EY685447

Overview
NameEY685447
Unique NameEY685447
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length991
Libraries
Library NameType
Sweet orange bark, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY685447 vs. ExPASy Swiss-Prot
Match: BYST_NEMVE (Bystin OS=Nematostella vectensis GN=bysl PE=3 SV=1)

HSP 1 Score: 130.568 bits (327), Expect = 1.411e-29
Identity = 65/130 (50.00%), Postives = 92/130 (70.77%), Query Frame = 3
Query:  195 RLLEAFLSKDAGPQVTLADLIIKKIKENDANIAS--GETRPLPKLDESFINLYKGVGEFLSKYTAGKMPGAFEHIPSTQMWEQVLYLTEPEKWTPNAMFPATGIFSSNLNAKKAKRF*KLGLLPRIRDDI 578
            +  E F+S++A  + TLAD+I++KI++    I S   E    P++DE  + ++KGVGE L KY +GK+P AF+ IPS   WE+VL++TEP++W+  A+F AT IF SNLNAK A+RF  L LLPRI+DDI
Sbjct:  125 KAFEMFMSQEAPTRRTLADVIMEKIQDKKTEIESHMSEQSTAPQMDERLVKVFKGVGEILKKYRSGKLPKAFKFIPSLTNWEEVLFITEPDEWSAAALFQATKIFVSNLNAKMAQRFFNLVLLPRIQDDI 254          
BLAST of EY685447 vs. ExPASy Swiss-Prot
Match: BYST_HUMAN (Bystin OS=Homo sapiens GN=BYSL PE=1 SV=3)

HSP 1 Score: 127.102 bits (318), Expect = 1.560e-28
Identity = 70/177 (39.55%), Postives = 106/177 (59.89%), Query Frame = 3
Query:  195 RLLEAFLSKDAGPQVTLADLIIKKIKENDANIASGETR----PLPKLDESFINLYKGVGEFLSKYTAGKMPGAFEHIPSTQMWEQVLYLTEPEKWTPNAMFPATGIFSSNLNAKKAKRF*KLGLLPRIRDDIRKEQE---HGFCSISSFQELSGGNLLHLKRKYCARLRQSGTCQLR 704
            R +E F++K+   + TLAD+I++K+ E    + +  +     P+P+LD   + +Y+GV E LSKY +GK+P AF+ IP+   WEQ+LY+TEPE WT  AM+ AT IF+SNL  + A+RF  L LLPR+RDD+ + +    H + ++       G     +    C    +SGTC LR
Sbjct:  131 RAIEMFMNKNPPARRTLADIIMEKLTEKQTEVETVMSEVSGFPMPQLDPRVLEVYRGVREVLSKYRSGKLPKAFKIIPALSNWEQILYVTEPEAWTAAAMYQATRIFASNLKERMAQRFYNLVLLPRVRDDVAEYKRLNFHLYMALKKALFKPGAWFKGILIPLC----ESGTCTLR 303          
BLAST of EY685447 vs. ExPASy Swiss-Prot
Match: BYST_BOVIN (Bystin OS=Bos taurus GN=BYSL PE=2 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 3.476e-28
Identity = 70/177 (39.55%), Postives = 106/177 (59.89%), Query Frame = 3
Query:  195 RLLEAFLSKDAGPQVTLADLIIKKIKENDANIASGETR----PLPKLDESFINLYKGVGEFLSKYTAGKMPGAFEHIPSTQMWEQVLYLTEPEKWTPNAMFPATGIFSSNLNAKKAKRF*KLGLLPRIRDDIRKEQE---HGFCSISSFQELSGGNLLHLKRKYCARLRQSGTCQLR 704
            R +E F++++   + TLAD+I++K+ E    + +  +     P+P+LD   + +Y+GV E LSKY +GK+P AF+ IP+   WEQ+LY+TEPE WT  AM+ AT IF+SNL  + A+RF  L LLPR+RDDI + +    H + ++       G     +    C    +SGTC LR
Sbjct:  129 RAIEMFMNQNPPARRTLADIIMEKLTEKQTEVETVMSEVSGFPVPQLDPRVLEVYRGVREVLSKYRSGKLPKAFKIIPALSNWEQILYITEPEAWTAAAMYQATRIFASNLKERMAQRFYNLVLLPRVRDDIAEYKRLNFHLYMALKKALFKPGAWFKGILIPLC----ESGTCTLR 301          
BLAST of EY685447 vs. ExPASy Swiss-Prot
Match: BYST_MOUSE (Bystin OS=Mus musculus GN=Bysl PE=1 SV=3)

