CX078059

Overview
NameCX078059
Unique NameCX078059
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length799
Libraries
Library NameType
Parent Washington Navel Orange Callus cDNA Library UCRCS08-2cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX078059 vs. ExPASy Swiss-Prot
Match: 7OMT9_MEDSA (Isoflavone-7-O-methyltransferase 9 OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 209.92 bits (533), Expect = 1.315e-53
Identity = 105/244 (43.03%), Postives = 152/244 (62.30%), Query Frame = 1
Query:   67 IQNEGAKEMFEAQAHLFKIIYNYVSSTSLKCAVELDIPEVIHKHGRPITLPQLVSALEINPTKADGLFKLMRLLVHTGFFSTANVXXXXXXXXXXYALTLTSKLFLKDKPYCLSPVVLTLTDPVFVNPCHFLSRWFRDNELSAYETANDGTVFWDYMAKNPDFNSIYNQAMASDSQLANLIVKDCQPIFQGLGSLVDVGGGTGSFARIISEAFPGIKCTVLDLPHVVPKVPDTDNLKFIAGDMF 798
            I      E+F+AQA L+K IY ++ S SLK AV ++IP +IH HG+PI+L  LVS L++  +K   + +LMR L H GFF               YALT+ S+L ++    CL+P+V  + DP      H L +W  + +L+ +     G+ FWD++ KNP++N+ +N AMASDS+L NL ++DC  +F GL S+VDVGGGTG+ A+II E FP +KC V D P VV  +  ++NL ++ GDMF
Sbjct:    5 INGRKPSEIFKAQALLYKHIYAFIDSMSLKWAVGMNIPNIIHNHGKPISLSNLVSILQVPSSKIGNVRRLMRYLAHNGFFEIIT------KEEESYALTVASELLVRGSDLCLAPMVECVLDPTLSGSYHELKKWIYEEDLTLFGVTL-GSGFWDFLDKNPEYNTSFNDAMASDSKLINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNLTYVGGDMF 241          
BLAST of CX078059 vs. ExPASy Swiss-Prot
Match: 7OMT8_MEDSA (Isoflavone-7-O-methyltransferase 8 OS=Medicago sativa PE=1 SV=1)

HSP 1 Score: 209.534 bits (532), Expect = 1.717e-53
Identity = 105/244 (43.03%), Postives = 152/244 (62.30%), Query Frame = 1
Query:   67 IQNEGAKEMFEAQAHLFKIIYNYVSSTSLKCAVELDIPEVIHKHGRPITLPQLVSALEINPTKADGLFKLMRLLVHTGFFSTANVXXXXXXXXXXYALTLTSKLFLKDKPYCLSPVVLTLTDPVFVNPCHFLSRWFRDNELSAYETANDGTVFWDYMAKNPDFNSIYNQAMASDSQLANLIVKDCQPIFQGLGSLVDVGGGTGSFARIISEAFPGIKCTVLDLPHVVPKVPDTDNLKFIAGDMF 798
            I      E+F+AQA L+K IY ++ S SLK AVE++IP +I  HG+PI+L  LVS L++  +K   + +LMR L H GFF               YALT+ S+L ++    CL+P+V  + DP      H L +W  + +L+ +     G+ FWD++ KNP++N+ +N AMASDS+L NL ++DC  +F GL S+VDVGGGTG+ A+II E FP +KC V D P VV  +  ++NL ++ GDMF
Sbjct:    5 INGRKPSEIFKAQALLYKHIYAFIDSMSLKWAVEMNIPNIIQNHGKPISLSNLVSILQVPSSKIGNVRRLMRYLAHNGFFEIIT------KEEESYALTVASELLVRGSDLCLAPMVECVLDPTLSGSYHELKKWIYEEDLTLFGVTL-GSGFWDFLDKNPEYNTSFNDAMASDSKLINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNLTYVGGDMF 241          
BLAST of CX078059 vs. ExPASy Swiss-Prot
Match: 7OMT6_MEDSA (Isoflavone-7-O-methyltransferase 6 OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 209.534 bits (532), Expect = 1.717e-53
Identity = 105/244 (43.03%), Postives = 152/244 (62.30%), Query Frame = 1
