DR406094

Overview
NameDR406094
Unique NameDR406094
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length637
Libraries
Library NameType
Mature Juice Sac from Navel Orange in pTriplEx2 vectorcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UB2D1_HUMAN (Ubiquitin-conjugating enzyme E2 D1 OS=Homo sapiens GN=UBE2D1 PE=1 SV=1)

HSP 1 Score: 229.95 bits (585), Expect = 8.234e-60
Identity = 102/130 (78.46%), Postives = 116/130 (89.23%), Query Frame = 1 
Query:  247 MASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTD 636
            MA KRI KEL DLQ+DPPA CSAGPV DD+FHWQATIMGP DS + GGVF +++HFP DYPFKPPK+AF TK++HPNINSNGSICLDIL+ QWSPALT+SKVLLSICSLL DPNPDDPLVP+IA +YK+D
Sbjct:    1 MALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYPFKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQIYKSD 130
[Close]
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UB2D1_BOVIN (Ubiquitin-conjugating enzyme E2 D1 OS=Bos taurus GN=UBE2D1 PE=2 SV=1)

HSP 1 Score: 229.95 bits (585), Expect = 8.234e-60
Identity = 102/130 (78.46%), Postives = 116/130 (89.23%), Query Frame = 1 
Query:  247 MASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTD 636
            MA KRI KEL DLQ+DPPA CSAGPV DD+FHWQATIMGP DS + GGVF +++HFP DYPFKPPK+AF TK++HPNINSNGSICLDIL+ QWSPALT+SKVLLSICSLL DPNPDDPLVP+IA +YK+D
Sbjct:    1 MALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYPFKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQIYKSD 130
[Close]
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UBC12_ARATH (Probable ubiquitin-conjugating enzyme E2 12 OS=Arabidopsis thaliana GN=UBC12 PE=2 SV=1)

HSP 1 Score: 223.016 bits (567), Expect = 1.007e-57
Identity = 104/131 (79.39%), Postives = 119/131 (90.84%), Query Frame = 1 
Query:  247 MASKRINKELKDLQKDPPASCSAGPVAD-DMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTD 636
            MASKRI++EL+D+Q+ PPA+CSAGPVA+ D+FHWQATIMGP DSP++GGVF VSI F  DYPFKPPKV F+TKV+HPNI+S GSICLDILKEQWSPA T SKVLLSICSLLTDPNP+DPLVPEIAH+YK D
Sbjct:    1 MASKRISRELRDMQRHPPANCSAGPVAEEDIFHWQATIMGPHDSPYSGGVFTVSIDFSSDYPFKPPKVNFKTKVYHPNIDSKGSICLDILKEQWSPAPTTSKVLLSICSLLTDPNPNDPLVPEIAHLYKVD 131
[Close]
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: U2D2B_RAT (Ubiquitin-conjugating enzyme E2 D2B OS=Rattus norvegicus GN=Ube2d2b PE=2 SV=1)

HSP 1 Score: 221.476 bits (563), Expect = 2.929e-57
Identity = 100/130 (76.92%), Postives = 114/130 (87.69%), Query Frame = 1 
Query:  247 MASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTD 636
            MA KRI+KEL DL +DPPA CSAGPV +DMFHWQATIMGP+DSP+ GG F ++I FP +YPFKPPKV F T+++HPN+NSNGSICLDIL+ QWSPALTISKVLLSI SLL DPNPDDPLVPEIA +YKTD
Sbjct:    1 MALKRIHKELNDLAQDPPAQCSAGPVGEDMFHWQATIMGPNDSPYQGGAFFLTIDFPTEYPFKPPKVEFTTRIYHPNVNSNGSICLDILRSQWSPALTISKVLLSISSLLCDPNPDDPLVPEIAQIYKTD 130
[Close]
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: U2D2B_MOUSE (Ubiquitin-conjugating enzyme E2 D2B OS=Mus musculus GN=Ube2d2b PE=2 SV=1)

HSP 1 Score: 221.476 bits (563), Expect = 2.929e-57
Identity = 100/130 (76.92%), Postives = 114/130 (87.69%), Query Frame = 1 
Query:  247 MASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTD 636
            MA KRI+KEL DL +DPPA CSAGPV +DMFHWQATIMGP+DSP+ GG F ++I FP +YPFKPPKV F T+++HPN+NSNGSICLDIL+ QWSPALTISKVLLSI SLL DPNPDDPLVPEIA +YKTD
Sbjct:    1 MALKRIHKELNDLAQDPPAQCSAGPVGEDMFHWQATIMGPNDSPYQGGAFFLTIDFPTEYPFKPPKVEFTTRIYHPNVNSNGSICLDILRSQWSPALTISKVLLSISSLLCDPNPDDPLVPEIAQIYKTD 130
[Close]
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UB2E1_MOUSE (Ubiquitin-conjugating enzyme E2 E1 OS=Mus musculus GN=Ube2e1 PE=2 SV=1)

HSP 1 Score: 180.644 bits (457), Expect = 5.729e-45
Identity = 86/137 (62.77%), Postives = 105/137 (76.64%), Query Frame = 1 
Query:  235 RNSQMAS---KRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTD 636
            +NS++ S   KRI KEL D+  DPP +CSAGP  D+++ W++TI+GP  S + GGVF + I F P+YPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y T+
Sbjct:   40 KNSKLLSTSAKRIQKELADITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFTPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTN 176
[Close]
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UB2E1_HUMAN (Ubiquitin-conjugating enzyme E2 E1 OS=Homo sapiens GN=UBE2E1 PE=1 SV=1)

