDIGTHVIHHLFPQIPHYHLVEATKAAKPVLGKYYREPNKSGPIPVHLIKNLVKSIGEDHFVSDTGEIVYYETDPQLKKFLATK 347
            KLPWYRGKEW+YLRGGLTT+DRDYGIFNNIHHDIGTHVIHHLFPQIPHYHLV+ATKAAK VLG+YYREP  SG IP+HL+++LV SI +DH+VSDTG+IV+YETDP L  + + K
Sbjct:  268 KLPWYRGKEWSYLRGGLTTIDRDYGIFNNIHHDIGTHVIHHLFPQIPHYHLVDATKAAKHVLGRYYREPKTSGAIPIHLVESLVASIKKDHYVSDTGDIVFYETDPDLYVYASDK 382          
BLAST of EY688646 vs. ExPASy Swiss-Prot
Match: FAD3D_ARATH (Temperature-sensitive omega-3 fatty acid desaturase, chloroplastic OS=Arabidopsis thaliana GN=FAD8 PE=2 SV=1)

HSP 1 Score: 204.527 bits (519), Expect = 1.996e-52
Identity = 88/108 (81.48%), Postives = 101/108 (93.52%), Query Frame = 3
Query:    3 KLPWYRGKEWNYLRGGLTTVDRDYGIFNNIHHDIGTHVIHHLFPQIPHYHLVEATKAAKPVLGKYYREPNKSGPIPVHLIKNLVKSIGEDHFVSDTGEIVYYETDPQL 326
            KLPWYRGKEW+YLRGGLTT+DRDYG  NNIHHDIGTHVIHHLFPQIPHYHLVEAT+AAKPVLGKYYREP  SGP+P+HL+ +L+KS+ +DHFVSDTG++VYYE DP+L
Sbjct:  323 KLPWYRGKEWSYLRGGLTTLDRDYGWINNIHHDIGTHVIHHLFPQIPHYHLVEATEAAKPVLGKYYREPKNSGPLPLHLLGSLIKSMKQDHFVSDTGDVVYYEADPKL 430          
BLAST of EY688646 vs. ExPASy Swiss-Prot
Match: FAD3E_BRANA (Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Brassica napus GN=FAD3 PE=2 SV=1)

HSP 1 Score: 203.756 bits (517), Expect = 3.405e-52
Identity = 89/115 (77.39%), Postives = 104/115 (90.43%), Query Frame = 3
Query:    3 KLPWYRGKEWNYLRGGLTTVDRDYGIFNNIHHDIGTHVIHHLFPQIPHYHLVEATKAAKPVLGKYYREPNKSGPIPVHLIKNLVKSIGEDHFVSDTGEIVYYETDPQLKKFLATK 347
            KLPWYRGKEW+YLRGGLTT+DRDYGIFNNIHHDIGTHVIHHLFPQIPHYHLV+AT+AAK VLG+YYREP  SG IP+HL+++LV SI +DH+VSDTG+IV+YETDP L  + + K
Sbjct:  265 KLPWYRGKEWSYLRGGLTTIDRDYGIFNNIHHDIGTHVIHHLFPQIPHYHLVDATRAAKHVLGRYYREPKTSGAIPIHLVESLVASIKKDHYVSDTGDIVFYETDPDLYVYASDK 379          
BLAST of EY688646 vs. ExPASy Swiss-Prot
Match: FAD3C_RICCO (Omega-3 fatty acid desaturase, chloroplastic OS=Ricinus communis GN=FAD7A-1 PE=2 SV=1)

HSP 1 Score: 201.445 bits (511), Expect = 1.690e-51
Identity = 88/117 (75.21%), Postives = 103/117 (88.03%), Query Frame = 3
Query:    3 KLPWYRGKEWNYLRGGLTTVDRDYGIFNNIHHDIGTHVIHHLFPQIPHYHLVEATKAAKPVLGKYYREPNKSGPIPVHLIKNLVKSIGEDHFVSDTGEIVYYETDPQLKKFLATKFE 353
            KLPWYRGK W+YLRGGLTT+DRDYG  NNIHHDIGTHVIHHLFPQIPHYHLVEAT+AAKPV+GKYYREP KSGP+P+HL+ +LV+S+ EDH+VSDTG++VYY+ DP+L      K E
Sbjct:  344 KLPWYRGKAWSYLRGGLTTLDRDYGWINNIHHDIGTHVIHHLFPQIPHYHLVEATEAAKPVMGKYYREPKKSGPLPLHLLGSLVRSMKEDHYVSDTGDVVYYQKDPKLSGIGGEKTE 460          
BLAST of EY688646 vs. ExPASy Swiss-Prot
Match: FAD3C_SESIN (Omega-3 fatty acid desaturase, chloroplastic OS=Sesamum indicum GN=FAD7 PE=2 SV=1)

