DR908287

Overview
NameDR908287
Unique NameDR908287
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length672
Libraries
Library NameType
Citrus sinensis phloemcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of DR908287 vs. ExPASy Swiss-Prot
Match: CSLA2_ARATH (Glucomannan 4-beta-mannosyltransferase 2 OS=Arabidopsis thaliana GN=CSLA2 PE=2 SV=1)

HSP 1 Score: 367.851 bits (943), Expect = 2.797e-101
Identity = 172/218 (78.90%), Postives = 197/218 (90.37%), Query Frame = 1
Query:    1 IPESFQVSREDIAGQIGLIWELLKAPLIVPLLKLGVYICLAMSLMLFMERVYMGIVIILVKLFWQKPEKRYNWQPIEDDVELGSSNFPVVLIQIPMFNEKEVYKISIGAACGLSWPSDRLVIQVLDDSTDPAIKQMVEQECQRWAAKGINIRYQIRENRTGYKAGALKEGLKRSYVKHCEYVAIFDADFRPEPDYLRRAIPYLVQTLTLPLVQARWRF 654
            +PE+F   R +I GQ+G+IWEL+KAP+IVPLL+L VYICL MS+ML  ERVYMGIVI+LVKLFW+KP+KRY ++PI DD ELGSSNFPVVL+QIPMFNE+EVYK+SIGAACGLSWPSDRLVIQVLDDSTDP +KQMVE ECQRWA+KGINIRYQIRENR GYKAGALKEGLKRSYVKHCEYV IFDADF+PEPD+LRR+IP+L+    + LVQARWRF
Sbjct:   10 LPETFDGVRMEITGQLGMIWELVKAPVIVPLLQLAVYICLLMSVMLLCERVYMGIVIVLVKLFWKKPDKRYKFEPIHDDEELGSSNFPVVLVQIPMFNEREVYKLSIGAACGLSWPSDRLVIQVLDDSTDPTVKQMVEVECQRWASKGINIRYQIRENRVGYKAGALKEGLKRSYVKHCEYVVIFDADFQPEPDFLRRSIPFLMHNPNIALVQARWRF 227          
BLAST of DR908287 vs. ExPASy Swiss-Prot
Match: CSLA9_ARATH (Glucomannan 4-beta-mannosyltransferase 9 OS=Arabidopsis thaliana GN=CSLA9 PE=2 SV=1)

HSP 1 Score: 328.176 bits (840), Expect = 2.456e-89
Identity = 151/218 (69.27%), Postives = 190/218 (87.16%), Query Frame = 1
Query:    1 IPESFQVSREDIAGQIGLIWELLKAPLIVPLLKLGVYICLAMSLMLFMERVYMGIVIILVKLFWQKPEKRYNWQPIEDDVELGSSNFPVVLIQIPMFNEKEVYKISIGAACGLSWPSDRLVIQVLDDSTDPAIKQMVEQECQRWAAKGINIRYQIRENRTGYKAGALKEGLKRSYVKHCEYVAIFDADFRPEPDYLRRAIPYLVQTLTLPLVQARWRF 654
            IP+SF   R+DI  Q+ ++ + ++APLIVP L+LGVYICL MS+MLF+ERVYMGIVI LVKLF +KP+KR+ ++PI+DD+ELG+S +P+VLIQIPMFNE+EVY++SIGAACGLSWPSDR+VIQVLDDSTDP IK +VE EC RWA+KG+NI+Y+IR+NR GYKAGALKEG+K+SYVK C+YVAIFDADF+PE D+L R +PYL+    L LVQARW+F
Sbjct:   10 IPDSFMGYRDDITMQMSMVLDQIRAPLIVPALRLGVYICLTMSVMLFVERVYMGIVISLVKLFGRKPDKRFKYEPIKDDIELGNSAYPMVLIQIPMFNEREVYQLSIGAACGLSWPSDRIVIQVLDDSTDPTIKDLVEMECSRWASKGVNIKYEIRDNRNGYKAGALKEGMKKSYVKSCDYVAIFDADFQPEADFLWRTVPYLLHNPKLALVQARWKF 227          
BLAST of DR908287 vs. ExPASy Swiss-Prot
Match: CSLA1_ORYSJ (Glucomannan 4-beta-mannosyltransferase 1 OS=Oryza sativa subsp. japonica GN=CSLA1 PE=3 SV=1)

