DR406094

Overview
NameDR406094
Unique NameDR406094
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length637
Libraries
Library NameType
Mature Juice Sac from Navel Orange in pTriplEx2 vectorcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UBC2_DICDI (Ubiquitin-conjugating enzyme E2 2 OS=Dictyostelium discoideum GN=ubc2 PE=3 SV=2)

HSP 1 Score: 129.413 bits (324), Expect = 1.515e-29
Identity = 60/130 (46.15%), Postives = 84/130 (64.62%), Query Frame = 1
Query:  241 SQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYK 630
            S  A +R+ ++ K LQ DPPA  S  P+ +++  WQA I GP DS + G  F +S+ F  +YP   P V F +K+FHPN+ ++GSICLDIL+ QWSP   I+ +L SI SLL DPNP+ P   E A +++
Sbjct:    2 STAARRRLMRDFKRLQSDPPAGISGAPLENNILMWQAVIFGPDDSIWEGATFKLSLQFSEEYPNDAPTVKFLSKMFHPNVYADGSICLDILQNQWSPIYDIAAILTSIQSLLCDPNPNSPANSESARLFR 131          
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UBC2_DEBHA (Ubiquitin-conjugating enzyme E2 2 OS=Debaryomyces hansenii GN=UBC2 PE=3 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 1.515e-29
Identity = 57/130 (43.85%), Postives = 85/130 (65.38%), Query Frame = 1
Query:  241 SQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYK 630
            S  A +R+ ++ K +Q+D P+  SA P+ D++  W A I+GP+D+PF  G F + + F   YP KPP V F +++FHPN+ ++G +CLDIL+ +WSP   +S +L SI SLL DPN   P   E A++YK
Sbjct:    2 STPAKRRLMRDFKRMQQDAPSGVSASPLPDNVMSWNAVIIGPADTPFEDGTFRLILQFDEQYPNKPPSVKFISEMFHPNVYASGELCLDILQNRWSPTYDVSSILTSIQSLLNDPNISSPANVEAANLYK 131          
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UBC13_YEAST (Ubiquitin-conjugating enzyme E2 13 OS=Saccharomyces cerevisiae GN=UBC13 PE=1 SV=1)