Query:   67 IQNEGAKEMFEAQAHLFKIIYNYVSSTSLKCAVELDIPEVIHKHGRPITLPQLVSALEINPTKADGLFKLMRLLVHTGFFSTANVXXXXXXXXXXYALTLTSKLFLKDKPYCLSPVVLTLTDPVFVNPCHFLSRWFRDNELSAYETANDGTVFWDYMAKNPDFNSIYNQAMASDSQLANLIVKDCQPIFQGLGSLVDVGGGTGSFARIISEAFPGIKCTVLDLPHVVPKVPDTDNLKFIAGDMF 798
            I      E+F+AQA L+K IY ++ S SLK AVE++IP +I  HG+PI+L  LVS L++  +K   + +LMR L H GFF               YALT+ S+L ++    CL+P+V  + DP      H L +W  + +L+ +     G+ FWD++ KNP++N+ +N AMASDS+L NL ++DC  +F GL S+VDVGGGTG+ A+II E FP +KC V D P VV  +  ++NL ++ GDMF
Sbjct:    5 INGRKPSEIFKAQALLYKHIYAFIDSMSLKWAVEMNIPNIIQNHGKPISLSNLVSILQVPSSKIGNVRRLMRYLAHNGFFEIIT------KEEESYALTVASELLVRGSDLCLAPMVECVLDPTLSGSYHELKKWIYEEDLTLFGVTL-GSGFWDFLDKNPEYNTSFNDAMASDSKLINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNLTYVGGDMF 241          
BLAST of CX078059 vs. ExPASy Swiss-Prot
Match: CVMT1_OCIBA (Chavicol O-methyltransferase OS=Ocimum basilicum GN=CVOMT1 PE=1 SV=1)

HSP 1 Score: 207.223 bits (526), Expect = 8.522e-53
Identity = 103/240 (42.92%), Postives = 154/240 (64.17%), Query Frame = 1
Query:   85 KEMFEAQAHLFKIIYNYVSSTSLKCAVELDIPEVIHKHGRPITLPQLVSALEINPTKADGLFKLMRLLVHTGFFSTANVXXXXXXXXXXYALTLTSKLFLKDKPYCLSPVVLTLTDPVFVNPCHFLSRWFR-DNELSAYETANDGTVFWDYMAKNPDFNSIYNQAMASDSQL-ANLIVKDCQPIFQGLGSLVDVGGGTGSFARIISEAFPGIKCTVLDLPHVVPKVPDTDNLKFIAGDMF 798
            +++ +AQAH++  +Y + +S SLKCA++L IP+++HKH  P+TL QL+ A+ IN  K+    +LMR LV++ FF   N           Y LT  S+L LK  P  ++P+V  + DP F NP H++S WF+ +N  + +E AN G  FW+ +A  P     +++AM+ DS+L A+++ KD + +  G+ +LVDVGGG G+ A+ I EA P +KCTVLDLPHVV  +  TD L +I GDMF
Sbjct:   13 EQLLQAQAHVWNHMYAFANSMSLKCAIQLGIPDILHKHDHPMTLSQLLKAIPINKEKSQSFQRLMRALVNSNFFIEEN----SNNQEVCYWLTPASRLLLKGAPLTVAPLVQVVLDPTFTNPWHYMSEWFKHENHATQFEAAN-GCTFWEKLANKPSMGRFFDEAMSCDSRLVAHVLTKDYKHVIDGIRTLVDVGGGNGTMAKAIVEAVPTMKCTVLDLPHVVAGLESTDKLSYIGGDMF 247          
BLAST of CX078059 vs. ExPASy Swiss-Prot
Match: EOMT1_OCIBA (Eugenol O-methyltransferase OS=Ocimum basilicum GN=EOMT1 PE=1 SV=1)

HSP 1 Score: 204.142 bits (518), Expect = 7.215e-52
Identity = 101/239 (42.26%), Postives = 150/239 (62.76%), Query Frame = 1
Query:   85 KEMFEAQAHLFKIIYNYVSSTSLKCAVELDIPEVIHKHGRPITLPQLVSALEINPTKADGLFKLMRLLVHTGFFSTANVXXXXXXXXXXYALTLTSKLFLKDKPYCLSPVVLTLTDPVFVNPCHFLSRWFRDNELSAYETANDGTVFWDYMAKNPDFNSIYNQAMASDSQL-ANLIVKDCQPIFQGLGSLVDVGGGTGSFARIISEAFPGIKCTVLDLPHVVPKVPDTDNLKFIAGDMF 798
            +++ +AQ H++  +Y + +S SLKCA++L IP+++HKHGRP+TL QL+ ++ IN  K     +LMR LV++ FF   N           Y LT  S L LK+ P  ++P+V  + DP F NP H +S WF   + +    A +G  FW+ +A  P     +++AM+ DS+L A++  KD + + +G+ +LVDVGGG G+ A+ I EA P IKCTV+DLPHVV  +  TDNL +I GDMF