HSP 1 Score: 125.176 bits (313), Expect = 5.929e-28
Identity = 70/177 (39.55%), Postives = 105/177 (59.32%), Query Frame = 3
Query:  195 RLLEAFLSKDAGPQVTLADLIIKKIKENDANIASGETR----PLPKLDESFINLYKGVGEFLSKYTAGKMPGAFEHIPSTQMWEQVLYLTEPEKWTPNAMFPATGIFSSNLNAKKAKRF*KLGLLPRIRDDIRKEQE---HGFCSISSFQELSGGNLLHLKRKYCARLRQSGTCQLR 704
            R +E F++K+   + TLAD+I++K+ E    + +  +     P+P+LD   + +Y+GV E L KY +GK+P AF+ IP+   WEQ+LY+TEPE WT  AM+ AT IF+SNL  + A+RF  L LLPR+RDDI + +    H + ++       G     +    C    +SGTC LR
Sbjct:  130 RAIEMFMNKNPPVRRTLADIIMEKLTEKQTEVETVMSEVSGFPMPQLDPRVLEVYRGVREVLCKYRSGKLPKAFKVIPALSNWEQILYVTEPEAWTAAAMYQATRIFASNLKERMAQRFYNLVLLPRVRDDIAEYKRLNFHLYMALKKALFKPGAWFKGILIPLC----ESGTCTLR 302          
BLAST of EY685447 vs. ExPASy Swiss-Prot
Match: BYST_MONBE (Bystin OS=Monosiga brevicollis GN=bysl PE=3 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 3.769e-27
Identity = 59/135 (43.70%), Postives = 91/135 (67.41%), Query Frame = 3
Query:  201 LEAFLSKDAGPQVTLADLIIKKIKENDANIASGETRPLPK-LDESFINLYKGVGEFLSKYTAGKMPGAFEHIPSTQMWEQVLYLTEPEKWTPNAMFPATGIFSSNLNAKKAKRF*KLGLLPRIRDDIRKEQEHGF 602
            L AF+  +   + TLAD+I++KI+     +AS  ++  P+ L+   I +Y+GVG+ LS+Y +GK+P AF+ IP  + WE+++Y+TEPE WT  +M+ AT +F+SNL  K A+RF  L LLPR+RDDI + +   F
Sbjct:  112 LRAFMPAEPAQRRTLADIIMEKIQGKRTEVASQVSQTGPRELNPKVIEVYQGVGQVLSRYRSGKLPKAFKIIPRLKNWEEIVYITEPENWTAASMYAATRLFASNLKEKMAQRFYNLILLPRVRDDIAEYKRLNF 246          

HSP 2 Score: 25.0238 bits (53), Expect = 3.769e-27
Identity = 14/41 (34.15%), Postives = 22/41 (53.66%), Query Frame = 1
Query:  589 KNMDFALYRAFKSSLVATCCI*KGNIVPGCGSQGHASLGEA 711
            K ++F LY+A K ++       KG ++P C   G  +L EA
Sbjct:  242 KRLNFHLYQAIKKAIFKPGAFFKGFLLPLC-EAGDCTLREA 281          
BLAST of EY685447 vs. ExPASy Swiss-Prot
Match: BYST_RAT (Bystin OS=Rattus norvegicus GN=Bysl PE=2 SV=3)

HSP 1 Score: 122.094 bits (305), Expect = 5.019e-27
Identity = 69/177 (38.98%), Postives = 104/177 (58.76%), Query Frame = 3
Query:  195 RLLEAFLSKDAGPQVTLADLIIKKIKENDANIASGETR----PLPKLDESFINLYKGVGEFLSKYTAGKMPGAFEHIPSTQMWEQVLYLTEPEKWTPNAMFPATGIFSSNLNAKKAKRF*KLGLLPRIRDDIRKEQE---HGFCSISSFQELSGGNLLHLKRKYCARLRQSGTCQLR 704
            R +E F++K+   + TLAD+I++K+ E    + +  +     P+P+LD   + +Y+GV E L KY +G +P AF+ IP+   WEQ+LY+TEPE WT  AM+ AT IF+SNL  + A+RF  L LLPR+RDDI + +    H + ++       G     +    C    +SGTC LR
Sbjct:  130 RAIEMFMNKNPPVRRTLADIIMEKLTEKQTEVETVMSEVSGFPMPQLDPRVLEVYRGVREVLCKYRSGILPKAFKIIPALSNWEQILYVTEPEAWTAAAMYQATRIFASNLKERMAQRFYNLVLLPRVRDDIAEYKRLNFHLYMALKKALFKPGAWFKGILIPLC----ESGTCTLR 302          
BLAST of EY685447 vs. ExPASy Swiss-Prot
Match: BYN1_CAEEL (Cell adhesion protein byn-1 OS=Caenorhabditis elegans GN=byn-1 PE=2 SV=2)