HSP 1 Score: 293.893 bits (751), Expect = 5.132e-79
Identity = 137/200 (68.50%), Postives = 168/200 (84.00%), Query Frame = 1
Query:   58 WELLKAPLIVPLLKLGVYICLAMSLMLFMERVYMGIVIILVKLFWQKPEKRYNWQPI-EDDVELGSSNFPVVLIQIPMFNEKEVYKISIGAACGLSWPSDRLVIQVLDDSTDPAIKQMVEQECQRWAAKGINIRYQIRENRTGYKAGALKEGLKRSYVKHCEYVAIFDADFRPEPDYLRRAIPYLVQTLTLPLVQARWRF 654
            W  ++AP+IVPLL+L V +CL MS++LF+ER+YM +VI  VK+  ++P++RY   PI +DD ELG+S FPVVLIQIPMFNE+EVY++SIGA CGLSWPSDRLV+QVLDDSTDP IK+MV  EC+RWA KG+NI YQIRENR GYKAGALKEG+K  YV+ CEYVAIFDADF+P+PD+LRR IP+LV    + LVQARWRF
Sbjct:   15 WSQVRAPVIVPLLRLAVAVCLTMSVLLFLERMYMAVVISGVKILRRRPDRRYRCDPIPDDDPELGTSAFPVVLIQIPMFNEREVYQLSIGAVCGLSWPSDRLVVQVLDDSTDPVIKEMVRIECERWAHKGVNITYQIRENRKGYKAGALKEGMKHGYVRECEYVAIFDADFQPDPDFLRRTIPFLVHNSDIALVQARWRF 214          
BLAST of DR908287 vs. ExPASy Swiss-Prot
Match: CSLA9_ORYSJ (Probable mannan synthase 9 OS=Oryza sativa subsp. japonica GN=CSLA9 PE=2 SV=1)

HSP 1 Score: 285.034 bits (728), Expect = 2.384e-76
Identity = 132/216 (61.11%), Postives = 173/216 (80.09%), Query Frame = 1
Query:   43 QIGLIWELLKAPLIVPLLKLGVYICLAMSLMLFMERVYMGIVIILVKLFWQKPEKRYNWQPI------EDDVELGSSN--FPVVLIQIPMFNEKEVYKISIGAACGLSWPSDRLVIQVLDDSTDPAIKQMVEQECQRWAAKGINIRYQIRENRTGYKAGALKEGLKRSYVKHCEYVAIFDADFRPEPDYLRRAIPYLVQTLTLPLVQARWRFGKLN 666
            QI  +WE +KAP++VPLL+L V  CLAMS+MLF+E+VYM +V++ V LF ++P++RY   PI       DD EL  +N  FP+VLIQIPM+NE+EVYK+SIGAACGLSWPSDR+++QVLDDSTDP IK+MV+ EC+RW +KG+ I+Y+IR+NR GYKAGAL+EG+K  YV+ C+YVAIFDADF+P+PD+L R IP+LV    + LVQARW+F   N
Sbjct:   11 QIAAMWEQVKAPVVVPLLRLSVAACLAMSVMLFVEKVYMSVVLVGVHLFGRRPDRRYRCDPIVAAGADNDDPELADANAAFPMVLIQIPMYNEREVYKLSIGAACGLSWPSDRVIVQVLDDSTDPVIKEMVQVECKRWESKGVRIKYEIRDNRVGYKAGALREGMKHGYVRDCDYVAIFDADFQPDPDFLARTIPFLVHNPDIALVQARWKFVNAN 226          
BLAST of DR908287 vs. ExPASy Swiss-Prot
Match: CSLA7_ARATH (Probable mannan synthase 7 OS=Arabidopsis thaliana GN=CSLA7 PE=2 SV=2)