Sbjct:   13 EQLLQAQVHVWNHMYAFANSMSLKCAIQLGIPDILHKHGRPMTLSQLLQSIPINKEKTQCFQRLMRALVNSNFFIEEN---NSNNQEVCYWLTPASCLLLKEAPLTVTPLVQVVLDPTFTNPWHHMSEWFTHEKHATQFEAANGCTFWEKLANEPSKGRFFDEAMSCDSRLIAHVFTKDYKHVIEGIRTLVDVGGGNGTMAKAIVEAMPTIKCTVIDLPHVVAGLESTDNLNYIGGDMF 248          
BLAST of CX078059 vs. ExPASy Swiss-Prot
Match: 6OMT_COPJA ((RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 3.834e-45
Identity = 99/242 (40.91%), Postives = 141/242 (58.26%), Query Frame = 1
Query:   76 EGAKEMFEAQAHLFKIIYNYVSSTSLKCAVELDIPEVIHKHGRPITLPQLVSALEINPTKADGLFKLMRLLVHTGFFSTANVXXXXXXXXXXYALTLTSKLFLKDKPYCLSPVVLTLTDPVFVNPCHFLSRWFRDNELSAYETANDGTVFWDYMAKNPDFNSIYNQAMASDSQLA-NLIVKDCQPIFQGLGSLVDVGGGTGSFARIISEAFPGIKCTVLDLPHVVPKVPDTDNLKFIAGDMF 798
            E  K+   +QA L+  IY +  S  LKCAV+LD+  +IH  G  +TL +L S L   P   D L+++MR LVH   F+ A++          Y L   +K  +K    C+   +L +TD  F+ P H+L         +A+E A  GT  W YMA++P+ N ++N+AMA+DS+L  + +VK+C  IF G+ +LVDVGGGTG+  R I+ AFP IKCTV DLPHV+   P    +  +AGDMF
Sbjct:    2 EVKKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRLPSQPVNEDALYRVMRYLVHMKLFTKASIDGELR-----YGLAPPAKYLVKGWDKCMVGSILAITDKDFMAPWHYLKDGLSGESGTAFEKAL-GTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGNIFNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMF 237          
BLAST of CX078059 vs. ExPASy Swiss-Prot
Match: 4OMT_COPJA (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase OS=Coptis japonica PE=1 SV=1)

HSP 1 Score: 155.606 bits (392), Expect = 2.943e-37
Identity = 84/235 (35.74%), Postives = 134/235 (57.02%), Query Frame = 1
Query:   97 EAQAHLFKIIYNYVSSTSLKCAVELDIPEVIHKHGRPITLPQLVSALEINPTKADGLFKLMRLLVHTGFFSTANVXXXXXXXXXXYALTLTSKLFLKDKPYCLSPVVLTLTDPVFVNPCHFLSRWFRDNELSAYETANDGTVFWDYMAKNPDFNSIYNQAMASDSQL-ANLIVKDCQPIFQGLGSLVDVGGGTGSFARIISEAFPGIKCTVLDLPHVVPKVPDTDNLKFIAGDMF 798
            +AQAH++KIIY +  S  L+CAVEL I ++I  + +P+ L  L S L ++    D L++++R LV                    YAL   + L  ++    + P++L +T   F+ P H +     DN  +A+E A  G   W+Y+  +PD + ++N+ MA +++L  + ++   + +FQG+ SLVDVGGG G+  + IS+AFP IKCT+ DLPHV+    D  N++ I GDMF
Sbjct:   13 KAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLPVSDVNCDNLYRILRYLVKMEILRVEK----SDDGQKKYALEPIATLLSRNAKRSMVPMILGMTQKDFMTPWHSMKDGLSDNG-TAFEKAM-GMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDLPNIERIGGDMF 241          
BLAST of CX078059 vs. ExPASy Swiss-Prot
Match: ZRP4_MAIZE (O-methyltransferase ZRP4 OS=Zea mays GN=ZRP4 PE=2 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 7.248e-36
Identity = 89/248 (35.89%), Postives = 138/248 (55.65%), Query Frame = 1