HSP 1 Score: 117.857 bits (294), Expect = 9.466e-26
Identity = 73/188 (38.83%), Postives = 103/188 (54.79%), Query Frame = 3
Query:  201 LEAFLSKDAGPQVTLADLIIKKI--KENDANIASGETRP----LPKLDESFINLYKGVGEFLSKYTAGKMPGAFEHIPSTQMWEQVLYLTEPEKWTPNAMFPATGIFSSNLNAKKAKRF*KLGLLPRIRDDIRKEQEHGFCSISSFQELSGGNLLHLKRKYCAR--------------LRQSGTCQLR 704
            L  FL KDA    TL D+I  KI  K+NDA +A  +  P    +  +D   + +Y+ +G+++SKY +GK+P AF+ IP    WEQ+L+LT+PE WT  AM+ AT +F+SN+N K  +RF  L LLPR+RDD           I  F++L+     HL +  C                L +SGTC LR
Sbjct:  133 LARFLKKDAIQMSTLYDIIQAKIEAKQNDAELALSQVDPNEFNMRDMDPEVVEMYEQIGQYMSKYRSGKVPKAFKIIPKMINWEQILFLTKPETWTAAAMYQATRLFASNMNPKMCQRFYTLVLLPRLRDD-----------IDEFKKLN----YHLYQALCKAIYKPAAFFKGLILPLLESGTCTLR 305          
BLAST of EY685447 vs. ExPASy Swiss-Prot
Match: BYST_DICDI (Bystin OS=Dictyostelium discoideum GN=bysl PE=3 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 3.805e-23
Identity = 63/180 (35.00%), Postives = 99/180 (55.00%), Query Frame = 3
Query:   69 AEEEQSKRRVXXXXXXXXX-FGGFNETQSQFG-NYXXXXXXXXXRLLEAFLSKDAGP--------QVTLADLIIKKIKENDANIASGETRPLPKLDESFINLYKGVGEFLSKYTAGKMPGAFEHIPSTQMWEQVLYLTEPEKWTPNAMFPATGIFSSNLNAKKAKRF*KLGLLPRIRDDI 578
            A+++ +KRR           F  F++T+SQFG            R+L  F+    G         + TL D+I  K+KE+++   S E    PK+    I++Y  VG+ L  YT+GK+P AF  +P+   WE +LYLT P+KWTP+++  AT +F  + N+K  +RF  + +LPR+RD+I
Sbjct:  117 ADQDDNKRRSNGNDFDENDGFEQFSDTESQFGVGGEVEIDEEDERVLSMFMGGGGGDGQEQQFQTRFTLGDIIESKLKEHESRQVSSENAINPKV----IDVYTKVGKLLETYTSGKIPRAFRILPNFTNWEDLLYLTRPDKWTPHSIRVATKLFCMSTNSKITQRFLSIVVLPRVRDNI 292          

HSP 2 Score: 26.1794 bits (56), Expect = 3.805e-23
Identity = 14/29 (48.28%), Postives = 20/29 (68.97%), Query Frame = 2
Query:  605 LYIELSRALWWQPAAFKKEILCPVAAVRD 691
            LY+ L ++L+ +PAAF K IL P+A   D
Sbjct:  302 LYMALKKSLY-RPAAFYKAILLPLAESGD 329          
BLAST of EY685447 vs. ExPASy Swiss-Prot
Match: YBB9_SCHPO (Uncharacterized protein C13G1.09 OS=Schizosaccharomyces pombe GN=SPBC13G1.09 PE=1 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 1.847e-21
Identity = 55/125 (44.00%), Postives = 77/125 (61.60%), Query Frame = 3
Query:  240 TLADLIIKKIKENDANI-------ASGETRPLPKLDESFINLYKGVGEFLSKYTAGKMPGAFEHIPSTQMWEQVLYLTEPEKWTPNAMFPATGIFSSNLNAKKAKRF*KLGLLPRIRDDIRKEQE 593
            +L+DLI++KI E +A         A  E   LP L    I +Y  VG  LSKY +GK+P AF+ IP+   WE +LYLT P+ WTP+A + AT IF SNL   +A+ F  + +L R+RDDIR+ ++
Sbjct:  156 SLSDLIMQKINEAEARARGEYIPSAEEEENALPPLPPKVIEVYSKVGVLLSKYRSGKIPKAFKIIPTLSNWEDILYLTRPDMWTPHACYEATRIFISNLKPVQAQHFLTVIILERVRDDIRENKK 280          
BLAST of EY685447 vs. ExPASy Swiss-Prot
Match: BYS_DROME (Bystin OS=Drosophila melanogaster GN=bys PE=1 SV=2)