HSP 1 Score: 283.878 bits (725), Expect = 5.311e-76
Identity = 125/209 (59.81%), Postives = 172/209 (82.30%), Query Frame = 1
Query:   28 EDIAGQIGLIWELLKAPLIVPLLKLGVYICLAMSLMLFMERVYMGIVIILVKLFWQKPEKRYNWQPIEDDVELGSSNFPVVLIQIPMFNEKEVYKISIGAACGLSWPSDRLVIQVLDDSTDPAIKQMVEQECQRWAAKGINIRYQIRENRTGYKAGALKEGLKRSYVKHCEYVAIFDADFRPEPDYLRRAIPYLVQTLTLPLVQARWRF 654
            EDI  +IGL W+L++A ++VP+ K  V +CL MS+M F+E +YMGIV++ VKLF +KPEK Y W+ +EDDVE GS+++P+VL+QIPM+NEKEV + SI AAC +SWPS+R++IQVLDDSTDPA K++V++EC RW+ +G+NI ++IR+NR GYKAGAL+EG++ SYVK C+YVAIFDADF+P+PD+L R +P+L+    L LVQ RW F
Sbjct:   41 EDIIARIGLWWQLIRAVVVVPVFKFLVLLCLVMSVMFFVEVMYMGIVVLYVKLFKRKPEKFYKWEAMEDDVECGSASYPMVLVQIPMYNEKEVCEQSIAAACKISWPSNRIIIQVLDDSTDPASKELVKKECDRWSKEGVNITFEIRDNRNGYKAGALREGMRHSYVKQCDYVAIFDADFQPDPDFLHRTVPFLIHNPKLALVQGRWEF 249          
BLAST of DR908287 vs. ExPASy Swiss-Prot
Match: CSLAF_ARATH (Probable mannan synthase 15 OS=Arabidopsis thaliana GN=CSLA15 PE=2 SV=2)

HSP 1 Score: 277.715 bits (709), Expect = 3.806e-74
Identity = 125/220 (56.82%), Postives = 171/220 (77.73%), Query Frame = 1
Query:    7 ESFQVSREDIAGQIGLIWELLKAPLIVPLLKLGVYICLAMSLMLFMERVYMGIVIILVKLFWQKPEKRYNWQPIEDDVELGSSNFPVVLIQIPMFNEKEVYKISIGAACGLSWPSDRLVIQVLDDSTDPAIKQMVEQECQRWAAKGINIRYQIRENRTGYKAGALKEGLKRSYVKHCEYVAIFDADFRPEPDYLRRAIPYLVQTLTLPLVQARWRFGKLN 666
            E+ + S + +   +  +W   +   IVPL K  V +CL +SL++F+E VYM +V++ VKLF +KPEK Y W+ +++D+ELG  N+P+VL+QIPM+NE+EV+++SIGAAC L+WPSDRL++QVLDDSTDPAI ++V  EC +WA+K INI Y+ RENR GYKAGALK G++ SYVK C+Y+AIFDADF+PEPDYL+RAIP+L+    + LVQARWRF   N
Sbjct:   26 ETLKASVDGVGINMSTMWRETRNVFIVPLFKCIVVMCLIISLLVFVESVYMNLVVLYVKLFNRKPEKVYKWEAMQEDMELGHQNYPMVLVQIPMYNEREVFELSIGAACRLTWPSDRLIVQVLDDSTDPAIMELVSMECTKWASKDININYERRENRNGYKAGALKHGMRHSYVKQCQYLAIFDADFQPEPDYLQRAIPFLIHNPEVALVQARWRFVNAN 245          
BLAST of DR908287 vs. ExPASy Swiss-Prot
Match: CSLA3_ARATH (Probable mannan synthase 3 OS=Arabidopsis thaliana GN=CSLA3 PE=2 SV=1)

HSP 1 Score: 271.166 bits (692), Expect = 3.563e-72
Identity = 127/217 (58.53%), Postives = 167/217 (76.96%), Query Frame = 1
Query:   19 VSREDIAGQIGLIWELLKAPLIVPLLKLGVYICLAMSLMLFMERVYMGIVIILVKLFWQKPEKRYNWQPI-EDDVELGSSNFPVVLIQIPMFNEKEVYKISIGAACGLSWPSDRLVIQVLDDSTDPAIKQMVEQECQRWAAKGINIRYQIRENRTGYKAGALKEGLKRSYVKHCEYVAIFDADFRPEPDYLRRAIPYLVQTLTLPLVQARWRFGKLN 666
            V R  I G+I  IW+  +  + +P+LK  V ICL MSL+LF+ERVYM IV++ VKL  + PEK + W+PI +DD+EL ++N+P+VLIQIPM+NEKEV ++SIGAAC LSWP DR+++QVLDDSTDPA K++V  EC +WA KGINI  +IR+NR GYKAGALK G+  +YVK CE+VAIFDADF+P+PD+L R IP+L+    + LVQ RW+F   N
Sbjct:   38 VVRSGIIGEIIYIWKQTRIFVFIPILKCLVTICLVMSLLLFIERVYMSIVVVFVKLLRRTPEKVHKWEPINDDDLELANTNYPMVLIQIPMYNEKEVCQLSIGAACRLSWPLDRMIVQVLDDSTDPASKELVNAECDKWARKGINIMSEIRDNRIGYKAGALKAGMMHNYVKQCEFVAIFDADFQPDPDFLERTIPFLIHNHEISLVQCRWKFVNAN 254          
BLAST of DR908287 vs. ExPASy Swiss-Prot
Match: CSLA1_CYATE (Mannan synthase 1 OS=Cyamopsis tetragonoloba GN=ManS PE=1 SV=1)