Query:   73 NEGAKEMFEAQAHLFKIIYNYVSSTSLKCAVELDIPEVIHKHGRPITLPQLVSALEINPTKADGLFKLMRLLVHTGFFSTANVXXXXXXXXXX-YALTLTSKLFLKDKPYCLS--PVVLTLTDPVFVNPCHFLSRWFRDNELS--AYETANDGTVFWDYMAKNPDFNSIYNQAMASDSQL-ANLIVKDCQPIFQGLGSLVDVGGGTGSFARIISEAFPGIKCTVLDLPHVVPKVPDTDNLKFIAGDMF 798
            N   + + +AQ  L+   + ++ S +LK A+ L I + IH HG   +L Q++S + ++P++   L +LMR+L  T  F T  +           Y LT  S+L +  +   L+  P+   + DP  V+P   L  WF+ +EL          G   W+    +  F+++ N  +ASDSQL  ++ +K    +FQG+ SLVDVGGG G+ A+ IS+AFP +KC+VLDL HVV K P   +++FIAGDMF
Sbjct:    7 NSTDQSLLDAQLELWHTTFAFMKSMALKSAIHLRIADAIHLHGGAASLSQILSKVHLHPSRVSSLRRLMRVLTTTNVFGTQPLGGGSDDDSEPVYTLTPVSRLLIGSQSSQLAQTPLAAMVLDPTIVSPFSELGAWFQ-HELPDPCIFKHTHGRGIWELTKDDATFDALVNDGLASDSQLIVDVAIKQSAEVFQGISSLVDVGGGIGAAAQAISKAFPHVKCSVLDLAHVVAKAPTHTDVQFIAGDMF 253          
BLAST of CX078059 vs. ExPASy Swiss-Prot
Match: COOMT_COPJA (Columbamine O-methyltransferase OS=Coptis japonica PE=1 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 1.370e-26
Identity = 80/246 (32.52%), Postives = 122/246 (49.59%), Query Frame = 1
Query:   97 EAQAHLFKIIYNYVSSTSLKCAVELDIPEVIHKHGRPITLPQLVSALEINPTKADGLFKLMRLLVHTGFFSTANVXXXXXXXXXXYALTLTSKLFLKDKPYCLSPVVLTLTDPV-------FVNPCHFLSRWFRDNELSAYETANDGTVFWDYMAKNPDFNSIYNQAMASDSQ-----LANLIVKDCQPIFQGLGSLVDVGGGTGSFARIISEAFPGIKCTVLDLPHVVPKVPDTDNLKFIAGDMF 798
            +AQA ++K ++ +  +  L+  V L IP++IH +G P+TL QLV+ L +  T  D     MR LVH   F+   +          Y LT  SKL +      L+P V+  T P         +N       ++  N+ S YE              +P+ N I N AM S S      L + ++K+   +  G+ S+VDVGG +G  A+ I +AFP +KC+V+DL HV+ +V     L ++AGDMF
Sbjct:   14 KAQAQVWKHMFGFAETIMLRSTVSLGIPDIIHNNG-PVTLSQLVTHLPLKSTSIDRFHHFMRYLVHMQLFT---ISTDQITKEDKYELTPASKLLVHGHQKSLAPYVMLQTHPEEFSVWSHVINVLDGKKPYWESNDTSMYEKTEG----------DPEINEILNDAMTSHSTFMLPALVSGLMKE--NVLDGVASIVDVGGNSGVVAKGIVDAFPHVKCSVMDLNHVIERVIKNPKLDYVAGDMF 243          
BLAST of CX078059 vs. ExPASy Swiss-Prot
Match: COMT1_POPTM (Caffeic acid 3-O-methyltransferase 1 OS=Populus tremuloides GN=OMT1 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.794e-18
Identity = 70/243 (28.81%), Postives = 114/243 (46.91%), Query Frame = 1
Query:   88 EMFEAQAHLF--KIIYNYVSSTSLKCAVELDIPEVIHKHGRP--ITLPQLVSALEI-NPTKADGLFKLMRLLVHTGFFSTANVXXXXXXXXXXYALTLTSKLFLKDKP-YCLSPVVLTLTDPVFVNPCHFLSRWFRDNELSAYETANDGTVFWDYMAKNPDFNSIYNQAMASDSQLANLIVKDCQPIFQGLGSLVDVGGGTGSFARIISEAFPGIKCTVLDLPHVVPKVPDTDNLKFIAGDMF 798
            ++ + +AHLF  ++    V    LK A+ELD+ E++ K G    ++  ++ S L   NP     L +++RLL      + +            Y L    K   K++    +SP+ L   D V +   ++L     D  +  +  A   T F +Y   +P FN ++N+ M+  S +    + +    F+GL SLVDVGGGTG+    I   +P IK    DLPHV+   P    ++ + GDMF