HSP 1 Score: 93.9745 bits (232), Expect = 1.464e-18
Identity = 47/116 (40.52%), Postives = 75/116 (64.66%), Query Frame = 3
Query:  243 LADLIIKKIKENDANI----ASGETRPLPKLDESFINLYKGVGEFLSKYTAGKMPGAFEHIPSTQMWEQVLYLTEPEKWTPNAMFPATGIFSSNLNAKKAKRF*KLGLLPRIRDDI 578
            L+ +I++KI+E +A+I    +   +  + ++D     +Y+GV + L +Y +GK+P AF+ IP  + WEQ+L++TEP  W+  AMF  T IF S L+   A+RF  L LLPR+RDD+
Sbjct:  128 LSKMIMQKIQEKEADIHTKISDEGSLKIEEIDPKVKEMYEGVRDVLKRYRSGKIPKAFKIIPKLRNWEQILFITEPHNWSAAAMFQGTRIFCSVLSQAMAQRFYNLVLLPRVRDDL 243          
The following BLAST results are available for this feature:
BLAST of EY685447 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 11
Match NameE-valueIdentityDescription
BYST_NEMVE1.411e-2950.00Bystin OS=Nematostella vectensis GN=bysl PE=3 SV=1[more]
BYST_HUMAN1.560e-2839.55Bystin OS=Homo sapiens GN=BYSL PE=1 SV=3[more]
BYST_BOVIN3.476e-2839.55Bystin OS=Bos taurus GN=BYSL PE=2 SV=1[more]
BYST_MOUSE5.929e-2839.55Bystin OS=Mus musculus GN=Bysl PE=1 SV=3[more]
BYST_MONBE3.769e-2743.70Bystin OS=Monosiga brevicollis GN=bysl PE=3 SV=1[more]
BYST_RAT5.019e-2738.98Bystin OS=Rattus norvegicus GN=Bysl PE=2 SV=3[more]
BYN1_CAEEL9.466e-2638.83Cell adhesion protein byn-1 OS=Caenorhabditis eleg... [more]
BYST_DICDI3.805e-2335.00Bystin OS=Dictyostelium discoideum GN=bysl PE=3 SV... [more]
YBB9_SCHPO1.847e-2144.00Uncharacterized protein C13G1.09 OS=Schizosaccharo... [more]
BYS_DROME1.464e-1840.52Bystin OS=Drosophila melanogaster GN=bys PE=1 SV=2[more]

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Properties
Property NameValue
Genbank descriptionCS00-C2-003-019-D03-CT.F Sweet orange bark, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY685447 ID=EY685447; Name=EY685447; organism=Citrus sinensis; type=EST; length=991bp
TGATTCAGCAGAAGGAGGTTCTAGAAGAGTCCGAAGAGCCTAACGCTACC
AAAAGCGCCTTCGTTTTTGCCGAAGAAGAGCAGAGCAAGCGACGAGTAGA
AGAAGATGAAGATGATATTGATGATTTCGGTGGTTTTAATGAGACTCAAA
GTCAATTTGGAAATTATGAGGAGGAGATTGATGAGGATGATGAGAGATTA
TTGGAGGCATTTCTATCAAAGGATGCTGGTCCGCAAGTTACACTTGCAGA
CCTCATCATCAAAAAAATAAAAGAAAACGATGCAAACATTGCTTCAGGAG
AAACACGGCCTTTGCCCAAGTTGGATGAGTCATTCATAAATTTGTATAAA
GGAGTTGGCGAGTTTCTCAGTAAATACACAGCAGGAAAAATGCCCGGAGC
GTTTGAGCACATCCCTTCAACACAAATGTGGGAGCAGGTCTTGTACCTTA
CTGAACCCGAGAAATGGACACCTAATGCAATGTTTCCAGCGACAGGAATT
TTCTCTTCCAATCTGAATGCCAAGAAGGCAAAGCGCTTTTAAAAGCTTGG
TTTGCTCCCTCGAATCAGAGATGATATTCGAAAGGAACAAGAACATGGAT
TTTGCTCTATATCGAGCTTTCAAGAGCTCTCTGGTGGCAACCTGCTGCAT
TTAAAAAGGAAATATTGTGCCCGGTTGCGGCAGTCAGGGACATGCCAGCT
TAGGGGAGGCTGGGGGTTCTGTGGGAGCGTCTTTGAAGAGCACCCGCGTC
TCTGGGAGCGGTTGACCTGGGGGGCGTGCTCGGGGTGGGGAGACGGGGAA
AGTGGGGGGAGAAGACGCTTGGCTGCTGTAGGACAAGCCGCAAGACCACC
AGATGTGGGCATGCGCATAAACGAGATGGTGACGGCATTGCGGGACGGGC
TGTCTTACAAAGGAGTACGTGCGTGGAGCGGCTGTCCCGCGGCGAGGGAG
GTGAAGCGGGGCGACGCCGGCGCACTCTGCGACGTGGAAGA
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