HSP 1 Score: 256.529 bits (654), Expect = 9.080e-68
Identity = 115/199 (57.79%), Postives = 159/199 (79.90%), Query Frame = 1
Query:   58 WELLKAPLIVPLLKLGVYICLAMSLMLFMERVYMGIVIILVKLFWQKPEKRYNWQPIEDDVELGSSNFPVVLIQIPMFNEKEVYKISIGAACGLSWPSDRLVIQVLDDSTDPAIKQMVEQECQRWAAKGINIRYQIRENRTGYKAGALKEGLKRSYVKHCEYVAIFDADFRPEPDYLRRAIPYLVQTLTLPLVQARWRF 654
            W+ ++AP+I+PLLKL V +C  MS+MLF+ERV M  VI++VK+  +K   +YN + ++  +E  S  +P+VLIQIPM+NEKEVYK+SIGA CGLSWP+DR ++QVLDDST+P ++++VE ECQ+W  KG+N++Y+ R NR GYKAGALKEGL++ YV+ CE+VAIFDADF+P+ D+L   IPYL++   L LVQARW+F
Sbjct:   24 WQSIRAPVIIPLLKLAVIVCSVMSIMLFVERVAMAAVILIVKVLRKKRYTKYNLEAMKQKLER-SKKYPMVLIQIPMYNEKEVYKLSIGAVCGLSWPADRFIVQVLDDSTNPVLRELVEMECQKWIQKGVNVKYENRRNRNGYKAGALKEGLEKQYVEDCEFVAIFDADFQPDADFLWNTIPYLLENPKLGLVQARWKF 221          
BLAST of DR908287 vs. ExPASy Swiss-Prot
Match: CSLAA_ARATH (Probable mannan synthase 10 OS=Arabidopsis thaliana GN=CSLA10 PE=2 SV=2)

HSP 1 Score: 254.988 bits (650), Expect = 2.642e-67
Identity = 114/195 (58.46%), Postives = 153/195 (78.46%), Query Frame = 1
Query:   82 IVPLLKLGVYICLAMSLMLFMERVYMGIVIILVKLFWQKPEKRYNWQPIEDDVELGSSNFPVVLIQIPMFNEKEVYKISIGAACGLSWPSDRLVIQVLDDSTDPAIKQMVEQECQRWAAKGINIRYQIRENRTGYKAGALKEGLKRSYVKHCEYVAIFDADFRPEPDYLRRAIPYLVQTLTLPLVQARWRFGKLN 666
            IVPL K  V  CL +SL++F+E +YM +V++ VK+F +KPEK Y W+ +++D+ELG   +P+VL+QIPM+NEKEV ++SIGAAC L WP DRL++QVLDDSTD  IK++V  EC +W +KG+NI+ + R+NR GYKAGALKEG+K +YVK C YV IFDADF+PEPDYL+ ++P+LV    + LVQARWRF   N
Sbjct:   62 IVPLFKCLVAFCLIISLLVFIEGIYMNLVVLYVKVFERKPEKVYRWEAMQEDIELGHETYPMVLVQIPMYNEKEVLQLSIGAACRLIWPLDRLIVQVLDDSTDQTIKELVNTECAKWESKGVNIKCERRDNRNGYKAGALKEGMKHNYVKLCNYVVIFDADFQPEPDYLQHSVPFLVHNPEVALVQARWRFMNAN 256          
BLAST of DR908287 vs. ExPASy Swiss-Prot
Match: CSLA1_ARATH (Probable mannan synthase 1 OS=Arabidopsis thaliana GN=CSLA1 PE=2 SV=1)