HSP 1 Score: 129.028 bits (323), Expect = 1.978e-29
Identity = 61/121 (50.41%), Postives = 81/121 (66.94%), Query Frame = 1
Query:  256 KRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIA 618
            KRI KE + L  DP    +A P  D++ ++Q TI GP  SP+  G+F + ++ P DYP + PKV F TK++HPNI+  G ICLD+LK  WSPAL I  VLLSI +LL  PNP+DPL  ++A
Sbjct:    6 KRIIKETEKLVSDPVPGITAEPHDDNLRYFQVTIEGPEQSPYEDGIFELELYLPDDYPMEAPKVRFLTKIYHPNIDRLGRICLDVLKTNWSPALQIRTVLLSIQALLASPNPNDPLANDVA 126          
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UBE2N_RAT (Ubiquitin-conjugating enzyme E2 N OS=Rattus norvegicus GN=Ube2n PE=1 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 4.407e-29
Identity = 60/127 (47.24%), Postives = 82/127 (64.57%), Query Frame = 1
Query:  256 KRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTD 636
            +RI KE + L  +P     A P   +  ++   I GP DSPF GG F + +  P +YP   PKV F TK++HPN++  G ICLDILK++WSPAL I  VLLSI +LL+ PNPDDPL  ++A  +K++
Sbjct:    6 RRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKSN 132          
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UBC13_SCHPO (Ubiquitin-conjugating enzyme E2 13 OS=Schizosaccharomyces pombe GN=ubc13 PE=1 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 1.282e-28
Identity = 58/125 (46.40%), Postives = 82/125 (65.60%), Query Frame = 1
Query:  256 KRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYK 630
            KRI KE++ L +DPP    A P  D++ +++ T+ GP  S + GG F + +  P +YP  PP V F TK++HPN++  G ICL  LK+ WSPAL I  VLLSI +L+  PNPDDPL  ++A ++K
Sbjct:    5 KRIIKEIETLTRDPPPGIVAAPTEDNLRYFKITMEGPQQSAYEGGKFHLELFLPDEYPMMPPNVRFLTKIYHPNVDKLGRICLSTLKKDWSPALQIRTVLLSIQALMGAPNPDDPLDNDVAKIWK 129          
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UBC2_CANGA (Ubiquitin-conjugating enzyme E2 2 OS=Candida glabrata GN=UBC2 PE=3 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 1.675e-28
Identity = 55/130 (42.31%), Postives = 84/130 (64.62%), Query Frame = 1
Query:  241 SQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYK 630
            S  A +R+ ++ K +++D P   SA P+ D++  W A I+GP+D+P+  G F + + F  DYP KPP V F +++FHPN+ +NG ICLDIL+ +W+P   ++ +L SI SL  DPNP  P   E A ++K
Sbjct:    2 STPARRRLMRDFKRMKEDSPPGVSASPLPDNVMVWNAMIIGPADTPYEDGTFRLLLEFDEDYPNKPPHVKFLSEMFHPNVYANGEICLDILQNRWTPTYDVASILTSIQSLFNDPNPASPANVEAATLFK 131          
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UBC1_YEAST (Ubiquitin-conjugating enzyme E2-24 kDa OS=Saccharomyces cerevisiae GN=UBC1 PE=1 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 1.675e-28
Identity = 61/130 (46.92%), Postives = 87/130 (66.92%), Query Frame = 1
Query:  253 SKRINKELKDLQKDPPASCSAGPVAD-DMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINS-NGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTD 636
            +KRI KE++ ++ DP A  +   V++ D+ H + T +GP  +P+ GG F+V I  P +YPFKPPK+ F TKV+HPNI+S  G+ICLDILK  WSP +T+   L+S+ +LL  P P+DP   E+A  Y  D
Sbjct:    4 AKRIMKEIQAVKDDPAAHITLEFVSESDIHHLKGTFLGPPGTPYEGGKFVVDIEVPMEYPFKPPKMQFDTKVYHPNISSVTGAICLDILKNAWSPVITLKSALISLQALLQSPEPNDPQDAEVAQHYLRD 133          
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UBC2_YEAST (Ubiquitin-conjugating enzyme E2 2 OS=Saccharomyces cerevisiae GN=RAD6 PE=1 SV=1)

HSP 1 Score: 124.405 bits (311), Expect = 4.872e-28
Identity = 54/130 (41.54%), Postives = 84/130 (64.62%), Query Frame = 1
Query:  241 SQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYK 630
            S  A +R+ ++ K +++D P   SA P+ D++  W A I+GP+D+P+  G F + + F  +YP KPP V F +++FHPN+ +NG ICLDIL+ +W+P   ++ +L SI SL  DPNP  P   E A ++K
Sbjct:    2 STPARRRLMRDFKRMKEDAPPGVSASPLPDNVMVWNAMIIGPADTPYEDGTFRLLLEFDEEYPNKPPHVKFLSEMFHPNVYANGEICLDILQNRWTPTYDVASILTSIQSLFNDPNPASPANVEAATLFK 131          
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UBC2_ASHGO (Ubiquitin-conjugating enzyme E2 2 OS=Ashbya gossypii GN=UBC2 PE=3 SV=1)

