DR406094

Overview
NameDR406094
Unique NameDR406094
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length637
Libraries
Library NameType
Mature Juice Sac from Navel Orange in pTriplEx2 vectorcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UBC6_ASHGO (Ubiquitin-conjugating enzyme E2 6 OS=Ashbya gossypii GN=UBC6 PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.682e-12
Identity = 36/116 (31.03%), Postives = 62/116 (53.45%), Query Frame = 1
Query:  241 SQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICL---DILKEQWSPALTISKVLLSICSLLT 579
            S+ A KR++KE K + ++PP    A P  D++  W   I GP ++P+  G +  ++ FP DYPF PP +   T   +     N  +CL   D   + W+P+ +++ +L  + S +T
Sbjct:    3 SRQAYKRLSKEYKMMTENPPPYIVAAPKEDNILVWHYVITGPPETPYEDGQYHGTLVFPNDYPFNPPAIRMLTP--NGRFRENTRLCLSMSDYHPDTWNPSWSVATILTGLLSFMT 116          
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UBC17_CAEEL (Probable ubiquitin-conjugating enzyme protein 17 OS=Caenorhabditis elegans GN=ubc-17 PE=2 SV=2)

HSP 1 Score: 70.8626 bits (172), Expect = 6.393e-12
Identity = 47/120 (39.17%), Postives = 66/120 (55.00%), Query Frame = 1
Query:  253 SKRINKELKDLQKDPP--ASCSAGPVADD--MFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTK-----VFHPNINSNGSICLDIL-------KEQWSPALTISKVLLSI 564
            +KRI KEL  +    P  AS S     D+  +   +  I GP D+P+A G+F   I FP  YPF PPK AF T       F+PN+ ++G ICL IL       +E+W+P  ++ +VL+SI
Sbjct:  411 TKRIAKELASIANALPLNASNSIYVCYDEGRVDIIKVLISGPDDTPYANGLFEFDIFFPTGYPFSPPKCAFLTTGSGNVRFNPNLYNDGKICLSILGTWEGRPEEKWNPYCSLMQVLVSI 530          
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UB2J2_BOVIN (Ubiquitin-conjugating enzyme E2 J2 OS=Bos taurus GN=UBE2J2 PE=2 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.424e-11
Identity = 34/127 (26.77%), Postives = 64/127 (50.39%), Query Frame = 1
Query:  220 SDGLLRNSQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICL---DILKEQWSPALTISKVLLSICSLLTDPNP 591
            S+ + R    A++R+ ++   ++KDP     A P+  ++  W   + GP  +P+ GG +   + FP ++PFKPP +   T   +     N  +CL   D   + W+PA ++S +L  + S + +  P
Sbjct:    3 SNSVKRAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITP--NGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGP 127          
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: Y1A1_ENCCU (Probable ubiquitin-conjugating enzyme E2 ECU01_1010 OS=Encephalitozoon cuniculi GN=ECU01_1010 PE=1 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 1.860e-11
Identity = 34/110 (30.91%), Postives = 54/110 (49.09%), Query Frame = 1
Query:  343 WQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDIL--------------KEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYK 630
            W        DS +AG +    + FP  YP +PP + F +K+FHPNI  +G +C+ IL              K++W+P   I  +++SI  +L  PN   P   + + MY+
Sbjct:   68 WDIYFTLGGDSLYAGRILKAVMKFPSSYPLRPPTLKFVSKMFHPNIYEDGKMCISILEEDKQQDSSVFGDPKDKWTPVQNIRTIVMSIVVILNSPNISSPANVDASVMYR 177          
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UB2J2_MOUSE (Ubiquitin-conjugating enzyme E2 J2 OS=Mus musculus GN=Ube2j2 PE=1 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 4.144e-11
Identity = 32/117 (27.35%), Postives = 60/117 (51.28%), Query Frame = 1
Query:  250 ASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICL---DILKEQWSPALTISKVLLSICSLLTDPNP 591
            A++R+ ++   ++KDP     A P+  ++  W   + GP  +P+ GG +   + FP ++PFKPP +   T   +     N  +CL   D   + W+PA ++S +L  + S + +  P
Sbjct:   13 ATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITP--NGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGP 127          
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UB2J2_HUMAN (Ubiquitin-conjugating enzyme E2 J2 OS=Homo sapiens GN=UBE2J2 PE=1 SV=3)

HSP 1 Score: 68.1662 bits (165), Expect = 4.144e-11
Identity = 32/117 (27.35%), Postives = 60/117 (51.28%), Query Frame = 1
Query:  250 ASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICL---DILKEQWSPALTISKVLLSICSLLTDPNP 591
            A++R+ ++   ++KDP     A P+  ++  W   + GP  +P+ GG +   + FP ++PFKPP +   T   +     N  +CL   D   + W+PA ++S +L  + S + +  P
Sbjct:   13 ATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITP--NGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGP 127          
The following BLAST results are available for this feature:
BLAST of DR406094 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 266
Match NameE-valueIdentityDescription
UBC6_ASHGO1.682e-1231.03Ubiquitin-conjugating enzyme E2 6 OS=Ashbya gossyp... [more]
UBC17_CAEEL6.393e-1239.17Probable ubiquitin-conjugating enzyme protein 17 O... [more]
UB2J2_BOVIN1.424e-1126.77Ubiquitin-conjugating enzyme E2 J2 OS=Bos taurus G... [more]
Y1A1_ENCCU1.860e-1130.91Probable ubiquitin-conjugating enzyme E2 ECU01_101... [more]
UB2J2_MOUSE4.144e-1127.35Ubiquitin-conjugating enzyme E2 J2 OS=Mus musculus... [more]
UB2J2_HUMAN4.144e-1127.35Ubiquitin-conjugating enzyme E2 J2 OS=Homo sapiens... [more]

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Properties
Property NameValue
Genbank descriptionCSAJ-PNP1248L04 Mature Juice Sac from Navel Orange in pTriplEx2 vector Citrus sinensis cDNA clone CSAJ-PNP1248L04 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DR406094 ID=DR406094; Name=DR406094; organism=Citrus sinensis; type=EST; length=637bp
TTTTTTTTTTTAAATTTCNNAATTATTTCATTAATTTTTAAAATTCCCCA
ATAATCACGCCTTCGTCCCTGATTTTGGTTTGAATTTATAAATAAAGGTT
GTTGCTTCAAATCTGTCGGTACAATTTTTGTACTTTTTAGAATTAGGGTT
AGGGTTTGTGATCGTGTTTTCGATTCTGTTTGCTTTTAAGATATAACCCT
CGAAGTACGGCACTGTTTCTCTGATGGTTTGTTGAGAAATTCCCAGATGG
CTTCAAAACGGATCAACAAGGAGTTGAAGGACCTACAGAAGGACCCTCCT
GCTTCTTGTAGCGCTGGCCCTGTTGCTGATGACATGTTCCATTGGCAAGC
AACAATTATGGGCCCATCAGACAGCCCATTTGCTGGGGGTGTATTTCTTG
TATCCATTCATTTCCCACCTGATTATCCATTCAAGCCACCGAAGGTGGCC
TTCCGTACTAAGGTTTTCCACCCTAACATCAATAGCAATGGTAGCATCTG
TCTTGACATTCTCAAGGAGCAGTGGAGCCCTGCTTTGACAATATCCAAGG
TACTTCTGTCAATTTGCTCACTACTTACTGATCCGAACCCAGATGACCCA
CTTGTGCCTGAGATTGCTCACATGTACAAGACTGACA
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