HSP 1 Score: 248.44 bits (633), Expect = 2.473e-65
Identity = 112/208 (53.85%), Postives = 158/208 (75.96%), Query Frame = 1
Query:   43 QIGLIWELLKAPLIVPLLKLGVYICLAMSLMLFMERVYMGIVIILVKLFWQKPEKRYNWQPIEDDVELGSSNFPVVLIQIPMFNEKEVYKISIGAACGLSWPSDRLVIQVLDDSTDPAIKQMVEQECQRWAAKGINIRYQIRENRTGYKAGALKEGLKRSYVKHCEYVAIFDADFRPEPDYLRRAIPYLVQTLTLPLVQARWRFGKLN 666
            Q+  +W   ++ +++P+ K  V +CL +S+++F E  YM  VI+ VKLF +KP K Y W+ +++DVE+G  N+P+VLIQIPM+NEKEV+++SI A C L WPS RLV+QV+DDSTDPA+++ V+ E  +W ++GINIR + R+NR GYKAGA+KE L +SYVK C++VA+FDADF+PEPDYL RA+P+LV    + LVQARW F   N
Sbjct:   51 QLQYLWTKTRSVVLLPVFKGLVVMCLVLSIIVFFESFYMNFVILFVKLFKRKPHKVYKWEAMQEDVEVGPDNYPMVLIQIPMYNEKEVFQLSIAAICSLVWPSSRLVVQVVDDSTDPAVREGVDVEIAKWQSQGINIRCERRDNRNGYKAGAMKEALTQSYVKQCDFVAVFDADFQPEPDYLIRAVPFLVHNPDVALVQARWIFVNAN 258          
The following BLAST results are available for this feature:
BLAST of DR908287 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 34
Match NameE-valueIdentityDescription
CSLA2_ARATH2.797e-10178.90Glucomannan 4-beta-mannosyltransferase 2 OS=Arabid... [more]
CSLA9_ARATH2.456e-8969.27Glucomannan 4-beta-mannosyltransferase 9 OS=Arabid... [more]
CSLA1_ORYSJ5.132e-7968.50Glucomannan 4-beta-mannosyltransferase 1 OS=Oryza ... [more]
CSLA9_ORYSJ2.384e-7661.11Probable mannan synthase 9 OS=Oryza sativa subsp. ... [more]
CSLA7_ARATH5.311e-7659.81Probable mannan synthase 7 OS=Arabidopsis thaliana... [more]
CSLAF_ARATH3.806e-7456.82Probable mannan synthase 15 OS=Arabidopsis thalian... [more]
CSLA3_ARATH3.563e-7258.53Probable mannan synthase 3 OS=Arabidopsis thaliana... [more]
CSLA1_CYATE9.080e-6857.79Mannan synthase 1 OS=Cyamopsis tetragonoloba GN=Ma... [more]
CSLAA_ARATH2.642e-6758.46Probable mannan synthase 10 OS=Arabidopsis thalian... [more]
CSLA1_ARATH2.473e-6553.85Probable mannan synthase 1 OS=Arabidopsis thaliana... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_16415 Citrus sinensis phloem Citrus sinensis cDNA clone VPE-08_F12 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DR908287 ID=DR908287; Name=DR908287; organism=Citrus sinensis; type=EST; length=672bp
ATACCAGAGTCATTCCAAGTGTCAAGAGAGGACATTGCAGGACAAATTGG
TTTGATTTGGGAACTACTCAAAGCCCCATTGATAGTCCCTTTGCTTAAAC
TCGGGGTCTACATTTGCTTGGCCATGTCACTCATGCTCTTCATGGAAAGG
GTTTACATGGGCATTGTTATAATCCTTGTTAAGCTGTTTTGGCAAAAACC
TGAGAAGCGTTACAATTGGCAGCCTATTGAAGACGATGTCGAATTGGGCA
GCTCTAATTTCCCCGTTGTTCTTATTCAAATCCCAATGTTCAATGAAAAA
GAGGTTTACAAAATCTCCATTGGAGCAGCTTGCGGACTCTCTTGGCCGTC
CGATCGTCTCGTGATCCAAGTCCTTGACGATTCAACTGACCCTGCCATCA
AGCAAATGGTGGAGCAAGAGTGCCAAAGATGGGCGGCCAAAGGCATTAAC
ATAAGGTACCAAATCAGAGAGAATCGCACGGGATACAAAGCTGGTGCTCT
CAAAGAAGGGCTCAAAAGAAGCTATGTTAAACATTGTGAATACGTGGCCA
TTTTCGACGCCGATTTCCGGCCGGAGCCCGATTATCTCCGACGTGCCATT
CCTTACTTAGTCCAAACTCTGACATTGCCCCTTGTTCAAGCTCGCTGGAG
ATTCGGTAAGCTAAATTTAATT
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