HSP 1 Score: 124.405 bits (311), Expect = 4.872e-28
Identity = 54/130 (41.54%), Postives = 84/130 (64.62%), Query Frame = 1
Query:  241 SQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYK 630
            S  A +R+ ++ K +++D P   SA P+ D++  W A I+GP+D+P+  G F + + F  +YP KPP V F +++FHPN+ +NG ICLDIL+ +W+P   ++ +L SI SL  DPNP  P   E A ++K
Sbjct:    2 STPARRRLMRDFKRMKEDAPPGVSASPLPDNVMVWNAMIIGPADTPYEDGTFRLLLEFDEEYPNKPPHVKFLSEMFHPNVYANGEICLDILQNRWTPTYDVASILTSIQSLFNDPNPASPANVEAATLFK 131          
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UBE2C_DICDI (Probable ubiquitin-conjugating enzyme E2 C OS=Dictyostelium discoideum GN=ube2c PE=3 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 8.311e-28
Identity = 56/117 (47.86%), Postives = 78/117 (66.67%), Query Frame = 1
Query:  253 SKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPL 603
            SKR+  EL +L   P    SA P  D++F+W  TI G  D+ +    F +S+ FP DYP+KPP V F T+ FHPN+++NG+ICLDILK++WSP   +  +L+SI SLL +PN + PL
Sbjct:    8 SKRLQSELMNLMMGPSPGISAFPDGDNIFNWIGTIQGAKDTVYEQMEFKMSLKFPTDYPYKPPTVKFETQCFHPNVDNNGNICLDILKDKWSPVYNVRSLLISIQSLLGEPNNESPL 124          
The following BLAST results are available for this feature:
BLAST of DR406094 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 266
Match NameE-valueIdentityDescription
UBC2_DICDI1.515e-2946.15Ubiquitin-conjugating enzyme E2 2 OS=Dictyostelium... [more]
UBC2_DEBHA1.515e-2943.85Ubiquitin-conjugating enzyme E2 2 OS=Debaryomyces ... [more]
UBC13_YEAST1.978e-2950.41Ubiquitin-conjugating enzyme E2 13 OS=Saccharomyce... [more]
UBE2N_RAT4.407e-2947.24Ubiquitin-conjugating enzyme E2 N OS=Rattus norveg... [more]
UBC13_SCHPO1.282e-2846.40Ubiquitin-conjugating enzyme E2 13 OS=Schizosaccha... [more]
UBC2_CANGA1.675e-2842.31Ubiquitin-conjugating enzyme E2 2 OS=Candida glabr... [more]
UBC1_YEAST1.675e-2846.92Ubiquitin-conjugating enzyme E2-24 kDa OS=Saccharo... [more]
UBC2_YEAST4.872e-2841.54Ubiquitin-conjugating enzyme E2 2 OS=Saccharomyces... [more]
UBC2_ASHGO4.872e-2841.54Ubiquitin-conjugating enzyme E2 2 OS=Ashbya gossyp... [more]
UBE2C_DICDI8.311e-2847.86Probable ubiquitin-conjugating enzyme E2 C OS=Dict... [more]

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Properties
Property NameValue
Genbank descriptionCSAJ-PNP1248L04 Mature Juice Sac from Navel Orange in pTriplEx2 vector Citrus sinensis cDNA clone CSAJ-PNP1248L04 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DR406094 ID=DR406094; Name=DR406094; organism=Citrus sinensis; type=EST; length=637bp
TTTTTTTTTTTAAATTTCNNAATTATTTCATTAATTTTTAAAATTCCCCA
ATAATCACGCCTTCGTCCCTGATTTTGGTTTGAATTTATAAATAAAGGTT
GTTGCTTCAAATCTGTCGGTACAATTTTTGTACTTTTTAGAATTAGGGTT
AGGGTTTGTGATCGTGTTTTCGATTCTGTTTGCTTTTAAGATATAACCCT
CGAAGTACGGCACTGTTTCTCTGATGGTTTGTTGAGAAATTCCCAGATGG
CTTCAAAACGGATCAACAAGGAGTTGAAGGACCTACAGAAGGACCCTCCT
GCTTCTTGTAGCGCTGGCCCTGTTGCTGATGACATGTTCCATTGGCAAGC
AACAATTATGGGCCCATCAGACAGCCCATTTGCTGGGGGTGTATTTCTTG
TATCCATTCATTTCCCACCTGATTATCCATTCAAGCCACCGAAGGTGGCC
TTCCGTACTAAGGTTTTCCACCCTAACATCAATAGCAATGGTAGCATCTG
TCTTGACATTCTCAAGGAGCAGTGGAGCCCTGCTTTGACAATATCCAAGG
TACTTCTGTCAATTTGCTCACTACTTACTGATCCGAACCCAGATGACCCA
CTTGTGCCTGAGATTGCTCACATGTACAAGACTGACA
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