HSP 1 Score: 180.644 bits (457), Expect = 5.729e-45
Identity = 86/137 (62.77%), Postives = 105/137 (76.64%), Query Frame = 1 
Query:  235 RNSQMAS---KRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTD 636
            +NS++ S   KRI KEL D+  DPP +CSAGP  D+++ W++TI+GP  S + GGVF + I F P+YPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y T+
Sbjct:   40 KNSKLLSTSAKRIQKELADITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFTPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTN 176
[Close]
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UB2E2_MOUSE (Ubiquitin-conjugating enzyme E2 E2 OS=Mus musculus GN=Ube2e2 PE=2 SV=1)

HSP 1 Score: 179.874 bits (455), Expect = 9.772e-45
Identity = 83/129 (64.34%), Postives = 101/129 (78.29%), Query Frame = 1 
Query:  250 ASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTD 636
            ++KRI KEL ++  DPP +CSAGP  D+++ W++TI+GP  S + GGVF + I F PDYPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y T+
Sbjct:   56 SAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTN 184
[Close]
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UB2E2_HUMAN (Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens GN=UBE2E2 PE=1 SV=1)

HSP 1 Score: 179.874 bits (455), Expect = 9.772e-45
Identity = 83/129 (64.34%), Postives = 101/129 (78.29%), Query Frame = 1 
Query:  250 ASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTD 636
            ++KRI KEL ++  DPP +CSAGP  D+++ W++TI+GP  S + GGVF + I F PDYPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y T+
Sbjct:   56 SAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTN 184
[Close]
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UBCD2_DROME (Ubiquitin-conjugating enzyme E2-24 kDa OS=Drosophila melanogaster GN=UbcD2 PE=1 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 1.276e-44
Identity = 82/126 (65.08%), Postives = 99/126 (78.57%), Query Frame = 1 
Query:  250 ASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMY 627
            ++KRI KEL ++  DPP +CSAGP  D+++ W +TI+GP  S + GGVF + IHF P+YPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y
Sbjct:   87 SAKRIQKELAEITLDPPPNCSAGPKGDNLYEWVSTILGPPGSVYEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQY 212
The following BLAST results are available for this feature:
BLAST of DR406094 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 266
Match NameE-valueIdentityDescription
UB2D1_HUMAN8.234e-6078.46Ubiquitin-conjugating enzyme E2 D1 OS=Homo sapiens... [more]
UB2D1_BOVIN8.234e-6078.46Ubiquitin-conjugating enzyme E2 D1 OS=Bos taurus G... [more]
UBC12_ARATH1.007e-5779.39Probable ubiquitin-conjugating enzyme E2 12 OS=Ara... [more]
U2D2B_RAT2.929e-5776.92Ubiquitin-conjugating enzyme E2 D2B OS=Rattus norv... [more]
U2D2B_MOUSE2.929e-5776.92Ubiquitin-conjugating enzyme E2 D2B OS=Mus musculu... [more]
UB2E1_MOUSE5.729e-4562.77Ubiquitin-conjugating enzyme E2 E1 OS=Mus musculus... [more]
UB2E1_HUMAN5.729e-4562.77Ubiquitin-conjugating enzyme E2 E1 OS=Homo sapiens... [more]
UB2E2_MOUSE9.772e-4564.34Ubiquitin-conjugating enzyme E2 E2 OS=Mus musculus... [more]
UB2E2_HUMAN9.772e-4564.34Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens... [more]
UBCD2_DROME1.276e-4465.08Ubiquitin-conjugating enzyme E2-24 kDa OS=Drosophi... [more]

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Properties
Property NameValue
Genbank descriptionCSAJ-PNP1248L04 Mature Juice Sac from Navel Orange in pTriplEx2 vector Citrus sinensis cDNA clone CSAJ-PNP1248L04 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DR406094 ID=DR406094; Name=DR406094; organism=Citrus sinensis; type=EST; length=637bp
TTTTTTTTTTTAAATTTCNNAATTATTTCATTAATTTTTAAAATTCCCCA
ATAATCACGCCTTCGTCCCTGATTTTGGTTTGAATTTATAAATAAAGGTT
GTTGCTTCAAATCTGTCGGTACAATTTTTGTACTTTTTAGAATTAGGGTT
AGGGTTTGTGATCGTGTTTTCGATTCTGTTTGCTTTTAAGATATAACCCT
CGAAGTACGGCACTGTTTCTCTGATGGTTTGTTGAGAAATTCCCAGATGG
CTTCAAAACGGATCAACAAGGAGTTGAAGGACCTACAGAAGGACCCTCCT
GCTTCTTGTAGCGCTGGCCCTGTTGCTGATGACATGTTCCATTGGCAAGC
AACAATTATGGGCCCATCAGACAGCCCATTTGCTGGGGGTGTATTTCTTG
TATCCATTCATTTCCCACCTGATTATCCATTCAAGCCACCGAAGGTGGCC
TTCCGTACTAAGGTTTTCCACCCTAACATCAATAGCAATGGTAGCATCTG
TCTTGACATTCTCAAGGAGCAGTGGAGCCCTGCTTTGACAATATCCAAGG
TACTTCTGTCAATTTGCTCACTACTTACTGATCCGAACCCAGATGACCCA
CTTGTGCCTGAGATTGCTCACATGTACAAGACTGACA
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