HSP 1 Score: 199.519 bits (506), Expect = 6.422e-51
Identity = 85/108 (78.70%), Postives = 98/108 (90.74%), Query Frame = 3
Query:    3 KLPWYRGKEWNYLRGGLTTVDRDYGIFNNIHHDIGTHVIHHLFPQIPHYHLVEATKAAKPVLGKYYREPNKSGPIPVHLIKNLVKSIGEDHFVSDTGEIVYYETDPQL 326
            KLPWYRGKEW+YLRGGLTT+DRDYG  NNIHHDIGTHVIHHLFPQIPHYHL+EAT+AAKPVLGKYYREP KS P+P HL+ +L +S+  DH+VSD G++VYY+TDPQL
Sbjct:  334 KLPWYRGKEWSYLRGGLTTLDRDYGWINNIHHDIGTHVIHHLFPQIPHYHLIEATEAAKPVLGKYYREPKKSAPLPFHLLGDLTRSLKRDHYVSDVGDVVYYQTDPQL 441          
BLAST of EY688646 vs. ExPASy Swiss-Prot
Match: FAD3E_SOYBN (Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Glycine max GN=FAD3 PE=2 SV=1)

HSP 1 Score: 195.282 bits (495), Expect = 1.211e-49
Identity = 86/108 (79.63%), Postives = 97/108 (89.81%), Query Frame = 3
Query:    3 KLPWYRGKEWNYLRGGLTTVDRDYGIFNNIHHDIGTHVIHHLFPQIPHYHLVEATKAAKPVLGKYYREPNKSGPIPVHLIKNLVKSIGEDHFVSDTGEIVYYETDPQL 326
            KLPWYRGKEW+YLRGGLTTVDRDYG   NIHHDIGTHVIHHLFPQIPHYHLVEAT+AAKPVLG YYREP +S P+P HLIK L++S+ +DHFVSDTG++VYY+TD  L
Sbjct:  267 KLPWYRGKEWSYLRGGLTTVDRDYGWIYNIHHDIGTHVIHHLFPQIPHYHLVEATQAAKPVLGDYYREPERSAPLPFHLIKYLIQSMRQDHFVSDTGDVVYYQTDSLL 374          
The following BLAST results are available for this feature:
BLAST of EY688646 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 18
Match NameE-valueIdentityDescription
FAD3E_TOBAC1.382e-5383.33Omega-3 fatty acid desaturase, endoplasmic reticul... [more]
FAD3C_BRANA8.960e-5383.33Omega-3 fatty acid desaturase, chloroplastic (Frag... [more]
FAD3E_PHAAU1.170e-5279.13Omega-3 fatty acid desaturase, endoplasmic reticul... [more]
FAD3C_ARATH1.170e-5282.41Omega-3 fatty acid desaturase, chloroplastic OS=Ar... [more]
FAD3E_ARATH1.528e-5278.26Omega-3 fatty acid desaturase, endoplasmic reticul... [more]
FAD3D_ARATH1.996e-5281.48Temperature-sensitive omega-3 fatty acid desaturas... [more]
FAD3E_BRANA3.405e-5277.39Omega-3 fatty acid desaturase, endoplasmic reticul... [more]
FAD3C_RICCO1.690e-5175.21Omega-3 fatty acid desaturase, chloroplastic OS=Ri... [more]
FAD3C_SESIN6.422e-5178.70Omega-3 fatty acid desaturase, chloroplastic OS=Se... [more]
FAD3E_SOYBN1.211e-4979.63Omega-3 fatty acid desaturase, endoplasmic reticul... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C2-003-090-G04-CT.F Sweet orange bark, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY688646 ID=EY688646; Name=EY688646; organism=Citrus sinensis; type=EST; length=486bp
ACAAACTACCTTGGTACCGTGGCAAGGAATGGAATTATTTGAGAGGAGGG
CTAACAACAGTGGATAGAGATTATGGAATCTTCAACAACATTCACCATGA
TATCGGCACTCATGTCATTCATCATCTTTTCCCTCAAATCCCACATTATC
ACTTAGTAGAGGCCACAAAGGCAGCAAAGCCAGTGCTGGGAAAGTACTAC
CGTGAGCCGAATAAATCAGGGCCAATTCCAGTCCACTTGATCAAGAATTT
GGTGAAAAGCATTGGCGAAGACCACTTTGTGAGTGACACTGGAGAAATTG
TGTACTATGAGACGGACCCTCAGCTGAAGAAATTTTTAGCAACCAAGTTT
GAAAGATACTTTTAGAGCTTACGTTATTAGTCTTCCTAAGCCAGGGCACA
TAGCCTATATAATGTACTTCCAATAACTCCCTTTGCTTTATGAAGTTTAA
TTCTGCCACTTAAAACTTAAAAAAAAAAAAAAAAAG
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