Sbjct:   13 QVSDEEAHLFAMQLASASVLPMILKTAIELDLLEIMAKAGPGAFLSTSEIASHLPTKNPDAPVMLDRILRLLASYSILTCSLKDLPDGKVERLYGLAPVCKFLTKNEDGVSVSPLCLMNQDKVLMESWYYLKDAILDGGIP-FNKAYGMTAF-EYHGTDPRFNKVFNKGMSDHSTITMKKILETYKGFEGLTSLVDVGGGTGAVVNTIVSKYPSIKGINFDLPHVIEDAPSYPGVEHVGGDMF 253          
The following BLAST results are available for this feature:
BLAST of CX078059 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 34
Match NameE-valueIdentityDescription
7OMT9_MEDSA1.315e-5343.03Isoflavone-7-O-methyltransferase 9 OS=Medicago sat... [more]
7OMT8_MEDSA1.717e-5343.03Isoflavone-7-O-methyltransferase 8 OS=Medicago sat... [more]
7OMT6_MEDSA1.717e-5343.03Isoflavone-7-O-methyltransferase 6 OS=Medicago sat... [more]
CVMT1_OCIBA8.522e-5342.92Chavicol O-methyltransferase OS=Ocimum basilicum G... [more]
EOMT1_OCIBA7.215e-5242.26Eugenol O-methyltransferase OS=Ocimum basilicum GN... [more]
6OMT_COPJA3.834e-4540.91(RS)-norcoclaurine 6-O-methyltransferase OS=Coptis... [more]
4OMT_COPJA2.943e-3735.743'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltran... [more]
ZRP4_MAIZE7.248e-3635.89O-methyltransferase ZRP4 OS=Zea mays GN=ZRP4 PE=2 ... [more]
COOMT_COPJA1.370e-2632.52Columbamine O-methyltransferase OS=Coptis japonica... [more]
COMT1_POPTM1.794e-1828.81Caffeic acid 3-O-methyltransferase 1 OS=Populus tr... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS08_6F11_g Parent Washington Navel Orange Callus cDNA Library UCRCS08-2 Citrus sinensis cDNA clone UCRCS08-6F11-K22-1-6.g, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX078059 ID=CX078059; Name=CX078059; organism=Citrus sinensis; type=EST; length=799bp
CTCTCTTATCACTTTTCATTTTAACAAATTTCTGCATAACTATCGGTCAC
TGAAATTATGGATCCAATTCAAAATGAGGGAGCAAAAGAGATGTTTGAAG
CTCAAGCTCATCTGTTTAAAATTATATACAACTATGTGAGCTCAACTTCA
CTCAAGTGTGCAGTTGAGCTTGACATTCCGGAAGTAATTCACAAACACGG
ACGGCCCATCACTCTCCCTCAGTTAGTTTCGGCACTTGAGATTAATCCTA
CAAAAGCTGATGGCCTATTCAAGCTAATGAGGTTGTTGGTTCACACAGGC
TTCTTTTCAACAGCAAATGTTCAAAGCGCTCAGCAACAACAAGAAGAAGC
ATATGCTCTCACATTGACTTCCAAGCTGTTCCTCAAAGACAAGCCCTACT
GTTTATCACCAGTCGTTTTGACATTGACTGACCCAGTTTTTGTAAATCCA
TGCCATTTTTTGAGTCGGTGGTTCAGAGACAATGAGCTCTCAGCATATGA
AACTGCGAATGATGGAACGGTCTTTTGGGATTACATGGCAAAAAACCCTG
ATTTCAACAGCATCTACAATCAGGCCATGGCCAGTGATTCTCAACTGGCA
AACTTGATTGTTAAGGACTGCCAGCCAATTTTCCAGGGCTTAGGTTCGTT
AGTTGATGTAGGTGGTGGTACAGGATCCTTTGCCAGGATCATATCCGAGG
CATTTCCTGGCATAAAATGCACGGTACTTGATCTCCCACACGTTGTTCCC
AAAGTGCCAGACACCGACAACTTGAAATTCATAGCAGGCGACATGTTTC
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